Burkholderiales bacterium YL45

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2449 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C7HIK0|A0A1C7HIK0_9BURK Uncharacterized protein OS=Burkholderiales bacterium YL45 OX=1834205 GN=A4V04_06055 PE=4 SV=1
MM1 pKa = 6.98EE2 pKa = 5.08QIEE5 pKa = 4.53ISPLPWQEE13 pKa = 3.58FSVVLDD19 pKa = 3.58SQNCVIQLRR28 pKa = 11.84QVAEE32 pKa = 4.07SLYY35 pKa = 11.12CDD37 pKa = 4.09LTCNEE42 pKa = 3.81IEE44 pKa = 3.98IFKK47 pKa = 10.33GRR49 pKa = 11.84KK50 pKa = 7.94VCVGTDD56 pKa = 3.34INCYY60 pKa = 9.61PSPNFNGRR68 pKa = 11.84LYY70 pKa = 11.0FIDD73 pKa = 3.84TLGQSDD79 pKa = 4.01PQYY82 pKa = 11.24EE83 pKa = 4.3EE84 pKa = 5.84LNTRR88 pKa = 11.84WLLLYY93 pKa = 10.4EE94 pKa = 4.48STDD97 pKa = 3.52SEE99 pKa = 4.67ASNEE103 pKa = 4.01QQ104 pKa = 3.17

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C7HH83|A0A1C7HH83_9BURK DDE_Tnp_1 domain-containing protein OS=Burkholderiales bacterium YL45 OX=1834205 GN=A4V04_09850 PE=4 SV=1
MM1 pKa = 6.85ATKK4 pKa = 9.51RR5 pKa = 11.84TYY7 pKa = 10.39QPSKK11 pKa = 8.92VKK13 pKa = 10.35RR14 pKa = 11.84ARR16 pKa = 11.84THH18 pKa = 5.71GFLVRR23 pKa = 11.84SRR25 pKa = 11.84TRR27 pKa = 11.84HH28 pKa = 4.09GRR30 pKa = 11.84KK31 pKa = 9.02VLAARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.13GRR41 pKa = 11.84HH42 pKa = 3.97VLALL46 pKa = 3.77

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2449

0

2449

802378

27

1855

327.6

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.448 ± 0.059

1.339 ± 0.022

4.919 ± 0.039

6.636 ± 0.054

4.494 ± 0.039

7.17 ± 0.051

1.738 ± 0.019

6.378 ± 0.034

6.979 ± 0.051

10.011 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.024

4.204 ± 0.034

4.337 ± 0.029

3.479 ± 0.03

4.639 ± 0.044

6.616 ± 0.048

5.158 ± 0.03

6.99 ± 0.045

1.193 ± 0.019

2.858 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski