Amino acid dipepetide frequency for Thermofilum pendens (strain DSM 2475 / Hrk 5)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.09AlaAla: 8.09 ± 0.197
0.663AlaCys: 0.663 ± 0.04
2.968AlaAsp: 2.968 ± 0.073
6.642AlaGlu: 6.642 ± 0.128
3.778AlaPhe: 3.778 ± 0.091
7.426AlaGly: 7.426 ± 0.133
1.123AlaHis: 1.123 ± 0.049
3.826AlaIle: 3.826 ± 0.089
4.205AlaLys: 4.205 ± 0.092
12.756AlaLeu: 12.756 ± 0.209
1.897AlaMet: 1.897 ± 0.064
1.446AlaAsn: 1.446 ± 0.057
3.501AlaPro: 3.501 ± 0.093
1.773AlaGln: 1.773 ± 0.059
7.809AlaArg: 7.809 ± 0.149
6.599AlaSer: 6.599 ± 0.12
3.988AlaThr: 3.988 ± 0.092
9.797AlaVal: 9.797 ± 0.166
1.195AlaTrp: 1.195 ± 0.053
3.444AlaTyr: 3.444 ± 0.08
0.0AlaXaa: 0.0 ± 0.0
Cys
0.566CysAla: 0.566 ± 0.034
0.079CysCys: 0.079 ± 0.012
0.289CysAsp: 0.289 ± 0.022
0.395CysGlu: 0.395 ± 0.03
0.27CysPhe: 0.27 ± 0.022
1.025CysGly: 1.025 ± 0.054
0.108CysHis: 0.108 ± 0.014
0.292CysIle: 0.292 ± 0.025
0.259CysLys: 0.259 ± 0.022
0.636CysLeu: 0.636 ± 0.032
0.147CysMet: 0.147 ± 0.019
0.2CysAsn: 0.2 ± 0.021
0.739CysPro: 0.739 ± 0.049
0.112CysGln: 0.112 ± 0.015
0.636CysArg: 0.636 ± 0.04
0.5CysSer: 0.5 ± 0.035
0.377CysThr: 0.377 ± 0.028
0.595CysVal: 0.595 ± 0.036
0.101CysTrp: 0.101 ± 0.013
0.261CysTyr: 0.261 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
4.01AspAla: 4.01 ± 0.096
0.272AspCys: 0.272 ± 0.026
1.448AspAsp: 1.448 ± 0.056
3.589AspGlu: 3.589 ± 0.094
1.845AspPhe: 1.845 ± 0.053
2.917AspGly: 2.917 ± 0.074
0.496AspHis: 0.496 ± 0.027
2.141AspIle: 2.141 ± 0.07
1.83AspLys: 1.83 ± 0.067
4.381AspLeu: 4.381 ± 0.085
0.706AspMet: 0.706 ± 0.039
0.757AspAsn: 0.757 ± 0.038
2.97AspPro: 2.97 ± 0.083
0.559AspGln: 0.559 ± 0.036
2.123AspArg: 2.123 ± 0.058
2.231AspSer: 2.231 ± 0.065
1.588AspThr: 1.588 ± 0.061
4.841AspVal: 4.841 ± 0.091
0.687AspTrp: 0.687 ± 0.032
1.788AspTyr: 1.788 ± 0.058
0.0AspXaa: 0.0 ± 0.0
Glu
7.695GluAla: 7.695 ± 0.136
0.513GluCys: 0.513 ± 0.034
3.418GluAsp: 3.418 ± 0.086
8.599GluGlu: 8.599 ± 0.2
2.31GluPhe: 2.31 ± 0.066
5.706GluGly: 5.706 ± 0.11
1.084GluHis: 1.084 ± 0.049
3.821GluIle: 3.821 ± 0.115
6.052GluLys: 6.052 ± 0.117
8.546GluLeu: 8.546 ± 0.167
1.296GluMet: 1.296 ± 0.046
2.012GluAsn: 2.012 ± 0.066
2.782GluPro: 2.782 ± 0.081
1.191GluGln: 1.191 ± 0.051
5.741GluArg: 5.741 ± 0.128
3.383GluSer: 3.383 ± 0.082
2.452GluThr: 2.452 ± 0.068
8.18GluVal: 8.18 ± 0.134
0.95GluTrp: 0.95 ± 0.045
2.779GluTyr: 2.779 ± 0.073
0.0GluXaa: 0.0 ± 0.0
Phe
3.279PheAla: 3.279 ± 0.094
0.244PheCys: 0.244 ± 0.024
1.724PheAsp: 1.724 ± 0.056
2.72PheGlu: 2.72 ± 0.072
1.784PhePhe: 1.784 ± 0.068
2.452PheGly: 2.452 ± 0.074
0.533PheHis: 0.533 ± 0.03
1.652PheIle: 1.652 ± 0.06
1.709PheLys: 1.709 ± 0.053
4.6PheLeu: 4.6 ± 0.102
0.685PheMet: 0.685 ± 0.036
1.071PheAsn: 1.071 ± 0.046
1.586PhePro: 1.586 ± 0.05
0.763PheGln: 0.763 ± 0.038
2.36PheArg: 2.36 ± 0.066
2.86PheSer: 2.86 ± 0.085
2.047PheThr: 2.047 ± 0.072
3.304PheVal: 3.304 ± 0.082
0.414PheTrp: 0.414 ± 0.027
1.575PheTyr: 1.575 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
6.706GlyAla: 6.706 ± 0.123
0.72GlyCys: 0.72 ± 0.039
3.565GlyAsp: 3.565 ± 0.085
6.237GlyGlu: 6.237 ± 0.131
3.802GlyPhe: 3.802 ± 0.09
6.248GlyGly: 6.248 ± 0.128
1.009GlyHis: 1.009 ± 0.044
3.784GlyIle: 3.784 ± 0.091
4.477GlyLys: 4.477 ± 0.091
8.555GlyLeu: 8.555 ± 0.163
1.612GlyMet: 1.612 ± 0.049
1.882GlyAsn: 1.882 ± 0.06
2.297GlyPro: 2.297 ± 0.07
1.274GlyGln: 1.274 ± 0.057
5.747GlyArg: 5.747 ± 0.116
5.03GlySer: 5.03 ± 0.113
3.005GlyThr: 3.005 ± 0.088
8.687GlyVal: 8.687 ± 0.13
1.323GlyTrp: 1.323 ± 0.062
3.62GlyTyr: 3.62 ± 0.089
0.0GlyXaa: 0.0 ± 0.0
His
1.417HisAla: 1.417 ± 0.057
0.154HisCys: 0.154 ± 0.019
0.605HisAsp: 0.605 ± 0.043
0.923HisGlu: 0.923 ± 0.043
0.572HisPhe: 0.572 ± 0.037
1.375HisGly: 1.375 ± 0.054
0.314HisHis: 0.314 ± 0.028
0.625HisIle: 0.625 ± 0.035
0.425HisLys: 0.425 ± 0.029
1.443HisLeu: 1.443 ± 0.049
0.281HisMet: 0.281 ± 0.019
0.298HisAsn: 0.298 ± 0.024
0.952HisPro: 0.952 ± 0.042
0.232HisGln: 0.232 ± 0.02
0.937HisArg: 0.937 ± 0.041
0.818HisSer: 0.818 ± 0.042
0.507HisThr: 0.507 ± 0.033
1.652HisVal: 1.652 ± 0.057
0.195HisTrp: 0.195 ± 0.021
0.538HisTyr: 0.538 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
4.945IleAla: 4.945 ± 0.108
0.274IleCys: 0.274 ± 0.023
2.268IleAsp: 2.268 ± 0.075
3.347IleGlu: 3.347 ± 0.092
1.551IlePhe: 1.551 ± 0.06
3.304IleGly: 3.304 ± 0.085
0.79IleHis: 0.79 ± 0.039
2.185IleIle: 2.185 ± 0.075
1.948IleLys: 1.948 ± 0.073
5.173IleLeu: 5.173 ± 0.101
0.748IleMet: 0.748 ± 0.037
1.121IleAsn: 1.121 ± 0.048
2.687IlePro: 2.687 ± 0.075
0.801IleGln: 0.801 ± 0.038
2.711IleArg: 2.711 ± 0.073
2.687IleSer: 2.687 ± 0.07
2.003IleThr: 2.003 ± 0.08
4.797IleVal: 4.797 ± 0.104
0.38IleTrp: 0.38 ± 0.028
1.729IleTyr: 1.729 ± 0.064
0.0IleXaa: 0.0 ± 0.0
Lys
5.192LysAla: 5.192 ± 0.106
0.456LysCys: 0.456 ± 0.04
1.716LysAsp: 1.716 ± 0.062
3.492LysGlu: 3.492 ± 0.094
1.25LysPhe: 1.25 ± 0.063
3.926LysGly: 3.926 ± 0.086
0.853LysHis: 0.853 ± 0.046
2.632LysIle: 2.632 ± 0.084
2.819LysLys: 2.819 ± 0.091
5.478LysLeu: 5.478 ± 0.105
0.921LysMet: 0.921 ± 0.042
1.184LysAsn: 1.184 ± 0.048
2.981LysPro: 2.981 ± 0.077
0.882LysGln: 0.882 ± 0.043
3.668LysArg: 3.668 ± 0.094
2.714LysSer: 2.714 ± 0.078
2.483LysThr: 2.483 ± 0.057
5.186LysVal: 5.186 ± 0.108
0.502LysTrp: 0.502 ± 0.029
1.979LysTyr: 1.979 ± 0.062
0.0LysXaa: 0.0 ± 0.0
Leu
12.405LeuAla: 12.405 ± 0.189
0.737LeuCys: 0.737 ± 0.036
5.028LeuAsp: 5.028 ± 0.102
9.433LeuGlu: 9.433 ± 0.16
3.554LeuPhe: 3.554 ± 0.088
9.534LeuGly: 9.534 ± 0.164
1.571LeuHis: 1.571 ± 0.062
4.038LeuIle: 4.038 ± 0.092
6.074LeuLys: 6.074 ± 0.118
13.682LeuLeu: 13.682 ± 0.246
1.739LeuMet: 1.739 ± 0.061
2.505LeuAsn: 2.505 ± 0.076
5.104LeuPro: 5.104 ± 0.098
2.235LeuGln: 2.235 ± 0.063
9.292LeuArg: 9.292 ± 0.157
8.625LeuSer: 8.625 ± 0.153
4.379LeuThr: 4.379 ± 0.089
10.909LeuVal: 10.909 ± 0.151
1.209LeuTrp: 1.209 ± 0.057
4.128LeuTyr: 4.128 ± 0.088
0.0LeuXaa: 0.0 ± 0.0
Met
1.509MetAla: 1.509 ± 0.059
0.14MetCys: 0.14 ± 0.018
0.695MetAsp: 0.695 ± 0.038
1.169MetGlu: 1.169 ± 0.047
0.597MetPhe: 0.597 ± 0.035
1.621MetGly: 1.621 ± 0.054
0.333MetHis: 0.333 ± 0.025
0.733MetIle: 0.733 ± 0.034
0.991MetLys: 0.991 ± 0.042
2.215MetLeu: 2.215 ± 0.067
0.309MetMet: 0.309 ± 0.023
0.487MetAsn: 0.487 ± 0.033
0.948MetPro: 0.948 ± 0.041
0.371MetGln: 0.371 ± 0.027
1.309MetArg: 1.309 ± 0.053
1.461MetSer: 1.461 ± 0.052
0.779MetThr: 0.779 ± 0.039
1.564MetVal: 1.564 ± 0.056
0.228MetTrp: 0.228 ± 0.019
0.588MetTyr: 0.588 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
2.178AsnAla: 2.178 ± 0.074
0.237AsnCys: 0.237 ± 0.025
0.757AsnAsp: 0.757 ± 0.042
1.211AsnGlu: 1.211 ± 0.051
0.932AsnPhe: 0.932 ± 0.046
1.691AsnGly: 1.691 ± 0.071
0.298AsnHis: 0.298 ± 0.028
1.345AsnIle: 1.345 ± 0.053
0.858AsnLys: 0.858 ± 0.041
2.51AsnLeu: 2.51 ± 0.07
0.437AsnMet: 0.437 ± 0.028
0.583AsnAsn: 0.583 ± 0.035
1.801AsnPro: 1.801 ± 0.056
0.316AsnGln: 0.316 ± 0.024
1.127AsnArg: 1.127 ± 0.045
1.103AsnSer: 1.103 ± 0.045
1.13AsnThr: 1.13 ± 0.049
2.694AsnVal: 2.694 ± 0.074
0.347AsnTrp: 0.347 ± 0.031
0.969AsnTyr: 0.969 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
3.589ProAla: 3.589 ± 0.082
0.368ProCys: 0.368 ± 0.027
2.08ProAsp: 2.08 ± 0.073
4.05ProGlu: 4.05 ± 0.08
1.751ProPhe: 1.751 ± 0.061
4.622ProGly: 4.622 ± 0.104
0.746ProHis: 0.746 ± 0.036
1.781ProIle: 1.781 ± 0.061
1.648ProLys: 1.648 ± 0.066
5.227ProLeu: 5.227 ± 0.112
0.807ProMet: 0.807 ± 0.034
0.919ProAsn: 0.919 ± 0.041
3.031ProPro: 3.031 ± 0.093
1.16ProGln: 1.16 ± 0.048
3.495ProArg: 3.495 ± 0.088
3.483ProSer: 3.483 ± 0.097
1.81ProThr: 1.81 ± 0.064
4.514ProVal: 4.514 ± 0.092
0.651ProTrp: 0.651 ± 0.038
1.764ProTyr: 1.764 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
1.882GlnAla: 1.882 ± 0.055
0.114GlnCys: 0.114 ± 0.016
0.588GlnAsp: 0.588 ± 0.035
1.297GlnGlu: 1.297 ± 0.056
0.555GlnPhe: 0.555 ± 0.032
1.448GlnGly: 1.448 ± 0.051
0.241GlnHis: 0.241 ± 0.019
0.796GlnIle: 0.796 ± 0.045
0.889GlnLys: 0.889 ± 0.041
2.119GlnLeu: 2.119 ± 0.065
0.397GlnMet: 0.397 ± 0.026
0.408GlnAsn: 0.408 ± 0.027
1.033GlnPro: 1.033 ± 0.049
0.621GlnGln: 0.621 ± 0.043
1.472GlnArg: 1.472 ± 0.055
0.992GlnSer: 0.992 ± 0.043
0.658GlnThr: 0.658 ± 0.032
1.882GlnVal: 1.882 ± 0.064
0.248GlnTrp: 0.248 ± 0.023
0.572GlnTyr: 0.572 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
5.756ArgAla: 5.756 ± 0.106
0.617ArgCys: 0.617 ± 0.038
2.944ArgAsp: 2.944 ± 0.072
6.895ArgGlu: 6.895 ± 0.144
2.433ArgPhe: 2.433 ± 0.069
6.454ArgGly: 6.454 ± 0.134
1.038ArgHis: 1.038 ± 0.053
3.964ArgIle: 3.964 ± 0.1
3.541ArgLys: 3.541 ± 0.086
8.731ArgLeu: 8.731 ± 0.147
1.472ArgMet: 1.472 ± 0.051
1.547ArgAsn: 1.547 ± 0.056
2.325ArgPro: 2.325 ± 0.07
1.16ArgGln: 1.16 ± 0.047
6.217ArgArg: 6.217 ± 0.147
3.723ArgSer: 3.723 ± 0.092
2.215ArgThr: 2.215 ± 0.073
8.18ArgVal: 8.18 ± 0.143
0.812ArgTrp: 0.812 ± 0.046
2.385ArgTyr: 2.385 ± 0.072
0.0ArgXaa: 0.0 ± 0.0
Ser
5.058SerAla: 5.058 ± 0.106
0.458SerCys: 0.458 ± 0.028
2.288SerAsp: 2.288 ± 0.059
4.175SerGlu: 4.175 ± 0.09
2.555SerPhe: 2.555 ± 0.071
5.232SerGly: 5.232 ± 0.116
0.827SerHis: 0.827 ± 0.041
3.176SerIle: 3.176 ± 0.083
2.97SerLys: 2.97 ± 0.08
8.29SerLeu: 8.29 ± 0.15
1.354SerMet: 1.354 ± 0.061
1.239SerAsn: 1.239 ± 0.05
3.328SerPro: 3.328 ± 0.093
1.349SerGln: 1.349 ± 0.047
4.986SerArg: 4.986 ± 0.102
4.379SerSer: 4.379 ± 0.104
2.997SerThr: 2.997 ± 0.078
5.418SerVal: 5.418 ± 0.115
0.851SerTrp: 0.851 ± 0.041
2.373SerTyr: 2.373 ± 0.065
0.0SerXaa: 0.0 ± 0.0
Thr
3.567ThrAla: 3.567 ± 0.083
0.333ThrCys: 0.333 ± 0.024
1.279ThrAsp: 1.279 ± 0.054
1.957ThrGlu: 1.957 ± 0.059
1.623ThrPhe: 1.623 ± 0.063
3.971ThrGly: 3.971 ± 0.104
0.763ThrHis: 0.763 ± 0.038
2.091ThrIle: 2.091 ± 0.067
1.472ThrLys: 1.472 ± 0.056
5.313ThrLeu: 5.313 ± 0.121
0.713ThrMet: 0.713 ± 0.042
0.869ThrAsn: 0.869 ± 0.035
2.742ThrPro: 2.742 ± 0.073
0.755ThrGln: 0.755 ± 0.045
2.747ThrArg: 2.747 ± 0.075
2.525ThrSer: 2.525 ± 0.073
1.981ThrThr: 1.981 ± 0.087
4.302ThrVal: 4.302 ± 0.11
0.599ThrTrp: 0.599 ± 0.034
1.579ThrTyr: 1.579 ± 0.062
0.0ThrXaa: 0.0 ± 0.0
Val
10.387ValAla: 10.387 ± 0.161
0.768ValCys: 0.768 ± 0.047
5.322ValAsp: 5.322 ± 0.092
8.93ValGlu: 8.93 ± 0.172
4.245ValPhe: 4.245 ± 0.098
6.563ValGly: 6.563 ± 0.117
1.456ValHis: 1.456 ± 0.052
4.216ValIle: 4.216 ± 0.098
6.101ValLys: 6.101 ± 0.124
10.85ValLeu: 10.85 ± 0.154
1.518ValMet: 1.518 ± 0.06
2.702ValAsn: 2.702 ± 0.078
4.35ValPro: 4.35 ± 0.085
1.705ValGln: 1.705 ± 0.055
6.046ValArg: 6.046 ± 0.127
6.658ValSer: 6.658 ± 0.112
4.361ValThr: 4.361 ± 0.094
10.825ValVal: 10.825 ± 0.164
1.003ValTrp: 1.003 ± 0.038
4.484ValTyr: 4.484 ± 0.092
0.0ValXaa: 0.0 ± 0.0
Trp
0.877TrpAla: 0.877 ± 0.04
0.11TrpCys: 0.11 ± 0.016
0.572TrpAsp: 0.572 ± 0.039
0.873TrpGlu: 0.873 ± 0.052
0.47TrpPhe: 0.47 ± 0.032
0.976TrpGly: 0.976 ± 0.045
0.239TrpHis: 0.239 ± 0.021
0.629TrpIle: 0.629 ± 0.03
0.588TrpLys: 0.588 ± 0.035
1.456TrpLeu: 1.456 ± 0.064
0.237TrpMet: 0.237 ± 0.019
0.344TrpAsn: 0.344 ± 0.03
0.404TrpPro: 0.404 ± 0.028
0.279TrpGln: 0.279 ± 0.024
1.143TrpArg: 1.143 ± 0.048
0.877TrpSer: 0.877 ± 0.039
0.5TrpThr: 0.5 ± 0.028
1.202TrpVal: 1.202 ± 0.049
0.193TrpTrp: 0.193 ± 0.018
0.452TrpTyr: 0.452 ± 0.036
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.821TyrAla: 3.821 ± 0.086
0.331TyrCys: 0.331 ± 0.027
1.625TyrAsp: 1.625 ± 0.059
2.575TyrGlu: 2.575 ± 0.07
1.483TyrPhe: 1.483 ± 0.053
3.089TyrGly: 3.089 ± 0.08
0.439TyrHis: 0.439 ± 0.028
1.724TyrIle: 1.724 ± 0.062
1.457TyrLys: 1.457 ± 0.049
4.22TyrLeu: 4.22 ± 0.095
0.768TyrMet: 0.768 ± 0.037
0.95TyrAsn: 0.95 ± 0.044
1.935TyrPro: 1.935 ± 0.062
0.674TyrGln: 0.674 ± 0.035
2.847TyrArg: 2.847 ± 0.074
2.654TyrSer: 2.654 ± 0.071
1.922TyrThr: 1.922 ± 0.063
3.962TyrVal: 3.962 ± 0.078
0.531TyrTrp: 0.531 ± 0.032
1.551TyrTyr: 1.551 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1876 proteins (544134 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski