Nocardia sp. SYP-A9097

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia; unclassified Nocardia

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7799 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I2FRJ3|A0A6I2FRJ3_9NOCA PH domain-containing protein OS=Nocardia sp. SYP-A9097 OX=2663237 GN=GFY24_02175 PE=4 SV=1
MM1 pKa = 7.29TLYY4 pKa = 10.89GVDD7 pKa = 3.18ISNWQAGISISEE19 pKa = 3.92IAAEE23 pKa = 4.15GFSWVEE29 pKa = 3.89AKK31 pKa = 10.7VSEE34 pKa = 4.09GDD36 pKa = 3.77YY37 pKa = 11.04LQDD40 pKa = 3.61PTWLGFLASAQAIDD54 pKa = 4.23LPIIGYY60 pKa = 9.17HH61 pKa = 5.72YY62 pKa = 11.58AMADD66 pKa = 3.69CAPAAQVRR74 pKa = 11.84TWLAGNGGANAMIDD88 pKa = 3.76FEE90 pKa = 5.17GGGGAIGDD98 pKa = 4.12FWALVQAFNDD108 pKa = 3.33AGVNVVLSYY117 pKa = 10.85LPQWYY122 pKa = 8.01WEE124 pKa = 4.31QIGSPDD130 pKa = 3.51LSQVPGLVALAYY142 pKa = 9.73PDD144 pKa = 4.16GSGDD148 pKa = 3.88ACDD151 pKa = 4.95LYY153 pKa = 11.37AAAGGDD159 pKa = 3.77SGEE162 pKa = 4.02GWTPYY167 pKa = 10.97GNAQPLIWQFSNQASVAGMSVDD189 pKa = 3.63ANAFRR194 pKa = 11.84GDD196 pKa = 3.37IDD198 pKa = 4.31EE199 pKa = 4.35LTALIGDD206 pKa = 4.51DD207 pKa = 3.9MTPQQAQQLQDD218 pKa = 2.23IWDD221 pKa = 3.73QLRR224 pKa = 11.84GPNGQGWPQLGHH236 pKa = 6.25NSEE239 pKa = 4.32GHH241 pKa = 6.0NLTPVDD247 pKa = 4.12ALAGLITTVNGLAADD262 pKa = 3.84VQTLKK267 pKa = 10.99GATTHH272 pKa = 6.05EE273 pKa = 4.34PRR275 pKa = 5.25

Molecular weight: 29.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I2FZ14|A0A6I2FZ14_9NOCA S-(Hydroxymethyl)mycothiol dehydrogenase OS=Nocardia sp. SYP-A9097 OX=2663237 GN=GFY24_28205 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.12GRR42 pKa = 11.84KK43 pKa = 9.05SLTAA47 pKa = 4.07

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7799

0

7799

2561633

24

14833

328.5

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.053 ± 0.039

0.757 ± 0.01

6.042 ± 0.025

5.512 ± 0.03

3.015 ± 0.019

8.785 ± 0.027

2.275 ± 0.015

4.391 ± 0.018

2.075 ± 0.022

10.174 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.948 ± 0.012

2.191 ± 0.016

5.823 ± 0.028

3.008 ± 0.017

7.511 ± 0.027

5.395 ± 0.019

6.252 ± 0.024

8.174 ± 0.032

1.516 ± 0.014

2.102 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski