Amino acid dipepetide frequency for Lachnospiraceae bacterium KH1T2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.507AlaAla: 7.507 ± 0.127	0.966AlaCys: 0.966 ± 0.03	4.981AlaAsp: 4.981 ± 0.067	6.261AlaGlu: 6.261 ± 0.077	2.994AlaPhe: 2.994 ± 0.053	5.704AlaGly: 5.704 ± 0.086	1.105AlaHis: 1.105 ± 0.035	5.167AlaIle: 5.167 ± 0.078	4.885AlaLys: 4.885 ± 0.072	6.483AlaLeu: 6.483 ± 0.084	2.246AlaMet: 2.246 ± 0.051	2.917AlaAsn: 2.917 ± 0.055	1.864AlaPro: 1.864 ± 0.044	1.876AlaGln: 1.876 ± 0.041	2.581AlaArg: 2.581 ± 0.052	4.614AlaSer: 4.614 ± 0.081	2.962AlaThr: 2.962 ± 0.057	5.914AlaVal: 5.914 ± 0.073	0.587AlaTrp: 0.587 ± 0.022	2.691AlaTyr: 2.691 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.971CysAla: 0.971 ± 0.028	0.248CysCys: 0.248 ± 0.016	0.856CysAsp: 0.856 ± 0.024	0.877CysGlu: 0.877 ± 0.029	0.635CysPhe: 0.635 ± 0.023	1.344CysGly: 1.344 ± 0.043	0.272CysHis: 0.272 ± 0.016	1.122CysIle: 1.122 ± 0.029	0.818CysLys: 0.818 ± 0.026	0.979CysLeu: 0.979 ± 0.028	0.376CysMet: 0.376 ± 0.021	0.577CysAsn: 0.577 ± 0.021	0.482CysPro: 0.482 ± 0.021	0.276CysGln: 0.276 ± 0.014	0.614CysArg: 0.614 ± 0.023	0.876CysSer: 0.876 ± 0.029	0.693CysThr: 0.693 ± 0.025	0.95CysVal: 0.95 ± 0.027	0.125CysTrp: 0.125 ± 0.011	0.512CysTyr: 0.512 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	4.433AspAla: 4.433 ± 0.062	0.767AspCys: 0.767 ± 0.026	4.135AspAsp: 4.135 ± 0.07	5.904AspGlu: 5.904 ± 0.072	3.031AspPhe: 3.031 ± 0.056	4.742AspGly: 4.742 ± 0.095	0.959AspHis: 0.959 ± 0.03	5.398AspIle: 5.398 ± 0.069	4.476AspLys: 4.476 ± 0.065	4.537AspLeu: 4.537 ± 0.065	1.932AspMet: 1.932 ± 0.039	2.818AspAsn: 2.818 ± 0.051	1.898AspPro: 1.898 ± 0.049	1.22AspGln: 1.22 ± 0.035	2.388AspArg: 2.388 ± 0.047	3.694AspSer: 3.694 ± 0.067	3.147AspThr: 3.147 ± 0.059	4.131AspVal: 4.131 ± 0.06	0.59AspTrp: 0.59 ± 0.024	3.103AspTyr: 3.103 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	5.835GluAla: 5.835 ± 0.087	0.87GluCys: 0.87 ± 0.029	4.575GluAsp: 4.575 ± 0.07	6.579GluGlu: 6.579 ± 0.101	2.948GluPhe: 2.948 ± 0.052	4.286GluGly: 4.286 ± 0.07	1.312GluHis: 1.312 ± 0.035	5.988GluIle: 5.988 ± 0.075	7.227GluLys: 7.227 ± 0.09	6.873GluLeu: 6.873 ± 0.089	2.26GluMet: 2.26 ± 0.046	4.852GluAsn: 4.852 ± 0.069	1.802GluPro: 1.802 ± 0.052	2.025GluGln: 2.025 ± 0.047	3.323GluArg: 3.323 ± 0.065	4.103GluSer: 4.103 ± 0.07	3.533GluThr: 3.533 ± 0.052	4.55GluVal: 4.55 ± 0.075	0.712GluTrp: 0.712 ± 0.029	3.323GluTyr: 3.323 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.071PheAla: 3.071 ± 0.052	0.699PheCys: 0.699 ± 0.026	2.984PheAsp: 2.984 ± 0.057	3.019PheGlu: 3.019 ± 0.05	2.012PhePhe: 2.012 ± 0.057	2.937PheGly: 2.937 ± 0.061	0.745PheHis: 0.745 ± 0.027	3.349PheIle: 3.349 ± 0.061	2.69PheLys: 2.69 ± 0.041	3.566PheLeu: 3.566 ± 0.067	1.282PheMet: 1.282 ± 0.036	2.128PheAsn: 2.128 ± 0.042	1.308PhePro: 1.308 ± 0.04	0.96PheGln: 0.96 ± 0.031	1.707PheArg: 1.707 ± 0.042	3.051PheSer: 3.051 ± 0.056	2.414PheThr: 2.414 ± 0.041	2.829PheVal: 2.829 ± 0.049	0.453PheTrp: 0.453 ± 0.021	1.875PheTyr: 1.875 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.69GlyAla: 4.69 ± 0.074	1.112GlyCys: 1.112 ± 0.035	3.704GlyAsp: 3.704 ± 0.058	4.582GlyGlu: 4.582 ± 0.07	3.236GlyPhe: 3.236 ± 0.057	4.339GlyGly: 4.339 ± 0.074	1.146GlyHis: 1.146 ± 0.032	5.988GlyIle: 5.988 ± 0.084	5.489GlyLys: 5.489 ± 0.088	5.222GlyLeu: 5.222 ± 0.073	2.274GlyMet: 2.274 ± 0.045	3.516GlyAsn: 3.516 ± 0.054	1.133GlyPro: 1.133 ± 0.036	1.576GlyGln: 1.576 ± 0.038	2.815GlyArg: 2.815 ± 0.05	4.167GlySer: 4.167 ± 0.063	4.076GlyThr: 4.076 ± 0.068	4.7GlyVal: 4.7 ± 0.072	0.659GlyTrp: 0.659 ± 0.026	3.186GlyTyr: 3.186 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.013HisAla: 1.013 ± 0.033	0.244HisCys: 0.244 ± 0.013	1.029HisAsp: 1.029 ± 0.03	1.127HisGlu: 1.127 ± 0.038	0.836HisPhe: 0.836 ± 0.03	1.192HisGly: 1.192 ± 0.03	0.386HisHis: 0.386 ± 0.023	1.369HisIle: 1.369 ± 0.034	0.977HisLys: 0.977 ± 0.031	1.296HisLeu: 1.296 ± 0.035	0.492HisMet: 0.492 ± 0.021	0.757HisAsn: 0.757 ± 0.028	0.727HisPro: 0.727 ± 0.024	0.414HisGln: 0.414 ± 0.021	0.679HisArg: 0.679 ± 0.025	0.977HisSer: 0.977 ± 0.031	0.84HisThr: 0.84 ± 0.023	1.071HisVal: 1.071 ± 0.032	0.146HisTrp: 0.146 ± 0.012	0.718HisTyr: 0.718 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.874IleAla: 5.874 ± 0.089	1.266IleCys: 1.266 ± 0.035	5.042IleAsp: 5.042 ± 0.075	5.551IleGlu: 5.551 ± 0.071	3.31IlePhe: 3.31 ± 0.06	4.95IleGly: 4.95 ± 0.07	1.298IleHis: 1.298 ± 0.034	5.908IleIle: 5.908 ± 0.093	5.384IleLys: 5.384 ± 0.068	6.364IleLeu: 6.364 ± 0.089	2.115IleMet: 2.115 ± 0.051	3.707IleAsn: 3.707 ± 0.059	3.006IlePro: 3.006 ± 0.059	1.795IleGln: 1.795 ± 0.043	3.523IleArg: 3.523 ± 0.061	5.72IleSer: 5.72 ± 0.093	4.354IleThr: 4.354 ± 0.069	5.189IleVal: 5.189 ± 0.078	0.612IleTrp: 0.612 ± 0.026	3.172IleTyr: 3.172 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	5.443LysAla: 5.443 ± 0.085	0.874LysCys: 0.874 ± 0.028	4.636LysAsp: 4.636 ± 0.067	6.151LysGlu: 6.151 ± 0.079	2.586LysPhe: 2.586 ± 0.041	4.294LysGly: 4.294 ± 0.068	1.114LysHis: 1.114 ± 0.032	5.667LysIle: 5.667 ± 0.072	6.891LysLys: 6.891 ± 0.082	6.068LysLeu: 6.068 ± 0.075	2.178LysMet: 2.178 ± 0.041	4.598LysAsn: 4.598 ± 0.07	1.941LysPro: 1.941 ± 0.047	1.854LysGln: 1.854 ± 0.04	3.123LysArg: 3.123 ± 0.059	4.056LysSer: 4.056 ± 0.064	3.689LysThr: 3.689 ± 0.06	4.751LysVal: 4.751 ± 0.07	0.649LysTrp: 0.649 ± 0.022	3.8LysTyr: 3.8 ± 0.064	0.0LysXaa: 0.0 ± 0.0
Leu	6.067LeuAla: 6.067 ± 0.086	1.174LeuCys: 1.174 ± 0.035	5.098LeuAsp: 5.098 ± 0.073	5.686LeuGlu: 5.686 ± 0.077	3.522LeuPhe: 3.522 ± 0.06	5.305LeuGly: 5.305 ± 0.064	1.324LeuHis: 1.324 ± 0.037	6.224LeuIle: 6.224 ± 0.079	6.588LeuLys: 6.588 ± 0.079	7.095LeuLeu: 7.095 ± 0.094	2.536LeuMet: 2.536 ± 0.047	4.059LeuAsn: 4.059 ± 0.063	2.883LeuPro: 2.883 ± 0.054	2.166LeuGln: 2.166 ± 0.043	3.447LeuArg: 3.447 ± 0.059	6.155LeuSer: 6.155 ± 0.077	4.498LeuThr: 4.498 ± 0.069	4.91LeuVal: 4.91 ± 0.066	0.622LeuTrp: 0.622 ± 0.026	3.218LeuTyr: 3.218 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.249MetAla: 2.249 ± 0.043	0.355MetCys: 0.355 ± 0.018	1.87MetAsp: 1.87 ± 0.041	2.042MetGlu: 2.042 ± 0.046	1.088MetPhe: 1.088 ± 0.035	1.758MetGly: 1.758 ± 0.04	0.489MetHis: 0.489 ± 0.019	2.248MetIle: 2.248 ± 0.056	2.492MetLys: 2.492 ± 0.042	2.662MetLeu: 2.662 ± 0.045	0.917MetMet: 0.917 ± 0.032	1.748MetAsn: 1.748 ± 0.033	1.087MetPro: 1.087 ± 0.028	0.964MetGln: 0.964 ± 0.029	1.189MetArg: 1.189 ± 0.034	1.937MetSer: 1.937 ± 0.039	1.677MetThr: 1.677 ± 0.04	1.793MetVal: 1.793 ± 0.042	0.208MetTrp: 0.208 ± 0.013	0.964MetTyr: 0.964 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.807AsnAla: 3.807 ± 0.057	0.67AsnCys: 0.67 ± 0.024	3.038AsnAsp: 3.038 ± 0.06	3.726AsnGlu: 3.726 ± 0.061	1.982AsnPhe: 1.982 ± 0.047	4.031AsnGly: 4.031 ± 0.076	0.799AsnHis: 0.799 ± 0.028	4.144AsnIle: 4.144 ± 0.065	3.199AsnLys: 3.199 ± 0.056	3.767AsnLeu: 3.767 ± 0.055	1.476AsnMet: 1.476 ± 0.039	2.441AsnAsn: 2.441 ± 0.051	1.786AsnPro: 1.786 ± 0.043	1.121AsnGln: 1.121 ± 0.029	1.876AsnArg: 1.876 ± 0.042	3.18AsnSer: 3.18 ± 0.052	2.57AsnThr: 2.57 ± 0.052	3.503AsnVal: 3.503 ± 0.061	0.435AsnTrp: 0.435 ± 0.019	2.283AsnTyr: 2.283 ± 0.045	0.0AsnXaa: 0.0 ± 0.0
Pro	2.092ProAla: 2.092 ± 0.045	0.376ProCys: 0.376 ± 0.017	2.218ProAsp: 2.218 ± 0.048	3.181ProGlu: 3.181 ± 0.06	1.385ProPhe: 1.385 ± 0.035	1.889ProGly: 1.889 ± 0.045	0.494ProHis: 0.494 ± 0.019	2.027ProIle: 2.027 ± 0.043	1.837ProLys: 1.837 ± 0.039	2.464ProLeu: 2.464 ± 0.044	0.785ProMet: 0.785 ± 0.027	1.188ProAsn: 1.188 ± 0.038	0.537ProPro: 0.537 ± 0.026	0.825ProGln: 0.825 ± 0.026	0.888ProArg: 0.888 ± 0.03	1.841ProSer: 1.841 ± 0.046	1.501ProThr: 1.501 ± 0.038	2.492ProVal: 2.492 ± 0.047	0.285ProTrp: 0.285 ± 0.018	1.362ProTyr: 1.362 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	1.828GlnAla: 1.828 ± 0.045	0.283GlnCys: 0.283 ± 0.015	1.224GlnAsp: 1.224 ± 0.034	1.723GlnGlu: 1.723 ± 0.04	1.031GlnPhe: 1.031 ± 0.03	1.463GlnGly: 1.463 ± 0.038	0.406GlnHis: 0.406 ± 0.018	2.1GlnIle: 2.1 ± 0.039	2.153GlnLys: 2.153 ± 0.045	2.153GlnLeu: 2.153 ± 0.044	0.85GlnMet: 0.85 ± 0.026	1.377GlnAsn: 1.377 ± 0.034	0.658GlnPro: 0.658 ± 0.025	0.847GlnGln: 0.847 ± 0.033	1.114GlnArg: 1.114 ± 0.031	1.464GlnSer: 1.464 ± 0.039	1.309GlnThr: 1.309 ± 0.037	1.503GlnVal: 1.503 ± 0.04	0.237GlnTrp: 0.237 ± 0.015	1.1GlnTyr: 1.1 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.612ArgAla: 2.612 ± 0.044	0.536ArgCys: 0.536 ± 0.02	2.251ArgAsp: 2.251 ± 0.042	3.361ArgGlu: 3.361 ± 0.061	1.807ArgPhe: 1.807 ± 0.043	2.237ArgGly: 2.237 ± 0.045	0.721ArgHis: 0.721 ± 0.026	3.395ArgIle: 3.395 ± 0.066	3.381ArgLys: 3.381 ± 0.057	3.45ArgLeu: 3.45 ± 0.06	1.323ArgMet: 1.323 ± 0.035	2.218ArgAsn: 2.218 ± 0.047	1.134ArgPro: 1.134 ± 0.033	1.168ArgGln: 1.168 ± 0.034	2.029ArgArg: 2.029 ± 0.051	2.28ArgSer: 2.28 ± 0.045	1.981ArgThr: 1.981 ± 0.042	2.526ArgVal: 2.526 ± 0.043	0.364ArgTrp: 0.364 ± 0.02	1.866ArgTyr: 1.866 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.815SerAla: 4.815 ± 0.076	0.737SerCys: 0.737 ± 0.03	4.431SerAsp: 4.431 ± 0.06	5.039SerGlu: 5.039 ± 0.081	2.986SerPhe: 2.986 ± 0.056	5.164SerGly: 5.164 ± 0.075	0.978SerHis: 0.978 ± 0.031	4.768SerIle: 4.768 ± 0.066	4.286SerLys: 4.286 ± 0.07	5.17SerLeu: 5.17 ± 0.078	1.827SerMet: 1.827 ± 0.041	2.891SerAsn: 2.891 ± 0.062	1.675SerPro: 1.675 ± 0.039	1.562SerGln: 1.562 ± 0.039	2.409SerArg: 2.409 ± 0.048	4.361SerSer: 4.361 ± 0.086	3.124SerThr: 3.124 ± 0.063	4.533SerVal: 4.533 ± 0.073	0.565SerTrp: 0.565 ± 0.024	2.632SerTyr: 2.632 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.336ThrAla: 4.336 ± 0.067	0.589ThrCys: 0.589 ± 0.021	3.433ThrAsp: 3.433 ± 0.062	3.801ThrGlu: 3.801 ± 0.06	2.229ThrPhe: 2.229 ± 0.047	4.23ThrGly: 4.23 ± 0.072	0.792ThrHis: 0.792 ± 0.027	3.887ThrIle: 3.887 ± 0.058	3.034ThrLys: 3.034 ± 0.056	4.343ThrLeu: 4.343 ± 0.071	1.372ThrMet: 1.372 ± 0.03	2.08ThrAsn: 2.08 ± 0.047	1.886ThrPro: 1.886 ± 0.041	1.248ThrGln: 1.248 ± 0.037	1.805ThrArg: 1.805 ± 0.044	3.23ThrSer: 3.23 ± 0.053	2.735ThrThr: 2.735 ± 0.062	4.185ThrVal: 4.185 ± 0.069	0.429ThrTrp: 0.429 ± 0.018	2.13ThrTyr: 2.13 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	4.322ValAla: 4.322 ± 0.064	1.033ValCys: 1.033 ± 0.031	4.169ValAsp: 4.169 ± 0.066	4.619ValGlu: 4.619 ± 0.067	2.99ValPhe: 2.99 ± 0.052	4.145ValGly: 4.145 ± 0.071	0.977ValHis: 0.977 ± 0.033	5.436ValIle: 5.436 ± 0.073	5.02ValLys: 5.02 ± 0.069	5.824ValLeu: 5.824 ± 0.071	2.058ValMet: 2.058 ± 0.042	3.278ValAsn: 3.278 ± 0.052	2.299ValPro: 2.299 ± 0.048	1.555ValGln: 1.555 ± 0.035	2.841ValArg: 2.841 ± 0.048	4.911ValSer: 4.911 ± 0.078	3.884ValThr: 3.884 ± 0.061	4.738ValVal: 4.738 ± 0.066	0.548ValTrp: 0.548 ± 0.023	2.755ValTyr: 2.755 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.5TrpAla: 0.5 ± 0.021	0.162TrpCys: 0.162 ± 0.013	0.561TrpAsp: 0.561 ± 0.027	0.546TrpGlu: 0.546 ± 0.023	0.428TrpPhe: 0.428 ± 0.019	0.626TrpGly: 0.626 ± 0.028	0.186TrpHis: 0.186 ± 0.014	0.665TrpIle: 0.665 ± 0.026	0.704TrpLys: 0.704 ± 0.026	0.791TrpLeu: 0.791 ± 0.03	0.267TrpMet: 0.267 ± 0.015	0.583TrpAsn: 0.583 ± 0.023	0.199TrpPro: 0.199 ± 0.015	0.292TrpGln: 0.292 ± 0.018	0.341TrpArg: 0.341 ± 0.016	0.509TrpSer: 0.509 ± 0.023	0.455TrpThr: 0.455 ± 0.024	0.41TrpVal: 0.41 ± 0.022	0.107TrpTrp: 0.107 ± 0.01	0.4TrpTyr: 0.4 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.844TyrAla: 2.844 ± 0.059	0.642TyrCys: 0.642 ± 0.023	3.069TyrAsp: 3.069 ± 0.062	3.2TyrGlu: 3.2 ± 0.058	2.044TyrPhe: 2.044 ± 0.041	2.973TyrGly: 2.973 ± 0.061	0.785TyrHis: 0.785 ± 0.024	3.289TyrIle: 3.289 ± 0.061	2.832TyrLys: 2.832 ± 0.053	3.47TyrLeu: 3.47 ± 0.054	1.17TyrMet: 1.17 ± 0.034	2.102TyrAsn: 2.102 ± 0.044	1.362TyrPro: 1.362 ± 0.036	1.077TyrGln: 1.077 ± 0.03	1.948TyrArg: 1.948 ± 0.052	2.828TyrSer: 2.828 ± 0.051	2.331TyrThr: 2.331 ± 0.053	2.724TyrVal: 2.724 ± 0.049	0.402TyrTrp: 0.402 ± 0.02	2.103TyrTyr: 2.103 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3398 proteins (1154851 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---