Amino acid dipepetide frequency for Bacillus sp. FJAT-26390

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.042AlaAla: 10.042 ± 0.131
0.66AlaCys: 0.66 ± 0.02
4.611AlaAsp: 4.611 ± 0.056
5.754AlaGlu: 5.754 ± 0.07
3.6AlaPhe: 3.6 ± 0.046
6.846AlaGly: 6.846 ± 0.077
1.456AlaHis: 1.456 ± 0.029
6.018AlaIle: 6.018 ± 0.058
4.646AlaLys: 4.646 ± 0.051
8.448AlaLeu: 8.448 ± 0.066
2.265AlaMet: 2.265 ± 0.033
3.008AlaAsn: 3.008 ± 0.045
2.83AlaPro: 2.83 ± 0.043
2.711AlaGln: 2.711 ± 0.036
3.363AlaArg: 3.363 ± 0.049
5.724AlaSer: 5.724 ± 0.06
3.924AlaThr: 3.924 ± 0.075
6.572AlaVal: 6.572 ± 0.078
1.009AlaTrp: 1.009 ± 0.024
2.823AlaTyr: 2.823 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.471CysAla: 0.471 ± 0.017
0.103CysCys: 0.103 ± 0.007
0.341CysAsp: 0.341 ± 0.013
0.402CysGlu: 0.402 ± 0.013
0.301CysPhe: 0.301 ± 0.011
0.674CysGly: 0.674 ± 0.019
0.165CysHis: 0.165 ± 0.011
0.437CysIle: 0.437 ± 0.014
0.322CysLys: 0.322 ± 0.015
0.655CysLeu: 0.655 ± 0.021
0.199CysMet: 0.199 ± 0.009
0.225CysAsn: 0.225 ± 0.011
0.295CysPro: 0.295 ± 0.012
0.19CysGln: 0.19 ± 0.01
0.343CysArg: 0.343 ± 0.013
0.549CysSer: 0.549 ± 0.018
0.334CysThr: 0.334 ± 0.013
0.414CysVal: 0.414 ± 0.014
0.09CysTrp: 0.09 ± 0.007
0.238CysTyr: 0.238 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
4.247AspAla: 4.247 ± 0.052
0.315AspCys: 0.315 ± 0.011
2.437AspAsp: 2.437 ± 0.039
3.827AspGlu: 3.827 ± 0.046
2.19AspPhe: 2.19 ± 0.034
4.233AspGly: 4.233 ± 0.054
1.134AspHis: 1.134 ± 0.025
3.51AspIle: 3.51 ± 0.039
2.89AspLys: 2.89 ± 0.049
4.641AspLeu: 4.641 ± 0.055
1.363AspMet: 1.363 ± 0.026
1.938AspAsn: 1.938 ± 0.032
2.198AspPro: 2.198 ± 0.036
1.946AspGln: 1.946 ± 0.031
2.633AspArg: 2.633 ± 0.041
2.914AspSer: 2.914 ± 0.044
2.574AspThr: 2.574 ± 0.041
3.494AspVal: 3.494 ± 0.046
0.885AspTrp: 0.885 ± 0.022
2.105AspTyr: 2.105 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
6.298GluAla: 6.298 ± 0.065
0.33GluCys: 0.33 ± 0.014
2.98GluAsp: 2.98 ± 0.041
4.836GluGlu: 4.836 ± 0.065
1.941GluPhe: 1.941 ± 0.034
4.261GluGly: 4.261 ± 0.049
1.604GluHis: 1.604 ± 0.029
4.026GluIle: 4.026 ± 0.051
3.89GluLys: 3.89 ± 0.053
7.454GluLeu: 7.454 ± 0.091
1.894GluMet: 1.894 ± 0.031
2.418GluAsn: 2.418 ± 0.039
2.285GluPro: 2.285 ± 0.047
4.018GluGln: 4.018 ± 0.062
3.87GluArg: 3.87 ± 0.056
3.609GluSer: 3.609 ± 0.043
3.227GluThr: 3.227 ± 0.045
4.117GluVal: 4.117 ± 0.049
1.015GluTrp: 1.015 ± 0.021
1.871GluTyr: 1.871 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.43PheAla: 3.43 ± 0.044
0.326PheCys: 0.326 ± 0.013
2.375PheAsp: 2.375 ± 0.039
2.582PheGlu: 2.582 ± 0.034
1.819PhePhe: 1.819 ± 0.038
3.297PheGly: 3.297 ± 0.047
0.898PheHis: 0.898 ± 0.022
3.085PheIle: 3.085 ± 0.052
2.212PheLys: 2.212 ± 0.035
3.751PheLeu: 3.751 ± 0.059
1.182PheMet: 1.182 ± 0.027
1.79PheAsn: 1.79 ± 0.034
1.624PhePro: 1.624 ± 0.027
1.448PheGln: 1.448 ± 0.028
1.888PheArg: 1.888 ± 0.028
2.906PheSer: 2.906 ± 0.043
2.511PheThr: 2.511 ± 0.038
3.143PheVal: 3.143 ± 0.044
0.556PheTrp: 0.556 ± 0.015
1.496PheTyr: 1.496 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
5.746GlyAla: 5.746 ± 0.099
0.626GlyCys: 0.626 ± 0.02
3.479GlyAsp: 3.479 ± 0.048
4.506GlyGlu: 4.506 ± 0.055
3.235GlyPhe: 3.235 ± 0.042
5.426GlyGly: 5.426 ± 0.096
1.415GlyHis: 1.415 ± 0.023
5.677GlyIle: 5.677 ± 0.056
4.683GlyLys: 4.683 ± 0.053
6.988GlyLeu: 6.988 ± 0.065
2.251GlyMet: 2.251 ± 0.031
2.902GlyAsn: 2.902 ± 0.047
1.825GlyPro: 1.825 ± 0.034
2.614GlyGln: 2.614 ± 0.042
3.12GlyArg: 3.12 ± 0.041
4.931GlySer: 4.931 ± 0.064
4.358GlyThr: 4.358 ± 0.06
4.968GlyVal: 4.968 ± 0.052
1.12GlyTrp: 1.12 ± 0.028
2.908GlyTyr: 2.908 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
1.715HisAla: 1.715 ± 0.028
0.169HisCys: 0.169 ± 0.009
1.0HisAsp: 1.0 ± 0.022
1.281HisGlu: 1.281 ± 0.023
1.146HisPhe: 1.146 ± 0.025
1.425HisGly: 1.425 ± 0.029
0.602HisHis: 0.602 ± 0.02
1.424HisIle: 1.424 ± 0.027
0.884HisLys: 0.884 ± 0.02
2.11HisLeu: 2.11 ± 0.038
0.562HisMet: 0.562 ± 0.018
0.68HisAsn: 0.68 ± 0.019
1.099HisPro: 1.099 ± 0.024
0.696HisGln: 0.696 ± 0.019
0.953HisArg: 0.953 ± 0.021
1.178HisSer: 1.178 ± 0.023
1.059HisThr: 1.059 ± 0.022
1.379HisVal: 1.379 ± 0.027
0.322HisTrp: 0.322 ± 0.01
0.896HisTyr: 0.896 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.373IleAla: 6.373 ± 0.057
0.58IleCys: 0.58 ± 0.018
3.942IleAsp: 3.942 ± 0.044
4.503IleGlu: 4.503 ± 0.055
2.46IlePhe: 2.46 ± 0.044
5.551IleGly: 5.551 ± 0.064
1.463IleHis: 1.463 ± 0.03
4.729IleIle: 4.729 ± 0.06
3.373IleLys: 3.373 ± 0.043
5.566IleLeu: 5.566 ± 0.071
1.749IleMet: 1.749 ± 0.031
2.72IleAsn: 2.72 ± 0.038
3.071IlePro: 3.071 ± 0.044
2.45IleGln: 2.45 ± 0.03
3.56IleArg: 3.56 ± 0.045
4.74IleSer: 4.74 ± 0.053
3.961IleThr: 3.961 ± 0.045
5.432IleVal: 5.432 ± 0.054
0.779IleTrp: 0.779 ± 0.02
2.136IleTyr: 2.136 ± 0.034
0.0IleXaa: 0.0 ± 0.001
Lys
4.669LysAla: 4.669 ± 0.052
0.217LysCys: 0.217 ± 0.011
2.973LysAsp: 2.973 ± 0.047
4.271LysGlu: 4.271 ± 0.045
1.642LysPhe: 1.642 ± 0.028
3.679LysGly: 3.679 ± 0.049
1.192LysHis: 1.192 ± 0.026
3.257LysIle: 3.257 ± 0.043
3.298LysLys: 3.298 ± 0.047
6.037LysLeu: 6.037 ± 0.058
1.665LysMet: 1.665 ± 0.028
2.178LysAsn: 2.178 ± 0.036
2.429LysPro: 2.429 ± 0.035
2.897LysGln: 2.897 ± 0.043
2.9LysArg: 2.9 ± 0.041
3.107LysSer: 3.107 ± 0.042
2.923LysThr: 2.923 ± 0.041
3.6LysVal: 3.6 ± 0.045
0.821LysTrp: 0.821 ± 0.023
1.79LysTyr: 1.79 ± 0.03
0.001LysXaa: 0.001 ± 0.001
Leu
8.61LeuAla: 8.61 ± 0.079
0.738LeuCys: 0.738 ± 0.019
5.054LeuAsp: 5.054 ± 0.055
6.136LeuGlu: 6.136 ± 0.072
4.741LeuPhe: 4.741 ± 0.07
6.561LeuGly: 6.561 ± 0.056
2.024LeuHis: 2.024 ± 0.035
6.711LeuIle: 6.711 ± 0.077
5.499LeuLys: 5.499 ± 0.05
11.17LeuLeu: 11.17 ± 0.106
2.661LeuMet: 2.661 ± 0.036
3.967LeuAsn: 3.967 ± 0.047
4.442LeuPro: 4.442 ± 0.045
4.147LeuGln: 4.147 ± 0.052
4.679LeuArg: 4.679 ± 0.055
7.186LeuSer: 7.186 ± 0.065
5.685LeuThr: 5.685 ± 0.058
6.256LeuVal: 6.256 ± 0.064
1.069LeuTrp: 1.069 ± 0.026
3.178LeuTyr: 3.178 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
2.155MetAla: 2.155 ± 0.032
0.132MetCys: 0.132 ± 0.008
1.45MetAsp: 1.45 ± 0.025
1.782MetGlu: 1.782 ± 0.032
1.073MetPhe: 1.073 ± 0.026
1.635MetGly: 1.635 ± 0.03
0.502MetHis: 0.502 ± 0.015
1.981MetIle: 1.981 ± 0.033
1.982MetLys: 1.982 ± 0.03
3.093MetLeu: 3.093 ± 0.043
0.845MetMet: 0.845 ± 0.023
1.486MetAsn: 1.486 ± 0.027
1.197MetPro: 1.197 ± 0.027
1.029MetGln: 1.029 ± 0.024
1.3MetArg: 1.3 ± 0.024
1.818MetSer: 1.818 ± 0.026
1.691MetThr: 1.691 ± 0.03
1.693MetVal: 1.693 ± 0.03
0.237MetTrp: 0.237 ± 0.011
0.752MetTyr: 0.752 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.247AsnAla: 3.247 ± 0.045
0.224AsnCys: 0.224 ± 0.012
2.186AsnAsp: 2.186 ± 0.035
2.893AsnGlu: 2.893 ± 0.043
1.342AsnPhe: 1.342 ± 0.031
3.588AsnGly: 3.588 ± 0.056
0.898AsnHis: 0.898 ± 0.022
2.447AsnIle: 2.447 ± 0.039
2.42AsnLys: 2.42 ± 0.041
3.202AsnLeu: 3.202 ± 0.044
1.068AsnMet: 1.068 ± 0.024
1.75AsnAsn: 1.75 ± 0.038
1.983AsnPro: 1.983 ± 0.032
1.558AsnGln: 1.558 ± 0.03
2.097AsnArg: 2.097 ± 0.031
2.237AsnSer: 2.237 ± 0.041
2.017AsnThr: 2.017 ± 0.037
2.762AsnVal: 2.762 ± 0.043
0.591AsnTrp: 0.591 ± 0.018
1.444AsnTyr: 1.444 ± 0.036
0.001AsnXaa: 0.001 ± 0.001
Pro
3.455ProAla: 3.455 ± 0.044
0.22ProCys: 0.22 ± 0.011
2.345ProAsp: 2.345 ± 0.034
2.995ProGlu: 2.995 ± 0.043
1.963ProPhe: 1.963 ± 0.032
2.561ProGly: 2.561 ± 0.037
0.831ProHis: 0.831 ± 0.022
2.766ProIle: 2.766 ± 0.036
1.815ProLys: 1.815 ± 0.034
3.945ProLeu: 3.945 ± 0.047
0.916ProMet: 0.916 ± 0.023
1.691ProAsn: 1.691 ± 0.032
1.162ProPro: 1.162 ± 0.024
1.344ProGln: 1.344 ± 0.024
1.273ProArg: 1.273 ± 0.028
2.706ProSer: 2.706 ± 0.041
2.078ProThr: 2.078 ± 0.05
2.96ProVal: 2.96 ± 0.046
0.516ProTrp: 0.516 ± 0.015
1.485ProTyr: 1.485 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.533GlnAla: 3.533 ± 0.05
0.16GlnCys: 0.16 ± 0.01
1.563GlnAsp: 1.563 ± 0.025
2.342GlnGlu: 2.342 ± 0.042
1.651GlnPhe: 1.651 ± 0.03
2.404GlnGly: 2.404 ± 0.035
0.776GlnHis: 0.776 ± 0.02
2.551GlnIle: 2.551 ± 0.033
2.046GlnLys: 2.046 ± 0.027
4.631GlnLeu: 4.631 ± 0.064
1.196GlnMet: 1.196 ± 0.025
1.464GlnAsn: 1.464 ± 0.029
1.548GlnPro: 1.548 ± 0.028
1.991GlnGln: 1.991 ± 0.04
1.772GlnArg: 1.772 ± 0.038
2.507GlnSer: 2.507 ± 0.039
2.151GlnThr: 2.151 ± 0.035
2.464GlnVal: 2.464 ± 0.037
0.536GlnTrp: 0.536 ± 0.017
1.373GlnTyr: 1.373 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
3.214ArgAla: 3.214 ± 0.044
0.308ArgCys: 0.308 ± 0.013
2.232ArgAsp: 2.232 ± 0.035
3.297ArgGlu: 3.297 ± 0.053
2.274ArgPhe: 2.274 ± 0.035
2.807ArgGly: 2.807 ± 0.042
0.979ArgHis: 0.979 ± 0.024
3.469ArgIle: 3.469 ± 0.043
2.881ArgLys: 2.881 ± 0.035
5.021ArgLeu: 5.021 ± 0.059
1.628ArgMet: 1.628 ± 0.029
1.903ArgAsn: 1.903 ± 0.031
1.513ArgPro: 1.513 ± 0.029
1.82ArgGln: 1.82 ± 0.033
2.312ArgArg: 2.312 ± 0.038
3.118ArgSer: 3.118 ± 0.04
2.502ArgThr: 2.502 ± 0.033
2.929ArgVal: 2.929 ± 0.04
0.613ArgTrp: 0.613 ± 0.019
1.809ArgTyr: 1.809 ± 0.033
0.001ArgXaa: 0.001 ± 0.001
Ser
5.217SerAla: 5.217 ± 0.057
0.424SerCys: 0.424 ± 0.014
3.367SerAsp: 3.367 ± 0.038
4.067SerGlu: 4.067 ± 0.043
3.272SerPhe: 3.272 ± 0.042
5.436SerGly: 5.436 ± 0.06
1.284SerHis: 1.284 ± 0.026
4.758SerIle: 4.758 ± 0.049
3.503SerLys: 3.503 ± 0.043
6.626SerLeu: 6.626 ± 0.065
1.787SerMet: 1.787 ± 0.035
2.53SerAsn: 2.53 ± 0.044
2.399SerPro: 2.399 ± 0.036
2.014SerGln: 2.014 ± 0.029
2.863SerArg: 2.863 ± 0.043
4.676SerSer: 4.676 ± 0.058
3.199SerThr: 3.199 ± 0.044
4.612SerVal: 4.612 ± 0.061
0.878SerTrp: 0.878 ± 0.022
2.423SerTyr: 2.423 ± 0.036
0.001SerXaa: 0.001 ± 0.001
Thr
5.028ThrAla: 5.028 ± 0.062
0.293ThrCys: 0.293 ± 0.012
2.936ThrAsp: 2.936 ± 0.044
3.255ThrGlu: 3.255 ± 0.062
2.464ThrPhe: 2.464 ± 0.04
4.399ThrGly: 4.399 ± 0.099
0.957ThrHis: 0.957 ± 0.024
4.201ThrIle: 4.201 ± 0.052
2.653ThrLys: 2.653 ± 0.041
5.361ThrLeu: 5.361 ± 0.057
1.292ThrMet: 1.292 ± 0.028
2.193ThrAsn: 2.193 ± 0.034
2.389ThrPro: 2.389 ± 0.038
1.519ThrGln: 1.519 ± 0.029
2.022ThrArg: 2.022 ± 0.029
3.34ThrSer: 3.34 ± 0.045
2.908ThrThr: 2.908 ± 0.056
4.388ThrVal: 4.388 ± 0.053
0.651ThrTrp: 0.651 ± 0.018
1.841ThrTyr: 1.841 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
5.247ValAla: 5.247 ± 0.058
0.549ValCys: 0.549 ± 0.018
3.67ValAsp: 3.67 ± 0.049
4.091ValGlu: 4.091 ± 0.051
2.947ValPhe: 2.947 ± 0.043
4.456ValGly: 4.456 ± 0.052
1.415ValHis: 1.415 ± 0.025
5.099ValIle: 5.099 ± 0.054
3.979ValLys: 3.979 ± 0.052
6.988ValLeu: 6.988 ± 0.065
1.915ValMet: 1.915 ± 0.033
2.923ValAsn: 2.923 ± 0.05
2.87ValPro: 2.87 ± 0.04
2.543ValGln: 2.543 ± 0.041
3.18ValArg: 3.18 ± 0.042
4.844ValSer: 4.844 ± 0.055
4.435ValThr: 4.435 ± 0.066
4.829ValVal: 4.829 ± 0.068
0.849ValTrp: 0.849 ± 0.022
2.351ValTyr: 2.351 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
0.873TrpAla: 0.873 ± 0.023
0.089TrpCys: 0.089 ± 0.008
0.71TrpAsp: 0.71 ± 0.021
0.77TrpGlu: 0.77 ± 0.019
0.593TrpPhe: 0.593 ± 0.018
0.865TrpGly: 0.865 ± 0.022
0.277TrpHis: 0.277 ± 0.012
0.914TrpIle: 0.914 ± 0.022
0.772TrpLys: 0.772 ± 0.02
1.5TrpLeu: 1.5 ± 0.03
0.444TrpMet: 0.444 ± 0.014
0.699TrpAsn: 0.699 ± 0.02
0.372TrpPro: 0.372 ± 0.014
0.55TrpGln: 0.55 ± 0.017
0.624TrpArg: 0.624 ± 0.018
0.957TrpSer: 0.957 ± 0.024
0.729TrpThr: 0.729 ± 0.021
0.833TrpVal: 0.833 ± 0.023
0.187TrpTrp: 0.187 ± 0.009
0.441TrpTyr: 0.441 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.739TyrAla: 2.739 ± 0.04
0.285TyrCys: 0.285 ± 0.013
1.825TyrAsp: 1.825 ± 0.031
2.356TyrGlu: 2.356 ± 0.038
1.666TyrPhe: 1.666 ± 0.03
2.674TyrGly: 2.674 ± 0.037
0.71TyrHis: 0.71 ± 0.019
2.075TyrIle: 2.075 ± 0.038
1.797TyrLys: 1.797 ± 0.032
3.324TyrLeu: 3.324 ± 0.039
0.951TyrMet: 0.951 ± 0.023
1.488TyrAsn: 1.488 ± 0.03
1.496TyrPro: 1.496 ± 0.029
1.176TyrGln: 1.176 ± 0.023
1.877TyrArg: 1.877 ± 0.033
2.289TyrSer: 2.289 ± 0.033
1.823TyrThr: 1.823 ± 0.033
2.334TyrVal: 2.334 ± 0.033
0.474TyrTrp: 0.474 ± 0.016
1.364TyrTyr: 1.364 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.001XaaAsp: 0.001 ± 0.001
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.001XaaLys: 0.001 ± 0.001
0.002XaaLeu: 0.002 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6340 proteins (2129051 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski