Paracoccidioides brasiliensis (strain Pb18)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Onygenales incertae sedis; Paracoccidioides; Paracoccidioides brasiliensis

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8399 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C1G7U6|C1G7U6_PARBD Zn(2)-C6 fungal-type domain-containing protein OS=Paracoccidioides brasiliensis (strain Pb18) OX=502780 GN=PADG_03251 PE=4 SV=1
MM1 pKa = 8.13RR2 pKa = 11.84YY3 pKa = 9.98LKK5 pKa = 10.92GIDD8 pKa = 2.95ATYY11 pKa = 10.2FVICKK16 pKa = 10.09AEE18 pKa = 4.2HH19 pKa = 5.31EE20 pKa = 4.3QGMDD24 pKa = 3.5VVVDD28 pKa = 5.17DD29 pKa = 6.5DD30 pKa = 6.64DD31 pKa = 7.62DD32 pKa = 7.4DD33 pKa = 7.32DD34 pKa = 6.54DD35 pKa = 6.36DD36 pKa = 4.77EE37 pKa = 5.48YY38 pKa = 11.57GIFGINPIEE47 pKa = 4.47GQGHH51 pKa = 7.34LLNKK55 pKa = 10.03YY56 pKa = 9.4SALTASCDD64 pKa = 3.86FQGSNLSDD72 pKa = 3.57FEE74 pKa = 4.47YY75 pKa = 10.96AGTDD79 pKa = 3.59RR80 pKa = 11.84NCSS83 pKa = 3.26

Molecular weight: 9.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C1GF34|C1GF34_PARBD SMI1_KNR4 domain-containing protein OS=Paracoccidioides brasiliensis (strain Pb18) OX=502780 GN=PADG_05870 PE=3 SV=2
MM1 pKa = 7.87PSHH4 pKa = 5.85KK5 pKa = 9.4TFRR8 pKa = 11.84TKK10 pKa = 10.46QKK12 pKa = 9.92LAKK15 pKa = 8.75AQKK18 pKa = 9.19RR19 pKa = 11.84NRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 4.53

Molecular weight: 4.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8399

0

8399

3885900

31

5137

462.7

51.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.783 ± 0.023

1.218 ± 0.01

5.53 ± 0.018

6.202 ± 0.028

3.633 ± 0.016

6.72 ± 0.028

2.483 ± 0.011

5.112 ± 0.017

5.079 ± 0.023

8.842 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.009

4.009 ± 0.013

6.263 ± 0.029

4.112 ± 0.02

6.362 ± 0.022

8.913 ± 0.032

5.88 ± 0.018

5.868 ± 0.017

1.279 ± 0.008

2.624 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski