Bosea sp. LC85

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6190 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085EUM5|A0A085EUM5_9BRAD Lipid A biosynthesis acyltransferase OS=Bosea sp. LC85 OX=1502851 GN=FG93_06022 PE=4 SV=1
MM1 pKa = 6.67TLSYY5 pKa = 10.22EE6 pKa = 4.21VSDD9 pKa = 3.85DD10 pKa = 3.72TVAVAQTAVFSVVAFTEE27 pKa = 3.94IDD29 pKa = 3.35GTASDD34 pKa = 4.93DD35 pKa = 3.85SLTGTEE41 pKa = 4.54CADD44 pKa = 5.07LIDD47 pKa = 4.92GRR49 pKa = 11.84DD50 pKa = 3.78GNDD53 pKa = 3.53TIDD56 pKa = 3.36GRR58 pKa = 11.84GGSDD62 pKa = 2.96ILHH65 pKa = 6.57GGAGNDD71 pKa = 4.64FIRR74 pKa = 11.84GGTGHH79 pKa = 7.32DD80 pKa = 4.42LIYY83 pKa = 10.67AGRR86 pKa = 11.84GNDD89 pKa = 2.86IVYY92 pKa = 10.54GGTGNDD98 pKa = 3.97TIHH101 pKa = 6.87GGEE104 pKa = 4.86GDD106 pKa = 3.78DD107 pKa = 4.53QLFGEE112 pKa = 5.34DD113 pKa = 3.76GDD115 pKa = 5.31DD116 pKa = 4.48IIFGGDD122 pKa = 3.38GNDD125 pKa = 3.93LLVGGNGNDD134 pKa = 3.63QLSGGAGNDD143 pKa = 3.72VIQGGAGDD151 pKa = 4.12DD152 pKa = 4.14QIAGDD157 pKa = 3.93AGDD160 pKa = 4.59DD161 pKa = 3.79VVHH164 pKa = 6.86GDD166 pKa = 4.17EE167 pKa = 6.15GDD169 pKa = 3.61DD170 pKa = 3.72TLDD173 pKa = 3.61GGVGNDD179 pKa = 4.16EE180 pKa = 4.26LHH182 pKa = 6.97GDD184 pKa = 3.65AGDD187 pKa = 3.67DD188 pKa = 3.52HH189 pKa = 8.7VMGGAGDD196 pKa = 3.81DD197 pKa = 3.88MIYY200 pKa = 10.87GGDD203 pKa = 3.8GDD205 pKa = 4.02DD206 pKa = 4.75TIDD209 pKa = 4.05GGADD213 pKa = 3.27SDD215 pKa = 4.74EE216 pKa = 4.5IDD218 pKa = 4.48AGEE221 pKa = 4.22GDD223 pKa = 3.59NTIFTGDD230 pKa = 3.39GNNSVRR236 pKa = 11.84TGNGSNLVTGGAGMDD251 pKa = 3.39HH252 pKa = 6.15VQFGSDD258 pKa = 2.78QDD260 pKa = 4.0TARR263 pKa = 11.84LGAGDD268 pKa = 4.49DD269 pKa = 3.76VAAGGGGNDD278 pKa = 3.55RR279 pKa = 11.84LFGEE283 pKa = 5.05AGDD286 pKa = 4.2DD287 pKa = 3.9TLSGEE292 pKa = 4.51AGDD295 pKa = 5.1DD296 pKa = 3.81VLDD299 pKa = 4.7GGAGTDD305 pKa = 3.72GVEE308 pKa = 4.56AGSGDD313 pKa = 3.65DD314 pKa = 4.49RR315 pKa = 11.84IVGTLDD321 pKa = 3.25AAADD325 pKa = 4.16CYY327 pKa = 10.66MGGSGDD333 pKa = 3.74DD334 pKa = 3.52TLDD337 pKa = 3.38YY338 pKa = 11.29SQATQSIDD346 pKa = 3.08INLVEE351 pKa = 4.53GKK353 pKa = 10.35AVGVEE358 pKa = 3.64IGEE361 pKa = 4.89DD362 pKa = 3.45GFSSIEE368 pKa = 3.93AVLGGSGDD376 pKa = 3.62DD377 pKa = 3.87HH378 pKa = 6.01FTIGAEE384 pKa = 3.89AVTLSGGAGGDD395 pKa = 3.48CFSFDD400 pKa = 3.51VPYY403 pKa = 11.02DD404 pKa = 3.68ADD406 pKa = 4.16DD407 pKa = 4.74RR408 pKa = 11.84DD409 pKa = 4.86LIHH412 pKa = 7.28QILDD416 pKa = 3.52LEE418 pKa = 5.14AGDD421 pKa = 4.51RR422 pKa = 11.84IVIKK426 pKa = 10.13QYY428 pKa = 9.8QIRR431 pKa = 11.84TDD433 pKa = 3.97DD434 pKa = 4.68DD435 pKa = 3.84PTGDD439 pKa = 3.73AAGDD443 pKa = 3.79GADD446 pKa = 4.58PFASTYY452 pKa = 10.98GDD454 pKa = 3.41EE455 pKa = 4.9ADD457 pKa = 4.4AAGQPFRR464 pKa = 11.84FRR466 pKa = 11.84IEE468 pKa = 4.21KK469 pKa = 10.12IGEE472 pKa = 3.98DD473 pKa = 3.86DD474 pKa = 3.49YY475 pKa = 11.64TYY477 pKa = 11.4VDD479 pKa = 3.9VYY481 pKa = 11.4LEE483 pKa = 4.0QTDD486 pKa = 3.67EE487 pKa = 4.14KK488 pKa = 10.68DD489 pKa = 3.48YY490 pKa = 11.21SIEE493 pKa = 3.84IQGSHH498 pKa = 6.15RR499 pKa = 11.84LYY501 pKa = 11.01YY502 pKa = 9.82YY503 pKa = 11.18

Molecular weight: 51.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085FMT5|A0A085FMT5_9BRAD ABC transporter ATP-binding protein oligo/dipeptide transport protein OS=Bosea sp. LC85 OX=1502851 GN=FG93_02231 PE=3 SV=1
MM1 pKa = 7.45AADD4 pKa = 3.85KK5 pKa = 10.49PSRR8 pKa = 11.84APGRR12 pKa = 11.84LRR14 pKa = 11.84IVNPADD20 pKa = 3.19LTIRR24 pKa = 11.84RR25 pKa = 11.84VGRR28 pKa = 11.84GRR30 pKa = 11.84GFAYY34 pKa = 9.72IDD36 pKa = 3.9DD37 pKa = 4.48ADD39 pKa = 3.75ALVRR43 pKa = 11.84DD44 pKa = 3.87RR45 pKa = 11.84ATLEE49 pKa = 4.2RR50 pKa = 11.84IRR52 pKa = 11.84SLVIPPAYY60 pKa = 10.13CEE62 pKa = 3.95VRR64 pKa = 11.84IATDD68 pKa = 3.39PRR70 pKa = 11.84THH72 pKa = 6.25LQAIGRR78 pKa = 11.84DD79 pKa = 3.36EE80 pKa = 4.47AGRR83 pKa = 11.84LQYY86 pKa = 10.59LYY88 pKa = 9.89HH89 pKa = 6.94PEE91 pKa = 3.73WEE93 pKa = 4.19RR94 pKa = 11.84VRR96 pKa = 11.84EE97 pKa = 3.87RR98 pKa = 11.84RR99 pKa = 11.84KK100 pKa = 10.1LKK102 pKa = 10.09RR103 pKa = 11.84LRR105 pKa = 11.84RR106 pKa = 11.84LIEE109 pKa = 3.8ALPRR113 pKa = 11.84LRR115 pKa = 11.84RR116 pKa = 11.84AVADD120 pKa = 3.94DD121 pKa = 4.1LKK123 pKa = 11.24ARR125 pKa = 11.84PLCRR129 pKa = 11.84RR130 pKa = 11.84KK131 pKa = 10.13ALACAVAIIDD141 pKa = 3.89RR142 pKa = 11.84CHH144 pKa = 6.67LRR146 pKa = 11.84IGGEE150 pKa = 4.16PYY152 pKa = 10.6AKK154 pKa = 10.56SNGSHH159 pKa = 6.47GASTLLKK166 pKa = 10.03RR167 pKa = 11.84HH168 pKa = 5.69VEE170 pKa = 4.11LTASRR175 pKa = 11.84IDD177 pKa = 3.34LLFRR181 pKa = 11.84GKK183 pKa = 10.28GGKK186 pKa = 9.24QIACSLQDD194 pKa = 3.32GSLARR199 pKa = 11.84ALGRR203 pKa = 11.84IAALPGRR210 pKa = 11.84RR211 pKa = 11.84LLQYY215 pKa = 10.31RR216 pKa = 11.84DD217 pKa = 3.65DD218 pKa = 5.37DD219 pKa = 4.17GTVVPVHH226 pKa = 6.35AADD229 pKa = 3.04INAYY233 pKa = 9.43LRR235 pKa = 11.84EE236 pKa = 4.03VSGLAISAKK245 pKa = 9.14DD246 pKa = 3.54LRR248 pKa = 11.84MLAGNAAAAEE258 pKa = 4.32LLLASQIITGDD269 pKa = 3.48GSEE272 pKa = 3.98KK273 pKa = 10.25RR274 pKa = 11.84QLAEE278 pKa = 3.52VMRR281 pKa = 11.84AVSEE285 pKa = 4.12QLANTPAVTRR295 pKa = 11.84KK296 pKa = 9.97SYY298 pKa = 10.06VHH300 pKa = 6.91AIVVNAYY307 pKa = 10.69ANGKK311 pKa = 8.82LAASFEE317 pKa = 4.37KK318 pKa = 10.6VRR320 pKa = 11.84AGGGCSRR327 pKa = 11.84IEE329 pKa = 3.68RR330 pKa = 11.84ALALLAAA337 pKa = 5.3

Molecular weight: 36.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6190

0

6190

1918328

29

4390

309.9

33.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.137 ± 0.045

0.812 ± 0.011

5.288 ± 0.033

5.44 ± 0.03

3.728 ± 0.022

8.856 ± 0.04

1.952 ± 0.016

5.22 ± 0.02

3.07 ± 0.031

10.519 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.017

2.376 ± 0.021

5.362 ± 0.024

3.055 ± 0.018

7.323 ± 0.032

5.454 ± 0.02

5.214 ± 0.025

7.432 ± 0.027

1.315 ± 0.013

2.074 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski