Amino acid dipepetide frequency for Streptomyces actuosus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.508AlaAla: 22.508 ± 0.168	1.076AlaCys: 1.076 ± 0.025	8.629AlaAsp: 8.629 ± 0.084	9.06AlaGlu: 9.06 ± 0.096	3.528AlaPhe: 3.528 ± 0.046	13.527AlaGly: 13.527 ± 0.096	3.012AlaHis: 3.012 ± 0.037	3.165AlaIle: 3.165 ± 0.05	2.721AlaLys: 2.721 ± 0.051	15.224AlaLeu: 15.224 ± 0.113	2.378AlaMet: 2.378 ± 0.032	1.783AlaAsn: 1.783 ± 0.037	7.513AlaPro: 7.513 ± 0.094	3.839AlaGln: 3.839 ± 0.046	11.032AlaArg: 11.032 ± 0.088	5.841AlaSer: 5.841 ± 0.051	6.906AlaThr: 6.906 ± 0.057	12.687AlaVal: 12.687 ± 0.095	1.97AlaTrp: 1.97 ± 0.032	2.957AlaTyr: 2.957 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.115CysAla: 1.115 ± 0.024	0.104CysCys: 0.104 ± 0.007	0.451CysAsp: 0.451 ± 0.016	0.368CysGlu: 0.368 ± 0.013	0.208CysPhe: 0.208 ± 0.01	0.944CysGly: 0.944 ± 0.024	0.193CysHis: 0.193 ± 0.01	0.149CysIle: 0.149 ± 0.008	0.113CysLys: 0.113 ± 0.007	0.798CysLeu: 0.798 ± 0.02	0.119CysMet: 0.119 ± 0.007	0.129CysAsn: 0.129 ± 0.007	0.496CysPro: 0.496 ± 0.015	0.147CysGln: 0.147 ± 0.007	0.597CysArg: 0.597 ± 0.016	0.42CysSer: 0.42 ± 0.015	0.526CysThr: 0.526 ± 0.013	0.664CysVal: 0.664 ± 0.019	0.143CysTrp: 0.143 ± 0.007	0.151CysTyr: 0.151 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.927AspAla: 7.927 ± 0.064	0.419AspCys: 0.419 ± 0.013	3.66AspAsp: 3.66 ± 0.045	3.815AspGlu: 3.815 ± 0.051	1.606AspPhe: 1.606 ± 0.025	6.562AspGly: 6.562 ± 0.057	1.433AspHis: 1.433 ± 0.028	1.808AspIle: 1.808 ± 0.031	1.219AspLys: 1.219 ± 0.025	6.323AspLeu: 6.323 ± 0.066	0.769AspMet: 0.769 ± 0.019	0.923AspAsn: 0.923 ± 0.021	4.587AspPro: 4.587 ± 0.054	1.453AspGln: 1.453 ± 0.027	5.052AspArg: 5.052 ± 0.054	2.325AspSer: 2.325 ± 0.033	3.329AspThr: 3.329 ± 0.038	4.729AspVal: 4.729 ± 0.046	1.056AspTrp: 1.056 ± 0.02	1.116AspTyr: 1.116 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.584GluAla: 7.584 ± 0.084	0.35GluCys: 0.35 ± 0.012	2.897GluAsp: 2.897 ± 0.039	3.818GluGlu: 3.818 ± 0.058	1.425GluPhe: 1.425 ± 0.027	4.324GluGly: 4.324 ± 0.046	1.47GluHis: 1.47 ± 0.03	2.155GluIle: 2.155 ± 0.036	1.391GluLys: 1.391 ± 0.028	6.593GluLeu: 6.593 ± 0.068	0.845GluMet: 0.845 ± 0.02	0.939GluAsn: 0.939 ± 0.022	3.37GluPro: 3.37 ± 0.042	2.289GluGln: 2.289 ± 0.035	5.593GluArg: 5.593 ± 0.054	2.271GluSer: 2.271 ± 0.03	2.861GluThr: 2.861 ± 0.037	4.408GluVal: 4.408 ± 0.053	0.757GluTrp: 0.757 ± 0.021	1.107GluTyr: 1.107 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.684PheAla: 3.684 ± 0.043	0.248PheCys: 0.248 ± 0.011	1.905PheAsp: 1.905 ± 0.031	1.368PheGlu: 1.368 ± 0.026	0.887PhePhe: 0.887 ± 0.024	2.896PheGly: 2.896 ± 0.044	0.617PheHis: 0.617 ± 0.017	0.607PheIle: 0.607 ± 0.016	0.489PheLys: 0.489 ± 0.019	2.649PheLeu: 2.649 ± 0.043	0.383PheMet: 0.383 ± 0.013	0.521PheAsn: 0.521 ± 0.013	1.367PhePro: 1.367 ± 0.027	0.695PheGln: 0.695 ± 0.017	1.894PheArg: 1.894 ± 0.033	1.362PheSer: 1.362 ± 0.023	2.052PheThr: 2.052 ± 0.03	2.253PheVal: 2.253 ± 0.035	0.416PheTrp: 0.416 ± 0.012	0.603PheTyr: 0.603 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.372GlyAla: 11.372 ± 0.081	0.829GlyCys: 0.829 ± 0.02	5.219GlyAsp: 5.219 ± 0.041	5.013GlyGlu: 5.013 ± 0.054	2.872GlyPhe: 2.872 ± 0.043	8.908GlyGly: 8.908 ± 0.092	2.353GlyHis: 2.353 ± 0.033	3.272GlyIle: 3.272 ± 0.041	2.284GlyLys: 2.284 ± 0.037	9.592GlyLeu: 9.592 ± 0.078	1.906GlyMet: 1.906 ± 0.029	1.751GlyAsn: 1.751 ± 0.034	5.283GlyPro: 5.283 ± 0.054	2.677GlyGln: 2.677 ± 0.037	8.106GlyArg: 8.106 ± 0.065	5.087GlySer: 5.087 ± 0.054	6.736GlyThr: 6.736 ± 0.058	7.618GlyVal: 7.618 ± 0.07	1.772GlyTrp: 1.772 ± 0.029	2.321GlyTyr: 2.321 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.81HisAla: 2.81 ± 0.037	0.211HisCys: 0.211 ± 0.01	1.365HisAsp: 1.365 ± 0.025	1.203HisGlu: 1.203 ± 0.026	0.642HisPhe: 0.642 ± 0.017	2.458HisGly: 2.458 ± 0.038	0.687HisHis: 0.687 ± 0.02	0.667HisIle: 0.667 ± 0.018	0.359HisLys: 0.359 ± 0.012	2.416HisLeu: 2.416 ± 0.032	0.315HisMet: 0.315 ± 0.011	0.359HisAsn: 0.359 ± 0.013	1.912HisPro: 1.912 ± 0.033	0.597HisGln: 0.597 ± 0.016	2.162HisArg: 2.162 ± 0.03	0.936HisSer: 0.936 ± 0.02	1.363HisThr: 1.363 ± 0.023	1.711HisVal: 1.711 ± 0.026	0.395HisTrp: 0.395 ± 0.015	0.492HisTyr: 0.492 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.524IleAla: 4.524 ± 0.06	0.253IleCys: 0.253 ± 0.011	2.094IleAsp: 2.094 ± 0.029	1.824IleGlu: 1.824 ± 0.031	0.622IlePhe: 0.622 ± 0.018	3.279IleGly: 3.279 ± 0.042	0.533IleHis: 0.533 ± 0.014	0.753IleIle: 0.753 ± 0.022	0.678IleLys: 0.678 ± 0.018	2.251IleLeu: 2.251 ± 0.031	0.416IleMet: 0.416 ± 0.014	0.639IleAsn: 0.639 ± 0.018	1.639IlePro: 1.639 ± 0.031	0.686IleGln: 0.686 ± 0.018	2.136IleArg: 2.136 ± 0.033	1.414IleSer: 1.414 ± 0.027	2.018IleThr: 2.018 ± 0.03	2.628IleVal: 2.628 ± 0.038	0.358IleTrp: 0.358 ± 0.013	0.498IleTyr: 0.498 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.91LysAla: 2.91 ± 0.048	0.103LysCys: 0.103 ± 0.007	1.358LysAsp: 1.358 ± 0.029	1.19LysGlu: 1.19 ± 0.024	0.437LysPhe: 0.437 ± 0.015	1.789LysGly: 1.789 ± 0.036	0.4LysHis: 0.4 ± 0.013	0.831LysIle: 0.831 ± 0.021	0.863LysLys: 0.863 ± 0.031	1.935LysLeu: 1.935 ± 0.035	0.369LysMet: 0.369 ± 0.013	0.511LysAsn: 0.511 ± 0.018	1.278LysPro: 1.278 ± 0.026	0.688LysGln: 0.688 ± 0.021	1.392LysArg: 1.392 ± 0.027	1.051LysSer: 1.051 ± 0.024	1.235LysThr: 1.235 ± 0.027	1.865LysVal: 1.865 ± 0.036	0.268LysTrp: 0.268 ± 0.011	0.44LysTyr: 0.44 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.626LeuAla: 15.626 ± 0.114	0.873LeuCys: 0.873 ± 0.019	6.765LeuAsp: 6.765 ± 0.064	4.612LeuGlu: 4.612 ± 0.049	2.598LeuPhe: 2.598 ± 0.037	9.374LeuGly: 9.374 ± 0.076	2.334LeuHis: 2.334 ± 0.036	2.972LeuIle: 2.972 ± 0.045	2.112LeuLys: 2.112 ± 0.037	11.804LeuLeu: 11.804 ± 0.096	1.564LeuMet: 1.564 ± 0.028	1.6LeuAsn: 1.6 ± 0.028	6.654LeuPro: 6.654 ± 0.059	2.216LeuGln: 2.216 ± 0.028	8.922LeuArg: 8.922 ± 0.081	4.968LeuSer: 4.968 ± 0.049	7.095LeuThr: 7.095 ± 0.061	8.985LeuVal: 8.985 ± 0.088	1.327LeuTrp: 1.327 ± 0.027	1.921LeuTyr: 1.921 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.158MetAla: 2.158 ± 0.03	0.129MetCys: 0.129 ± 0.007	0.836MetAsp: 0.836 ± 0.018	0.689MetGlu: 0.689 ± 0.017	0.428MetPhe: 0.428 ± 0.014	1.237MetGly: 1.237 ± 0.025	0.324MetHis: 0.324 ± 0.012	0.595MetIle: 0.595 ± 0.018	0.408MetLys: 0.408 ± 0.014	1.567MetLeu: 1.567 ± 0.031	0.265MetMet: 0.265 ± 0.013	0.416MetAsn: 0.416 ± 0.014	1.081MetPro: 1.081 ± 0.02	0.437MetGln: 0.437 ± 0.013	1.378MetArg: 1.378 ± 0.025	1.258MetSer: 1.258 ± 0.024	1.505MetThr: 1.505 ± 0.029	1.183MetVal: 1.183 ± 0.025	0.208MetTrp: 0.208 ± 0.009	0.312MetTyr: 0.312 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.096AsnAla: 2.096 ± 0.036	0.156AsnCys: 0.156 ± 0.008	0.913AsnAsp: 0.913 ± 0.021	0.757AsnGlu: 0.757 ± 0.022	0.47AsnPhe: 0.47 ± 0.015	1.827AsnGly: 1.827 ± 0.036	0.385AsnHis: 0.385 ± 0.013	0.602AsnIle: 0.602 ± 0.018	0.385AsnLys: 0.385 ± 0.014	1.548AsnLeu: 1.548 ± 0.029	0.287AsnMet: 0.287 ± 0.01	0.415AsnAsn: 0.415 ± 0.015	1.284AsnPro: 1.284 ± 0.027	0.469AsnGln: 0.469 ± 0.017	1.195AsnArg: 1.195 ± 0.024	0.863AsnSer: 0.863 ± 0.021	1.088AsnThr: 1.088 ± 0.023	1.356AsnVal: 1.356 ± 0.025	0.285AsnTrp: 0.285 ± 0.013	0.376AsnTyr: 0.376 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.301ProAla: 9.301 ± 0.095	0.358ProCys: 0.358 ± 0.011	4.59ProAsp: 4.59 ± 0.048	4.404ProGlu: 4.404 ± 0.048	1.527ProPhe: 1.527 ± 0.026	6.907ProGly: 6.907 ± 0.079	1.49ProHis: 1.49 ± 0.028	1.104ProIle: 1.104 ± 0.023	1.132ProLys: 1.132 ± 0.024	5.512ProLeu: 5.512 ± 0.047	0.916ProMet: 0.916 ± 0.02	0.87ProAsn: 0.87 ± 0.023	3.797ProPro: 3.797 ± 0.061	1.808ProGln: 1.808 ± 0.041	4.227ProArg: 4.227 ± 0.05	3.08ProSer: 3.08 ± 0.039	3.145ProThr: 3.145 ± 0.052	5.58ProVal: 5.58 ± 0.048	0.911ProTrp: 0.911 ± 0.018	1.521ProTyr: 1.521 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.795GlnAla: 3.795 ± 0.048	0.169GlnCys: 0.169 ± 0.008	1.399GlnAsp: 1.399 ± 0.026	1.465GlnGlu: 1.465 ± 0.027	0.645GlnPhe: 0.645 ± 0.018	2.35GlnGly: 2.35 ± 0.032	0.634GlnHis: 0.634 ± 0.016	1.031GlnIle: 1.031 ± 0.022	0.619GlnLys: 0.619 ± 0.018	2.982GlnLeu: 2.982 ± 0.034	0.474GlnMet: 0.474 ± 0.013	0.466GlnAsn: 0.466 ± 0.017	1.717GlnPro: 1.717 ± 0.041	1.301GlnGln: 1.301 ± 0.032	2.379GlnArg: 2.379 ± 0.034	1.2GlnSer: 1.2 ± 0.025	1.372GlnThr: 1.372 ± 0.022	2.371GlnVal: 2.371 ± 0.032	0.468GlnTrp: 0.468 ± 0.014	0.622GlnTyr: 0.622 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	10.594ArgAla: 10.594 ± 0.086	0.579ArgCys: 0.579 ± 0.016	4.333ArgAsp: 4.333 ± 0.05	4.89ArgGlu: 4.89 ± 0.056	2.345ArgPhe: 2.345 ± 0.035	5.984ArgGly: 5.984 ± 0.057	2.233ArgHis: 2.233 ± 0.033	3.158ArgIle: 3.158 ± 0.04	1.599ArgLys: 1.599 ± 0.03	9.205ArgLeu: 9.205 ± 0.081	1.685ArgMet: 1.685 ± 0.025	1.263ArgAsn: 1.263 ± 0.023	5.38ArgPro: 5.38 ± 0.064	2.378ArgGln: 2.378 ± 0.037	8.171ArgArg: 8.171 ± 0.089	3.763ArgSer: 3.763 ± 0.04	5.598ArgThr: 5.598 ± 0.053	6.036ArgVal: 6.036 ± 0.049	1.429ArgTrp: 1.429 ± 0.025	1.859ArgTyr: 1.859 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.481SerAla: 6.481 ± 0.066	0.412SerCys: 0.412 ± 0.014	2.483SerAsp: 2.483 ± 0.034	2.205SerGlu: 2.205 ± 0.031	1.412SerPhe: 1.412 ± 0.029	5.759SerGly: 5.759 ± 0.054	0.949SerHis: 0.949 ± 0.021	1.215SerIle: 1.215 ± 0.025	0.981SerLys: 0.981 ± 0.026	4.55SerLeu: 4.55 ± 0.046	0.98SerMet: 0.98 ± 0.02	0.801SerAsn: 0.801 ± 0.018	3.102SerPro: 3.102 ± 0.041	1.116SerGln: 1.116 ± 0.025	3.448SerArg: 3.448 ± 0.037	2.585SerSer: 2.585 ± 0.041	2.839SerThr: 2.839 ± 0.04	4.135SerVal: 4.135 ± 0.048	0.862SerTrp: 0.862 ± 0.019	1.233SerTyr: 1.233 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.29ThrAla: 9.29 ± 0.075	0.426ThrCys: 0.426 ± 0.014	3.728ThrAsp: 3.728 ± 0.045	3.13ThrGlu: 3.13 ± 0.04	1.636ThrPhe: 1.636 ± 0.03	6.924ThrGly: 6.924 ± 0.073	1.21ThrHis: 1.21 ± 0.022	1.543ThrIle: 1.543 ± 0.03	1.107ThrLys: 1.107 ± 0.026	5.818ThrLeu: 5.818 ± 0.056	0.867ThrMet: 0.867 ± 0.02	0.926ThrAsn: 0.926 ± 0.021	4.37ThrPro: 4.37 ± 0.058	1.315ThrGln: 1.315 ± 0.024	4.047ThrArg: 4.047 ± 0.038	3.036ThrSer: 3.036 ± 0.04	3.851ThrThr: 3.851 ± 0.065	6.17ThrVal: 6.17 ± 0.051	0.954ThrTrp: 0.954 ± 0.019	1.417ThrTyr: 1.417 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	10.904ValAla: 10.904 ± 0.085	0.791ValCys: 0.791 ± 0.02	4.935ValAsp: 4.935 ± 0.053	4.626ValGlu: 4.626 ± 0.051	2.467ValPhe: 2.467 ± 0.037	6.368ValGly: 6.368 ± 0.064	2.026ValHis: 2.026 ± 0.03	2.603ValIle: 2.603 ± 0.041	1.69ValLys: 1.69 ± 0.029	9.626ValLeu: 9.626 ± 0.079	1.368ValMet: 1.368 ± 0.027	1.607ValAsn: 1.607 ± 0.028	5.486ValPro: 5.486 ± 0.057	2.1ValGln: 2.1 ± 0.027	7.503ValArg: 7.503 ± 0.074	4.208ValSer: 4.208 ± 0.047	5.885ValThr: 5.885 ± 0.058	8.113ValVal: 8.113 ± 0.085	1.234ValTrp: 1.234 ± 0.026	1.678ValTyr: 1.678 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.76TrpAla: 1.76 ± 0.03	0.158TrpCys: 0.158 ± 0.009	0.859TrpAsp: 0.859 ± 0.019	0.753TrpGlu: 0.753 ± 0.019	0.508TrpPhe: 0.508 ± 0.014	1.09TrpGly: 1.09 ± 0.022	0.375TrpHis: 0.375 ± 0.013	0.549TrpIle: 0.549 ± 0.016	0.358TrpLys: 0.358 ± 0.014	1.827TrpLeu: 1.827 ± 0.032	0.263TrpMet: 0.263 ± 0.01	0.418TrpAsn: 0.418 ± 0.014	0.813TrpPro: 0.813 ± 0.02	0.637TrpGln: 0.637 ± 0.015	1.372TrpArg: 1.372 ± 0.026	0.932TrpSer: 0.932 ± 0.022	1.104TrpThr: 1.104 ± 0.027	1.016TrpVal: 1.016 ± 0.023	0.371TrpTrp: 0.371 ± 0.014	0.379TrpTyr: 0.379 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.917TyrAla: 2.917 ± 0.035	0.19TyrCys: 0.19 ± 0.009	1.689TyrAsp: 1.689 ± 0.032	1.257TyrGlu: 1.257 ± 0.026	0.629TyrPhe: 0.629 ± 0.017	2.467TyrGly: 2.467 ± 0.038	0.403TyrHis: 0.403 ± 0.014	0.464TyrIle: 0.464 ± 0.011	0.406TyrLys: 0.406 ± 0.013	2.101TyrLeu: 2.101 ± 0.033	0.246TyrMet: 0.246 ± 0.01	0.419TyrAsn: 0.419 ± 0.014	1.052TyrPro: 1.052 ± 0.025	0.611TyrGln: 0.611 ± 0.018	1.878TyrArg: 1.878 ± 0.03	0.949TyrSer: 0.949 ± 0.022	1.258TyrThr: 1.258 ± 0.031	1.709TyrVal: 1.709 ± 0.029	0.358TyrTrp: 0.358 ± 0.011	0.474TyrTyr: 0.474 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7068 proteins (2320790 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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