Rhodobacter sp. 24-YEA-8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter; unclassified Rhodobacter

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4521 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H4TB81|A0A1H4TB81_9RHOB ElaA protein OS=Rhodobacter sp. 24-YEA-8 OX=1884310 GN=SAMN05519105_2766 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.18KK3 pKa = 10.52LLIATTALVAGASAAAADD21 pKa = 4.1VTISGYY27 pKa = 11.03GRR29 pKa = 11.84FGLQYY34 pKa = 10.16IEE36 pKa = 4.89DD37 pKa = 3.83RR38 pKa = 11.84GVTPGSGIAGTEE50 pKa = 4.06EE51 pKa = 3.86EE52 pKa = 4.33TQIAGRR58 pKa = 11.84LRR60 pKa = 11.84LNIDD64 pKa = 3.16ATTEE68 pKa = 3.83TDD70 pKa = 3.26VGVTFGARR78 pKa = 11.84LRR80 pKa = 11.84LQNDD84 pKa = 4.28LGNDD88 pKa = 3.39TTVGNKK94 pKa = 9.28ARR96 pKa = 11.84FSVGYY101 pKa = 9.97EE102 pKa = 3.78GLTVQVGNVDD112 pKa = 3.92TAWDD116 pKa = 4.15SVRR119 pKa = 11.84LTYY122 pKa = 10.55DD123 pKa = 2.91SEE125 pKa = 4.34MGFDD129 pKa = 4.12DD130 pKa = 5.46SSFGDD135 pKa = 3.52AQNGFFAYY143 pKa = 9.86NSKK146 pKa = 10.83GADD149 pKa = 3.22SNQNSNYY156 pKa = 10.22AGVAAFYY163 pKa = 10.84SIADD167 pKa = 3.44VNLYY171 pKa = 10.16FSYY174 pKa = 11.23VDD176 pKa = 3.64PDD178 pKa = 3.42QTAKK182 pKa = 10.75DD183 pKa = 3.77LSIVPGLLANDD194 pKa = 3.92EE195 pKa = 4.73EE196 pKa = 5.12IGLAADD202 pKa = 4.02WSNGQISVAAGINLSSQGIKK222 pKa = 10.98DD223 pKa = 3.43NDD225 pKa = 3.92DD226 pKa = 3.72YY227 pKa = 11.7FIGAAYY233 pKa = 10.66NFGDD237 pKa = 3.85GNVGLNYY244 pKa = 10.11YY245 pKa = 10.25DD246 pKa = 4.31YY247 pKa = 9.38NTAAGGVSDD256 pKa = 3.9STQVTLYY263 pKa = 11.25GNYY266 pKa = 9.26TFGATTLKK274 pKa = 10.68AYY276 pKa = 10.63VSDD279 pKa = 3.83WDD281 pKa = 3.68RR282 pKa = 11.84AGYY285 pKa = 7.86DD286 pKa = 3.12TAYY289 pKa = 10.66GIGADD294 pKa = 3.68YY295 pKa = 11.25SLGEE299 pKa = 4.17GARR302 pKa = 11.84ISGSIQSGFGDD313 pKa = 3.4AAGANNGTKK322 pKa = 10.19ADD324 pKa = 3.75LGVRR328 pKa = 11.84FDD330 pKa = 3.67FF331 pKa = 5.43

Molecular weight: 34.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5AHU7|A0A1H5AHU7_9RHOB Alkyl hydroperoxide reductase C OS=Rhodobacter sp. 24-YEA-8 OX=1884310 GN=SAMN05519105_3942 PE=3 SV=1
MM1 pKa = 7.57PRR3 pKa = 11.84RR4 pKa = 11.84PRR6 pKa = 11.84VLRR9 pKa = 11.84LRR11 pKa = 11.84IGRR14 pKa = 11.84IVNLSQIVVIAILTIIIGNHH34 pKa = 4.3GG35 pKa = 3.44

Molecular weight: 3.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4521

0

4521

1432208

29

3632

316.8

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.865 ± 0.053

0.853 ± 0.011

5.563 ± 0.03

5.694 ± 0.027

3.639 ± 0.022

9.056 ± 0.048

1.958 ± 0.017

5.267 ± 0.025

2.879 ± 0.027

10.507 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.017

2.419 ± 0.021

5.349 ± 0.031

3.104 ± 0.018

7.113 ± 0.038

5.321 ± 0.022

5.276 ± 0.022

6.972 ± 0.031

1.466 ± 0.016

2.056 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski