Oceanobacillus limi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3982 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0FAG6|A0A1I0FAG6_9BACI Catechol 2 3-dioxygenase OS=Oceanobacillus limi OX=930131 GN=SAMN05216389_11482 PE=4 SV=1
MM1 pKa = 7.5TKK3 pKa = 10.11TSITKK8 pKa = 8.94WLFALMLAMVLVLAACSDD26 pKa = 3.96DD27 pKa = 3.63TSSDD31 pKa = 3.5DD32 pKa = 5.31SSNDD36 pKa = 3.31DD37 pKa = 3.89PNTEE41 pKa = 3.62EE42 pKa = 4.91SSNNEE47 pKa = 3.5NDD49 pKa = 3.64GNDD52 pKa = 3.62AEE54 pKa = 4.38EE55 pKa = 4.53EE56 pKa = 4.23EE57 pKa = 4.32PAEE60 pKa = 4.8DD61 pKa = 4.7DD62 pKa = 3.69GLYY65 pKa = 10.83SIDD68 pKa = 4.61DD69 pKa = 3.81FSMDD73 pKa = 3.08KK74 pKa = 10.7TNAGEE79 pKa = 4.29AIEE82 pKa = 5.54GGTLNYY88 pKa = 10.69GLVSDD93 pKa = 4.4TVFEE97 pKa = 4.28GTLNFNFYY105 pKa = 10.66SGNPDD110 pKa = 2.94WEE112 pKa = 4.5VISWFDD118 pKa = 3.91EE119 pKa = 4.05PLLYY123 pKa = 10.38TDD125 pKa = 5.35ANYY128 pKa = 9.61TYY130 pKa = 9.78TNEE133 pKa = 4.05GPATYY138 pKa = 9.65EE139 pKa = 3.77VSEE142 pKa = 5.12DD143 pKa = 3.35GLTFTLTIKK152 pKa = 10.95DD153 pKa = 3.49GVNWHH158 pKa = 7.14DD159 pKa = 4.06GEE161 pKa = 4.53PVTAEE166 pKa = 3.6DD167 pKa = 2.88WAYY170 pKa = 8.61SYY172 pKa = 11.13EE173 pKa = 4.63VIGHH177 pKa = 6.93PDD179 pKa = 3.4YY180 pKa = 11.49TGVRR184 pKa = 11.84YY185 pKa = 10.14NDD187 pKa = 3.85DD188 pKa = 3.41FTNVVGMEE196 pKa = 4.15EE197 pKa = 4.25YY198 pKa = 10.45HH199 pKa = 7.73AGEE202 pKa = 4.32ADD204 pKa = 4.14EE205 pKa = 4.64ISGIEE210 pKa = 3.98IVDD213 pKa = 3.72EE214 pKa = 4.08KK215 pKa = 10.18TLKK218 pKa = 8.62ITHH221 pKa = 6.8HH222 pKa = 5.53EE223 pKa = 4.27TGPSMVTGGIWTYY236 pKa = 11.87AMPKK240 pKa = 9.8HH241 pKa = 6.24LFSEE245 pKa = 4.44IPVAEE250 pKa = 4.25MEE252 pKa = 4.19EE253 pKa = 4.25SDD255 pKa = 4.74LVRR258 pKa = 11.84EE259 pKa = 4.43NPIGMGPFKK268 pKa = 10.94VEE270 pKa = 4.23SIVPGEE276 pKa = 4.06SVVYY280 pKa = 10.43SKK282 pKa = 11.64NEE284 pKa = 4.04DD285 pKa = 3.39YY286 pKa = 11.05WNGEE290 pKa = 3.9PKK292 pKa = 10.34LDD294 pKa = 3.67EE295 pKa = 4.28VVLKK299 pKa = 10.31VVAPEE304 pKa = 4.02TVVNEE309 pKa = 4.22LEE311 pKa = 4.27TGGVDD316 pKa = 4.36LVNSFPTDD324 pKa = 3.5QFPDD328 pKa = 3.51NADD331 pKa = 3.44MSNVEE336 pKa = 4.3YY337 pKa = 11.02LGMIDD342 pKa = 4.35NAYY345 pKa = 9.41TYY347 pKa = 10.51IGFKK351 pKa = 10.12LGHH354 pKa = 6.73WDD356 pKa = 3.31ADD358 pKa = 3.52AGEE361 pKa = 4.47VVMDD365 pKa = 5.34PDD367 pKa = 4.8AKK369 pKa = 10.39MADD372 pKa = 3.53VNLRR376 pKa = 11.84RR377 pKa = 11.84AMWHH381 pKa = 6.36AVDD384 pKa = 3.66NDD386 pKa = 3.49AVGRR390 pKa = 11.84EE391 pKa = 4.44FYY393 pKa = 11.05NGLRR397 pKa = 11.84WNATSLIIPFFPDD410 pKa = 3.49YY411 pKa = 11.41YY412 pKa = 10.83DD413 pKa = 3.82DD414 pKa = 5.15TIDD417 pKa = 5.4APTFDD422 pKa = 4.39PDD424 pKa = 3.15EE425 pKa = 4.81ANRR428 pKa = 11.84LLDD431 pKa = 3.47EE432 pKa = 5.25AGYY435 pKa = 11.09EE436 pKa = 4.15DD437 pKa = 4.66VDD439 pKa = 3.75GDD441 pKa = 4.42GIRR444 pKa = 11.84EE445 pKa = 4.01NPEE448 pKa = 3.57GEE450 pKa = 3.87EE451 pKa = 3.85LVINFASMSGGDD463 pKa = 3.53TAEE466 pKa = 5.14PIANYY471 pKa = 9.89YY472 pKa = 8.09IQSWEE477 pKa = 3.93NVGLKK482 pKa = 10.25VEE484 pKa = 4.32LVDD487 pKa = 4.19GRR489 pKa = 11.84LMEE492 pKa = 4.65FNSFYY497 pKa = 11.34DD498 pKa = 3.27RR499 pKa = 11.84VEE501 pKa = 4.91ADD503 pKa = 4.8DD504 pKa = 4.81PDD506 pKa = 4.01IDD508 pKa = 4.01IYY510 pKa = 10.83QGAWGTGTDD519 pKa = 3.79VNPSGLYY526 pKa = 9.94GRR528 pKa = 11.84NASYY532 pKa = 10.93NYY534 pKa = 9.25PRR536 pKa = 11.84YY537 pKa = 10.01ASEE540 pKa = 4.02EE541 pKa = 3.88NDD543 pKa = 3.29RR544 pKa = 11.84LLAEE548 pKa = 4.88GNSEE552 pKa = 3.88AALDD556 pKa = 3.56VEE558 pKa = 4.73YY559 pKa = 10.64RR560 pKa = 11.84QEE562 pKa = 5.07VYY564 pKa = 10.78SEE566 pKa = 3.92WQQLMVEE573 pKa = 5.23DD574 pKa = 4.23IPVFPTLYY582 pKa = 9.07RR583 pKa = 11.84SVVIPVNEE591 pKa = 3.72RR592 pKa = 11.84VINYY596 pKa = 9.85SIAPDD601 pKa = 3.17KK602 pKa = 10.83YY603 pKa = 8.57IRR605 pKa = 11.84HH606 pKa = 6.66EE607 pKa = 3.79IAVTQEE613 pKa = 3.92EE614 pKa = 4.83PVVAEE619 pKa = 4.13

Molecular weight: 69.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0GWR6|A0A1I0GWR6_9BACI Non-homologous end joining protein Ku OS=Oceanobacillus limi OX=930131 GN=ku PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 7.99KK14 pKa = 9.24VHH16 pKa = 5.91GFRR19 pKa = 11.84QRR21 pKa = 11.84MSTKK25 pKa = 10.15NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3982

0

3982

1095241

27

1965

275.0

31.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.4 ± 0.043

0.618 ± 0.011

5.541 ± 0.035

7.852 ± 0.043

4.587 ± 0.038

6.63 ± 0.035

2.105 ± 0.019

8.119 ± 0.039

6.696 ± 0.042

9.489 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.779 ± 0.018

4.781 ± 0.029

3.44 ± 0.024

3.812 ± 0.03

3.96 ± 0.032

6.021 ± 0.026

5.483 ± 0.028

6.965 ± 0.032

1.031 ± 0.016

3.692 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski