Mannheimia phage vB_MhM_3927AP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3LQM0|A0A0M3LQM0_9CAUD Uncharacterized protein OS=Mannheimia phage vB_MhM_3927AP2 OX=1572750 GN=3927AP2_26 PE=4 SV=1
MM1 pKa = 7.75TDD3 pKa = 3.37YY4 pKa = 11.28QKK6 pKa = 11.61LLNALLNDD14 pKa = 3.85ADD16 pKa = 4.14EE17 pKa = 5.23DD18 pKa = 4.27CFAVYY23 pKa = 10.4GLRR26 pKa = 11.84ATTDD30 pKa = 2.99VHH32 pKa = 6.74QVGDD36 pKa = 3.68EE37 pKa = 4.21LGNSFVWVDD46 pKa = 3.4GEE48 pKa = 4.39KK49 pKa = 9.92TDD51 pKa = 4.3EE52 pKa = 4.31EE53 pKa = 4.9LDD55 pKa = 4.54GICTMGIQNADD66 pKa = 3.21EE67 pKa = 4.96AGLLNAIKK75 pKa = 10.69NLGRR79 pKa = 11.84DD80 pKa = 3.16ACKK83 pKa = 10.63KK84 pKa = 10.23FDD86 pKa = 3.58VEE88 pKa = 4.78FKK90 pKa = 10.93GFQSYY95 pKa = 10.61CGQNFILVKK104 pKa = 10.37GDD106 pKa = 3.49SARR109 pKa = 11.84GGEE112 pKa = 4.45DD113 pKa = 2.67KK114 pKa = 11.39GEE116 pKa = 4.23SIIRR120 pKa = 11.84NPIVVAVFNSS130 pKa = 3.3

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3LQ11|A0A0M3LQ11_9CAUD Major head subunit T OS=Mannheimia phage vB_MhM_3927AP2 OX=1572750 GN=3927AP2_32 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.3LIKK5 pKa = 10.07KK6 pKa = 9.52LRR8 pKa = 11.84QRR10 pKa = 11.84WQQWRR15 pKa = 11.84FYY17 pKa = 10.75RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84PDD22 pKa = 3.1LAEE25 pKa = 3.64LHH27 pKa = 5.88NVVRR31 pKa = 11.84AAVAQGNQHH40 pKa = 5.71PVGRR44 pKa = 11.84AKK46 pKa = 10.53RR47 pKa = 11.84GRR49 pKa = 11.84YY50 pKa = 8.68DD51 pKa = 2.94

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10540

48

759

210.8

23.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.776 ± 0.496

0.901 ± 0.142

5.655 ± 0.267

7.163 ± 0.264

3.643 ± 0.244

6.765 ± 0.251

1.736 ± 0.133

5.712 ± 0.274

7.457 ± 0.381

9.222 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.296 ± 0.166

5.228 ± 0.426

3.226 ± 0.222

4.744 ± 0.27

5.142 ± 0.295

5.911 ± 0.243

5.351 ± 0.31

6.945 ± 0.309

1.243 ± 0.16

2.884 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski