Accumulibacter phosphatis (strain UW-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Accumulibacter; Candidatus Accumulibacter phosphatis

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4438 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7RNV6|C7RNV6_ACCPU Acetyl-CoA acetyltransferase OS=Accumulibacter phosphatis (strain UW-1) OX=522306 GN=CAP2UW1_2144 PE=3 SV=1
MM1 pKa = 8.02LIRR4 pKa = 11.84ITYY7 pKa = 8.54AFPTAASGIYY17 pKa = 10.04GSQEE21 pKa = 3.32LSGFVGLNAQQQAQAEE37 pKa = 4.41RR38 pKa = 11.84ALQTWDD44 pKa = 4.52DD45 pKa = 4.96LIAPDD50 pKa = 4.16MVRR53 pKa = 11.84TTATTSNIEE62 pKa = 4.29FGTSTTGVSYY72 pKa = 11.03AHH74 pKa = 7.38AYY76 pKa = 9.83FPTTGSVWFSRR87 pKa = 11.84SYY89 pKa = 11.41ADD91 pKa = 4.73LMSPQVGQHH100 pKa = 5.33SFMAYY105 pKa = 6.97VHH107 pKa = 6.84EE108 pKa = 5.13IGHH111 pKa = 6.6ALGLDD116 pKa = 3.23HH117 pKa = 6.94MGDD120 pKa = 4.13YY121 pKa = 10.66NGSGSWTPSSYY132 pKa = 10.82EE133 pKa = 3.66DD134 pKa = 3.22SGVYY138 pKa = 10.3SVMSYY143 pKa = 10.07FGPNMNSGSGQVAWADD159 pKa = 3.29WVGADD164 pKa = 3.8GKK166 pKa = 10.57LYY168 pKa = 10.7SPQTPMVNDD177 pKa = 3.11VMAIQAIYY185 pKa = 9.85GAEE188 pKa = 4.09TTTRR192 pKa = 11.84TGDD195 pKa = 3.24TVYY198 pKa = 10.97GFGSNVTGAMAPIFDD213 pKa = 4.2FTLNANPIVTIYY225 pKa = 10.91DD226 pKa = 3.4SAGIDD231 pKa = 3.6TLNLSGWNTACTINLAPGSYY251 pKa = 10.44SSGNSMTYY259 pKa = 10.5NIAIAYY265 pKa = 6.83TCDD268 pKa = 2.95IEE270 pKa = 4.4NAVGGSAGDD279 pKa = 3.62QITGNLLANRR289 pKa = 11.84LDD291 pKa = 3.91GGAGNDD297 pKa = 3.75TLSGGGGDD305 pKa = 4.04DD306 pKa = 3.62VLVAGAGDD314 pKa = 3.82DD315 pKa = 3.76VLDD318 pKa = 4.51GGEE321 pKa = 4.33GTDD324 pKa = 3.24SVVFSGTWGAYY335 pKa = 10.01SYY337 pKa = 10.87TCTNLYY343 pKa = 8.75ATTFAFTFYY352 pKa = 10.91GAATGTDD359 pKa = 3.43TLRR362 pKa = 11.84GIEE365 pKa = 4.22TFVFADD371 pKa = 3.39VTKK374 pKa = 10.62YY375 pKa = 10.88ASEE378 pKa = 4.08LSGAGPSPSLVSISSSAATQAEE400 pKa = 4.7GNAGQTLFSYY410 pKa = 10.31KK411 pKa = 10.21VSLSAASSSVQTVNWSLSGTGSSAADD437 pKa = 3.54PASDD441 pKa = 4.24FSGATSGSITFQVGEE456 pKa = 4.33TEE458 pKa = 4.06KK459 pKa = 10.4TIQIYY464 pKa = 10.78ARR466 pKa = 11.84GDD468 pKa = 3.46TSVEE472 pKa = 3.8ADD474 pKa = 3.16EE475 pKa = 5.44HH476 pKa = 5.57FTVTLSNPSAGLKK489 pKa = 9.56FASSSAQGTILNDD502 pKa = 3.64DD503 pKa = 3.96TSSGGDD509 pKa = 3.38DD510 pKa = 3.92FGSTTASAGNLAVNGGAVSGAIEE533 pKa = 4.0RR534 pKa = 11.84AGDD537 pKa = 3.52LDD539 pKa = 4.14LFRR542 pKa = 11.84VNLNAGVTYY551 pKa = 10.89VFDD554 pKa = 4.52LVATGSGVDD563 pKa = 4.19PYY565 pKa = 11.43LSLYY569 pKa = 8.98ATTATSLSAVASNDD583 pKa = 2.84NVRR586 pKa = 11.84AGTSSAQITYY596 pKa = 7.72TASSSATYY604 pKa = 10.77YY605 pKa = 11.28LLARR609 pKa = 11.84DD610 pKa = 3.67TWTGTGSYY618 pKa = 9.02TISAITFAGRR628 pKa = 11.84TITGDD633 pKa = 3.03EE634 pKa = 3.92AANRR638 pKa = 11.84LVGTLGDD645 pKa = 3.75DD646 pKa = 3.44VLYY649 pKa = 10.99GLGGADD655 pKa = 3.55VLSGDD660 pKa = 4.31GGDD663 pKa = 4.33DD664 pKa = 3.56RR665 pKa = 11.84LDD667 pKa = 3.89GGSGADD673 pKa = 3.84AMSGGNGNDD682 pKa = 2.79IYY684 pKa = 11.68VVDD687 pKa = 4.26NVADD691 pKa = 4.14SVSEE695 pKa = 3.93TSAAGGIDD703 pKa = 3.06RR704 pKa = 11.84VEE706 pKa = 4.3SSVSFTLGTNLEE718 pKa = 4.15NLVLTGNADD727 pKa = 3.59SNGTGNSQNNQLTGNSGNNILDD749 pKa = 3.85GKK751 pKa = 10.83AGLDD755 pKa = 3.54TFAGGLGNDD764 pKa = 3.63TYY766 pKa = 11.5LLDD769 pKa = 4.5MEE771 pKa = 5.6GEE773 pKa = 4.2LANVTEE779 pKa = 4.45NAGQGTDD786 pKa = 3.21TLTVVYY792 pKa = 10.37ANTSAVKK799 pKa = 10.5SIALTGNLANIEE811 pKa = 4.07NVLLSGAGLFNVLGNGLANALTGNASVNRR840 pKa = 11.84LEE842 pKa = 4.51GAAGNDD848 pKa = 3.57TLDD851 pKa = 3.9GQGGADD857 pKa = 3.62QLIGGQGDD865 pKa = 3.76DD866 pKa = 3.78VYY868 pKa = 11.48KK869 pKa = 10.57VDD871 pKa = 3.83QLGDD875 pKa = 3.71VLIEE879 pKa = 4.29NADD882 pKa = 3.77EE883 pKa = 4.92GSDD886 pKa = 3.61TVLVALSASGTSYY899 pKa = 11.57VLGNHH904 pKa = 6.85LEE906 pKa = 4.21NATVVAAVAINLTGNDD922 pKa = 3.84LANVLTGNALANRR935 pKa = 11.84LDD937 pKa = 3.84GGAGIDD943 pKa = 3.81TLIGGAGGDD952 pKa = 3.85TYY954 pKa = 11.71VVDD957 pKa = 3.5QVGDD961 pKa = 3.78VLVEE965 pKa = 4.11TSSLASEE972 pKa = 4.28IDD974 pKa = 3.43RR975 pKa = 11.84VEE977 pKa = 4.9SYY979 pKa = 11.58VDD981 pKa = 3.73WILGANLEE989 pKa = 4.03NLTLLGNGNLAGTGNALNNTLIGNAGANRR1018 pKa = 11.84LDD1020 pKa = 3.92GGQGADD1026 pKa = 3.49ILLGGAGDD1034 pKa = 3.91DD1035 pKa = 3.91RR1036 pKa = 11.84YY1037 pKa = 11.31VVDD1040 pKa = 3.81NLGDD1044 pKa = 3.59KK1045 pKa = 10.73VYY1047 pKa = 8.86EE1048 pKa = 4.45TTTSTSAYY1056 pKa = 9.56DD1057 pKa = 3.23AGGIDD1062 pKa = 4.11TVDD1065 pKa = 3.83SSINWTLGNFVEE1077 pKa = 4.63NLTLTGSGNLSGIGNALANTLRR1099 pKa = 11.84GNAGINVLDD1108 pKa = 4.5GKK1110 pKa = 10.89AGVDD1114 pKa = 3.49VLDD1117 pKa = 4.49GGEE1120 pKa = 4.15GSDD1123 pKa = 3.3IYY1125 pKa = 11.39LVGLASDD1132 pKa = 4.0HH1133 pKa = 6.4AAAEE1137 pKa = 4.38IADD1140 pKa = 4.02SGNGGSDD1147 pKa = 3.22EE1148 pKa = 4.16VRR1150 pKa = 11.84FAASSSGTLTLFAGDD1165 pKa = 3.53TGIEE1169 pKa = 3.89RR1170 pKa = 11.84VVIGSGLGEE1179 pKa = 4.1VAVSSGTLALDD1190 pKa = 3.43VDD1192 pKa = 4.49ASRR1195 pKa = 11.84VANALSVLGNAGANVLTGTAYY1216 pKa = 10.48ADD1218 pKa = 4.15RR1219 pKa = 11.84LDD1221 pKa = 4.24GGAGADD1227 pKa = 3.65RR1228 pKa = 11.84LIGGGGADD1236 pKa = 3.82TLIGGAGNDD1245 pKa = 3.56VYY1247 pKa = 11.66VVDD1250 pKa = 4.7SGDD1253 pKa = 4.12AIIEE1257 pKa = 4.23ASTLASEE1264 pKa = 4.49IDD1266 pKa = 3.54RR1267 pKa = 11.84VEE1269 pKa = 4.9SYY1271 pKa = 11.58VDD1273 pKa = 3.73WILGANLEE1281 pKa = 4.08NLTLLGTGNLAGTGNALNNTLTGNAGANRR1310 pKa = 11.84LDD1312 pKa = 3.92GGQGADD1318 pKa = 3.49ILLGGAGDD1326 pKa = 3.91DD1327 pKa = 3.91RR1328 pKa = 11.84YY1329 pKa = 11.31VVDD1332 pKa = 3.81NLGDD1336 pKa = 3.59KK1337 pKa = 10.73VYY1339 pKa = 8.86EE1340 pKa = 4.45TTTSTSQYY1348 pKa = 10.74DD1349 pKa = 3.26AGGTDD1354 pKa = 3.75TVDD1357 pKa = 4.54SSINWTLGNFVEE1369 pKa = 4.63NLTLTGSGNLSGIGNALANTLRR1391 pKa = 11.84GNAGINVLDD1400 pKa = 4.5GKK1402 pKa = 10.89AGVDD1406 pKa = 3.49VLDD1409 pKa = 4.49GGEE1412 pKa = 4.15GSDD1415 pKa = 3.3IYY1417 pKa = 11.39LVGLASDD1424 pKa = 4.0HH1425 pKa = 6.4AAAEE1429 pKa = 4.38IADD1432 pKa = 4.02SGNGGSDD1439 pKa = 3.24EE1440 pKa = 4.24VRR1442 pKa = 11.84FAANTSGTLTLFAGDD1457 pKa = 3.53TGIEE1461 pKa = 3.89RR1462 pKa = 11.84VVIGSGLGEE1471 pKa = 4.1VAVSSGTLALDD1482 pKa = 3.43VDD1484 pKa = 4.49ASRR1487 pKa = 11.84VANALSLLGNAGANVLTGTAYY1508 pKa = 10.65ADD1510 pKa = 3.91TLDD1513 pKa = 4.29GGAGADD1519 pKa = 3.8RR1520 pKa = 11.84LIGGDD1525 pKa = 3.58GNDD1528 pKa = 3.74RR1529 pKa = 11.84LIGGAGNDD1537 pKa = 3.92TLTGGAGADD1546 pKa = 3.34QFVLTQALSSTTNRR1560 pKa = 11.84DD1561 pKa = 2.87VLADD1565 pKa = 4.35FVSGTDD1571 pKa = 3.73TIQLSVSVFRR1581 pKa = 11.84AVTSRR1586 pKa = 11.84DD1587 pKa = 2.38IWNSSTRR1594 pKa = 11.84AIRR1597 pKa = 11.84GG1598 pKa = 3.36

Molecular weight: 160.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7RSN1|C7RSN1_ACCPU von Willebrand factor type A OS=Accumulibacter phosphatis (strain UW-1) OX=522306 GN=CAP2UW1_2647 PE=4 SV=1
MM1 pKa = 7.71ARR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 10.06KK6 pKa = 10.25PSRR9 pKa = 11.84LLLLQLLLPLPLLPLLLRR27 pKa = 11.84LPLLPLLLRR36 pKa = 11.84LLRR39 pKa = 11.84PRR41 pKa = 11.84MLRR44 pKa = 11.84SPPMPPNPPTPPWTRR59 pKa = 11.84PRR61 pKa = 11.84KK62 pKa = 9.9LPALLRR68 pKa = 11.84KK69 pKa = 9.28ALPMQPKK76 pKa = 8.11MQQ78 pKa = 4.02

Molecular weight: 9.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4438

0

4438

1504008

30

5854

338.9

36.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.498 ± 0.046

1.023 ± 0.013

5.49 ± 0.05

5.538 ± 0.04

3.614 ± 0.023

8.18 ± 0.065

2.173 ± 0.02

4.592 ± 0.023

2.98 ± 0.032

11.359 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.023

2.538 ± 0.027

5.038 ± 0.032

3.647 ± 0.028

7.551 ± 0.046

5.542 ± 0.029

4.951 ± 0.048

7.461 ± 0.039

1.426 ± 0.016

2.269 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski