Amino acid dipepetide frequency for Rhodobacter sp. 24-YEA-8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.228AlaAla: 18.228 ± 0.208
1.066AlaCys: 1.066 ± 0.029
6.804AlaAsp: 6.804 ± 0.075
8.156AlaGlu: 8.156 ± 0.097
4.292AlaPhe: 4.292 ± 0.064
12.171AlaGly: 12.171 ± 0.117
2.171AlaHis: 2.171 ± 0.043
5.859AlaIle: 5.859 ± 0.067
3.812AlaLys: 3.812 ± 0.058
14.879AlaLeu: 14.879 ± 0.119
3.718AlaMet: 3.718 ± 0.053
2.515AlaAsn: 2.515 ± 0.043
6.377AlaPro: 6.377 ± 0.085
4.114AlaGln: 4.114 ± 0.06
9.836AlaArg: 9.836 ± 0.093
6.15AlaSer: 6.15 ± 0.067
5.964AlaThr: 5.964 ± 0.07
8.504AlaVal: 8.504 ± 0.096
1.859AlaTrp: 1.859 ± 0.039
2.175AlaTyr: 2.175 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
0.99CysAla: 0.99 ± 0.027
0.102CysCys: 0.102 ± 0.009
0.573CysAsp: 0.573 ± 0.018
0.463CysGlu: 0.463 ± 0.018
0.31CysPhe: 0.31 ± 0.017
0.928CysGly: 0.928 ± 0.03
0.253CysHis: 0.253 ± 0.013
0.406CysIle: 0.406 ± 0.015
0.184CysLys: 0.184 ± 0.013
0.931CysLeu: 0.931 ± 0.029
0.172CysMet: 0.172 ± 0.012
0.194CysAsn: 0.194 ± 0.011
0.468CysPro: 0.468 ± 0.019
0.229CysGln: 0.229 ± 0.013
0.582CysArg: 0.582 ± 0.019
0.447CysSer: 0.447 ± 0.02
0.422CysThr: 0.422 ± 0.018
0.55CysVal: 0.55 ± 0.021
0.126CysTrp: 0.126 ± 0.009
0.197CysTyr: 0.197 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.186AspAla: 6.186 ± 0.076
0.525AspCys: 0.525 ± 0.022
2.655AspAsp: 2.655 ± 0.052
3.249AspGlu: 3.249 ± 0.052
2.325AspPhe: 2.325 ± 0.041
4.977AspGly: 4.977 ± 0.109
1.359AspHis: 1.359 ± 0.031
2.787AspIle: 2.787 ± 0.052
1.304AspLys: 1.304 ± 0.031
6.852AspLeu: 6.852 ± 0.085
1.433AspMet: 1.433 ± 0.032
1.096AspAsn: 1.096 ± 0.03
3.867AspPro: 3.867 ± 0.053
2.162AspGln: 2.162 ± 0.038
4.168AspArg: 4.168 ± 0.057
2.16AspSer: 2.16 ± 0.041
2.439AspThr: 2.439 ± 0.046
3.443AspVal: 3.443 ± 0.058
1.158AspTrp: 1.158 ± 0.034
1.482AspTyr: 1.482 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
8.689GluAla: 8.689 ± 0.106
0.351GluCys: 0.351 ± 0.014
2.897GluAsp: 2.897 ± 0.046
3.379GluGlu: 3.379 ± 0.053
1.752GluPhe: 1.752 ± 0.034
4.915GluGly: 4.915 ± 0.067
0.929GluHis: 0.929 ± 0.026
3.823GluIle: 3.823 ± 0.053
2.085GluLys: 2.085 ± 0.042
4.911GluLeu: 4.911 ± 0.056
1.69GluMet: 1.69 ± 0.034
1.563GluAsn: 1.563 ± 0.035
2.5GluPro: 2.5 ± 0.047
1.643GluGln: 1.643 ± 0.035
3.942GluArg: 3.942 ± 0.063
2.245GluSer: 2.245 ± 0.042
3.825GluThr: 3.825 ± 0.049
4.19GluVal: 4.19 ± 0.052
0.679GluTrp: 0.679 ± 0.024
0.931GluTyr: 0.931 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.67PheAla: 4.67 ± 0.067
0.415PheCys: 0.415 ± 0.017
2.558PheAsp: 2.558 ± 0.047
1.876PheGlu: 1.876 ± 0.034
1.365PhePhe: 1.365 ± 0.038
3.735PheGly: 3.735 ± 0.053
0.735PheHis: 0.735 ± 0.025
1.676PheIle: 1.676 ± 0.038
0.66PheLys: 0.66 ± 0.022
3.565PheLeu: 3.565 ± 0.053
0.788PheMet: 0.788 ± 0.023
0.969PheAsn: 0.969 ± 0.029
1.676PhePro: 1.676 ± 0.04
1.008PheGln: 1.008 ± 0.027
2.512PheArg: 2.512 ± 0.04
2.232PheSer: 2.232 ± 0.04
2.206PheThr: 2.206 ± 0.041
2.311PheVal: 2.311 ± 0.043
0.587PheTrp: 0.587 ± 0.022
0.844PheTyr: 0.844 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
10.494GlyAla: 10.494 ± 0.119
0.841GlyCys: 0.841 ± 0.032
4.397GlyAsp: 4.397 ± 0.125
5.075GlyGlu: 5.075 ± 0.056
3.874GlyPhe: 3.874 ± 0.056
7.906GlyGly: 7.906 ± 0.112
1.935GlyHis: 1.935 ± 0.039
4.985GlyIle: 4.985 ± 0.057
3.109GlyLys: 3.109 ± 0.055
10.181GlyLeu: 10.181 ± 0.098
2.678GlyMet: 2.678 ± 0.046
2.185GlyAsn: 2.185 ± 0.053
4.062GlyPro: 4.062 ± 0.061
3.331GlyGln: 3.331 ± 0.053
6.486GlyArg: 6.486 ± 0.062
4.537GlySer: 4.537 ± 0.063
4.535GlyThr: 4.535 ± 0.065
6.162GlyVal: 6.162 ± 0.071
1.537GlyTrp: 1.537 ± 0.038
2.248GlyTyr: 2.248 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.122HisAla: 2.122 ± 0.045
0.225HisCys: 0.225 ± 0.015
1.19HisAsp: 1.19 ± 0.031
1.083HisGlu: 1.083 ± 0.03
0.85HisPhe: 0.85 ± 0.026
1.837HisGly: 1.837 ± 0.04
0.537HisHis: 0.537 ± 0.022
0.987HisIle: 0.987 ± 0.03
0.456HisLys: 0.456 ± 0.018
2.112HisLeu: 2.112 ± 0.044
0.505HisMet: 0.505 ± 0.02
0.388HisAsn: 0.388 ± 0.017
1.381HisPro: 1.381 ± 0.032
0.653HisGln: 0.653 ± 0.025
1.378HisArg: 1.378 ± 0.032
0.985HisSer: 0.985 ± 0.029
0.688HisThr: 0.688 ± 0.022
1.325HisVal: 1.325 ± 0.031
0.334HisTrp: 0.334 ± 0.017
0.539HisTyr: 0.539 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.412IleAla: 7.412 ± 0.075
0.65IleCys: 0.65 ± 0.02
3.221IleAsp: 3.221 ± 0.052
3.206IleGlu: 3.206 ± 0.051
1.838IlePhe: 1.838 ± 0.036
5.257IleGly: 5.257 ± 0.066
0.924IleHis: 0.924 ± 0.028
2.235IleIle: 2.235 ± 0.044
0.975IleLys: 0.975 ± 0.026
5.117IleLeu: 5.117 ± 0.061
1.033IleMet: 1.033 ± 0.027
1.33IleAsn: 1.33 ± 0.03
2.505IlePro: 2.505 ± 0.049
1.178IleGln: 1.178 ± 0.029
4.145IleArg: 4.145 ± 0.053
3.344IleSer: 3.344 ± 0.045
3.111IleThr: 3.111 ± 0.048
3.268IleVal: 3.268 ± 0.054
0.836IleTrp: 0.836 ± 0.028
1.083IleTyr: 1.083 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
3.745LysAla: 3.745 ± 0.061
0.151LysCys: 0.151 ± 0.011
1.558LysAsp: 1.558 ± 0.035
1.357LysGlu: 1.357 ± 0.037
0.816LysPhe: 0.816 ± 0.029
2.715LysGly: 2.715 ± 0.046
0.51LysHis: 0.51 ± 0.02
1.619LysIle: 1.619 ± 0.035
1.038LysLys: 1.038 ± 0.034
2.806LysLeu: 2.806 ± 0.048
0.77LysMet: 0.77 ± 0.023
0.739LysAsn: 0.739 ± 0.024
1.707LysPro: 1.707 ± 0.04
0.773LysGln: 0.773 ± 0.024
1.973LysArg: 1.973 ± 0.044
1.755LysSer: 1.755 ± 0.033
1.721LysThr: 1.721 ± 0.037
2.125LysVal: 2.125 ± 0.047
0.356LysTrp: 0.356 ± 0.018
0.551LysTyr: 0.551 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
14.75LeuAla: 14.75 ± 0.116
0.947LeuCys: 0.947 ± 0.028
5.664LeuAsp: 5.664 ± 0.068
5.142LeuGlu: 5.142 ± 0.072
3.462LeuPhe: 3.462 ± 0.057
8.481LeuGly: 8.481 ± 0.099
1.928LeuHis: 1.928 ± 0.044
5.93LeuIle: 5.93 ± 0.076
3.248LeuLys: 3.248 ± 0.052
9.473LeuLeu: 9.473 ± 0.125
2.732LeuMet: 2.732 ± 0.042
2.681LeuAsn: 2.681 ± 0.047
6.232LeuPro: 6.232 ± 0.076
2.819LeuGln: 2.819 ± 0.045
7.733LeuArg: 7.733 ± 0.094
6.935LeuSer: 6.935 ± 0.079
6.355LeuThr: 6.355 ± 0.07
7.174LeuVal: 7.174 ± 0.072
1.389LeuTrp: 1.389 ± 0.033
1.998LeuTyr: 1.998 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
3.462MetAla: 3.462 ± 0.055
0.154MetCys: 0.154 ± 0.01
1.188MetAsp: 1.188 ± 0.029
1.267MetGlu: 1.267 ± 0.032
0.718MetPhe: 0.718 ± 0.025
2.146MetGly: 2.146 ± 0.038
0.376MetHis: 0.376 ± 0.016
1.558MetIle: 1.558 ± 0.032
0.983MetLys: 0.983 ± 0.029
2.64MetLeu: 2.64 ± 0.046
0.723MetMet: 0.723 ± 0.023
0.795MetAsn: 0.795 ± 0.024
1.477MetPro: 1.477 ± 0.036
0.962MetGln: 0.962 ± 0.026
1.94MetArg: 1.94 ± 0.036
1.699MetSer: 1.699 ± 0.036
2.026MetThr: 2.026 ± 0.034
1.841MetVal: 1.841 ± 0.036
0.193MetTrp: 0.193 ± 0.012
0.297MetTyr: 0.297 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.833AsnAla: 2.833 ± 0.046
0.214AsnCys: 0.214 ± 0.012
1.264AsnAsp: 1.264 ± 0.043
1.102AsnGlu: 1.102 ± 0.028
0.915AsnPhe: 0.915 ± 0.028
2.322AsnGly: 2.322 ± 0.042
0.499AsnHis: 0.499 ± 0.019
1.32AsnIle: 1.32 ± 0.032
0.553AsnLys: 0.553 ± 0.022
2.514AsnLeu: 2.514 ± 0.04
0.647AsnMet: 0.647 ± 0.023
0.574AsnAsn: 0.574 ± 0.02
1.82AsnPro: 1.82 ± 0.035
0.707AsnGln: 0.707 ± 0.02
1.797AsnArg: 1.797 ± 0.039
1.261AsnSer: 1.261 ± 0.029
1.19AsnThr: 1.19 ± 0.031
1.622AsnVal: 1.622 ± 0.034
0.398AsnTrp: 0.398 ± 0.015
0.635AsnTyr: 0.635 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
6.933ProAla: 6.933 ± 0.098
0.351ProCys: 0.351 ± 0.015
3.962ProAsp: 3.962 ± 0.056
4.37ProGlu: 4.37 ± 0.054
1.977ProPhe: 1.977 ± 0.043
5.732ProGly: 5.732 ± 0.069
1.068ProHis: 1.068 ± 0.028
1.885ProIle: 1.885 ± 0.035
1.562ProLys: 1.562 ± 0.037
4.918ProLeu: 4.918 ± 0.059
1.274ProMet: 1.274 ± 0.03
1.158ProAsn: 1.158 ± 0.034
2.51ProPro: 2.51 ± 0.056
1.624ProGln: 1.624 ± 0.035
2.964ProArg: 2.964 ± 0.055
2.454ProSer: 2.454 ± 0.042
2.087ProThr: 2.087 ± 0.046
4.8ProVal: 4.8 ± 0.062
0.784ProTrp: 0.784 ± 0.022
1.072ProTyr: 1.072 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
4.033GlnAla: 4.033 ± 0.055
0.205GlnCys: 0.205 ± 0.012
1.675GlnAsp: 1.675 ± 0.037
1.48GlnGlu: 1.48 ± 0.037
0.982GlnPhe: 0.982 ± 0.024
2.732GlnGly: 2.732 ± 0.047
0.553GlnHis: 0.553 ± 0.017
2.058GlnIle: 2.058 ± 0.038
1.025GlnLys: 1.025 ± 0.031
2.914GlnLeu: 2.914 ± 0.05
0.954GlnMet: 0.954 ± 0.028
0.877GlnAsn: 0.877 ± 0.03
1.771GlnPro: 1.771 ± 0.047
0.992GlnGln: 0.992 ± 0.028
2.166GlnArg: 2.166 ± 0.041
1.814GlnSer: 1.814 ± 0.034
1.584GlnThr: 1.584 ± 0.031
2.351GlnVal: 2.351 ± 0.042
0.345GlnTrp: 0.345 ± 0.018
0.528GlnTyr: 0.528 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
8.937ArgAla: 8.937 ± 0.078
0.469ArgCys: 0.469 ± 0.02
4.273ArgAsp: 4.273 ± 0.054
4.186ArgGlu: 4.186 ± 0.065
2.75ArgPhe: 2.75 ± 0.046
5.068ArgGly: 5.068 ± 0.062
1.644ArgHis: 1.644 ± 0.034
4.307ArgIle: 4.307 ± 0.052
2.121ArgLys: 2.121 ± 0.038
8.39ArgLeu: 8.39 ± 0.095
1.986ArgMet: 1.986 ± 0.038
1.848ArgAsn: 1.848 ± 0.038
3.742ArgPro: 3.742 ± 0.049
2.562ArgGln: 2.562 ± 0.037
5.462ArgArg: 5.462 ± 0.072
3.506ArgSer: 3.506 ± 0.055
2.648ArgThr: 2.648 ± 0.048
4.716ArgVal: 4.716 ± 0.059
0.951ArgTrp: 0.951 ± 0.026
1.559ArgTyr: 1.559 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.268SerAla: 6.268 ± 0.072
0.465SerCys: 0.465 ± 0.019
3.194SerAsp: 3.194 ± 0.052
2.904SerGlu: 2.904 ± 0.048
2.26SerPhe: 2.26 ± 0.041
6.033SerGly: 6.033 ± 0.079
1.167SerHis: 1.167 ± 0.031
2.298SerIle: 2.298 ± 0.039
1.336SerLys: 1.336 ± 0.038
5.574SerLeu: 5.574 ± 0.073
1.174SerMet: 1.174 ± 0.03
1.215SerAsn: 1.215 ± 0.031
2.795SerPro: 2.795 ± 0.045
1.649SerGln: 1.649 ± 0.032
3.708SerArg: 3.708 ± 0.055
2.636SerSer: 2.636 ± 0.045
2.507SerThr: 2.507 ± 0.045
3.916SerVal: 3.916 ± 0.055
0.824SerTrp: 0.824 ± 0.025
1.29SerTyr: 1.29 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
6.417ThrAla: 6.417 ± 0.072
0.474ThrCys: 0.474 ± 0.019
2.87ThrAsp: 2.87 ± 0.048
2.928ThrGlu: 2.928 ± 0.046
1.827ThrPhe: 1.827 ± 0.039
5.832ThrGly: 5.832 ± 0.077
1.045ThrHis: 1.045 ± 0.027
2.613ThrIle: 2.613 ± 0.051
1.237ThrLys: 1.237 ± 0.032
5.714ThrLeu: 5.714 ± 0.065
1.206ThrMet: 1.206 ± 0.032
1.147ThrAsn: 1.147 ± 0.031
3.321ThrPro: 3.321 ± 0.052
1.314ThrGln: 1.314 ± 0.032
3.738ThrArg: 3.738 ± 0.055
2.731ThrSer: 2.731 ± 0.044
2.687ThrThr: 2.687 ± 0.047
3.895ThrVal: 3.895 ± 0.047
0.744ThrTrp: 0.744 ± 0.025
1.026ThrTyr: 1.026 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
8.705ValAla: 8.705 ± 0.082
0.57ValCys: 0.57 ± 0.021
3.487ValAsp: 3.487 ± 0.05
3.893ValGlu: 3.893 ± 0.051
2.694ValPhe: 2.694 ± 0.045
4.698ValGly: 4.698 ± 0.068
1.128ValHis: 1.128 ± 0.026
4.596ValIle: 4.596 ± 0.057
2.09ValLys: 2.09 ± 0.041
7.601ValLeu: 7.601 ± 0.086
2.136ValMet: 2.136 ± 0.041
1.875ValAsn: 1.875 ± 0.034
3.466ValPro: 3.466 ± 0.047
2.011ValGln: 2.011 ± 0.039
3.934ValArg: 3.934 ± 0.054
4.305ValSer: 4.305 ± 0.052
4.951ValThr: 4.951 ± 0.06
5.321ValVal: 5.321 ± 0.066
0.973ValTrp: 0.973 ± 0.028
1.284ValTyr: 1.284 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.428TrpAla: 1.428 ± 0.034
0.141TrpCys: 0.141 ± 0.011
0.754TrpAsp: 0.754 ± 0.022
0.681TrpGlu: 0.681 ± 0.019
0.607TrpPhe: 0.607 ± 0.022
1.053TrpGly: 1.053 ± 0.027
0.362TrpHis: 0.362 ± 0.017
0.779TrpIle: 0.779 ± 0.025
0.506TrpLys: 0.506 ± 0.021
1.899TrpLeu: 1.899 ± 0.036
0.393TrpMet: 0.393 ± 0.017
0.438TrpAsn: 0.438 ± 0.019
0.783TrpPro: 0.783 ± 0.023
0.663TrpGln: 0.663 ± 0.022
1.154TrpArg: 1.154 ± 0.031
0.869TrpSer: 0.869 ± 0.023
0.772TrpThr: 0.772 ± 0.022
0.84TrpVal: 0.84 ± 0.027
0.241TrpTrp: 0.241 ± 0.014
0.301TrpTyr: 0.301 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.347TyrAla: 2.347 ± 0.045
0.212TyrCys: 0.212 ± 0.011
1.44TyrAsp: 1.44 ± 0.034
1.142TyrGlu: 1.142 ± 0.029
0.774TyrPhe: 0.774 ± 0.023
2.019TyrGly: 2.019 ± 0.045
0.454TyrHis: 0.454 ± 0.016
0.948TyrIle: 0.948 ± 0.022
0.502TyrLys: 0.502 ± 0.02
2.083TyrLeu: 2.083 ± 0.045
0.43TyrMet: 0.43 ± 0.016
0.598TyrAsn: 0.598 ± 0.022
1.028TyrPro: 1.028 ± 0.028
0.643TyrGln: 0.643 ± 0.024
1.511TyrArg: 1.511 ± 0.031
1.149TyrSer: 1.149 ± 0.031
1.047TyrThr: 1.047 ± 0.029
1.365TyrVal: 1.365 ± 0.035
0.349TyrTrp: 0.349 ± 0.018
0.522TyrTyr: 0.522 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4521 proteins (1432208 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski