Proteiniborus ethanoligenes

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Proteiniborus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2950 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3P8L5|A0A1H3P8L5_9FIRM EDD domain protein DegV family OS=Proteiniborus ethanoligenes OX=415015 GN=SAMN05660462_01399 PE=4 SV=1
MM1 pKa = 6.91SQEE4 pKa = 3.63ISSNIPVEE12 pKa = 4.09RR13 pKa = 11.84EE14 pKa = 3.98SFFGEE19 pKa = 4.13NNEE22 pKa = 4.02SLLFFFLILVIIFCNCGLFDD42 pKa = 4.84EE43 pKa = 5.06MNEE46 pKa = 3.91SLLFFFLLLIIIFCKK61 pKa = 10.72CFDD64 pKa = 3.66FF65 pKa = 6.35

Molecular weight: 7.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3RIG7|A0A1H3RIG7_9FIRM Proline--tRNA ligase OS=Proteiniborus ethanoligenes OX=415015 GN=proS PE=3 SV=1
MM1 pKa = 7.29AQPGRR6 pKa = 11.84RR7 pKa = 11.84FRR9 pKa = 11.84AKK11 pKa = 10.17KK12 pKa = 9.49RR13 pKa = 11.84VCSFCVDD20 pKa = 3.19NVDD23 pKa = 3.88KK24 pKa = 11.14INYY27 pKa = 9.45KK28 pKa = 9.94EE29 pKa = 3.97VNKK32 pKa = 10.11LKK34 pKa = 10.65KK35 pKa = 10.67YY36 pKa = 7.29ITEE39 pKa = 4.07RR40 pKa = 11.84GKK42 pKa = 10.04ILPRR46 pKa = 11.84RR47 pKa = 11.84ISGNCALHH55 pKa = 5.68QRR57 pKa = 11.84EE58 pKa = 4.32LTRR61 pKa = 11.84AIKK64 pKa = 10.25RR65 pKa = 11.84ARR67 pKa = 11.84QVAILPYY74 pKa = 9.79TADD77 pKa = 3.14

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2950

0

2950

922494

39

2752

312.7

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.814 ± 0.05

0.889 ± 0.018

5.543 ± 0.039

7.787 ± 0.058

4.15 ± 0.035

6.726 ± 0.051

1.509 ± 0.017

10.213 ± 0.057

8.353 ± 0.045

9.215 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.02

5.706 ± 0.033

3.036 ± 0.023

2.491 ± 0.019

3.79 ± 0.032

6.166 ± 0.035

4.935 ± 0.039

6.37 ± 0.039

0.686 ± 0.014

4.02 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski