Thermofilum pendens (strain DSM 2475 / Hrk 5)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Thermofilales; Thermofilaceae; Thermofilum; Thermofilum pendens

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1876 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1RW59|A1RW59_THEPD Uncharacterized protein OS=Thermofilum pendens (strain DSM 2475 / Hrk 5) OX=368408 GN=Tpen_0029 PE=4 SV=1
MM1 pKa = 7.36SVTTTVEE8 pKa = 3.92KK9 pKa = 10.24TRR11 pKa = 11.84EE12 pKa = 3.71QIKK15 pKa = 9.92RR16 pKa = 11.84VRR18 pKa = 11.84EE19 pKa = 4.21LYY21 pKa = 10.51RR22 pKa = 11.84GAQLKK27 pKa = 10.37KK28 pKa = 8.18VDD30 pKa = 3.56SPVRR34 pKa = 11.84YY35 pKa = 9.54VRR37 pKa = 11.84VYY39 pKa = 10.31LAAPEE44 pKa = 4.66HH45 pKa = 6.68IYY47 pKa = 10.81DD48 pKa = 3.73YY49 pKa = 11.51VLTFGHH55 pKa = 7.52DD56 pKa = 3.52YY57 pKa = 11.74AEE59 pKa = 4.91DD60 pKa = 3.28KK61 pKa = 10.82TFAIVHH67 pKa = 5.84LRR69 pKa = 11.84EE70 pKa = 3.54IWEE73 pKa = 4.41GEE75 pKa = 3.9FFEE78 pKa = 6.54DD79 pKa = 4.83IIEE82 pKa = 4.13EE83 pKa = 3.99HH84 pKa = 7.43DD85 pKa = 3.72EE86 pKa = 4.2LKK88 pKa = 10.68VWEE91 pKa = 4.47GAEE94 pKa = 4.09GDD96 pKa = 4.9GVILWFLGYY105 pKa = 9.83VGEE108 pKa = 5.08VIEE111 pKa = 4.86LWSDD115 pKa = 3.43LDD117 pKa = 3.24IHH119 pKa = 7.05VMVEE123 pKa = 3.72FHH125 pKa = 7.42EE126 pKa = 4.59DD127 pKa = 3.14FEE129 pKa = 6.51DD130 pKa = 3.38IVNYY134 pKa = 10.42YY135 pKa = 10.51LGAEE139 pKa = 4.19EE140 pKa = 4.59DD141 pKa = 3.71WEE143 pKa = 4.2EE144 pKa = 5.12EE145 pKa = 4.15EE146 pKa = 4.48EE147 pKa = 4.13DD148 pKa = 3.41WEE150 pKa = 5.04GFCEE154 pKa = 4.22EE155 pKa = 4.34

Molecular weight: 18.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1RXF9|A1RXF9_THEPD Uncharacterized protein OS=Thermofilum pendens (strain DSM 2475 / Hrk 5) OX=368408 GN=Tpen_0480 PE=4 SV=1
MM1 pKa = 7.51AHH3 pKa = 6.89FKK5 pKa = 10.66PLGKK9 pKa = 10.13KK10 pKa = 9.72LRR12 pKa = 11.84LAAAVRR18 pKa = 11.84SNEE21 pKa = 3.78QVPIWVISKK30 pKa = 8.6TLGKK34 pKa = 10.04VRR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.44PRR40 pKa = 11.84RR41 pKa = 11.84NWRR44 pKa = 11.84RR45 pKa = 11.84SRR47 pKa = 11.84MQLL50 pKa = 3.09

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1876

0

1876

544134

31

1711

290.1

32.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.293 ± 0.07

0.759 ± 0.022

4.118 ± 0.037

7.851 ± 0.071

3.736 ± 0.036

7.958 ± 0.057

1.426 ± 0.02

4.545 ± 0.046

5.031 ± 0.051

11.618 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.805 ± 0.023

2.252 ± 0.033

4.75 ± 0.04

1.821 ± 0.03

7.241 ± 0.067

6.33 ± 0.046

4.118 ± 0.043

10.249 ± 0.054

1.242 ± 0.024

3.856 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski