Amino acid dipepetide frequency for Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.371AlaAla: 11.371 ± 0.144	1.173AlaCys: 1.173 ± 0.03	5.262AlaAsp: 5.262 ± 0.075	5.662AlaGlu: 5.662 ± 0.084	3.831AlaPhe: 3.831 ± 0.066	8.219AlaGly: 8.219 ± 0.101	2.136AlaHis: 2.136 ± 0.043	5.904AlaIle: 5.904 ± 0.084	3.68AlaLys: 3.68 ± 0.063	12.13AlaLeu: 12.13 ± 0.131	2.983AlaMet: 2.983 ± 0.055	3.026AlaAsn: 3.026 ± 0.053	4.003AlaPro: 4.003 ± 0.068	4.779AlaGln: 4.779 ± 0.068	6.085AlaArg: 6.085 ± 0.087	5.523AlaSer: 5.523 ± 0.073	4.723AlaThr: 4.723 ± 0.062	7.235AlaVal: 7.235 ± 0.091	1.756AlaTrp: 1.756 ± 0.041	1.904AlaTyr: 1.904 ± 0.037	0.0AlaXaa: 0.0 ± 0.0
Cys	1.048CysAla: 1.048 ± 0.036	0.206CysCys: 0.206 ± 0.013	0.567CysAsp: 0.567 ± 0.023	0.539CysGlu: 0.539 ± 0.021	0.503CysPhe: 0.503 ± 0.021	1.018CysGly: 1.018 ± 0.029	0.348CysHis: 0.348 ± 0.018	0.581CysIle: 0.581 ± 0.022	0.328CysLys: 0.328 ± 0.015	1.032CysLeu: 1.032 ± 0.029	0.256CysMet: 0.256 ± 0.015	0.34CysAsn: 0.34 ± 0.015	0.485CysPro: 0.485 ± 0.019	0.473CysGln: 0.473 ± 0.02	0.766CysArg: 0.766 ± 0.024	0.693CysSer: 0.693 ± 0.025	0.475CysThr: 0.475 ± 0.02	0.714CysVal: 0.714 ± 0.025	0.183CysTrp: 0.183 ± 0.011	0.337CysTyr: 0.337 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	5.139AspAla: 5.139 ± 0.071	0.456AspCys: 0.456 ± 0.019	2.941AspAsp: 2.941 ± 0.051	3.148AspGlu: 3.148 ± 0.055	2.104AspPhe: 2.104 ± 0.041	3.749AspGly: 3.749 ± 0.06	1.108AspHis: 1.108 ± 0.031	3.317AspIle: 3.317 ± 0.055	2.367AspLys: 2.367 ± 0.052	4.636AspLeu: 4.636 ± 0.069	1.194AspMet: 1.194 ± 0.028	2.171AspAsn: 2.171 ± 0.043	2.306AspPro: 2.306 ± 0.044	1.718AspGln: 1.718 ± 0.041	2.985AspArg: 2.985 ± 0.051	2.675AspSer: 2.675 ± 0.051	2.345AspThr: 2.345 ± 0.044	3.508AspVal: 3.508 ± 0.064	0.768AspTrp: 0.768 ± 0.028	1.724AspTyr: 1.724 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	5.272GluAla: 5.272 ± 0.072	0.413GluCys: 0.413 ± 0.019	2.18GluAsp: 2.18 ± 0.045	3.087GluGlu: 3.087 ± 0.06	1.658GluPhe: 1.658 ± 0.036	3.434GluGly: 3.434 ± 0.051	1.358GluHis: 1.358 ± 0.035	2.98GluIle: 2.98 ± 0.059	2.921GluLys: 2.921 ± 0.055	5.224GluLeu: 5.224 ± 0.076	1.615GluMet: 1.615 ± 0.034	2.102GluAsn: 2.102 ± 0.047	1.948GluPro: 1.948 ± 0.039	3.139GluGln: 3.139 ± 0.053	3.502GluArg: 3.502 ± 0.06	2.703GluSer: 2.703 ± 0.048	2.689GluThr: 2.689 ± 0.052	3.443GluVal: 3.443 ± 0.053	0.711GluTrp: 0.711 ± 0.028	1.338GluTyr: 1.338 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.012PheAla: 4.012 ± 0.065	0.575PheCys: 0.575 ± 0.023	2.253PheAsp: 2.253 ± 0.047	1.58PheGlu: 1.58 ± 0.038	1.93PhePhe: 1.93 ± 0.043	3.16PheGly: 3.16 ± 0.057	1.014PheHis: 1.014 ± 0.028	2.747PheIle: 2.747 ± 0.046	1.192PheLys: 1.192 ± 0.036	3.578PheLeu: 3.578 ± 0.06	1.006PheMet: 1.006 ± 0.028	1.756PheAsn: 1.756 ± 0.037	1.612PhePro: 1.612 ± 0.038	1.332PheGln: 1.332 ± 0.032	2.172PheArg: 2.172 ± 0.041	3.16PheSer: 3.16 ± 0.055	2.581PheThr: 2.581 ± 0.048	2.562PheVal: 2.562 ± 0.044	0.627PheTrp: 0.627 ± 0.023	1.222PheTyr: 1.222 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	6.554GlyAla: 6.554 ± 0.077	0.916GlyCys: 0.916 ± 0.032	3.886GlyAsp: 3.886 ± 0.062	4.556GlyGlu: 4.556 ± 0.066	3.317GlyPhe: 3.317 ± 0.055	5.777GlyGly: 5.777 ± 0.094	1.76GlyHis: 1.76 ± 0.045	4.969GlyIle: 4.969 ± 0.072	3.737GlyLys: 3.737 ± 0.064	7.639GlyLeu: 7.639 ± 0.085	2.383GlyMet: 2.383 ± 0.043	2.684GlyAsn: 2.684 ± 0.055	2.105GlyPro: 2.105 ± 0.043	3.125GlyGln: 3.125 ± 0.053	4.002GlyArg: 4.002 ± 0.056	4.128GlySer: 4.128 ± 0.061	3.517GlyThr: 3.517 ± 0.053	5.798GlyVal: 5.798 ± 0.072	1.262GlyTrp: 1.262 ± 0.03	2.473GlyTyr: 2.473 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.125HisAla: 2.125 ± 0.046	0.334HisCys: 0.334 ± 0.017	1.274HisAsp: 1.274 ± 0.036	1.039HisGlu: 1.039 ± 0.026	1.293HisPhe: 1.293 ± 0.034	1.855HisGly: 1.855 ± 0.042	1.149HisHis: 1.149 ± 0.044	1.442HisIle: 1.442 ± 0.035	0.827HisLys: 0.827 ± 0.025	2.564HisLeu: 2.564 ± 0.051	0.533HisMet: 0.533 ± 0.023	0.868HisAsn: 0.868 ± 0.025	1.501HisPro: 1.501 ± 0.035	1.536HisGln: 1.536 ± 0.041	1.804HisArg: 1.804 ± 0.051	1.339HisSer: 1.339 ± 0.033	1.123HisThr: 1.123 ± 0.029	1.385HisVal: 1.385 ± 0.033	0.392HisTrp: 0.392 ± 0.02	0.883HisTyr: 0.883 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.622IleAla: 6.622 ± 0.078	0.696IleCys: 0.696 ± 0.025	3.435IleAsp: 3.435 ± 0.051	2.926IleGlu: 2.926 ± 0.05	2.307IlePhe: 2.307 ± 0.048	4.529IleGly: 4.529 ± 0.062	1.315IleHis: 1.315 ± 0.033	3.368IleIle: 3.368 ± 0.067	2.358IleLys: 2.358 ± 0.057	4.938IleLeu: 4.938 ± 0.07	1.276IleMet: 1.276 ± 0.036	2.656IleAsn: 2.656 ± 0.055	2.852IlePro: 2.852 ± 0.053	1.891IleGln: 1.891 ± 0.037	2.967IleArg: 2.967 ± 0.049	3.707IleSer: 3.707 ± 0.06	3.328IleThr: 3.328 ± 0.065	3.85IleVal: 3.85 ± 0.06	0.64IleTrp: 0.64 ± 0.022	1.514IleTyr: 1.514 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	4.103LysAla: 4.103 ± 0.067	0.289LysCys: 0.289 ± 0.017	1.844LysAsp: 1.844 ± 0.047	2.17LysGlu: 2.17 ± 0.05	1.05LysPhe: 1.05 ± 0.033	2.745LysGly: 2.745 ± 0.051	0.873LysHis: 0.873 ± 0.031	2.284LysIle: 2.284 ± 0.044	2.17LysLys: 2.17 ± 0.052	3.951LysLeu: 3.951 ± 0.064	1.199LysMet: 1.199 ± 0.032	1.706LysAsn: 1.706 ± 0.045	1.999LysPro: 1.999 ± 0.042	1.905LysGln: 1.905 ± 0.042	2.491LysArg: 2.491 ± 0.045	2.188LysSer: 2.188 ± 0.048	2.462LysThr: 2.462 ± 0.045	2.766LysVal: 2.766 ± 0.055	0.41LysTrp: 0.41 ± 0.017	1.119LysTyr: 1.119 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	11.95LeuAla: 11.95 ± 0.158	1.314LeuCys: 1.314 ± 0.032	4.967LeuAsp: 4.967 ± 0.065	4.825LeuGlu: 4.825 ± 0.08	4.596LeuPhe: 4.596 ± 0.068	7.116LeuGly: 7.116 ± 0.086	2.598LeuHis: 2.598 ± 0.052	5.889LeuIle: 5.889 ± 0.08	4.289LeuLys: 4.289 ± 0.068	12.272LeuLeu: 12.272 ± 0.168	2.924LeuMet: 2.924 ± 0.052	4.16LeuAsn: 4.16 ± 0.061	6.006LeuPro: 6.006 ± 0.077	4.652LeuGln: 4.652 ± 0.069	6.657LeuArg: 6.657 ± 0.097	7.259LeuSer: 7.259 ± 0.087	6.478LeuThr: 6.478 ± 0.079	6.684LeuVal: 6.684 ± 0.079	1.419LeuTrp: 1.419 ± 0.043	2.546LeuTyr: 2.546 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	3.009MetAla: 3.009 ± 0.052	0.223MetCys: 0.223 ± 0.013	1.069MetAsp: 1.069 ± 0.032	1.122MetGlu: 1.122 ± 0.027	0.894MetPhe: 0.894 ± 0.029	1.859MetGly: 1.859 ± 0.043	0.584MetHis: 0.584 ± 0.021	1.466MetIle: 1.466 ± 0.035	1.293MetLys: 1.293 ± 0.032	3.15MetLeu: 3.15 ± 0.061	0.911MetMet: 0.911 ± 0.031	1.055MetAsn: 1.055 ± 0.031	1.469MetPro: 1.469 ± 0.035	1.166MetGln: 1.166 ± 0.029	1.575MetArg: 1.575 ± 0.037	1.735MetSer: 1.735 ± 0.033	1.784MetThr: 1.784 ± 0.039	1.944MetVal: 1.944 ± 0.039	0.254MetTrp: 0.254 ± 0.016	0.503MetTyr: 0.503 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.359AsnAla: 3.359 ± 0.053	0.351AsnCys: 0.351 ± 0.017	2.042AsnAsp: 2.042 ± 0.039	1.715AsnGlu: 1.715 ± 0.037	1.341AsnPhe: 1.341 ± 0.038	2.927AsnGly: 2.927 ± 0.051	0.953AsnHis: 0.953 ± 0.032	2.332AsnIle: 2.332 ± 0.05	1.591AsnLys: 1.591 ± 0.039	3.377AsnLeu: 3.377 ± 0.052	0.954AsnMet: 0.954 ± 0.029	1.643AsnAsn: 1.643 ± 0.047	2.064AsnPro: 2.064 ± 0.04	1.651AsnGln: 1.651 ± 0.035	2.163AsnArg: 2.163 ± 0.042	1.927AsnSer: 1.927 ± 0.046	1.921AsnThr: 1.921 ± 0.048	2.441AsnVal: 2.441 ± 0.047	0.502AsnTrp: 0.502 ± 0.021	1.166AsnTyr: 1.166 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	5.18ProAla: 5.18 ± 0.066	0.451ProCys: 0.451 ± 0.018	2.668ProAsp: 2.668 ± 0.043	3.092ProGlu: 3.092 ± 0.051	1.962ProPhe: 1.962 ± 0.041	3.769ProGly: 3.769 ± 0.055	1.187ProHis: 1.187 ± 0.034	1.847ProIle: 1.847 ± 0.049	1.348ProLys: 1.348 ± 0.035	5.551ProLeu: 5.551 ± 0.078	1.081ProMet: 1.081 ± 0.026	1.238ProAsn: 1.238 ± 0.03	1.979ProPro: 1.979 ± 0.042	2.646ProGln: 2.646 ± 0.055	2.309ProArg: 2.309 ± 0.043	2.246ProSer: 2.246 ± 0.042	2.179ProThr: 2.179 ± 0.043	3.91ProVal: 3.91 ± 0.058	0.795ProTrp: 0.795 ± 0.024	1.149ProTyr: 1.149 ± 0.034	0.001ProXaa: 0.001 ± 0.001
Gln	5.127GlnAla: 5.127 ± 0.076	0.385GlnCys: 0.385 ± 0.016	1.726GlnAsp: 1.726 ± 0.036	2.095GlnGlu: 2.095 ± 0.041	1.525GlnPhe: 1.525 ± 0.038	3.259GlnGly: 3.259 ± 0.057	1.531GlnHis: 1.531 ± 0.037	2.359GlnIle: 2.359 ± 0.048	1.727GlnLys: 1.727 ± 0.039	5.291GlnLeu: 5.291 ± 0.074	1.199GlnMet: 1.199 ± 0.03	1.453GlnAsn: 1.453 ± 0.037	2.48GlnPro: 2.48 ± 0.056	3.719GlnGln: 3.719 ± 0.082	3.976GlnArg: 3.976 ± 0.077	2.322GlnSer: 2.322 ± 0.046	2.338GlnThr: 2.338 ± 0.037	3.099GlnVal: 3.099 ± 0.05	0.608GlnTrp: 0.608 ± 0.021	1.218GlnTyr: 1.218 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	5.032ArgAla: 5.032 ± 0.075	0.683ArgCys: 0.683 ± 0.025	3.158ArgAsp: 3.158 ± 0.053	3.622ArgGlu: 3.622 ± 0.067	2.862ArgPhe: 2.862 ± 0.053	3.759ArgGly: 3.759 ± 0.06	2.125ArgHis: 2.125 ± 0.046	3.424ArgIle: 3.424 ± 0.052	2.315ArgLys: 2.315 ± 0.045	7.171ArgLeu: 7.171 ± 0.096	1.661ArgMet: 1.661 ± 0.038	2.114ArgAsn: 2.114 ± 0.041	2.653ArgPro: 2.653 ± 0.052	3.872ArgGln: 3.872 ± 0.074	4.525ArgArg: 4.525 ± 0.084	2.917ArgSer: 2.917 ± 0.056	2.515ArgThr: 2.515 ± 0.045	3.944ArgVal: 3.944 ± 0.06	1.014ArgTrp: 1.014 ± 0.032	2.225ArgTyr: 2.225 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	5.816SerAla: 5.816 ± 0.066	0.623SerCys: 0.623 ± 0.022	2.945SerAsp: 2.945 ± 0.051	2.819SerGlu: 2.819 ± 0.043	2.292SerPhe: 2.292 ± 0.044	5.069SerGly: 5.069 ± 0.078	1.485SerHis: 1.485 ± 0.042	2.985SerIle: 2.985 ± 0.057	1.842SerLys: 1.842 ± 0.041	6.757SerLeu: 6.757 ± 0.087	1.478SerMet: 1.478 ± 0.035	1.792SerAsn: 1.792 ± 0.046	2.817SerPro: 2.817 ± 0.045	2.576SerGln: 2.576 ± 0.047	3.574SerArg: 3.574 ± 0.064	3.523SerSer: 3.523 ± 0.071	2.924SerThr: 2.924 ± 0.054	4.061SerVal: 4.061 ± 0.059	1.003SerTrp: 1.003 ± 0.029	1.601SerTyr: 1.601 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	5.027ThrAla: 5.027 ± 0.067	0.542ThrCys: 0.542 ± 0.02	2.4ThrAsp: 2.4 ± 0.05	2.399ThrGlu: 2.399 ± 0.045	2.095ThrPhe: 2.095 ± 0.041	4.346ThrGly: 4.346 ± 0.07	1.207ThrHis: 1.207 ± 0.032	2.839ThrIle: 2.839 ± 0.049	1.444ThrLys: 1.444 ± 0.042	7.189ThrLeu: 7.189 ± 0.088	1.241ThrMet: 1.241 ± 0.033	1.496ThrAsn: 1.496 ± 0.04	3.22ThrPro: 3.22 ± 0.052	2.04ThrGln: 2.04 ± 0.047	3.184ThrArg: 3.184 ± 0.044	2.963ThrSer: 2.963 ± 0.058	2.93ThrThr: 2.93 ± 0.063	3.806ThrVal: 3.806 ± 0.061	0.809ThrTrp: 0.809 ± 0.027	1.125ThrTyr: 1.125 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	7.216ValAla: 7.216 ± 0.087	0.734ValCys: 0.734 ± 0.027	3.658ValAsp: 3.658 ± 0.054	3.727ValGlu: 3.727 ± 0.062	2.66ValPhe: 2.66 ± 0.05	4.938ValGly: 4.938 ± 0.066	1.348ValHis: 1.348 ± 0.036	4.387ValIle: 4.387 ± 0.067	2.903ValLys: 2.903 ± 0.048	6.895ValLeu: 6.895 ± 0.083	2.163ValMet: 2.163 ± 0.041	2.743ValAsn: 2.743 ± 0.055	2.969ValPro: 2.969 ± 0.047	2.499ValGln: 2.499 ± 0.047	3.747ValArg: 3.747 ± 0.055	4.409ValSer: 4.409 ± 0.061	4.015ValThr: 4.015 ± 0.069	5.432ValVal: 5.432 ± 0.078	0.942ValTrp: 0.942 ± 0.031	1.66ValTyr: 1.66 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	0.976TrpAla: 0.976 ± 0.029	0.168TrpCys: 0.168 ± 0.01	0.617TrpAsp: 0.617 ± 0.023	0.495TrpGlu: 0.495 ± 0.02	0.685TrpPhe: 0.685 ± 0.025	0.95TrpGly: 0.95 ± 0.028	0.421TrpHis: 0.421 ± 0.019	0.69TrpIle: 0.69 ± 0.023	0.477TrpLys: 0.477 ± 0.019	2.301TrpLeu: 2.301 ± 0.052	0.426TrpMet: 0.426 ± 0.016	0.488TrpAsn: 0.488 ± 0.022	0.704TrpPro: 0.704 ± 0.026	1.166TrpGln: 1.166 ± 0.033	1.228TrpArg: 1.228 ± 0.036	0.833TrpSer: 0.833 ± 0.03	0.602TrpThr: 0.602 ± 0.024	0.888TrpVal: 0.888 ± 0.03	0.225TrpTrp: 0.225 ± 0.014	0.397TrpTyr: 0.397 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.446TyrAla: 2.446 ± 0.044	0.359TyrCys: 0.359 ± 0.017	1.468TyrAsp: 1.468 ± 0.036	1.1TyrGlu: 1.1 ± 0.032	1.166TyrPhe: 1.166 ± 0.028	2.108TyrGly: 2.108 ± 0.047	0.766TyrHis: 0.766 ± 0.024	1.365TyrIle: 1.365 ± 0.04	0.915TyrLys: 0.915 ± 0.03	2.954TyrLeu: 2.954 ± 0.046	0.581TyrMet: 0.581 ± 0.023	0.928TyrAsn: 0.928 ± 0.026	1.415TyrPro: 1.415 ± 0.037	1.553TyrGln: 1.553 ± 0.041	1.88TyrArg: 1.88 ± 0.039	1.735TyrSer: 1.735 ± 0.043	1.372TyrThr: 1.372 ± 0.033	1.576TyrVal: 1.576 ± 0.03	0.42TyrTrp: 0.42 ± 0.018	0.944TyrTyr: 0.944 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4528 proteins (1291935 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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