Amino acid dipepetide frequency for Paenibacillaceae bacterium GAS479

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.282AlaAla: 11.282 ± 0.154	0.698AlaCys: 0.698 ± 0.02	4.539AlaAsp: 4.539 ± 0.061	6.237AlaGlu: 6.237 ± 0.085	3.26AlaPhe: 3.26 ± 0.047	8.074AlaGly: 8.074 ± 0.094	1.363AlaHis: 1.363 ± 0.038	5.139AlaIle: 5.139 ± 0.059	3.894AlaLys: 3.894 ± 0.059	8.773AlaLeu: 8.773 ± 0.092	2.402AlaMet: 2.402 ± 0.035	2.604AlaAsn: 2.604 ± 0.046	3.388AlaPro: 3.388 ± 0.125	2.805AlaGln: 2.805 ± 0.041	4.232AlaArg: 4.232 ± 0.073	6.197AlaSer: 6.197 ± 0.078	4.139AlaThr: 4.139 ± 0.102	6.923AlaVal: 6.923 ± 0.085	1.065AlaTrp: 1.065 ± 0.026	2.535AlaTyr: 2.535 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.473CysAla: 0.473 ± 0.019	0.099CysCys: 0.099 ± 0.009	0.336CysAsp: 0.336 ± 0.014	0.401CysGlu: 0.401 ± 0.016	0.279CysPhe: 0.279 ± 0.013	0.743CysGly: 0.743 ± 0.026	0.163CysHis: 0.163 ± 0.011	0.409CysIle: 0.409 ± 0.017	0.247CysLys: 0.247 ± 0.015	0.712CysLeu: 0.712 ± 0.021	0.178CysMet: 0.178 ± 0.011	0.235CysAsn: 0.235 ± 0.011	0.343CysPro: 0.343 ± 0.014	0.193CysGln: 0.193 ± 0.011	0.482CysArg: 0.482 ± 0.021	0.561CysSer: 0.561 ± 0.02	0.362CysThr: 0.362 ± 0.015	0.426CysVal: 0.426 ± 0.017	0.098CysTrp: 0.098 ± 0.009	0.254CysTyr: 0.254 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.149AspAla: 4.149 ± 0.063	0.354AspCys: 0.354 ± 0.017	2.315AspAsp: 2.315 ± 0.051	3.687AspGlu: 3.687 ± 0.047	1.979AspPhe: 1.979 ± 0.043	4.308AspGly: 4.308 ± 0.064	0.842AspHis: 0.842 ± 0.03	3.084AspIle: 3.084 ± 0.051	2.598AspLys: 2.598 ± 0.044	4.609AspLeu: 4.609 ± 0.065	1.236AspMet: 1.236 ± 0.034	1.859AspAsn: 1.859 ± 0.042	2.366AspPro: 2.366 ± 0.045	1.623AspGln: 1.623 ± 0.037	2.974AspArg: 2.974 ± 0.046	3.156AspSer: 3.156 ± 0.047	2.424AspThr: 2.424 ± 0.041	3.191AspVal: 3.191 ± 0.053	0.834AspTrp: 0.834 ± 0.024	2.015AspTyr: 2.015 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	6.644GluAla: 6.644 ± 0.089	0.323GluCys: 0.323 ± 0.014	2.894GluAsp: 2.894 ± 0.048	5.238GluGlu: 5.238 ± 0.087	2.136GluPhe: 2.136 ± 0.038	4.609GluGly: 4.609 ± 0.057	1.334GluHis: 1.334 ± 0.033	3.898GluIle: 3.898 ± 0.062	3.565GluLys: 3.565 ± 0.058	8.089GluLeu: 8.089 ± 0.099	1.875GluMet: 1.875 ± 0.038	2.226GluAsn: 2.226 ± 0.043	2.683GluPro: 2.683 ± 0.086	3.503GluGln: 3.503 ± 0.055	4.293GluArg: 4.293 ± 0.087	3.776GluSer: 3.776 ± 0.053	3.221GluThr: 3.221 ± 0.047	4.117GluVal: 4.117 ± 0.057	0.935GluTrp: 0.935 ± 0.026	1.666GluTyr: 1.666 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.14PheAla: 3.14 ± 0.042	0.313PheCys: 0.313 ± 0.017	2.105PheAsp: 2.105 ± 0.04	2.244PheGlu: 2.244 ± 0.044	1.711PhePhe: 1.711 ± 0.039	3.05PheGly: 3.05 ± 0.048	0.792PheHis: 0.792 ± 0.025	2.685PheIle: 2.685 ± 0.042	1.814PheLys: 1.814 ± 0.036	3.677PheLeu: 3.677 ± 0.058	1.034PheMet: 1.034 ± 0.024	1.612PheAsn: 1.612 ± 0.039	1.623PhePro: 1.623 ± 0.038	1.438PheGln: 1.438 ± 0.03	2.162PheArg: 2.162 ± 0.039	2.825PheSer: 2.825 ± 0.043	2.34PheThr: 2.34 ± 0.046	2.69PheVal: 2.69 ± 0.041	0.506PheTrp: 0.506 ± 0.02	1.41PheTyr: 1.41 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	6.845GlyAla: 6.845 ± 0.112	0.717GlyCys: 0.717 ± 0.022	3.671GlyAsp: 3.671 ± 0.053	4.723GlyGlu: 4.723 ± 0.061	3.227GlyPhe: 3.227 ± 0.045	6.494GlyGly: 6.494 ± 0.091	1.341GlyHis: 1.341 ± 0.03	5.516GlyIle: 5.516 ± 0.078	4.438GlyLys: 4.438 ± 0.066	7.737GlyLeu: 7.737 ± 0.079	2.5GlyMet: 2.5 ± 0.042	2.946GlyAsn: 2.946 ± 0.066	2.438GlyPro: 2.438 ± 0.068	2.78GlyGln: 2.78 ± 0.044	4.202GlyArg: 4.202 ± 0.062	5.835GlySer: 5.835 ± 0.075	4.667GlyThr: 4.667 ± 0.064	5.396GlyVal: 5.396 ± 0.077	1.236GlyTrp: 1.236 ± 0.028	2.877GlyTyr: 2.877 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.482HisAla: 1.482 ± 0.038	0.176HisCys: 0.176 ± 0.01	0.886HisAsp: 0.886 ± 0.026	1.186HisGlu: 1.186 ± 0.031	0.788HisPhe: 0.788 ± 0.028	1.491HisGly: 1.491 ± 0.032	0.483HisHis: 0.483 ± 0.016	1.03HisIle: 1.03 ± 0.028	0.735HisLys: 0.735 ± 0.024	1.802HisLeu: 1.802 ± 0.034	0.486HisMet: 0.486 ± 0.019	0.612HisAsn: 0.612 ± 0.019	1.126HisPro: 1.126 ± 0.03	0.623HisGln: 0.623 ± 0.017	1.057HisArg: 1.057 ± 0.028	1.209HisSer: 1.209 ± 0.031	0.903HisThr: 0.903 ± 0.024	1.168HisVal: 1.168 ± 0.029	0.25HisTrp: 0.25 ± 0.013	0.724HisTyr: 0.724 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.625IleAla: 5.625 ± 0.066	0.504IleCys: 0.504 ± 0.019	3.232IleAsp: 3.232 ± 0.052	3.81IleGlu: 3.81 ± 0.061	2.136IlePhe: 2.136 ± 0.048	5.295IleGly: 5.295 ± 0.065	1.218IleHis: 1.218 ± 0.029	3.799IleIle: 3.799 ± 0.055	2.816IleLys: 2.816 ± 0.057	5.332IleLeu: 5.332 ± 0.068	1.572IleMet: 1.572 ± 0.036	2.254IleAsn: 2.254 ± 0.046	2.96IlePro: 2.96 ± 0.052	2.192IleGln: 2.192 ± 0.034	3.91IleArg: 3.91 ± 0.054	4.193IleSer: 4.193 ± 0.06	3.408IleThr: 3.408 ± 0.056	4.764IleVal: 4.764 ± 0.063	0.665IleTrp: 0.665 ± 0.023	1.826IleTyr: 1.826 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	4.254LysAla: 4.254 ± 0.061	0.202LysCys: 0.202 ± 0.011	2.54LysAsp: 2.54 ± 0.048	3.715LysGlu: 3.715 ± 0.065	1.434LysPhe: 1.434 ± 0.035	3.583LysGly: 3.583 ± 0.056	0.884LysHis: 0.884 ± 0.022	2.522LysIle: 2.522 ± 0.05	2.689LysLys: 2.689 ± 0.055	5.576LysLeu: 5.576 ± 0.071	1.326LysMet: 1.326 ± 0.029	1.859LysAsn: 1.859 ± 0.039	2.372LysPro: 2.372 ± 0.052	2.215LysGln: 2.215 ± 0.043	2.826LysArg: 2.826 ± 0.047	3.031LysSer: 3.031 ± 0.047	2.538LysThr: 2.538 ± 0.046	3.274LysVal: 3.274 ± 0.055	0.612LysTrp: 0.612 ± 0.022	1.449LysTyr: 1.449 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	9.227LeuAla: 9.227 ± 0.107	0.76LeuCys: 0.76 ± 0.021	5.268LeuAsp: 5.268 ± 0.066	6.75LeuGlu: 6.75 ± 0.083	4.347LeuPhe: 4.347 ± 0.064	7.192LeuGly: 7.192 ± 0.076	2.021LeuHis: 2.021 ± 0.037	6.287LeuIle: 6.287 ± 0.079	5.024LeuLys: 5.024 ± 0.063	12.09LeuLeu: 12.09 ± 0.144	2.642LeuMet: 2.642 ± 0.043	3.663LeuAsn: 3.663 ± 0.052	5.035LeuPro: 5.035 ± 0.071	4.38LeuGln: 4.38 ± 0.056	5.635LeuArg: 5.635 ± 0.08	7.266LeuSer: 7.266 ± 0.072	5.742LeuThr: 5.742 ± 0.072	6.448LeuVal: 6.448 ± 0.073	1.05LeuTrp: 1.05 ± 0.027	3.07LeuTyr: 3.07 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.227MetAla: 2.227 ± 0.039	0.121MetCys: 0.121 ± 0.009	1.502MetAsp: 1.502 ± 0.038	1.929MetGlu: 1.929 ± 0.036	0.969MetPhe: 0.969 ± 0.025	1.747MetGly: 1.747 ± 0.033	0.405MetHis: 0.405 ± 0.018	1.653MetIle: 1.653 ± 0.036	1.797MetLys: 1.797 ± 0.036	3.031MetLeu: 3.031 ± 0.046	0.805MetMet: 0.805 ± 0.024	1.382MetAsn: 1.382 ± 0.031	1.218MetPro: 1.218 ± 0.029	0.952MetGln: 0.952 ± 0.025	1.458MetArg: 1.458 ± 0.027	1.851MetSer: 1.851 ± 0.039	1.608MetThr: 1.608 ± 0.038	1.56MetVal: 1.56 ± 0.036	0.208MetTrp: 0.208 ± 0.012	0.68MetTyr: 0.68 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.873AsnAla: 2.873 ± 0.047	0.266AsnCys: 0.266 ± 0.016	1.715AsnAsp: 1.715 ± 0.033	2.322AsnGlu: 2.322 ± 0.04	1.341AsnPhe: 1.341 ± 0.037	3.417AsnGly: 3.417 ± 0.077	0.673AsnHis: 0.673 ± 0.022	2.231AsnIle: 2.231 ± 0.048	1.91AsnLys: 1.91 ± 0.044	3.215AsnLeu: 3.215 ± 0.052	0.962AsnMet: 0.962 ± 0.027	1.611AsnAsn: 1.611 ± 0.043	2.073AsnPro: 2.073 ± 0.044	1.314AsnGln: 1.314 ± 0.033	2.135AsnArg: 2.135 ± 0.04	2.351AsnSer: 2.351 ± 0.046	1.952AsnThr: 1.952 ± 0.047	2.459AsnVal: 2.459 ± 0.053	0.546AsnTrp: 0.546 ± 0.018	1.282AsnTyr: 1.282 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	4.086ProAla: 4.086 ± 0.057	0.262ProCys: 0.262 ± 0.014	2.584ProAsp: 2.584 ± 0.041	3.479ProGlu: 3.479 ± 0.056	1.86ProPhe: 1.86 ± 0.035	3.546ProGly: 3.546 ± 0.063	0.853ProHis: 0.853 ± 0.026	2.463ProIle: 2.463 ± 0.046	1.713ProLys: 1.713 ± 0.033	4.293ProLeu: 4.293 ± 0.051	1.018ProMet: 1.018 ± 0.03	1.481ProAsn: 1.481 ± 0.033	1.579ProPro: 1.579 ± 0.038	1.49ProGln: 1.49 ± 0.038	1.662ProArg: 1.662 ± 0.038	3.029ProSer: 3.029 ± 0.053	2.368ProThr: 2.368 ± 0.08	3.84ProVal: 3.84 ± 0.405	0.527ProTrp: 0.527 ± 0.019	1.419ProTyr: 1.419 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.522GlnAla: 3.522 ± 0.049	0.178GlnCys: 0.178 ± 0.01	1.592GlnAsp: 1.592 ± 0.038	2.667GlnGlu: 2.667 ± 0.051	1.421GlnPhe: 1.421 ± 0.03	2.638GlnGly: 2.638 ± 0.047	0.713GlnHis: 0.713 ± 0.022	2.02GlnIle: 2.02 ± 0.035	1.703GlnLys: 1.703 ± 0.036	4.604GlnLeu: 4.604 ± 0.064	0.967GlnMet: 0.967 ± 0.026	1.277GlnAsn: 1.277 ± 0.032	1.78GlnPro: 1.78 ± 0.033	1.978GlnGln: 1.978 ± 0.052	2.126GlnArg: 2.126 ± 0.044	2.412GlnSer: 2.412 ± 0.038	1.894GlnThr: 1.894 ± 0.035	2.366GlnVal: 2.366 ± 0.039	0.495GlnTrp: 0.495 ± 0.015	1.165GlnTyr: 1.165 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	3.84ArgAla: 3.84 ± 0.056	0.383ArgCys: 0.383 ± 0.017	2.664ArgAsp: 2.664 ± 0.046	4.208ArgGlu: 4.208 ± 0.068	2.346ArgPhe: 2.346 ± 0.044	3.521ArgGly: 3.521 ± 0.063	1.075ArgHis: 1.075 ± 0.023	3.779ArgIle: 3.779 ± 0.054	2.912ArgLys: 2.912 ± 0.048	6.184ArgLeu: 6.184 ± 0.091	1.82ArgMet: 1.82 ± 0.037	1.968ArgAsn: 1.968 ± 0.038	1.963ArgPro: 1.963 ± 0.043	2.26ArgGln: 2.26 ± 0.039	3.466ArgArg: 3.466 ± 0.061	3.874ArgSer: 3.874 ± 0.063	2.774ArgThr: 2.774 ± 0.043	3.337ArgVal: 3.337 ± 0.054	0.713ArgTrp: 0.713 ± 0.019	1.969ArgTyr: 1.969 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	5.707SerAla: 5.707 ± 0.077	0.444SerCys: 0.444 ± 0.019	3.117SerAsp: 3.117 ± 0.047	3.961SerGlu: 3.961 ± 0.059	3.08SerPhe: 3.08 ± 0.049	6.589SerGly: 6.589 ± 0.08	1.122SerHis: 1.122 ± 0.034	4.286SerIle: 4.286 ± 0.061	3.144SerLys: 3.144 ± 0.053	6.776SerLeu: 6.776 ± 0.079	1.859SerMet: 1.859 ± 0.034	2.533SerAsn: 2.533 ± 0.045	2.889SerPro: 2.889 ± 0.059	2.194SerGln: 2.194 ± 0.039	3.734SerArg: 3.734 ± 0.056	5.303SerSer: 5.303 ± 0.078	3.545SerThr: 3.545 ± 0.061	4.77SerVal: 4.77 ± 0.061	0.904SerTrp: 0.904 ± 0.025	2.321SerTyr: 2.321 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	5.242ThrAla: 5.242 ± 0.083	0.288ThrCys: 0.288 ± 0.014	2.79ThrAsp: 2.79 ± 0.043	3.141ThrGlu: 3.141 ± 0.044	2.153ThrPhe: 2.153 ± 0.041	5.105ThrGly: 5.105 ± 0.162	0.843ThrHis: 0.843 ± 0.025	3.506ThrIle: 3.506 ± 0.053	2.239ThrLys: 2.239 ± 0.041	5.315ThrLeu: 5.315 ± 0.071	1.338ThrMet: 1.338 ± 0.03	1.941ThrAsn: 1.941 ± 0.045	2.756ThrPro: 2.756 ± 0.081	1.462ThrGln: 1.462 ± 0.032	2.35ThrArg: 2.35 ± 0.039	3.543ThrSer: 3.543 ± 0.05	2.818ThrThr: 2.818 ± 0.053	4.382ThrVal: 4.382 ± 0.063	0.589ThrTrp: 0.589 ± 0.021	1.751ThrTyr: 1.751 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	5.563ValAla: 5.563 ± 0.109	0.557ValCys: 0.557 ± 0.019	3.475ValAsp: 3.475 ± 0.053	4.421ValGlu: 4.421 ± 0.092	2.766ValPhe: 2.766 ± 0.044	4.696ValGly: 4.696 ± 0.059	1.289ValHis: 1.289 ± 0.028	4.449ValIle: 4.449 ± 0.056	3.517ValLys: 3.517 ± 0.055	7.332ValLeu: 7.332 ± 0.075	1.821ValMet: 1.821 ± 0.038	2.639ValAsn: 2.639 ± 0.046	3.146ValPro: 3.146 ± 0.053	2.588ValGln: 2.588 ± 0.044	3.604ValArg: 3.604 ± 0.051	4.751ValSer: 4.751 ± 0.06	4.322ValThr: 4.322 ± 0.087	4.808ValVal: 4.808 ± 0.064	0.81ValTrp: 0.81 ± 0.023	2.309ValTyr: 2.309 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.892TrpAla: 0.892 ± 0.026	0.093TrpCys: 0.093 ± 0.007	0.617TrpAsp: 0.617 ± 0.022	0.735TrpGlu: 0.735 ± 0.026	0.523TrpPhe: 0.523 ± 0.017	0.836TrpGly: 0.836 ± 0.024	0.237TrpHis: 0.237 ± 0.013	0.878TrpIle: 0.878 ± 0.025	0.729TrpLys: 0.729 ± 0.021	1.533TrpLeu: 1.533 ± 0.035	0.445TrpMet: 0.445 ± 0.018	0.641TrpAsn: 0.641 ± 0.023	0.385TrpPro: 0.385 ± 0.019	0.502TrpGln: 0.502 ± 0.021	0.674TrpArg: 0.674 ± 0.019	0.902TrpSer: 0.902 ± 0.024	0.75TrpThr: 0.75 ± 0.023	0.709TrpVal: 0.709 ± 0.023	0.174TrpTrp: 0.174 ± 0.012	0.387TrpTyr: 0.387 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.476TyrAla: 2.476 ± 0.044	0.256TyrCys: 0.256 ± 0.012	1.758TyrAsp: 1.758 ± 0.036	2.17TyrGlu: 2.17 ± 0.04	1.418TyrPhe: 1.418 ± 0.031	2.651TyrGly: 2.651 ± 0.045	0.567TyrHis: 0.567 ± 0.02	1.878TyrIle: 1.878 ± 0.036	1.417TyrLys: 1.417 ± 0.031	3.215TyrLeu: 3.215 ± 0.052	0.814TyrMet: 0.814 ± 0.022	1.307TyrAsn: 1.307 ± 0.032	1.43TyrPro: 1.43 ± 0.033	1.028TyrGln: 1.028 ± 0.026	2.076TyrArg: 2.076 ± 0.041	2.216TyrSer: 2.216 ± 0.04	1.775TyrThr: 1.775 ± 0.04	2.235TyrVal: 2.235 ± 0.04	0.423TyrTrp: 0.423 ± 0.016	1.227TyrTyr: 1.227 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4781 proteins (1546274 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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