Chanos chanos (Milkfish) (Mugil chanos)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai;

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28996 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J2UPL6|A0A6J2UPL6_CHACN differentially expressed in FDCP 8 homolog isoform X1 OS=Chanos chanos OX=29144 GN=def8 PE=4 SV=1
MM1 pKa = 7.63AALPCAAVFCDD12 pKa = 4.55FTRR15 pKa = 11.84GSISHH20 pKa = 7.0LNSPVPSVPPCPVPIALEE38 pKa = 4.16SHH40 pKa = 6.8CPHH43 pKa = 6.9SGPTEE48 pKa = 4.21GDD50 pKa = 3.65CCGSCPLPSVPPITMPTVPTVDD72 pKa = 4.24LCHH75 pKa = 6.83DD76 pKa = 4.59ACPPTDD82 pKa = 3.27RR83 pKa = 11.84MEE85 pKa = 4.2VLGAQDD91 pKa = 3.59PAMEE95 pKa = 5.38EE96 pKa = 3.56IPLQKK101 pKa = 10.61KK102 pKa = 9.82GDD104 pKa = 3.82RR105 pKa = 11.84LPFQIFPDD113 pKa = 3.85PVEE116 pKa = 4.25VVLGPEE122 pKa = 4.0GTLNCVEE129 pKa = 4.14QEE131 pKa = 4.05KK132 pKa = 10.63EE133 pKa = 4.03RR134 pKa = 11.84LPSIVVEE141 pKa = 4.05PTEE144 pKa = 4.0VSEE147 pKa = 4.46VEE149 pKa = 4.2SGEE152 pKa = 4.45LRR154 pKa = 11.84WPPEE158 pKa = 3.72NMDD161 pKa = 3.64FEE163 pKa = 4.63EE164 pKa = 6.66DD165 pKa = 3.13EE166 pKa = 5.18DD167 pKa = 5.65LFLEE171 pKa = 4.55QCIPPANIADD181 pKa = 3.43WGEE184 pKa = 3.97EE185 pKa = 3.99EE186 pKa = 4.4EE187 pKa = 4.43EE188 pKa = 4.34EE189 pKa = 4.24EE190 pKa = 4.61EE191 pKa = 4.16EE192 pKa = 4.16TSVIINNQSSLLDD205 pKa = 3.72LQSDD209 pKa = 4.33DD210 pKa = 5.42FRR212 pKa = 11.84DD213 pKa = 4.03DD214 pKa = 3.67TPTLPPEE221 pKa = 4.36SASALNN227 pKa = 3.62

Molecular weight: 24.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J2UR46|A0A6J2UR46_CHACN zinc finger protein ZIC 3-like OS=Chanos chanos OX=29144 GN=LOC115805359 PE=3 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23048

5948

28996

17709472

31

31379

610.8

68.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.487 ± 0.012

2.152 ± 0.013

5.24 ± 0.011

7.103 ± 0.02

3.589 ± 0.01

6.36 ± 0.019

2.57 ± 0.008

4.464 ± 0.011

5.709 ± 0.018

9.423 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.006

3.881 ± 0.009

5.684 ± 0.019

4.732 ± 0.016

5.658 ± 0.012

8.513 ± 0.018

5.704 ± 0.015

6.405 ± 0.013

1.135 ± 0.005

2.803 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski