Amino acid dipepetide frequency for Mycobacterium sp. (strain KMS)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.433AlaAla: 20.433 ± 0.174	0.977AlaCys: 0.977 ± 0.025	8.81AlaAsp: 8.81 ± 0.085	8.036AlaGlu: 8.036 ± 0.09	3.547AlaPhe: 3.547 ± 0.05	11.8AlaGly: 11.8 ± 0.095	2.662AlaHis: 2.662 ± 0.043	4.938AlaIle: 4.938 ± 0.063	2.855AlaLys: 2.855 ± 0.043	13.103AlaLeu: 13.103 ± 0.111	2.911AlaMet: 2.911 ± 0.041	2.477AlaAsn: 2.477 ± 0.039	6.64AlaPro: 6.64 ± 0.087	3.906AlaGln: 3.906 ± 0.051	8.804AlaArg: 8.804 ± 0.081	5.75AlaSer: 5.75 ± 0.061	7.187AlaThr: 7.187 ± 0.056	12.337AlaVal: 12.337 ± 0.122	1.664AlaTrp: 1.664 ± 0.029	2.324AlaTyr: 2.324 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	1.118CysAla: 1.118 ± 0.023	0.093CysCys: 0.093 ± 0.008	0.549CysAsp: 0.549 ± 0.017	0.415CysGlu: 0.415 ± 0.016	0.215CysPhe: 0.215 ± 0.009	0.925CysGly: 0.925 ± 0.025	0.191CysHis: 0.191 ± 0.01	0.248CysIle: 0.248 ± 0.012	0.116CysLys: 0.116 ± 0.008	0.65CysLeu: 0.65 ± 0.018	0.128CysMet: 0.128 ± 0.008	0.162CysAsn: 0.162 ± 0.01	0.526CysPro: 0.526 ± 0.019	0.223CysGln: 0.223 ± 0.011	0.66CysArg: 0.66 ± 0.02	0.48CysSer: 0.48 ± 0.017	0.46CysThr: 0.46 ± 0.016	0.685CysVal: 0.685 ± 0.02	0.129CysTrp: 0.129 ± 0.009	0.176CysTyr: 0.176 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.961AspAla: 7.961 ± 0.065	0.463AspCys: 0.463 ± 0.015	4.623AspAsp: 4.623 ± 0.063	4.341AspGlu: 4.341 ± 0.054	1.79AspPhe: 1.79 ± 0.032	6.22AspGly: 6.22 ± 0.064	1.525AspHis: 1.525 ± 0.028	2.577AspIle: 2.577 ± 0.036	1.234AspLys: 1.234 ± 0.025	6.191AspLeu: 6.191 ± 0.056	1.035AspMet: 1.035 ± 0.026	1.226AspAsn: 1.226 ± 0.024	4.65AspPro: 4.65 ± 0.046	1.699AspGln: 1.699 ± 0.029	4.951AspArg: 4.951 ± 0.055	2.673AspSer: 2.673 ± 0.039	3.432AspThr: 3.432 ± 0.044	5.56AspVal: 5.56 ± 0.058	1.001AspTrp: 1.001 ± 0.023	1.39AspTyr: 1.39 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	6.407GluAla: 6.407 ± 0.072	0.385GluCys: 0.385 ± 0.012	2.692GluAsp: 2.692 ± 0.047	2.671GluGlu: 2.671 ± 0.046	1.857GluPhe: 1.857 ± 0.031	3.409GluGly: 3.409 ± 0.047	1.5GluHis: 1.5 ± 0.035	2.588GluIle: 2.588 ± 0.037	1.37GluLys: 1.37 ± 0.03	6.368GluLeu: 6.368 ± 0.072	1.07GluMet: 1.07 ± 0.022	1.107GluAsn: 1.107 ± 0.029	3.233GluPro: 3.233 ± 0.048	2.236GluGln: 2.236 ± 0.038	4.618GluArg: 4.618 ± 0.057	2.779GluSer: 2.779 ± 0.042	2.745GluThr: 2.745 ± 0.044	4.615GluVal: 4.615 ± 0.051	0.811GluTrp: 0.811 ± 0.021	1.124GluTyr: 1.124 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.98PheAla: 3.98 ± 0.053	0.307PheCys: 0.307 ± 0.014	2.54PheAsp: 2.54 ± 0.039	1.563PheGlu: 1.563 ± 0.031	0.978PhePhe: 0.978 ± 0.025	3.387PheGly: 3.387 ± 0.041	0.634PheHis: 0.634 ± 0.019	1.072PheIle: 1.072 ± 0.025	0.506PheLys: 0.506 ± 0.018	2.535PheLeu: 2.535 ± 0.04	0.504PheMet: 0.504 ± 0.016	0.707PheAsn: 0.707 ± 0.019	1.429PhePro: 1.429 ± 0.03	0.655PheGln: 0.655 ± 0.019	1.714PheArg: 1.714 ± 0.034	1.616PheSer: 1.616 ± 0.03	2.116PheThr: 2.116 ± 0.03	2.59PheVal: 2.59 ± 0.039	0.447PheTrp: 0.447 ± 0.016	0.725PheTyr: 0.725 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	10.429GlyAla: 10.429 ± 0.1	0.813GlyCys: 0.813 ± 0.02	5.277GlyAsp: 5.277 ± 0.063	4.522GlyGlu: 4.522 ± 0.049	3.022GlyPhe: 3.022 ± 0.041	8.015GlyGly: 8.015 ± 0.122	2.053GlyHis: 2.053 ± 0.037	3.873GlyIle: 3.873 ± 0.053	2.103GlyLys: 2.103 ± 0.04	8.545GlyLeu: 8.545 ± 0.073	2.173GlyMet: 2.173 ± 0.033	1.83GlyAsn: 1.83 ± 0.038	4.542GlyPro: 4.542 ± 0.053	2.765GlyGln: 2.765 ± 0.041	6.667GlyArg: 6.667 ± 0.072	4.982GlySer: 4.982 ± 0.055	5.052GlyThr: 5.052 ± 0.06	7.839GlyVal: 7.839 ± 0.06	1.673GlyTrp: 1.673 ± 0.03	2.325GlyTyr: 2.325 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	2.46HisAla: 2.46 ± 0.038	0.223HisCys: 0.223 ± 0.012	1.406HisAsp: 1.406 ± 0.029	1.054HisGlu: 1.054 ± 0.025	0.701HisPhe: 0.701 ± 0.019	2.098HisGly: 2.098 ± 0.036	0.749HisHis: 0.749 ± 0.024	0.8HisIle: 0.8 ± 0.022	0.35HisLys: 0.35 ± 0.013	2.214HisLeu: 2.214 ± 0.032	0.368HisMet: 0.368 ± 0.016	0.457HisAsn: 0.457 ± 0.016	1.696HisPro: 1.696 ± 0.033	0.608HisGln: 0.608 ± 0.018	2.049HisArg: 2.049 ± 0.036	1.1HisSer: 1.1 ± 0.025	1.277HisThr: 1.277 ± 0.027	1.706HisVal: 1.706 ± 0.03	0.395HisTrp: 0.395 ± 0.013	0.556HisTyr: 0.556 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	6.197IleAla: 6.197 ± 0.065	0.383IleCys: 0.383 ± 0.015	3.29IleAsp: 3.29 ± 0.041	2.527IleGlu: 2.527 ± 0.042	0.984IlePhe: 0.984 ± 0.024	4.348IleGly: 4.348 ± 0.053	0.771IleHis: 0.771 ± 0.02	1.329IleIle: 1.329 ± 0.028	0.808IleLys: 0.808 ± 0.021	2.92IleLeu: 2.92 ± 0.042	0.586IleMet: 0.586 ± 0.018	1.019IleAsn: 1.019 ± 0.025	2.261IlePro: 2.261 ± 0.038	0.868IleGln: 0.868 ± 0.023	2.678IleArg: 2.678 ± 0.037	2.073IleSer: 2.073 ± 0.033	2.635IleThr: 2.635 ± 0.039	3.641IleVal: 3.641 ± 0.045	0.417IleTrp: 0.417 ± 0.015	0.747IleTyr: 0.747 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	2.572LysAla: 2.572 ± 0.039	0.117LysCys: 0.117 ± 0.009	1.083LysAsp: 1.083 ± 0.028	0.945LysGlu: 0.945 ± 0.027	0.551LysPhe: 0.551 ± 0.017	1.508LysGly: 1.508 ± 0.03	0.45LysHis: 0.45 ± 0.015	0.932LysIle: 0.932 ± 0.025	0.648LysLys: 0.648 ± 0.027	2.06LysLeu: 2.06 ± 0.033	0.486LysMet: 0.486 ± 0.017	0.514LysAsn: 0.514 ± 0.015	1.343LysPro: 1.343 ± 0.032	0.681LysGln: 0.681 ± 0.017	1.617LysArg: 1.617 ± 0.026	1.197LysSer: 1.197 ± 0.03	1.331LysThr: 1.331 ± 0.028	1.845LysVal: 1.845 ± 0.041	0.304LysTrp: 0.304 ± 0.01	0.45LysTyr: 0.45 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	13.781LeuAla: 13.781 ± 0.109	0.79LeuCys: 0.79 ± 0.022	6.594LeuAsp: 6.594 ± 0.06	4.486LeuGlu: 4.486 ± 0.055	2.68LeuPhe: 2.68 ± 0.04	8.559LeuGly: 8.559 ± 0.076	2.048LeuHis: 2.048 ± 0.035	4.03LeuIle: 4.03 ± 0.051	1.722LeuLys: 1.722 ± 0.037	9.567LeuLeu: 9.567 ± 0.092	1.773LeuMet: 1.773 ± 0.036	2.005LeuAsn: 2.005 ± 0.039	5.714LeuPro: 5.714 ± 0.062	2.506LeuGln: 2.506 ± 0.034	7.72LeuArg: 7.72 ± 0.076	5.516LeuSer: 5.516 ± 0.054	6.525LeuThr: 6.525 ± 0.062	8.276LeuVal: 8.276 ± 0.074	1.235LeuTrp: 1.235 ± 0.027	1.679LeuTyr: 1.679 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	2.674MetAla: 2.674 ± 0.036	0.192MetCys: 0.192 ± 0.01	0.886MetAsp: 0.886 ± 0.022	0.712MetGlu: 0.712 ± 0.02	0.646MetPhe: 0.646 ± 0.019	1.502MetGly: 1.502 ± 0.032	0.409MetHis: 0.409 ± 0.016	0.843MetIle: 0.843 ± 0.022	0.455MetLys: 0.455 ± 0.016	2.027MetLeu: 2.027 ± 0.031	0.441MetMet: 0.441 ± 0.017	0.486MetAsn: 0.486 ± 0.017	1.258MetPro: 1.258 ± 0.028	0.54MetGln: 0.54 ± 0.017	1.636MetArg: 1.636 ± 0.03	1.594MetSer: 1.594 ± 0.026	1.911MetThr: 1.911 ± 0.032	1.652MetVal: 1.652 ± 0.029	0.276MetTrp: 0.276 ± 0.011	0.335MetTyr: 0.335 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.578AsnAla: 2.578 ± 0.042	0.177AsnCys: 0.177 ± 0.012	1.188AsnAsp: 1.188 ± 0.026	0.964AsnGlu: 0.964 ± 0.023	0.649AsnPhe: 0.649 ± 0.021	1.985AsnGly: 1.985 ± 0.044	0.452AsnHis: 0.452 ± 0.016	0.879AsnIle: 0.879 ± 0.023	0.438AsnLys: 0.438 ± 0.018	1.977AsnLeu: 1.977 ± 0.036	0.41AsnMet: 0.41 ± 0.015	0.523AsnAsn: 0.523 ± 0.018	1.7AsnPro: 1.7 ± 0.032	0.643AsnGln: 0.643 ± 0.02	1.604AsnArg: 1.604 ± 0.028	1.066AsnSer: 1.066 ± 0.021	1.264AsnThr: 1.264 ± 0.026	1.728AsnVal: 1.728 ± 0.03	0.38AsnTrp: 0.38 ± 0.012	0.527AsnTyr: 0.527 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	7.564ProAla: 7.564 ± 0.085	0.312ProCys: 0.312 ± 0.014	4.532ProAsp: 4.532 ± 0.051	3.762ProGlu: 3.762 ± 0.049	1.678ProPhe: 1.678 ± 0.03	5.701ProGly: 5.701 ± 0.068	1.312ProHis: 1.312 ± 0.029	2.053ProIle: 2.053 ± 0.038	1.278ProLys: 1.278 ± 0.033	4.91ProLeu: 4.91 ± 0.056	1.218ProMet: 1.218 ± 0.027	1.236ProAsn: 1.236 ± 0.027	4.155ProPro: 4.155 ± 0.102	1.893ProGln: 1.893 ± 0.033	3.628ProArg: 3.628 ± 0.043	3.097ProSer: 3.097 ± 0.045	3.532ProThr: 3.532 ± 0.052	5.339ProVal: 5.339 ± 0.061	0.87ProTrp: 0.87 ± 0.021	1.209ProTyr: 1.209 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.509GlnAla: 3.509 ± 0.046	0.23GlnCys: 0.23 ± 0.011	1.171GlnAsp: 1.171 ± 0.022	1.14GlnGlu: 1.14 ± 0.025	1.001GlnPhe: 1.001 ± 0.022	1.974GlnGly: 1.974 ± 0.038	0.69GlnHis: 0.69 ± 0.016	1.393GlnIle: 1.393 ± 0.028	0.593GlnLys: 0.593 ± 0.018	3.171GlnLeu: 3.171 ± 0.041	0.728GlnMet: 0.728 ± 0.022	0.608GlnAsn: 0.608 ± 0.017	1.892GlnPro: 1.892 ± 0.037	1.301GlnGln: 1.301 ± 0.038	2.831GlnArg: 2.831 ± 0.041	1.439GlnSer: 1.439 ± 0.029	1.632GlnThr: 1.632 ± 0.032	2.395GlnVal: 2.395 ± 0.036	0.618GlnTrp: 0.618 ± 0.019	0.671GlnTyr: 0.671 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	8.555ArgAla: 8.555 ± 0.081	0.662ArgCys: 0.662 ± 0.018	4.595ArgAsp: 4.595 ± 0.046	4.039ArgGlu: 4.039 ± 0.051	2.453ArgPhe: 2.453 ± 0.036	5.412ArgGly: 5.412 ± 0.062	1.936ArgHis: 1.936 ± 0.037	3.539ArgIle: 3.539 ± 0.043	1.564ArgLys: 1.564 ± 0.034	7.711ArgLeu: 7.711 ± 0.078	1.829ArgMet: 1.829 ± 0.026	1.662ArgAsn: 1.662 ± 0.032	4.243ArgPro: 4.243 ± 0.059	2.315ArgGln: 2.315 ± 0.04	7.318ArgArg: 7.318 ± 0.077	4.079ArgSer: 4.079 ± 0.05	4.524ArgThr: 4.524 ± 0.05	5.748ArgVal: 5.748 ± 0.056	1.457ArgTrp: 1.457 ± 0.028	1.956ArgTyr: 1.956 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.989SerAla: 6.989 ± 0.065	0.433SerCys: 0.433 ± 0.017	3.185SerAsp: 3.185 ± 0.045	2.588SerGlu: 2.588 ± 0.042	1.531SerPhe: 1.531 ± 0.026	5.503SerGly: 5.503 ± 0.056	1.047SerHis: 1.047 ± 0.026	2.007SerIle: 2.007 ± 0.031	1.048SerLys: 1.048 ± 0.025	4.597SerLeu: 4.597 ± 0.057	1.312SerMet: 1.312 ± 0.023	1.048SerAsn: 1.048 ± 0.026	3.091SerPro: 3.091 ± 0.041	1.378SerGln: 1.378 ± 0.027	3.779SerArg: 3.779 ± 0.048	2.99SerSer: 2.99 ± 0.05	3.33SerThr: 3.33 ± 0.04	4.529SerVal: 4.529 ± 0.051	0.899SerTrp: 0.899 ± 0.023	1.17SerTyr: 1.17 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	8.161ThrAla: 8.161 ± 0.073	0.424ThrCys: 0.424 ± 0.016	3.869ThrAsp: 3.869 ± 0.039	3.219ThrGlu: 3.219 ± 0.039	1.806ThrPhe: 1.806 ± 0.031	5.907ThrGly: 5.907 ± 0.058	1.157ThrHis: 1.157 ± 0.027	2.292ThrIle: 2.292 ± 0.035	1.166ThrLys: 1.166 ± 0.027	5.613ThrLeu: 5.613 ± 0.055	1.224ThrMet: 1.224 ± 0.025	1.181ThrAsn: 1.181 ± 0.028	4.04ThrPro: 4.04 ± 0.051	1.467ThrGln: 1.467 ± 0.029	3.869ThrArg: 3.869 ± 0.046	3.19ThrSer: 3.19 ± 0.043	3.912ThrThr: 3.912 ± 0.066	6.228ThrVal: 6.228 ± 0.057	0.865ThrTrp: 0.865 ± 0.019	1.303ThrTyr: 1.303 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	11.77ValAla: 11.77 ± 0.108	0.764ValCys: 0.764 ± 0.022	6.045ValAsp: 6.045 ± 0.061	4.825ValGlu: 4.825 ± 0.051	2.611ValPhe: 2.611 ± 0.041	7.175ValGly: 7.175 ± 0.068	1.85ValHis: 1.85 ± 0.033	3.761ValIle: 3.761 ± 0.044	1.675ValLys: 1.675 ± 0.035	9.006ValLeu: 9.006 ± 0.072	1.624ValMet: 1.624 ± 0.028	2.038ValAsn: 2.038 ± 0.037	4.762ValPro: 4.762 ± 0.055	2.035ValGln: 2.035 ± 0.031	6.195ValArg: 6.195 ± 0.065	4.783ValSer: 4.783 ± 0.055	5.865ValThr: 5.865 ± 0.057	8.734ValVal: 8.734 ± 0.081	1.127ValTrp: 1.127 ± 0.026	1.626ValTyr: 1.626 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.598TrpAla: 1.598 ± 0.031	0.165TrpCys: 0.165 ± 0.01	0.811TrpAsp: 0.811 ± 0.018	0.688TrpGlu: 0.688 ± 0.02	0.545TrpPhe: 0.545 ± 0.017	1.034TrpGly: 1.034 ± 0.024	0.356TrpHis: 0.356 ± 0.013	0.669TrpIle: 0.669 ± 0.02	0.338TrpLys: 0.338 ± 0.014	1.727TrpLeu: 1.727 ± 0.034	0.357TrpMet: 0.357 ± 0.014	0.398TrpAsn: 0.398 ± 0.016	0.845TrpPro: 0.845 ± 0.024	0.635TrpGln: 0.635 ± 0.019	1.369TrpArg: 1.369 ± 0.024	0.945TrpSer: 0.945 ± 0.025	0.962TrpThr: 0.962 ± 0.019	1.152TrpVal: 1.152 ± 0.028	0.359TrpTrp: 0.359 ± 0.015	0.324TrpTyr: 0.324 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.427TyrAla: 2.427 ± 0.035	0.237TyrCys: 0.237 ± 0.011	1.398TyrAsp: 1.398 ± 0.031	1.087TyrGlu: 1.087 ± 0.026	0.761TyrPhe: 0.761 ± 0.021	2.036TyrGly: 2.036 ± 0.037	0.477TyrHis: 0.477 ± 0.016	0.66TyrIle: 0.66 ± 0.022	0.367TyrLys: 0.367 ± 0.017	2.313TyrLeu: 2.313 ± 0.035	0.3TyrMet: 0.3 ± 0.012	0.447TyrAsn: 0.447 ± 0.016	1.257TyrPro: 1.257 ± 0.027	0.632TyrGln: 0.632 ± 0.019	1.891TyrArg: 1.891 ± 0.032	1.104TyrSer: 1.104 ± 0.026	1.2TyrThr: 1.2 ± 0.029	1.671TyrVal: 1.671 ± 0.035	0.354TyrTrp: 0.354 ± 0.014	0.484TyrTyr: 0.484 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5893 proteins (1899582 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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