Amino acid dipepetide frequency for Enterococcus phage phiEf11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.249AlaAla: 3.249 ± 0.637
0.453AlaCys: 0.453 ± 0.188
4.005AlaAsp: 4.005 ± 0.535
4.609AlaGlu: 4.609 ± 0.701
1.964AlaPhe: 1.964 ± 0.353
4.231AlaGly: 4.231 ± 0.792
0.756AlaHis: 0.756 ± 0.267
5.969AlaIle: 5.969 ± 0.776
5.591AlaLys: 5.591 ± 0.595
4.987AlaLeu: 4.987 ± 0.75
1.813AlaMet: 1.813 ± 0.519
4.231AlaAsn: 4.231 ± 0.523
1.209AlaPro: 1.209 ± 0.297
2.342AlaGln: 2.342 ± 0.357
2.493AlaArg: 2.493 ± 0.414
3.4AlaSer: 3.4 ± 0.627
4.836AlaThr: 4.836 ± 0.715
4.156AlaVal: 4.156 ± 0.642
0.831AlaTrp: 0.831 ± 0.219
1.813AlaTyr: 1.813 ± 0.428
0.0AlaXaa: 0.0 ± 0.0
Cys
0.227CysAla: 0.227 ± 0.118
0.227CysCys: 0.227 ± 0.138
0.227CysAsp: 0.227 ± 0.142
0.604CysGlu: 0.604 ± 0.226
0.151CysPhe: 0.151 ± 0.103
0.453CysGly: 0.453 ± 0.191
0.151CysHis: 0.151 ± 0.111
0.302CysIle: 0.302 ± 0.182
0.604CysLys: 0.604 ± 0.215
0.453CysLeu: 0.453 ± 0.174
0.151CysMet: 0.151 ± 0.105
0.378CysAsn: 0.378 ± 0.156
0.076CysPro: 0.076 ± 0.092
0.151CysGln: 0.151 ± 0.106
0.302CysArg: 0.302 ± 0.138
0.453CysSer: 0.453 ± 0.173
0.302CysThr: 0.302 ± 0.157
0.151CysVal: 0.151 ± 0.101
0.0CysTrp: 0.0 ± 0.0
0.378CysTyr: 0.378 ± 0.173
0.0CysXaa: 0.0 ± 0.0
Asp
3.627AspAla: 3.627 ± 0.41
0.302AspCys: 0.302 ± 0.149
3.476AspAsp: 3.476 ± 0.56
4.836AspGlu: 4.836 ± 0.747
2.947AspPhe: 2.947 ± 0.442
2.871AspGly: 2.871 ± 0.433
0.756AspHis: 0.756 ± 0.268
4.458AspIle: 4.458 ± 0.564
5.062AspLys: 5.062 ± 0.805
4.307AspLeu: 4.307 ± 0.62
1.133AspMet: 1.133 ± 0.286
3.627AspAsn: 3.627 ± 0.513
1.738AspPro: 1.738 ± 0.298
1.813AspGln: 1.813 ± 0.388
1.813AspArg: 1.813 ± 0.431
4.08AspSer: 4.08 ± 0.692
3.325AspThr: 3.325 ± 0.447
4.156AspVal: 4.156 ± 0.514
0.68AspTrp: 0.68 ± 0.265
3.476AspTyr: 3.476 ± 0.557
0.0AspXaa: 0.0 ± 0.0
Glu
5.365GluAla: 5.365 ± 0.663
0.302GluCys: 0.302 ± 0.153
3.173GluAsp: 3.173 ± 0.534
5.591GluGlu: 5.591 ± 0.7
3.4GluPhe: 3.4 ± 0.5
3.476GluGly: 3.476 ± 0.519
1.133GluHis: 1.133 ± 0.27
5.893GluIle: 5.893 ± 0.652
8.765GluLys: 8.765 ± 1.158
7.329GluLeu: 7.329 ± 0.758
2.191GluMet: 2.191 ± 0.385
4.533GluAsn: 4.533 ± 0.527
2.04GluPro: 2.04 ± 0.422
3.325GluGln: 3.325 ± 0.495
3.627GluArg: 3.627 ± 0.435
3.929GluSer: 3.929 ± 0.422
4.307GluThr: 4.307 ± 0.554
5.138GluVal: 5.138 ± 0.704
0.68GluTrp: 0.68 ± 0.213
2.871GluTyr: 2.871 ± 0.477
0.0GluXaa: 0.0 ± 0.0
Phe
2.342PheAla: 2.342 ± 0.435
0.227PheCys: 0.227 ± 0.141
2.947PheAsp: 2.947 ± 0.544
3.4PheGlu: 3.4 ± 0.509
1.889PhePhe: 1.889 ± 0.534
2.191PheGly: 2.191 ± 0.378
0.453PheHis: 0.453 ± 0.155
3.098PheIle: 3.098 ± 0.572
3.173PheLys: 3.173 ± 0.531
2.72PheLeu: 2.72 ± 0.514
0.982PheMet: 0.982 ± 0.278
2.342PheAsn: 2.342 ± 0.444
1.511PhePro: 1.511 ± 0.371
1.436PheGln: 1.436 ± 0.382
1.436PheArg: 1.436 ± 0.276
3.022PheSer: 3.022 ± 0.391
2.418PheThr: 2.418 ± 0.454
2.418PheVal: 2.418 ± 0.447
0.529PheTrp: 0.529 ± 0.213
1.284PheTyr: 1.284 ± 0.319
0.0PheXaa: 0.0 ± 0.0
Gly
3.476GlyAla: 3.476 ± 0.69
0.227GlyCys: 0.227 ± 0.129
3.173GlyAsp: 3.173 ± 0.543
3.702GlyGlu: 3.702 ± 0.509
1.662GlyPhe: 1.662 ± 0.395
4.156GlyGly: 4.156 ± 1.456
1.058GlyHis: 1.058 ± 0.313
4.685GlyIle: 4.685 ± 0.673
5.667GlyLys: 5.667 ± 0.673
5.138GlyLeu: 5.138 ± 0.694
1.209GlyMet: 1.209 ± 0.263
3.627GlyAsn: 3.627 ± 0.471
0.831GlyPro: 0.831 ± 0.239
2.493GlyGln: 2.493 ± 0.483
1.738GlyArg: 1.738 ± 0.372
4.156GlySer: 4.156 ± 0.726
3.476GlyThr: 3.476 ± 0.405
4.458GlyVal: 4.458 ± 0.915
0.68GlyTrp: 0.68 ± 0.186
3.173GlyTyr: 3.173 ± 0.529
0.0GlyXaa: 0.0 ± 0.0
His
0.378HisAla: 0.378 ± 0.145
0.151HisCys: 0.151 ± 0.106
0.604HisAsp: 0.604 ± 0.252
0.907HisGlu: 0.907 ± 0.223
0.529HisPhe: 0.529 ± 0.232
0.756HisGly: 0.756 ± 0.239
0.604HisHis: 0.604 ± 0.188
0.907HisIle: 0.907 ± 0.289
1.058HisLys: 1.058 ± 0.364
1.133HisLeu: 1.133 ± 0.272
0.151HisMet: 0.151 ± 0.104
0.529HisAsn: 0.529 ± 0.178
0.302HisPro: 0.302 ± 0.164
0.604HisGln: 0.604 ± 0.206
0.151HisArg: 0.151 ± 0.1
1.133HisSer: 1.133 ± 0.437
1.058HisThr: 1.058 ± 0.386
1.36HisVal: 1.36 ± 0.328
0.378HisTrp: 0.378 ± 0.157
0.907HisTyr: 0.907 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
4.307IleAla: 4.307 ± 0.694
0.529IleCys: 0.529 ± 0.191
4.307IleAsp: 4.307 ± 0.644
5.818IleGlu: 5.818 ± 0.634
2.72IlePhe: 2.72 ± 0.587
3.929IleGly: 3.929 ± 0.525
1.058IleHis: 1.058 ± 0.352
3.476IleIle: 3.476 ± 0.523
6.725IleLys: 6.725 ± 0.634
5.062IleLeu: 5.062 ± 0.661
1.058IleMet: 1.058 ± 0.298
5.365IleAsn: 5.365 ± 0.691
2.947IlePro: 2.947 ± 0.496
3.249IleGln: 3.249 ± 0.419
2.645IleArg: 2.645 ± 0.557
5.516IleSer: 5.516 ± 0.652
4.307IleThr: 4.307 ± 0.595
3.929IleVal: 3.929 ± 0.45
0.604IleTrp: 0.604 ± 0.207
3.249IleTyr: 3.249 ± 0.623
0.0IleXaa: 0.0 ± 0.0
Lys
6.12LysAla: 6.12 ± 0.702
0.756LysCys: 0.756 ± 0.223
4.307LysAsp: 4.307 ± 0.62
8.765LysGlu: 8.765 ± 1.019
2.72LysPhe: 2.72 ± 0.438
4.987LysGly: 4.987 ± 0.775
1.133LysHis: 1.133 ± 0.35
6.12LysIle: 6.12 ± 0.852
9.369LysLys: 9.369 ± 0.921
7.48LysLeu: 7.48 ± 0.91
3.098LysMet: 3.098 ± 0.507
4.836LysAsn: 4.836 ± 0.577
2.267LysPro: 2.267 ± 0.364
4.458LysGln: 4.458 ± 0.65
3.853LysArg: 3.853 ± 0.569
4.609LysSer: 4.609 ± 0.507
5.667LysThr: 5.667 ± 0.678
5.818LysVal: 5.818 ± 0.691
1.058LysTrp: 1.058 ± 0.27
3.929LysTyr: 3.929 ± 0.526
0.0LysXaa: 0.0 ± 0.0
Leu
5.516LeuAla: 5.516 ± 0.475
0.529LeuCys: 0.529 ± 0.171
5.062LeuAsp: 5.062 ± 0.51
5.591LeuGlu: 5.591 ± 0.635
3.4LeuPhe: 3.4 ± 0.591
5.213LeuGly: 5.213 ± 0.563
0.68LeuHis: 0.68 ± 0.221
5.289LeuIle: 5.289 ± 0.714
8.538LeuLys: 8.538 ± 0.701
6.951LeuLeu: 6.951 ± 1.091
2.267LeuMet: 2.267 ± 0.566
5.516LeuAsn: 5.516 ± 0.69
2.871LeuPro: 2.871 ± 0.561
2.418LeuGln: 2.418 ± 0.361
3.022LeuArg: 3.022 ± 0.414
7.178LeuSer: 7.178 ± 0.7
5.44LeuThr: 5.44 ± 0.577
4.307LeuVal: 4.307 ± 0.572
0.982LeuTrp: 0.982 ± 0.225
2.04LeuTyr: 2.04 ± 0.392
0.0LeuXaa: 0.0 ± 0.0
Met
1.813MetAla: 1.813 ± 0.316
0.151MetCys: 0.151 ± 0.119
1.436MetAsp: 1.436 ± 0.299
1.436MetGlu: 1.436 ± 0.285
0.907MetPhe: 0.907 ± 0.29
0.907MetGly: 0.907 ± 0.281
0.453MetHis: 0.453 ± 0.187
1.964MetIle: 1.964 ± 0.378
2.72MetLys: 2.72 ± 0.536
1.964MetLeu: 1.964 ± 0.37
0.378MetMet: 0.378 ± 0.161
2.116MetAsn: 2.116 ± 0.339
1.209MetPro: 1.209 ± 0.257
1.209MetGln: 1.209 ± 0.347
1.209MetArg: 1.209 ± 0.27
1.587MetSer: 1.587 ± 0.295
0.982MetThr: 0.982 ± 0.244
1.284MetVal: 1.284 ± 0.298
0.227MetTrp: 0.227 ± 0.119
0.831MetTyr: 0.831 ± 0.298
0.0MetXaa: 0.0 ± 0.0
Asn
3.702AsnAla: 3.702 ± 0.618
0.227AsnCys: 0.227 ± 0.122
4.08AsnAsp: 4.08 ± 0.735
4.836AsnGlu: 4.836 ± 0.718
2.267AsnPhe: 2.267 ± 0.539
4.533AsnGly: 4.533 ± 0.547
0.756AsnHis: 0.756 ± 0.364
4.307AsnIle: 4.307 ± 0.592
5.969AsnLys: 5.969 ± 0.547
4.685AsnLeu: 4.685 ± 0.477
1.36AsnMet: 1.36 ± 0.292
4.005AsnAsn: 4.005 ± 0.61
2.569AsnPro: 2.569 ± 0.448
3.627AsnGln: 3.627 ± 0.636
2.645AsnArg: 2.645 ± 0.468
3.853AsnSer: 3.853 ± 0.658
3.4AsnThr: 3.4 ± 0.478
4.231AsnVal: 4.231 ± 0.573
0.604AsnTrp: 0.604 ± 0.203
1.662AsnTyr: 1.662 ± 0.386
0.0AsnXaa: 0.0 ± 0.0
Pro
1.813ProAla: 1.813 ± 0.314
0.227ProCys: 0.227 ± 0.135
2.04ProAsp: 2.04 ± 0.409
2.569ProGlu: 2.569 ± 0.47
1.36ProPhe: 1.36 ± 0.317
1.133ProGly: 1.133 ± 0.313
0.302ProHis: 0.302 ± 0.142
1.738ProIle: 1.738 ± 0.311
2.645ProLys: 2.645 ± 0.472
2.191ProLeu: 2.191 ± 0.385
0.68ProMet: 0.68 ± 0.209
2.493ProAsn: 2.493 ± 0.425
1.058ProPro: 1.058 ± 0.247
1.284ProGln: 1.284 ± 0.346
0.907ProArg: 0.907 ± 0.25
2.342ProSer: 2.342 ± 0.387
2.116ProThr: 2.116 ± 0.418
1.964ProVal: 1.964 ± 0.404
0.076ProTrp: 0.076 ± 0.077
1.36ProTyr: 1.36 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
3.627GlnAla: 3.627 ± 0.5
0.151GlnCys: 0.151 ± 0.098
1.813GlnAsp: 1.813 ± 0.443
3.627GlnGlu: 3.627 ± 0.605
1.058GlnPhe: 1.058 ± 0.262
1.964GlnGly: 1.964 ± 0.439
0.453GlnHis: 0.453 ± 0.173
2.569GlnIle: 2.569 ± 0.341
2.72GlnLys: 2.72 ± 0.528
4.156GlnLeu: 4.156 ± 0.522
1.058GlnMet: 1.058 ± 0.283
2.569GlnAsn: 2.569 ± 0.457
0.831GlnPro: 0.831 ± 0.285
2.645GlnGln: 2.645 ± 0.534
1.813GlnArg: 1.813 ± 0.385
2.796GlnSer: 2.796 ± 0.638
2.72GlnThr: 2.72 ± 0.472
2.116GlnVal: 2.116 ± 0.286
0.529GlnTrp: 0.529 ± 0.185
1.511GlnTyr: 1.511 ± 0.442
0.0GlnXaa: 0.0 ± 0.0
Arg
1.964ArgAla: 1.964 ± 0.34
0.453ArgCys: 0.453 ± 0.175
2.04ArgAsp: 2.04 ± 0.324
2.569ArgGlu: 2.569 ± 0.363
1.738ArgPhe: 1.738 ± 0.471
1.813ArgGly: 1.813 ± 0.32
0.453ArgHis: 0.453 ± 0.143
2.947ArgIle: 2.947 ± 0.508
4.08ArgLys: 4.08 ± 0.606
3.551ArgLeu: 3.551 ± 0.589
0.982ArgMet: 0.982 ± 0.259
2.418ArgAsn: 2.418 ± 0.413
0.982ArgPro: 0.982 ± 0.338
1.209ArgGln: 1.209 ± 0.396
1.36ArgArg: 1.36 ± 0.298
1.511ArgSer: 1.511 ± 0.352
2.191ArgThr: 2.191 ± 0.383
2.871ArgVal: 2.871 ± 0.444
0.076ArgTrp: 0.076 ± 0.077
1.133ArgTyr: 1.133 ± 0.278
0.0ArgXaa: 0.0 ± 0.0
Ser
3.476SerAla: 3.476 ± 0.6
0.151SerCys: 0.151 ± 0.114
3.551SerAsp: 3.551 ± 0.565
5.591SerGlu: 5.591 ± 0.603
2.871SerPhe: 2.871 ± 0.366
4.307SerGly: 4.307 ± 0.544
1.36SerHis: 1.36 ± 0.523
4.685SerIle: 4.685 ± 0.787
4.911SerLys: 4.911 ± 0.61
5.516SerLeu: 5.516 ± 0.761
2.191SerMet: 2.191 ± 0.455
4.458SerAsn: 4.458 ± 0.565
2.267SerPro: 2.267 ± 0.357
2.04SerGln: 2.04 ± 0.533
1.964SerArg: 1.964 ± 0.324
4.08SerSer: 4.08 ± 0.69
5.365SerThr: 5.365 ± 0.879
4.08SerVal: 4.08 ± 0.563
1.284SerTrp: 1.284 ± 0.274
2.569SerTyr: 2.569 ± 0.467
0.0SerXaa: 0.0 ± 0.0
Thr
5.138ThrAla: 5.138 ± 0.913
0.227ThrCys: 0.227 ± 0.123
4.533ThrAsp: 4.533 ± 0.723
4.609ThrGlu: 4.609 ± 0.617
2.871ThrPhe: 2.871 ± 0.532
4.836ThrGly: 4.836 ± 0.719
0.907ThrHis: 0.907 ± 0.388
4.458ThrIle: 4.458 ± 0.466
4.609ThrLys: 4.609 ± 0.651
5.516ThrLeu: 5.516 ± 0.58
1.662ThrMet: 1.662 ± 0.303
3.4ThrAsn: 3.4 ± 0.487
1.587ThrPro: 1.587 ± 0.513
1.964ThrGln: 1.964 ± 0.416
1.662ThrArg: 1.662 ± 0.315
4.458ThrSer: 4.458 ± 0.73
3.702ThrThr: 3.702 ± 0.593
4.08ThrVal: 4.08 ± 0.592
0.604ThrTrp: 0.604 ± 0.205
2.418ThrTyr: 2.418 ± 0.496
0.0ThrXaa: 0.0 ± 0.0
Val
3.702ValAla: 3.702 ± 0.817
0.227ValCys: 0.227 ± 0.146
4.836ValAsp: 4.836 ± 0.617
4.156ValGlu: 4.156 ± 0.63
2.72ValPhe: 2.72 ± 0.408
3.4ValGly: 3.4 ± 0.567
0.529ValHis: 0.529 ± 0.206
4.911ValIle: 4.911 ± 0.585
5.516ValLys: 5.516 ± 0.648
5.44ValLeu: 5.44 ± 0.758
1.436ValMet: 1.436 ± 0.34
4.005ValAsn: 4.005 ± 0.659
2.191ValPro: 2.191 ± 0.38
1.738ValGln: 1.738 ± 0.393
2.04ValArg: 2.04 ± 0.35
4.76ValSer: 4.76 ± 0.537
4.458ValThr: 4.458 ± 0.705
3.929ValVal: 3.929 ± 0.598
0.529ValTrp: 0.529 ± 0.174
2.645ValTyr: 2.645 ± 0.444
0.0ValXaa: 0.0 ± 0.0
Trp
0.982TrpAla: 0.982 ± 0.229
0.076TrpCys: 0.076 ± 0.073
0.529TrpAsp: 0.529 ± 0.175
0.831TrpGlu: 0.831 ± 0.247
0.529TrpPhe: 0.529 ± 0.209
1.209TrpGly: 1.209 ± 0.271
0.151TrpHis: 0.151 ± 0.097
0.831TrpIle: 0.831 ± 0.27
0.831TrpLys: 0.831 ± 0.267
1.133TrpLeu: 1.133 ± 0.343
0.227TrpMet: 0.227 ± 0.135
0.453TrpAsn: 0.453 ± 0.142
0.151TrpPro: 0.151 ± 0.095
0.604TrpGln: 0.604 ± 0.199
0.378TrpArg: 0.378 ± 0.138
0.302TrpSer: 0.302 ± 0.132
0.529TrpThr: 0.529 ± 0.179
0.68TrpVal: 0.68 ± 0.254
0.151TrpTrp: 0.151 ± 0.104
0.227TrpTyr: 0.227 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.116TyrAla: 2.116 ± 0.487
0.151TyrCys: 0.151 ± 0.112
2.342TyrAsp: 2.342 ± 0.496
3.022TyrGlu: 3.022 ± 0.623
2.116TyrPhe: 2.116 ± 0.485
2.418TyrGly: 2.418 ± 0.548
0.453TyrHis: 0.453 ± 0.159
2.191TyrIle: 2.191 ± 0.406
2.796TyrLys: 2.796 ± 0.553
3.098TyrLeu: 3.098 ± 0.568
0.68TyrMet: 0.68 ± 0.188
2.418TyrAsn: 2.418 ± 0.389
1.738TyrPro: 1.738 ± 0.452
2.116TyrGln: 2.116 ± 0.47
1.284TyrArg: 1.284 ± 0.272
3.551TyrSer: 3.551 ± 0.444
2.645TyrThr: 2.645 ± 0.522
2.04TyrVal: 2.04 ± 0.434
0.302TyrTrp: 0.302 ± 0.142
1.738TyrTyr: 1.738 ± 0.396
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (13236 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski