Amino acid dipepetide frequency for Psychromonas sp. RZ22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.493AlaAla: 6.493 ± 0.115	0.974AlaCys: 0.974 ± 0.033	4.289AlaAsp: 4.289 ± 0.079	5.031AlaGlu: 5.031 ± 0.093	3.384AlaPhe: 3.384 ± 0.067	5.473AlaGly: 5.473 ± 0.114	1.55AlaHis: 1.55 ± 0.044	6.317AlaIle: 6.317 ± 0.086	5.126AlaLys: 5.126 ± 0.08	9.118AlaLeu: 9.118 ± 0.127	2.274AlaMet: 2.274 ± 0.053	3.514AlaAsn: 3.514 ± 0.062	2.447AlaPro: 2.447 ± 0.058	3.504AlaGln: 3.504 ± 0.071	2.916AlaArg: 2.916 ± 0.066	4.961AlaSer: 4.961 ± 0.083	4.535AlaThr: 4.535 ± 0.083	5.48AlaVal: 5.48 ± 0.091	0.874AlaTrp: 0.874 ± 0.035	2.386AlaTyr: 2.386 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.775CysAla: 0.775 ± 0.028	0.178CysCys: 0.178 ± 0.014	0.58CysAsp: 0.58 ± 0.028	0.648CysGlu: 0.648 ± 0.028	0.49CysPhe: 0.49 ± 0.024	0.834CysGly: 0.834 ± 0.032	0.336CysHis: 0.336 ± 0.018	0.729CysIle: 0.729 ± 0.027	0.543CysLys: 0.543 ± 0.025	1.054CysLeu: 1.054 ± 0.037	0.22CysMet: 0.22 ± 0.017	0.396CysAsn: 0.396 ± 0.023	0.428CysPro: 0.428 ± 0.025	0.45CysGln: 0.45 ± 0.021	0.392CysArg: 0.392 ± 0.023	0.756CysSer: 0.756 ± 0.032	0.506CysThr: 0.506 ± 0.021	0.653CysVal: 0.653 ± 0.031	0.121CysTrp: 0.121 ± 0.012	0.379CysTyr: 0.379 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.259AspAla: 4.259 ± 0.089	0.564AspCys: 0.564 ± 0.025	2.851AspAsp: 2.851 ± 0.073	3.47AspGlu: 3.47 ± 0.078	2.526AspPhe: 2.526 ± 0.053	3.422AspGly: 3.422 ± 0.115	1.129AspHis: 1.129 ± 0.039	4.395AspIle: 4.395 ± 0.076	3.738AspLys: 3.738 ± 0.068	5.579AspLeu: 5.579 ± 0.087	1.216AspMet: 1.216 ± 0.038	2.816AspAsn: 2.816 ± 0.057	1.985AspPro: 1.985 ± 0.052	2.099AspGln: 2.099 ± 0.05	1.911AspArg: 1.911 ± 0.048	3.252AspSer: 3.252 ± 0.078	2.704AspThr: 2.704 ± 0.077	3.58AspVal: 3.58 ± 0.075	0.752AspTrp: 0.752 ± 0.029	2.087AspTyr: 2.087 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	4.7GluAla: 4.7 ± 0.088	0.494GluCys: 0.494 ± 0.026	2.846GluAsp: 2.846 ± 0.064	3.472GluGlu: 3.472 ± 0.08	2.525GluPhe: 2.525 ± 0.057	3.391GluGly: 3.391 ± 0.079	1.529GluHis: 1.529 ± 0.051	4.245GluIle: 4.245 ± 0.074	4.353GluLys: 4.353 ± 0.078	6.826GluLeu: 6.826 ± 0.085	1.673GluMet: 1.673 ± 0.047	3.29GluAsn: 3.29 ± 0.06	1.648GluPro: 1.648 ± 0.048	4.011GluGln: 4.011 ± 0.077	2.606GluArg: 2.606 ± 0.064	3.739GluSer: 3.739 ± 0.075	3.068GluThr: 3.068 ± 0.062	4.164GluVal: 4.164 ± 0.076	0.631GluTrp: 0.631 ± 0.025	1.836GluTyr: 1.836 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.515PheAla: 3.515 ± 0.069	0.56PheCys: 0.56 ± 0.024	2.832PheAsp: 2.832 ± 0.061	2.536PheGlu: 2.536 ± 0.056	1.872PhePhe: 1.872 ± 0.056	2.746PheGly: 2.746 ± 0.063	0.858PheHis: 0.858 ± 0.031	3.631PheIle: 3.631 ± 0.084	2.52PheLys: 2.52 ± 0.055	3.865PheLeu: 3.865 ± 0.079	1.008PheMet: 1.008 ± 0.031	2.394PheAsn: 2.394 ± 0.052	1.471PhePro: 1.471 ± 0.042	1.277PheGln: 1.277 ± 0.036	1.312PheArg: 1.312 ± 0.041	3.717PheSer: 3.717 ± 0.065	2.716PheThr: 2.716 ± 0.059	2.737PheVal: 2.737 ± 0.063	0.475PheTrp: 0.475 ± 0.023	1.494PheTyr: 1.494 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	4.818GlyAla: 4.818 ± 0.1	0.842GlyCys: 0.842 ± 0.028	3.464GlyAsp: 3.464 ± 0.077	4.05GlyGlu: 4.05 ± 0.083	3.109GlyPhe: 3.109 ± 0.058	4.328GlyGly: 4.328 ± 0.095	1.412GlyHis: 1.412 ± 0.049	4.915GlyIle: 4.915 ± 0.072	4.129GlyLys: 4.129 ± 0.079	6.671GlyLeu: 6.671 ± 0.101	1.698GlyMet: 1.698 ± 0.05	2.587GlyAsn: 2.587 ± 0.061	1.473GlyPro: 1.473 ± 0.045	2.536GlyGln: 2.536 ± 0.061	2.531GlyArg: 2.531 ± 0.062	3.799GlySer: 3.799 ± 0.077	3.305GlyThr: 3.305 ± 0.091	4.624GlyVal: 4.624 ± 0.079	0.77GlyTrp: 0.77 ± 0.03	2.427GlyTyr: 2.427 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.457HisAla: 1.457 ± 0.044	0.368HisCys: 0.368 ± 0.019	1.058HisAsp: 1.058 ± 0.038	1.151HisGlu: 1.151 ± 0.044	1.26HisPhe: 1.26 ± 0.039	1.382HisGly: 1.382 ± 0.052	0.663HisHis: 0.663 ± 0.031	1.56HisIle: 1.56 ± 0.042	1.186HisLys: 1.186 ± 0.036	2.457HisLeu: 2.457 ± 0.057	0.416HisMet: 0.416 ± 0.022	1.012HisAsn: 1.012 ± 0.032	1.059HisPro: 1.059 ± 0.034	1.216HisGln: 1.216 ± 0.044	0.87HisArg: 0.87 ± 0.034	1.437HisSer: 1.437 ± 0.039	0.985HisThr: 0.985 ± 0.032	1.182HisVal: 1.182 ± 0.033	0.324HisTrp: 0.324 ± 0.02	0.88HisTyr: 0.88 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	6.996IleAla: 6.996 ± 0.09	0.812IleCys: 0.812 ± 0.034	4.792IleAsp: 4.792 ± 0.082	5.173IleGlu: 5.173 ± 0.085	2.834IlePhe: 2.834 ± 0.062	4.784IleGly: 4.784 ± 0.083	1.378IleHis: 1.378 ± 0.044	5.099IleIle: 5.099 ± 0.092	4.814IleLys: 4.814 ± 0.074	6.448IleLeu: 6.448 ± 0.101	1.411IleMet: 1.411 ± 0.039	4.013IleAsn: 4.013 ± 0.072	2.837IlePro: 2.837 ± 0.056	2.554IleGln: 2.554 ± 0.055	2.535IleArg: 2.535 ± 0.055	5.33IleSer: 5.33 ± 0.083	4.511IleThr: 4.511 ± 0.098	4.497IleVal: 4.497 ± 0.063	0.674IleTrp: 0.674 ± 0.029	2.112IleTyr: 2.112 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	5.059LysAla: 5.059 ± 0.084	0.394LysCys: 0.394 ± 0.022	3.098LysAsp: 3.098 ± 0.069	3.979LysGlu: 3.979 ± 0.077	1.866LysPhe: 1.866 ± 0.046	3.502LysGly: 3.502 ± 0.064	1.407LysHis: 1.407 ± 0.04	4.164LysIle: 4.164 ± 0.078	4.356LysLys: 4.356 ± 0.092	6.176LysLeu: 6.176 ± 0.098	1.677LysMet: 1.677 ± 0.04	3.258LysAsn: 3.258 ± 0.067	2.25LysPro: 2.25 ± 0.059	3.667LysGln: 3.667 ± 0.085	2.772LysArg: 2.772 ± 0.061	3.961LysSer: 3.961 ± 0.079	3.393LysThr: 3.393 ± 0.069	4.18LysVal: 4.18 ± 0.069	0.604LysTrp: 0.604 ± 0.025	1.636LysTyr: 1.636 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	9.23LeuAla: 9.23 ± 0.121	1.045LeuCys: 1.045 ± 0.035	5.628LeuAsp: 5.628 ± 0.081	5.551LeuGlu: 5.551 ± 0.087	4.874LeuPhe: 4.874 ± 0.097	6.505LeuGly: 6.505 ± 0.093	2.123LeuHis: 2.123 ± 0.059	7.796LeuIle: 7.796 ± 0.117	6.149LeuLys: 6.149 ± 0.089	11.817LeuLeu: 11.817 ± 0.18	2.595LeuMet: 2.595 ± 0.06	5.262LeuAsn: 5.262 ± 0.08	4.589LeuPro: 4.589 ± 0.082	4.502LeuGln: 4.502 ± 0.096	3.924LeuArg: 3.924 ± 0.07	8.214LeuSer: 8.214 ± 0.114	6.586LeuThr: 6.586 ± 0.098	6.537LeuVal: 6.537 ± 0.106	0.945LeuTrp: 0.945 ± 0.036	2.842LeuTyr: 2.842 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	2.078MetAla: 2.078 ± 0.047	0.179MetCys: 0.179 ± 0.013	1.165MetAsp: 1.165 ± 0.04	1.104MetGlu: 1.104 ± 0.039	0.901MetPhe: 0.901 ± 0.031	1.671MetGly: 1.671 ± 0.048	0.486MetHis: 0.486 ± 0.025	1.601MetIle: 1.601 ± 0.043	1.535MetLys: 1.535 ± 0.045	2.642MetLeu: 2.642 ± 0.06	0.674MetMet: 0.674 ± 0.031	1.131MetAsn: 1.131 ± 0.036	1.148MetPro: 1.148 ± 0.036	1.186MetGln: 1.186 ± 0.043	1.034MetArg: 1.034 ± 0.043	1.813MetSer: 1.813 ± 0.044	1.429MetThr: 1.429 ± 0.043	1.577MetVal: 1.577 ± 0.046	0.199MetTrp: 0.199 ± 0.015	0.463MetTyr: 0.463 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.646AsnAla: 3.646 ± 0.059	0.475AsnCys: 0.475 ± 0.022	2.687AsnAsp: 2.687 ± 0.066	2.938AsnGlu: 2.938 ± 0.06	1.837AsnPhe: 1.837 ± 0.048	2.914AsnGly: 2.914 ± 0.07	1.025AsnHis: 1.025 ± 0.04	3.899AsnIle: 3.899 ± 0.056	3.655AsnLys: 3.655 ± 0.075	4.457AsnLeu: 4.457 ± 0.069	1.048AsnMet: 1.048 ± 0.036	2.944AsnAsn: 2.944 ± 0.055	1.954AsnPro: 1.954 ± 0.048	2.091AsnGln: 2.091 ± 0.052	1.719AsnArg: 1.719 ± 0.048	3.112AsnSer: 3.112 ± 0.057	2.693AsnThr: 2.693 ± 0.061	2.82AsnVal: 2.82 ± 0.07	0.596AsnTrp: 0.596 ± 0.025	1.56AsnTyr: 1.56 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	2.766ProAla: 2.766 ± 0.061	0.316ProCys: 0.316 ± 0.018	2.028ProAsp: 2.028 ± 0.054	3.073ProGlu: 3.073 ± 0.064	1.714ProPhe: 1.714 ± 0.043	1.964ProGly: 1.964 ± 0.051	0.761ProHis: 0.761 ± 0.029	2.771ProIle: 2.771 ± 0.055	2.007ProLys: 2.007 ± 0.057	3.894ProLeu: 3.894 ± 0.062	0.861ProMet: 0.861 ± 0.032	1.617ProAsn: 1.617 ± 0.04	0.957ProPro: 0.957 ± 0.038	1.381ProGln: 1.381 ± 0.041	1.161ProArg: 1.161 ± 0.037	2.335ProSer: 2.335 ± 0.056	2.125ProThr: 2.125 ± 0.052	2.632ProVal: 2.632 ± 0.057	0.427ProTrp: 0.427 ± 0.023	1.205ProTyr: 1.205 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	4.038GlnAla: 4.038 ± 0.07	0.418GlnCys: 0.418 ± 0.023	1.952GlnAsp: 1.952 ± 0.046	2.464GlnGlu: 2.464 ± 0.056	1.773GlnPhe: 1.773 ± 0.048	2.771GlnGly: 2.771 ± 0.06	1.264GlnHis: 1.264 ± 0.041	2.747GlnIle: 2.747 ± 0.067	2.767GlnLys: 2.767 ± 0.068	5.644GlnLeu: 5.644 ± 0.107	0.985GlnMet: 0.985 ± 0.042	1.735GlnAsn: 1.735 ± 0.049	1.493GlnPro: 1.493 ± 0.043	3.583GlnGln: 3.583 ± 0.101	2.046GlnArg: 2.046 ± 0.055	2.77GlnSer: 2.77 ± 0.057	2.372GlnThr: 2.372 ± 0.056	3.143GlnVal: 3.143 ± 0.068	0.603GlnTrp: 0.603 ± 0.027	1.48GlnTyr: 1.48 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.67ArgAla: 2.67 ± 0.055	0.422ArgCys: 0.422 ± 0.021	2.047ArgAsp: 2.047 ± 0.058	2.402ArgGlu: 2.402 ± 0.06	1.981ArgPhe: 1.981 ± 0.052	2.161ArgGly: 2.161 ± 0.053	0.922ArgHis: 0.922 ± 0.034	2.856ArgIle: 2.856 ± 0.06	2.125ArgLys: 2.125 ± 0.053	4.411ArgLeu: 4.411 ± 0.078	0.892ArgMet: 0.892 ± 0.031	1.693ArgAsn: 1.693 ± 0.044	1.353ArgPro: 1.353 ± 0.046	1.692ArgGln: 1.692 ± 0.051	1.735ArgArg: 1.735 ± 0.054	2.354ArgSer: 2.354 ± 0.049	1.866ArgThr: 1.866 ± 0.053	2.591ArgVal: 2.591 ± 0.055	0.49ArgTrp: 0.49 ± 0.023	1.544ArgTyr: 1.544 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	5.133SerAla: 5.133 ± 0.078	0.643SerCys: 0.643 ± 0.031	3.782SerAsp: 3.782 ± 0.094	4.177SerGlu: 4.177 ± 0.076	3.207SerPhe: 3.207 ± 0.061	4.678SerGly: 4.678 ± 0.08	1.532SerHis: 1.532 ± 0.042	5.024SerIle: 5.024 ± 0.078	3.657SerLys: 3.657 ± 0.071	7.399SerLeu: 7.399 ± 0.111	1.575SerMet: 1.575 ± 0.042	3.073SerAsn: 3.073 ± 0.068	2.331SerPro: 2.331 ± 0.049	2.862SerGln: 2.862 ± 0.056	2.554SerArg: 2.554 ± 0.056	4.448SerSer: 4.448 ± 0.092	3.499SerThr: 3.499 ± 0.067	4.498SerVal: 4.498 ± 0.08	0.722SerTrp: 0.722 ± 0.031	2.069SerTyr: 2.069 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.422ThrAla: 4.422 ± 0.08	0.513ThrCys: 0.513 ± 0.025	3.053ThrAsp: 3.053 ± 0.074	3.485ThrGlu: 3.485 ± 0.074	2.319ThrPhe: 2.319 ± 0.057	4.059ThrGly: 4.059 ± 0.077	1.275ThrHis: 1.275 ± 0.035	3.955ThrIle: 3.955 ± 0.076	2.704ThrLys: 2.704 ± 0.06	6.626ThrLeu: 6.626 ± 0.093	1.135ThrMet: 1.135 ± 0.035	2.369ThrAsn: 2.369 ± 0.06	2.618ThrPro: 2.618 ± 0.06	2.468ThrGln: 2.468 ± 0.053	1.966ThrArg: 1.966 ± 0.054	3.392ThrSer: 3.392 ± 0.076	3.216ThrThr: 3.216 ± 0.075	3.695ThrVal: 3.695 ± 0.09	0.61ThrTrp: 0.61 ± 0.026	1.614ThrTyr: 1.614 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	5.584ValAla: 5.584 ± 0.084	0.697ValCys: 0.697 ± 0.031	4.033ValAsp: 4.033 ± 0.087	4.301ValGlu: 4.301 ± 0.083	2.858ValPhe: 2.858 ± 0.069	4.194ValGly: 4.194 ± 0.081	1.208ValHis: 1.208 ± 0.037	5.159ValIle: 5.159 ± 0.073	3.81ValLys: 3.81 ± 0.068	6.589ValLeu: 6.589 ± 0.101	1.691ValMet: 1.691 ± 0.042	3.264ValAsn: 3.264 ± 0.061	2.201ValPro: 2.201 ± 0.053	2.157ValGln: 2.157 ± 0.056	2.285ValArg: 2.285 ± 0.053	4.623ValSer: 4.623 ± 0.085	3.961ValThr: 3.961 ± 0.104	4.726ValVal: 4.726 ± 0.083	0.609ValTrp: 0.609 ± 0.03	1.86ValTyr: 1.86 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.769TrpAla: 0.769 ± 0.027	0.15TrpCys: 0.15 ± 0.014	0.536TrpAsp: 0.536 ± 0.022	0.484TrpGlu: 0.484 ± 0.021	0.551TrpPhe: 0.551 ± 0.024	0.716TrpGly: 0.716 ± 0.026	0.277TrpHis: 0.277 ± 0.015	0.716TrpIle: 0.716 ± 0.034	0.536TrpLys: 0.536 ± 0.027	1.495TrpLeu: 1.495 ± 0.046	0.283TrpMet: 0.283 ± 0.019	0.428TrpAsn: 0.428 ± 0.023	0.417TrpPro: 0.417 ± 0.023	0.73TrpGln: 0.73 ± 0.031	0.479TrpArg: 0.479 ± 0.024	0.715TrpSer: 0.715 ± 0.033	0.464TrpThr: 0.464 ± 0.021	0.764TrpVal: 0.764 ± 0.029	0.145TrpTrp: 0.145 ± 0.012	0.346TrpTyr: 0.346 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.239TyrAla: 2.239 ± 0.053	0.427TyrCys: 0.427 ± 0.025	1.611TyrAsp: 1.611 ± 0.05	1.56TyrGlu: 1.56 ± 0.044	1.654TyrPhe: 1.654 ± 0.044	1.991TyrGly: 1.991 ± 0.047	0.788TyrHis: 0.788 ± 0.033	2.024TyrIle: 2.024 ± 0.054	1.694TyrLys: 1.694 ± 0.05	3.547TyrLeu: 3.547 ± 0.066	0.682TyrMet: 0.682 ± 0.03	1.273TyrAsn: 1.273 ± 0.038	1.335TyrPro: 1.335 ± 0.041	2.081TyrGln: 2.081 ± 0.055	1.46TyrArg: 1.46 ± 0.038	2.132TyrSer: 2.132 ± 0.055	1.562TyrThr: 1.562 ± 0.045	1.728TyrVal: 1.728 ± 0.041	0.431TyrTrp: 0.431 ± 0.023	1.108TyrTyr: 1.108 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2647 proteins (880803 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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