Amino acid dipepetide frequency for Calothrix sp. NIES-3974

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.07AlaAla: 7.07 ± 0.096
0.844AlaCys: 0.844 ± 0.027
3.965AlaAsp: 3.965 ± 0.056
4.739AlaGlu: 4.739 ± 0.068
2.716AlaPhe: 2.716 ± 0.053
5.28AlaGly: 5.28 ± 0.076
1.186AlaHis: 1.186 ± 0.033
7.132AlaIle: 7.132 ± 0.084
3.971AlaLys: 3.971 ± 0.053
7.627AlaLeu: 7.627 ± 0.106
1.665AlaMet: 1.665 ± 0.039
3.794AlaAsn: 3.794 ± 0.069
2.731AlaPro: 2.731 ± 0.05
3.891AlaGln: 3.891 ± 0.054
3.596AlaArg: 3.596 ± 0.05
4.536AlaSer: 4.536 ± 0.066
4.587AlaThr: 4.587 ± 0.06
5.412AlaVal: 5.412 ± 0.06
1.049AlaTrp: 1.049 ± 0.029
2.255AlaTyr: 2.255 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.645CysAla: 0.645 ± 0.025
0.173CysCys: 0.173 ± 0.012
0.642CysAsp: 0.642 ± 0.02
0.511CysGlu: 0.511 ± 0.019
0.416CysPhe: 0.416 ± 0.019
0.841CysGly: 0.841 ± 0.025
0.31CysHis: 0.31 ± 0.013
0.607CysIle: 0.607 ± 0.023
0.306CysLys: 0.306 ± 0.015
1.121CysLeu: 1.121 ± 0.028
0.156CysMet: 0.156 ± 0.011
0.353CysAsn: 0.353 ± 0.016
0.523CysPro: 0.523 ± 0.021
0.685CysGln: 0.685 ± 0.023
0.54CysArg: 0.54 ± 0.022
0.571CysSer: 0.571 ± 0.02
0.453CysThr: 0.453 ± 0.019
0.656CysVal: 0.656 ± 0.024
0.153CysTrp: 0.153 ± 0.011
0.333CysTyr: 0.333 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.605AspAla: 3.605 ± 0.05
0.524AspCys: 0.524 ± 0.017
2.179AspAsp: 2.179 ± 0.05
2.797AspGlu: 2.797 ± 0.052
2.482AspPhe: 2.482 ± 0.046
3.515AspGly: 3.515 ± 0.051
0.692AspHis: 0.692 ± 0.022
3.692AspIle: 3.692 ± 0.058
2.006AspLys: 2.006 ± 0.047
5.484AspLeu: 5.484 ± 0.072
0.851AspMet: 0.851 ± 0.026
2.173AspAsn: 2.173 ± 0.043
2.117AspPro: 2.117 ± 0.043
1.592AspGln: 1.592 ± 0.031
3.08AspArg: 3.08 ± 0.048
2.649AspSer: 2.649 ± 0.046
2.62AspThr: 2.62 ± 0.045
3.106AspVal: 3.106 ± 0.06
0.863AspTrp: 0.863 ± 0.028
1.794AspTyr: 1.794 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
4.563GluAla: 4.563 ± 0.068
0.462GluCys: 0.462 ± 0.018
2.455GluAsp: 2.455 ± 0.047
3.614GluGlu: 3.614 ± 0.065
2.462GluPhe: 2.462 ± 0.043
3.0GluGly: 3.0 ± 0.047
0.947GluHis: 0.947 ± 0.025
5.186GluIle: 5.186 ± 0.065
3.44GluLys: 3.44 ± 0.054
6.597GluLeu: 6.597 ± 0.079
1.398GluMet: 1.398 ± 0.031
3.165GluAsn: 3.165 ± 0.042
2.214GluPro: 2.214 ± 0.046
3.237GluGln: 3.237 ± 0.054
3.26GluArg: 3.26 ± 0.052
3.174GluSer: 3.174 ± 0.051
3.432GluThr: 3.432 ± 0.051
4.026GluVal: 4.026 ± 0.059
0.779GluTrp: 0.779 ± 0.026
1.976GluTyr: 1.976 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
3.59PheAla: 3.59 ± 0.052
0.554PheCys: 0.554 ± 0.022
2.222PheAsp: 2.222 ± 0.042
1.853PheGlu: 1.853 ± 0.043
1.757PhePhe: 1.757 ± 0.036
2.993PheGly: 2.993 ± 0.051
0.875PheHis: 0.875 ± 0.027
2.533PheIle: 2.533 ± 0.044
1.291PheLys: 1.291 ± 0.034
4.152PheLeu: 4.152 ± 0.064
0.741PheMet: 0.741 ± 0.021
1.713PheAsn: 1.713 ± 0.039
2.047PhePro: 2.047 ± 0.037
1.926PheGln: 1.926 ± 0.039
1.994PheArg: 1.994 ± 0.039
2.779PheSer: 2.779 ± 0.042
2.433PheThr: 2.433 ± 0.045
2.563PheVal: 2.563 ± 0.051
0.753PheTrp: 0.753 ± 0.026
1.358PheTyr: 1.358 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
4.534GlyAla: 4.534 ± 0.07
0.836GlyCys: 0.836 ± 0.026
3.493GlyAsp: 3.493 ± 0.058
4.446GlyGlu: 4.446 ± 0.061
3.196GlyPhe: 3.196 ± 0.052
4.834GlyGly: 4.834 ± 0.086
1.202GlyHis: 1.202 ± 0.034
5.518GlyIle: 5.518 ± 0.062
4.167GlyLys: 4.167 ± 0.059
6.847GlyLeu: 6.847 ± 0.074
1.687GlyMet: 1.687 ± 0.033
3.42GlyAsn: 3.42 ± 0.08
1.126GlyPro: 1.126 ± 0.03
2.793GlyGln: 2.793 ± 0.047
3.369GlyArg: 3.369 ± 0.048
3.809GlySer: 3.809 ± 0.062
3.727GlyThr: 3.727 ± 0.058
5.01GlyVal: 5.01 ± 0.072
1.14GlyTrp: 1.14 ± 0.031
2.389GlyTyr: 2.389 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
1.188HisAla: 1.188 ± 0.03
0.229HisCys: 0.229 ± 0.014
0.858HisAsp: 0.858 ± 0.025
0.934HisGlu: 0.934 ± 0.025
0.751HisPhe: 0.751 ± 0.023
1.179HisGly: 1.179 ± 0.031
0.814HisHis: 0.814 ± 0.029
1.238HisIle: 1.238 ± 0.032
0.723HisLys: 0.723 ± 0.022
2.362HisLeu: 2.362 ± 0.045
0.255HisMet: 0.255 ± 0.013
0.812HisAsn: 0.812 ± 0.026
1.606HisPro: 1.606 ± 0.036
1.487HisGln: 1.487 ± 0.034
1.233HisArg: 1.233 ± 0.031
1.149HisSer: 1.149 ± 0.03
1.016HisThr: 1.016 ± 0.026
1.023HisVal: 1.023 ± 0.027
0.32HisTrp: 0.32 ± 0.015
0.66HisTyr: 0.66 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
7.607IleAla: 7.607 ± 0.079
0.865IleCys: 0.865 ± 0.025
3.807IleAsp: 3.807 ± 0.048
4.0IleGlu: 4.0 ± 0.059
2.963IlePhe: 2.963 ± 0.051
4.688IleGly: 4.688 ± 0.056
1.547IleHis: 1.547 ± 0.029
4.724IleIle: 4.724 ± 0.065
2.918IleLys: 2.918 ± 0.043
7.272IleLeu: 7.272 ± 0.09
1.062IleMet: 1.062 ± 0.026
3.458IleAsn: 3.458 ± 0.063
4.233IlePro: 4.233 ± 0.061
3.503IleGln: 3.503 ± 0.055
3.565IleArg: 3.565 ± 0.051
4.752IleSer: 4.752 ± 0.062
4.134IleThr: 4.134 ± 0.064
4.199IleVal: 4.199 ± 0.057
1.028IleTrp: 1.028 ± 0.031
2.234IleTyr: 2.234 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
3.253LysAla: 3.253 ± 0.056
0.328LysCys: 0.328 ± 0.018
1.743LysAsp: 1.743 ± 0.038
2.158LysGlu: 2.158 ± 0.038
1.842LysPhe: 1.842 ± 0.04
2.394LysGly: 2.394 ± 0.041
0.775LysHis: 0.775 ± 0.02
3.823LysIle: 3.823 ± 0.059
2.119LysLys: 2.119 ± 0.048
5.286LysLeu: 5.286 ± 0.063
1.052LysMet: 1.052 ± 0.026
2.177LysAsn: 2.177 ± 0.038
2.328LysPro: 2.328 ± 0.043
2.469LysGln: 2.469 ± 0.044
2.204LysArg: 2.204 ± 0.046
2.958LysSer: 2.958 ± 0.053
2.756LysThr: 2.756 ± 0.046
2.765LysVal: 2.765 ± 0.045
0.534LysTrp: 0.534 ± 0.019
1.749LysTyr: 1.749 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
8.992LeuAla: 8.992 ± 0.079
0.962LeuCys: 0.962 ± 0.024
5.006LeuAsp: 5.006 ± 0.067
6.929LeuGlu: 6.929 ± 0.094
3.999LeuPhe: 3.999 ± 0.061
7.517LeuGly: 7.517 ± 0.078
1.994LeuHis: 1.994 ± 0.04
7.147LeuIle: 7.147 ± 0.09
4.85LeuLys: 4.85 ± 0.067
10.44LeuLeu: 10.44 ± 0.131
2.097LeuMet: 2.097 ± 0.041
4.489LeuAsn: 4.489 ± 0.063
5.511LeuPro: 5.511 ± 0.065
5.961LeuGln: 5.961 ± 0.08
5.789LeuArg: 5.789 ± 0.068
6.883LeuSer: 6.883 ± 0.085
6.228LeuThr: 6.228 ± 0.068
6.858LeuVal: 6.858 ± 0.066
1.352LeuTrp: 1.352 ± 0.035
2.612LeuTyr: 2.612 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
1.708MetAla: 1.708 ± 0.035
0.133MetCys: 0.133 ± 0.01
0.851MetAsp: 0.851 ± 0.027
1.165MetGlu: 1.165 ± 0.029
0.637MetPhe: 0.637 ± 0.019
1.568MetGly: 1.568 ± 0.034
0.36MetHis: 0.36 ± 0.018
1.201MetIle: 1.201 ± 0.031
0.918MetLys: 0.918 ± 0.027
1.978MetLeu: 1.978 ± 0.037
0.564MetMet: 0.564 ± 0.02
0.888MetAsn: 0.888 ± 0.026
0.818MetPro: 0.818 ± 0.026
1.013MetGln: 1.013 ± 0.028
1.141MetArg: 1.141 ± 0.028
1.327MetSer: 1.327 ± 0.027
1.325MetThr: 1.325 ± 0.028
1.511MetVal: 1.511 ± 0.037
0.202MetTrp: 0.202 ± 0.012
0.466MetTyr: 0.466 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.053AsnAla: 3.053 ± 0.051
0.489AsnCys: 0.489 ± 0.019
1.605AsnAsp: 1.605 ± 0.041
1.623AsnGlu: 1.623 ± 0.033
2.078AsnPhe: 2.078 ± 0.047
2.859AsnGly: 2.859 ± 0.071
1.176AsnHis: 1.176 ± 0.031
3.163AsnIle: 3.163 ± 0.061
1.463AsnLys: 1.463 ± 0.036
5.929AsnLeu: 5.929 ± 0.079
0.745AsnMet: 0.745 ± 0.02
2.192AsnAsn: 2.192 ± 0.053
3.564AsnPro: 3.564 ± 0.061
3.294AsnGln: 3.294 ± 0.055
2.655AsnArg: 2.655 ± 0.048
3.195AsnSer: 3.195 ± 0.057
2.496AsnThr: 2.496 ± 0.06
2.22AsnVal: 2.22 ± 0.049
0.818AsnTrp: 0.818 ± 0.028
1.701AsnTyr: 1.701 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
3.07ProAla: 3.07 ± 0.05
0.34ProCys: 0.34 ± 0.016
2.901ProAsp: 2.901 ± 0.051
3.917ProGlu: 3.917 ± 0.062
1.74ProPhe: 1.74 ± 0.034
3.394ProGly: 3.394 ± 0.06
1.145ProHis: 1.145 ± 0.029
3.253ProIle: 3.253 ± 0.051
1.936ProLys: 1.936 ± 0.037
4.484ProLeu: 4.484 ± 0.058
0.812ProMet: 0.812 ± 0.021
2.347ProAsn: 2.347 ± 0.048
2.666ProPro: 2.666 ± 0.074
3.031ProGln: 3.031 ± 0.054
2.043ProArg: 2.043 ± 0.038
3.09ProSer: 3.09 ± 0.051
3.077ProThr: 3.077 ± 0.056
3.572ProVal: 3.572 ± 0.054
0.63ProTrp: 0.63 ± 0.023
1.326ProTyr: 1.326 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
4.667GlnAla: 4.667 ± 0.067
0.322GlnCys: 0.322 ± 0.017
2.107GlnAsp: 2.107 ± 0.043
3.616GlnGlu: 3.616 ± 0.056
1.78GlnPhe: 1.78 ± 0.034
3.309GlnGly: 3.309 ± 0.051
0.907GlnHis: 0.907 ± 0.025
4.276GlnIle: 4.276 ± 0.058
2.797GlnLys: 2.797 ± 0.05
5.773GlnLeu: 5.773 ± 0.076
1.092GlnMet: 1.092 ± 0.03
2.455GlnAsn: 2.455 ± 0.043
2.891GlnPro: 2.891 ± 0.058
3.899GlnGln: 3.899 ± 0.087
3.187GlnArg: 3.187 ± 0.056
2.941GlnSer: 2.941 ± 0.05
3.43GlnThr: 3.43 ± 0.056
4.167GlnVal: 4.167 ± 0.058
0.592GlnTrp: 0.592 ± 0.022
1.218GlnTyr: 1.218 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
3.174ArgAla: 3.174 ± 0.054
0.534ArgCys: 0.534 ± 0.02
2.979ArgAsp: 2.979 ± 0.051
3.641ArgGlu: 3.641 ± 0.052
2.286ArgPhe: 2.286 ± 0.034
3.304ArgGly: 3.304 ± 0.051
1.105ArgHis: 1.105 ± 0.029
3.781ArgIle: 3.781 ± 0.046
2.289ArgLys: 2.289 ± 0.041
5.736ArgLeu: 5.736 ± 0.065
1.17ArgMet: 1.17 ± 0.031
2.407ArgAsn: 2.407 ± 0.043
2.131ArgPro: 2.131 ± 0.042
3.429ArgGln: 3.429 ± 0.061
3.277ArgArg: 3.277 ± 0.066
3.085ArgSer: 3.085 ± 0.05
2.614ArgThr: 2.614 ± 0.042
3.724ArgVal: 3.724 ± 0.05
0.798ArgTrp: 0.798 ± 0.022
1.921ArgTyr: 1.921 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
4.316SerAla: 4.316 ± 0.06
0.593SerCys: 0.593 ± 0.023
2.832SerAsp: 2.832 ± 0.049
3.325SerGlu: 3.325 ± 0.049
2.358SerPhe: 2.358 ± 0.043
4.506SerGly: 4.506 ± 0.064
1.532SerHis: 1.532 ± 0.035
3.811SerIle: 3.811 ± 0.049
2.322SerLys: 2.322 ± 0.046
6.963SerLeu: 6.963 ± 0.083
1.226SerMet: 1.226 ± 0.031
2.605SerAsn: 2.605 ± 0.055
3.632SerPro: 3.632 ± 0.052
4.073SerGln: 4.073 ± 0.053
3.314SerArg: 3.314 ± 0.049
4.19SerSer: 4.19 ± 0.056
3.471SerThr: 3.471 ± 0.053
3.87SerVal: 3.87 ± 0.051
0.898SerTrp: 0.898 ± 0.027
1.835SerTyr: 1.835 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
4.716ThrAla: 4.716 ± 0.06
0.536ThrCys: 0.536 ± 0.02
2.694ThrAsp: 2.694 ± 0.047
3.127ThrGlu: 3.127 ± 0.042
1.973ThrPhe: 1.973 ± 0.039
4.528ThrGly: 4.528 ± 0.077
1.124ThrHis: 1.124 ± 0.03
3.961ThrIle: 3.961 ± 0.056
2.283ThrLys: 2.283 ± 0.043
5.763ThrLeu: 5.763 ± 0.067
0.866ThrMet: 0.866 ± 0.027
2.738ThrAsn: 2.738 ± 0.054
3.914ThrPro: 3.914 ± 0.07
3.019ThrGln: 3.019 ± 0.052
2.657ThrArg: 2.657 ± 0.044
3.701ThrSer: 3.701 ± 0.055
3.662ThrThr: 3.662 ± 0.065
3.95ThrVal: 3.95 ± 0.054
0.798ThrTrp: 0.798 ± 0.025
1.634ThrTyr: 1.634 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
5.535ValAla: 5.535 ± 0.069
0.711ValCys: 0.711 ± 0.026
3.441ValAsp: 3.441 ± 0.053
4.376ValGlu: 4.376 ± 0.064
2.674ValPhe: 2.674 ± 0.042
4.618ValGly: 4.618 ± 0.063
1.015ValHis: 1.015 ± 0.026
4.788ValIle: 4.788 ± 0.057
3.166ValLys: 3.166 ± 0.053
6.093ValLeu: 6.093 ± 0.073
1.524ValMet: 1.524 ± 0.032
3.333ValAsn: 3.333 ± 0.064
2.764ValPro: 2.764 ± 0.047
2.714ValGln: 2.714 ± 0.039
3.431ValArg: 3.431 ± 0.046
4.25ValSer: 4.25 ± 0.057
3.95ValThr: 3.95 ± 0.062
4.936ValVal: 4.936 ± 0.068
0.828ValTrp: 0.828 ± 0.025
1.934ValTyr: 1.934 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
0.689TrpAla: 0.689 ± 0.02
0.159TrpCys: 0.159 ± 0.01
0.67TrpAsp: 0.67 ± 0.02
1.013TrpGlu: 1.013 ± 0.027
0.621TrpPhe: 0.621 ± 0.024
1.03TrpGly: 1.03 ± 0.027
0.349TrpHis: 0.349 ± 0.018
0.868TrpIle: 0.868 ± 0.027
0.593TrpLys: 0.593 ± 0.022
1.951TrpLeu: 1.951 ± 0.045
0.331TrpMet: 0.331 ± 0.016
0.579TrpAsn: 0.579 ± 0.024
0.204TrpPro: 0.204 ± 0.012
1.255TrpGln: 1.255 ± 0.029
0.92TrpArg: 0.92 ± 0.026
0.873TrpSer: 0.873 ± 0.027
0.563TrpThr: 0.563 ± 0.021
0.928TrpVal: 0.928 ± 0.025
0.244TrpTrp: 0.244 ± 0.016
0.45TrpTyr: 0.45 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.072TyrAla: 2.072 ± 0.039
0.399TyrCys: 0.399 ± 0.017
1.368TyrAsp: 1.368 ± 0.032
1.601TyrGlu: 1.601 ± 0.036
1.393TyrPhe: 1.393 ± 0.032
2.186TyrGly: 2.186 ± 0.042
0.782TyrHis: 0.782 ± 0.024
1.861TyrIle: 1.861 ± 0.035
1.154TyrLys: 1.154 ± 0.03
3.758TyrLeu: 3.758 ± 0.058
0.481TyrMet: 0.481 ± 0.018
1.22TyrAsn: 1.22 ± 0.033
1.754TyrPro: 1.754 ± 0.036
2.158TyrGln: 2.158 ± 0.047
2.132TyrArg: 2.132 ± 0.043
1.761TyrSer: 1.761 ± 0.038
1.671TyrThr: 1.671 ± 0.035
1.584TyrVal: 1.584 ± 0.037
0.51TyrTrp: 0.51 ± 0.02
1.145TyrTyr: 1.145 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4751 proteins (1469257 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski