Mycobacterium asiaticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4763 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A3N7G4|A0A1A3N7G4_MYCAS Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase OS=Mycobacterium asiaticum OX=1790 GN=cobT PE=3 SV=1
MM1 pKa = 7.76AAPDD5 pKa = 4.25LMTAAAANAAGIGSSIGAANLAALSPTSTVLAAAADD41 pKa = 4.07EE42 pKa = 4.43VSAAIASLFANHH54 pKa = 6.93ALDD57 pKa = 3.72YY58 pKa = 9.83QTFSAQVAGSYY69 pKa = 8.09EE70 pKa = 4.1QFVRR74 pKa = 11.84TLSAASGAYY83 pKa = 9.3AAAEE87 pKa = 4.03AASAGPLQPVLDD99 pKa = 4.67VINAPTNFLLGRR111 pKa = 11.84PLIGDD116 pKa = 4.29GVNGVAGTGQAGGDD130 pKa = 3.94GGILWGNGGTGGSGGAGQAGGRR152 pKa = 11.84GGNAGLIGDD161 pKa = 5.01GGTGGTGGLRR171 pKa = 11.84GGVGGAGGTGGWLLGNGGAGGTGGFGGNLYY201 pKa = 10.99GGGTIPAGAGGIGGRR216 pKa = 11.84ALSLFGASGATGNVGTFRR234 pKa = 11.84NVTFVSAEE242 pKa = 4.04DD243 pKa = 3.43AAVWAMANPDD253 pKa = 4.02ANFLLIGTDD262 pKa = 3.43GTNLSRR268 pKa = 11.84ILADD272 pKa = 3.78AAGTPNFHH280 pKa = 7.6ALMEE284 pKa = 4.13QSVTSASTVIGHH296 pKa = 5.39TTVSNPSWTTIQTGVWSEE314 pKa = 4.17TAGVTNNIFSPWTYY328 pKa = 11.28DD329 pKa = 3.62DD330 pKa = 3.74WPTVYY335 pKa = 10.8NHH337 pKa = 7.15LEE339 pKa = 3.71NAYY342 pKa = 10.22GDD344 pKa = 4.17GVNTTVIADD353 pKa = 3.58WKK355 pKa = 10.53VITDD359 pKa = 3.57IAGAGSNPADD369 pKa = 4.09NITFVPQVAGDD380 pKa = 4.21TNWEE384 pKa = 3.99ATSNLVAANAIDD396 pKa = 5.21AIAAADD402 pKa = 3.91PDD404 pKa = 3.79KK405 pKa = 11.63GNLIFAYY412 pKa = 8.5FTGPDD417 pKa = 3.89VNAHH421 pKa = 6.67AYY423 pKa = 10.31GGDD426 pKa = 3.67SPQYY430 pKa = 9.74AAALRR435 pKa = 11.84TMDD438 pKa = 4.77ANLGSQTNGTGMLGAVAQWEE458 pKa = 4.12AANPGEE464 pKa = 4.37EE465 pKa = 3.99FSVLVVTDD473 pKa = 3.73HH474 pKa = 7.12GEE476 pKa = 3.9LGPAQFGGITHH487 pKa = 7.33GFQSPLEE494 pKa = 4.14TASFVIFDD502 pKa = 3.62QAFDD506 pKa = 3.71NSSDD510 pKa = 3.5GLINNSWQIVSTTPTILDD528 pKa = 3.33QFYY531 pKa = 10.57LPQPSYY537 pKa = 10.16MQGAPITSPVFDD549 pKa = 3.94GTYY552 pKa = 10.52VDD554 pKa = 4.48PGPNLFSALSADD566 pKa = 4.79FAAQGYY572 pKa = 8.63PDD574 pKa = 3.67VVTNVSLSARR584 pKa = 11.84TLAASVPYY592 pKa = 10.38LAFEE596 pKa = 5.14PINGFIHH603 pKa = 6.99SMPEE607 pKa = 3.89FLQLPVSWIGAGVYY621 pKa = 7.41QTLNIPAQMFVRR633 pKa = 11.84FTGVTGNEE641 pKa = 4.28IIPPILNPFISS652 pKa = 3.51

Molecular weight: 65.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A3NE44|A0A1A3NE44_MYCAS Uncharacterized protein OS=Mycobacterium asiaticum OX=1790 GN=A5636_02035 PE=4 SV=1
MM1 pKa = 7.5QIVLLVAGAVFIISTLAVTVRR22 pKa = 11.84RR23 pKa = 11.84LMKK26 pKa = 10.32AAPHH30 pKa = 5.61SKK32 pKa = 10.04ARR34 pKa = 11.84AAMWGTLGQLGAVTIFLVLALQRR57 pKa = 11.84PHH59 pKa = 7.03SGNIFSWLILIALALAAINVVATLLIRR86 pKa = 11.84WLSSGIKK93 pKa = 9.37PP94 pKa = 4.05

Molecular weight: 10.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4763

0

4763

1590993

29

5956

334.0

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.823 ± 0.051

0.804 ± 0.01

6.106 ± 0.033

5.219 ± 0.036

3.067 ± 0.028

9.32 ± 0.18

2.219 ± 0.018

4.247 ± 0.028

2.218 ± 0.026

9.782 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.034 ± 0.017

2.481 ± 0.038

5.808 ± 0.034

3.183 ± 0.022

7.139 ± 0.046

5.532 ± 0.023

5.894 ± 0.021

8.454 ± 0.036

1.502 ± 0.015

2.166 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski