Amino acid dipepetide frequency for Cellulosimicrobium sp. MM

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.42AlaAla: 22.42 ± 0.317
0.881AlaCys: 0.881 ± 0.047
8.91AlaAsp: 8.91 ± 0.139
8.194AlaGlu: 8.194 ± 0.16
3.425AlaPhe: 3.425 ± 0.079
13.677AlaGly: 13.677 ± 0.195
2.617AlaHis: 2.617 ± 0.075
3.717AlaIle: 3.717 ± 0.099
2.256AlaLys: 2.256 ± 0.082
14.419AlaLeu: 14.419 ± 0.208
2.427AlaMet: 2.427 ± 0.067
1.87AlaAsn: 1.87 ± 0.058
8.172AlaPro: 8.172 ± 0.177
4.0AlaGln: 4.0 ± 0.081
12.237AlaArg: 12.237 ± 0.233
6.86AlaSer: 6.86 ± 0.124
8.406AlaThr: 8.406 ± 0.133
13.722AlaVal: 13.722 ± 0.18
2.219AlaTrp: 2.219 ± 0.066
2.307AlaTyr: 2.307 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.848CysAla: 0.848 ± 0.045
0.061CysCys: 0.061 ± 0.013
0.303CysAsp: 0.303 ± 0.024
0.305CysGlu: 0.305 ± 0.024
0.156CysPhe: 0.156 ± 0.017
0.701CysGly: 0.701 ± 0.038
0.123CysHis: 0.123 ± 0.014
0.106CysIle: 0.106 ± 0.017
0.046CysLys: 0.046 ± 0.011
0.422CysLeu: 0.422 ± 0.03
0.084CysMet: 0.084 ± 0.013
0.078CysAsn: 0.078 ± 0.012
0.453CysPro: 0.453 ± 0.031
0.151CysGln: 0.151 ± 0.017
0.424CysArg: 0.424 ± 0.032
0.498CysSer: 0.498 ± 0.037
0.392CysThr: 0.392 ± 0.028
0.526CysVal: 0.526 ± 0.027
0.108CysTrp: 0.108 ± 0.018
0.106CysTyr: 0.106 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
10.362AspAla: 10.362 ± 0.156
0.258AspCys: 0.258 ± 0.022
5.392AspAsp: 5.392 ± 0.129
4.358AspGlu: 4.358 ± 0.093
1.377AspPhe: 1.377 ± 0.053
7.124AspGly: 7.124 ± 0.119
1.223AspHis: 1.223 ± 0.049
1.229AspIle: 1.229 ± 0.05
0.801AspLys: 0.801 ± 0.047
6.843AspLeu: 6.843 ± 0.128
0.76AspMet: 0.76 ± 0.033
0.734AspAsn: 0.734 ± 0.04
4.868AspPro: 4.868 ± 0.101
1.39AspGln: 1.39 ± 0.054
4.877AspArg: 4.877 ± 0.099
1.942AspSer: 1.942 ± 0.064
2.632AspThr: 2.632 ± 0.076
7.592AspVal: 7.592 ± 0.121
0.888AspTrp: 0.888 ± 0.047
0.963AspTyr: 0.963 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
7.429GluAla: 7.429 ± 0.149
0.253GluCys: 0.253 ± 0.023
3.158GluAsp: 3.158 ± 0.079
3.029GluGlu: 3.029 ± 0.075
1.225GluPhe: 1.225 ± 0.052
4.423GluGly: 4.423 ± 0.091
1.741GluHis: 1.741 ± 0.059
2.362GluIle: 2.362 ± 0.071
1.214GluLys: 1.214 ± 0.06
6.221GluLeu: 6.221 ± 0.11
0.795GluMet: 0.795 ± 0.04
0.983GluAsn: 0.983 ± 0.049
3.498GluPro: 3.498 ± 0.091
2.054GluGln: 2.054 ± 0.06
5.622GluArg: 5.622 ± 0.109
2.429GluSer: 2.429 ± 0.075
2.95GluThr: 2.95 ± 0.071
5.382GluVal: 5.382 ± 0.11
0.68GluTrp: 0.68 ± 0.038
1.037GluTyr: 1.037 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.498PheAla: 3.498 ± 0.082
0.16PheCys: 0.16 ± 0.019
1.983PheAsp: 1.983 ± 0.068
1.431PheGlu: 1.431 ± 0.047
0.816PhePhe: 0.816 ± 0.043
2.528PheGly: 2.528 ± 0.071
0.457PheHis: 0.457 ± 0.03
0.582PheIle: 0.582 ± 0.035
0.407PheLys: 0.407 ± 0.031
2.437PheLeu: 2.437 ± 0.08
0.327PheMet: 0.327 ± 0.026
0.446PheAsn: 0.446 ± 0.028
1.189PhePro: 1.189 ± 0.047
0.61PheGln: 0.61 ± 0.035
1.623PheArg: 1.623 ± 0.06
1.279PheSer: 1.279 ± 0.054
1.758PheThr: 1.758 ± 0.057
2.609PheVal: 2.609 ± 0.074
0.411PheTrp: 0.411 ± 0.032
0.554PheTyr: 0.554 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
12.326GlyAla: 12.326 ± 0.188
0.626GlyCys: 0.626 ± 0.036
5.431GlyAsp: 5.431 ± 0.111
5.384GlyGlu: 5.384 ± 0.116
2.725GlyPhe: 2.725 ± 0.073
8.419GlyGly: 8.419 ± 0.182
1.836GlyHis: 1.836 ± 0.062
3.033GlyIle: 3.033 ± 0.075
1.965GlyLys: 1.965 ± 0.07
8.977GlyLeu: 8.977 ± 0.14
1.805GlyMet: 1.805 ± 0.06
1.366GlyAsn: 1.366 ± 0.053
5.184GlyPro: 5.184 ± 0.089
2.29GlyGln: 2.29 ± 0.074
7.524GlyArg: 7.524 ± 0.136
5.49GlySer: 5.49 ± 0.096
6.795GlyThr: 6.795 ± 0.126
8.815GlyVal: 8.815 ± 0.133
1.595GlyTrp: 1.595 ± 0.052
1.978GlyTyr: 1.978 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
2.608HisAla: 2.608 ± 0.075
0.13HisCys: 0.13 ± 0.017
1.539HisAsp: 1.539 ± 0.052
1.136HisGlu: 1.136 ± 0.042
0.44HisPhe: 0.44 ± 0.027
2.054HisGly: 2.054 ± 0.069
0.53HisHis: 0.53 ± 0.034
0.398HisIle: 0.398 ± 0.032
0.26HisLys: 0.26 ± 0.019
2.158HisLeu: 2.158 ± 0.064
0.249HisMet: 0.249 ± 0.021
0.279HisAsn: 0.279 ± 0.021
1.42HisPro: 1.42 ± 0.05
0.463HisGln: 0.463 ± 0.031
1.736HisArg: 1.736 ± 0.057
0.649HisSer: 0.649 ± 0.034
0.898HisThr: 0.898 ± 0.042
2.197HisVal: 2.197 ± 0.075
0.223HisTrp: 0.223 ± 0.019
0.379HisTyr: 0.379 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
3.825IleAla: 3.825 ± 0.094
0.152IleCys: 0.152 ± 0.016
2.201IleAsp: 2.201 ± 0.065
1.953IleGlu: 1.953 ± 0.065
0.762IlePhe: 0.762 ± 0.044
2.65IleGly: 2.65 ± 0.073
0.435IleHis: 0.435 ± 0.026
0.875IleIle: 0.875 ± 0.049
0.589IleLys: 0.589 ± 0.039
2.368IleLeu: 2.368 ± 0.065
0.42IleMet: 0.42 ± 0.027
0.556IleAsn: 0.556 ± 0.031
1.478IlePro: 1.478 ± 0.057
0.624IleGln: 0.624 ± 0.037
1.699IleArg: 1.699 ± 0.064
1.34IleSer: 1.34 ± 0.051
1.927IleThr: 1.927 ± 0.06
3.031IleVal: 3.031 ± 0.08
0.299IleTrp: 0.299 ± 0.024
0.474IleTyr: 0.474 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
2.163LysAla: 2.163 ± 0.082
0.054LysCys: 0.054 ± 0.011
0.985LysAsp: 0.985 ± 0.049
0.868LysGlu: 0.868 ± 0.047
0.394LysPhe: 0.394 ± 0.028
1.427LysGly: 1.427 ± 0.056
0.374LysHis: 0.374 ± 0.025
0.846LysIle: 0.846 ± 0.04
0.572LysLys: 0.572 ± 0.032
1.318LysLeu: 1.318 ± 0.055
0.32LysMet: 0.32 ± 0.026
0.426LysAsn: 0.426 ± 0.028
1.013LysPro: 1.013 ± 0.053
0.522LysGln: 0.522 ± 0.037
1.18LysArg: 1.18 ± 0.052
0.875LysSer: 0.875 ± 0.039
0.972LysThr: 0.972 ± 0.053
1.704LysVal: 1.704 ± 0.072
0.164LysTrp: 0.164 ± 0.018
0.32LysTyr: 0.32 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
15.976LeuAla: 15.976 ± 0.201
0.485LeuCys: 0.485 ± 0.033
7.451LeuAsp: 7.451 ± 0.113
5.024LeuGlu: 5.024 ± 0.101
2.254LeuPhe: 2.254 ± 0.085
9.451LeuGly: 9.451 ± 0.17
1.829LeuHis: 1.829 ± 0.066
2.005LeuIle: 2.005 ± 0.07
1.387LeuLys: 1.387 ± 0.059
10.012LeuLeu: 10.012 ± 0.169
1.351LeuMet: 1.351 ± 0.048
1.243LeuAsn: 1.243 ± 0.049
5.555LeuPro: 5.555 ± 0.118
1.979LeuGln: 1.979 ± 0.056
8.04LeuArg: 8.04 ± 0.142
4.593LeuSer: 4.593 ± 0.103
6.016LeuThr: 6.016 ± 0.104
11.124LeuVal: 11.124 ± 0.163
1.173LeuTrp: 1.173 ± 0.048
1.433LeuTyr: 1.433 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
1.838MetAla: 1.838 ± 0.061
0.084MetCys: 0.084 ± 0.013
0.829MetAsp: 0.829 ± 0.041
0.558MetGlu: 0.558 ± 0.031
0.413MetPhe: 0.413 ± 0.028
1.097MetGly: 1.097 ± 0.054
0.283MetHis: 0.283 ± 0.023
0.665MetIle: 0.665 ± 0.033
0.316MetLys: 0.316 ± 0.025
1.585MetLeu: 1.585 ± 0.055
0.236MetMet: 0.236 ± 0.02
0.305MetAsn: 0.305 ± 0.023
1.089MetPro: 1.089 ± 0.047
0.362MetGln: 0.362 ± 0.027
1.321MetArg: 1.321 ± 0.046
1.232MetSer: 1.232 ± 0.045
1.654MetThr: 1.654 ± 0.051
1.204MetVal: 1.204 ± 0.047
0.165MetTrp: 0.165 ± 0.017
0.208MetTyr: 0.208 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
1.985AsnAla: 1.985 ± 0.073
0.091AsnCys: 0.091 ± 0.015
0.987AsnAsp: 0.987 ± 0.047
0.751AsnGlu: 0.751 ± 0.044
0.368AsnPhe: 0.368 ± 0.022
1.351AsnGly: 1.351 ± 0.066
0.296AsnHis: 0.296 ± 0.022
0.543AsnIle: 0.543 ± 0.035
0.309AsnLys: 0.309 ± 0.029
1.623AsnLeu: 1.623 ± 0.064
0.201AsnMet: 0.201 ± 0.019
0.303AsnAsn: 0.303 ± 0.022
1.158AsnPro: 1.158 ± 0.054
0.474AsnGln: 0.474 ± 0.032
0.97AsnArg: 0.97 ± 0.044
0.604AsnSer: 0.604 ± 0.042
0.803AsnThr: 0.803 ± 0.042
1.561AsnVal: 1.561 ± 0.057
0.221AsnTrp: 0.221 ± 0.021
0.392AsnTyr: 0.392 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
8.873ProAla: 8.873 ± 0.184
0.243ProCys: 0.243 ± 0.023
4.738ProAsp: 4.738 ± 0.109
4.065ProGlu: 4.065 ± 0.1
1.504ProPhe: 1.504 ± 0.048
6.557ProGly: 6.557 ± 0.131
1.175ProHis: 1.175 ± 0.049
1.232ProIle: 1.232 ± 0.052
0.879ProLys: 0.879 ± 0.046
4.665ProLeu: 4.665 ± 0.098
0.719ProMet: 0.719 ± 0.041
0.805ProAsn: 0.805 ± 0.039
3.21ProPro: 3.21 ± 0.108
1.448ProGln: 1.448 ± 0.058
4.548ProArg: 4.548 ± 0.111
3.554ProSer: 3.554 ± 0.089
3.907ProThr: 3.907 ± 0.101
5.907ProVal: 5.907 ± 0.095
0.864ProTrp: 0.864 ± 0.051
1.019ProTyr: 1.019 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
3.293GlnAla: 3.293 ± 0.083
0.104GlnCys: 0.104 ± 0.013
1.446GlnAsp: 1.446 ± 0.054
1.336GlnGlu: 1.336 ± 0.053
0.624GlnPhe: 0.624 ± 0.037
2.05GlnGly: 2.05 ± 0.068
0.559GlnHis: 0.559 ± 0.033
0.937GlnIle: 0.937 ± 0.055
0.472GlnLys: 0.472 ± 0.029
2.366GlnLeu: 2.366 ± 0.071
0.444GlnMet: 0.444 ± 0.035
0.481GlnAsn: 0.481 ± 0.037
1.318GlnPro: 1.318 ± 0.053
0.957GlnGln: 0.957 ± 0.046
2.232GlnArg: 2.232 ± 0.062
1.162GlnSer: 1.162 ± 0.05
1.297GlnThr: 1.297 ± 0.053
2.883GlnVal: 2.883 ± 0.082
0.366GlnTrp: 0.366 ± 0.026
0.548GlnTyr: 0.548 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
11.484ArgAla: 11.484 ± 0.199
0.595ArgCys: 0.595 ± 0.036
4.853ArgAsp: 4.853 ± 0.109
4.68ArgGlu: 4.68 ± 0.098
2.026ArgPhe: 2.026 ± 0.06
6.405ArgGly: 6.405 ± 0.124
1.762ArgHis: 1.762 ± 0.058
2.411ArgIle: 2.411 ± 0.074
1.307ArgLys: 1.307 ± 0.051
7.932ArgLeu: 7.932 ± 0.157
1.567ArgMet: 1.567 ± 0.053
1.113ArgAsn: 1.113 ± 0.045
5.078ArgPro: 5.078 ± 0.122
1.948ArgGln: 1.948 ± 0.065
8.536ArgArg: 8.536 ± 0.211
4.685ArgSer: 4.685 ± 0.105
5.524ArgThr: 5.524 ± 0.111
7.462ArgVal: 7.462 ± 0.117
1.463ArgTrp: 1.463 ± 0.055
1.617ArgTyr: 1.617 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
6.953SerAla: 6.953 ± 0.134
0.385SerCys: 0.385 ± 0.03
2.569SerAsp: 2.569 ± 0.074
2.24SerGlu: 2.24 ± 0.064
1.459SerPhe: 1.459 ± 0.053
5.76SerGly: 5.76 ± 0.107
0.857SerHis: 0.857 ± 0.047
1.522SerIle: 1.522 ± 0.053
0.738SerLys: 0.738 ± 0.039
4.554SerLeu: 4.554 ± 0.096
1.05SerMet: 1.05 ± 0.047
0.812SerAsn: 0.812 ± 0.039
3.141SerPro: 3.141 ± 0.081
1.121SerGln: 1.121 ± 0.049
4.096SerArg: 4.096 ± 0.101
3.574SerSer: 3.574 ± 0.123
3.66SerThr: 3.66 ± 0.084
4.658SerVal: 4.658 ± 0.109
0.909SerTrp: 0.909 ± 0.041
0.994SerTyr: 0.994 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
8.18ThrAla: 8.18 ± 0.131
0.444ThrCys: 0.444 ± 0.033
3.743ThrAsp: 3.743 ± 0.098
3.122ThrGlu: 3.122 ± 0.076
1.931ThrPhe: 1.931 ± 0.059
6.247ThrGly: 6.247 ± 0.12
1.111ThrHis: 1.111 ± 0.047
2.08ThrIle: 2.08 ± 0.067
1.039ThrLys: 1.039 ± 0.041
5.962ThrLeu: 5.962 ± 0.114
0.866ThrMet: 0.866 ± 0.04
1.054ThrAsn: 1.054 ± 0.049
4.269ThrPro: 4.269 ± 0.094
1.31ThrGln: 1.31 ± 0.054
4.319ThrArg: 4.319 ± 0.089
3.844ThrSer: 3.844 ± 0.095
4.968ThrThr: 4.968 ± 0.134
6.533ThrVal: 6.533 ± 0.13
1.072ThrTrp: 1.072 ± 0.044
1.314ThrTyr: 1.314 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
14.651ValAla: 14.651 ± 0.185
0.677ValCys: 0.677 ± 0.036
6.866ValAsp: 6.866 ± 0.121
6.373ValGlu: 6.373 ± 0.12
2.21ValPhe: 2.21 ± 0.079
8.694ValGly: 8.694 ± 0.15
1.948ValHis: 1.948 ± 0.062
2.351ValIle: 2.351 ± 0.073
1.453ValLys: 1.453 ± 0.053
11.107ValLeu: 11.107 ± 0.181
1.385ValMet: 1.385 ± 0.052
1.455ValAsn: 1.455 ± 0.057
6.078ValPro: 6.078 ± 0.112
2.249ValGln: 2.249 ± 0.065
8.683ValArg: 8.683 ± 0.139
4.591ValSer: 4.591 ± 0.093
6.685ValThr: 6.685 ± 0.12
13.523ValVal: 13.523 ± 0.234
1.201ValTrp: 1.201 ± 0.046
1.524ValTyr: 1.524 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
1.725TrpAla: 1.725 ± 0.057
0.089TrpCys: 0.089 ± 0.014
0.861TrpAsp: 0.861 ± 0.044
0.658TrpGlu: 0.658 ± 0.039
0.491TrpPhe: 0.491 ± 0.031
1.11TrpGly: 1.11 ± 0.049
0.31TrpHis: 0.31 ± 0.025
0.427TrpIle: 0.427 ± 0.034
0.223TrpLys: 0.223 ± 0.021
1.465TrpLeu: 1.465 ± 0.056
0.24TrpMet: 0.24 ± 0.02
0.362TrpAsn: 0.362 ± 0.028
0.808TrpPro: 0.808 ± 0.036
0.465TrpGln: 0.465 ± 0.028
1.307TrpArg: 1.307 ± 0.054
0.998TrpSer: 0.998 ± 0.044
1.149TrpThr: 1.149 ± 0.046
1.281TrpVal: 1.281 ± 0.055
0.346TrpTrp: 0.346 ± 0.028
0.316TrpTyr: 0.316 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.303TyrAla: 2.303 ± 0.07
0.117TyrCys: 0.117 ± 0.015
1.372TyrAsp: 1.372 ± 0.06
1.02TyrGlu: 1.02 ± 0.045
0.504TyrPhe: 0.504 ± 0.032
1.838TyrGly: 1.838 ± 0.057
0.279TyrHis: 0.279 ± 0.024
0.338TyrIle: 0.338 ± 0.025
0.264TyrLys: 0.264 ± 0.022
1.95TyrLeu: 1.95 ± 0.066
0.197TyrMet: 0.197 ± 0.024
0.318TyrAsn: 0.318 ± 0.022
0.931TyrPro: 0.931 ± 0.047
0.461TyrGln: 0.461 ± 0.026
1.472TyrArg: 1.472 ± 0.059
0.851TyrSer: 0.851 ± 0.043
1.017TyrThr: 1.017 ± 0.046
1.989TyrVal: 1.989 ± 0.072
0.262TyrTrp: 0.262 ± 0.023
0.383TyrTyr: 0.383 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2081 proteins (538045 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski