Amino acid dipepetide frequency for Streptococcus phage TP-778L

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.533AlaAla: 5.533 ± 1.613
0.39AlaCys: 0.39 ± 0.246
4.831AlaAsp: 4.831 ± 0.751
4.598AlaGlu: 4.598 ± 0.577
2.961AlaPhe: 2.961 ± 0.913
5.766AlaGly: 5.766 ± 1.221
0.701AlaHis: 0.701 ± 0.218
5.688AlaIle: 5.688 ± 1.193
5.844AlaLys: 5.844 ± 0.719
6.624AlaLeu: 6.624 ± 0.899
2.338AlaMet: 2.338 ± 0.989
4.286AlaAsn: 4.286 ± 0.572
1.948AlaPro: 1.948 ± 0.5
2.571AlaGln: 2.571 ± 0.752
2.961AlaArg: 2.961 ± 0.549
6.0AlaSer: 6.0 ± 1.376
4.13AlaThr: 4.13 ± 0.574
4.286AlaVal: 4.286 ± 0.807
0.623AlaTrp: 0.623 ± 0.249
3.039AlaTyr: 3.039 ± 0.655
0.0AlaXaa: 0.0 ± 0.0
Cys
0.156CysAla: 0.156 ± 0.112
0.078CysCys: 0.078 ± 0.079
0.545CysAsp: 0.545 ± 0.224
0.545CysGlu: 0.545 ± 0.198
0.234CysPhe: 0.234 ± 0.138
0.468CysGly: 0.468 ± 0.263
0.0CysHis: 0.0 ± 0.0
0.234CysIle: 0.234 ± 0.128
0.857CysLys: 0.857 ± 0.348
0.312CysLeu: 0.312 ± 0.169
0.0CysMet: 0.0 ± 0.0
0.39CysAsn: 0.39 ± 0.198
0.156CysPro: 0.156 ± 0.113
0.078CysGln: 0.078 ± 0.093
0.156CysArg: 0.156 ± 0.112
0.312CysSer: 0.312 ± 0.176
0.078CysThr: 0.078 ± 0.067
0.234CysVal: 0.234 ± 0.144
0.078CysTrp: 0.078 ± 0.092
0.623CysTyr: 0.623 ± 0.263
0.0CysXaa: 0.0 ± 0.0
Asp
3.662AspAla: 3.662 ± 0.393
0.623AspCys: 0.623 ± 0.297
3.896AspAsp: 3.896 ± 0.492
4.208AspGlu: 4.208 ± 0.894
3.039AspPhe: 3.039 ± 0.414
6.779AspGly: 6.779 ± 1.342
0.779AspHis: 0.779 ± 0.307
3.974AspIle: 3.974 ± 0.663
5.377AspLys: 5.377 ± 0.719
4.442AspLeu: 4.442 ± 0.725
1.325AspMet: 1.325 ± 0.259
3.273AspAsn: 3.273 ± 0.576
1.091AspPro: 1.091 ± 0.366
1.558AspGln: 1.558 ± 0.366
2.338AspArg: 2.338 ± 0.302
4.52AspSer: 4.52 ± 0.683
3.429AspThr: 3.429 ± 0.458
3.429AspVal: 3.429 ± 0.486
1.169AspTrp: 1.169 ± 0.344
3.273AspTyr: 3.273 ± 0.506
0.0AspXaa: 0.0 ± 0.0
Glu
4.598GluAla: 4.598 ± 0.715
0.156GluCys: 0.156 ± 0.13
3.039GluAsp: 3.039 ± 0.391
4.052GluGlu: 4.052 ± 0.762
2.727GluPhe: 2.727 ± 0.443
3.662GluGly: 3.662 ± 0.533
1.091GluHis: 1.091 ± 0.335
5.065GluIle: 5.065 ± 0.806
4.286GluLys: 4.286 ± 0.91
7.87GluLeu: 7.87 ± 1.262
2.026GluMet: 2.026 ± 0.374
3.662GluAsn: 3.662 ± 0.589
1.481GluPro: 1.481 ± 0.47
2.883GluGln: 2.883 ± 0.558
3.74GluArg: 3.74 ± 0.858
3.195GluSer: 3.195 ± 0.535
3.429GluThr: 3.429 ± 0.643
4.753GluVal: 4.753 ± 0.66
1.091GluTrp: 1.091 ± 0.308
3.039GluTyr: 3.039 ± 0.547
0.0GluXaa: 0.0 ± 0.0
Phe
2.26PheAla: 2.26 ± 0.431
0.156PheCys: 0.156 ± 0.091
2.649PheAsp: 2.649 ± 0.469
3.273PheGlu: 3.273 ± 0.471
1.169PhePhe: 1.169 ± 0.261
3.117PheGly: 3.117 ± 0.687
0.39PheHis: 0.39 ± 0.143
2.805PheIle: 2.805 ± 0.348
4.286PheLys: 4.286 ± 0.579
1.87PheLeu: 1.87 ± 0.412
1.013PheMet: 1.013 ± 0.226
3.429PheAsn: 3.429 ± 0.437
0.701PhePro: 0.701 ± 0.211
0.935PheGln: 0.935 ± 0.28
1.403PheArg: 1.403 ± 0.266
3.662PheSer: 3.662 ± 0.527
2.961PheThr: 2.961 ± 0.575
2.26PheVal: 2.26 ± 0.452
0.468PheTrp: 0.468 ± 0.167
1.247PheTyr: 1.247 ± 0.33
0.0PheXaa: 0.0 ± 0.0
Gly
5.065GlyAla: 5.065 ± 0.733
0.312GlyCys: 0.312 ± 0.201
3.351GlyAsp: 3.351 ± 0.411
3.273GlyGlu: 3.273 ± 0.431
3.429GlyPhe: 3.429 ± 0.505
3.507GlyGly: 3.507 ± 0.562
1.091GlyHis: 1.091 ± 0.334
6.39GlyIle: 6.39 ± 1.594
5.611GlyLys: 5.611 ± 0.708
5.611GlyLeu: 5.611 ± 0.817
2.104GlyMet: 2.104 ± 0.7
4.13GlyAsn: 4.13 ± 0.437
1.169GlyPro: 1.169 ± 0.473
3.195GlyGln: 3.195 ± 0.516
3.74GlyArg: 3.74 ± 0.786
4.208GlySer: 4.208 ± 0.797
5.455GlyThr: 5.455 ± 1.007
3.974GlyVal: 3.974 ± 0.528
1.169GlyTrp: 1.169 ± 0.309
3.117GlyTyr: 3.117 ± 0.449
0.0GlyXaa: 0.0 ± 0.0
His
0.701HisAla: 0.701 ± 0.246
0.156HisCys: 0.156 ± 0.098
1.013HisAsp: 1.013 ± 0.269
0.779HisGlu: 0.779 ± 0.341
0.545HisPhe: 0.545 ± 0.186
0.857HisGly: 0.857 ± 0.305
0.312HisHis: 0.312 ± 0.157
0.935HisIle: 0.935 ± 0.231
1.013HisLys: 1.013 ± 0.232
0.857HisLeu: 0.857 ± 0.282
0.156HisMet: 0.156 ± 0.102
0.857HisAsn: 0.857 ± 0.232
0.234HisPro: 0.234 ± 0.111
0.312HisGln: 0.312 ± 0.167
0.39HisArg: 0.39 ± 0.169
0.779HisSer: 0.779 ± 0.283
0.701HisThr: 0.701 ± 0.164
0.857HisVal: 0.857 ± 0.339
0.078HisTrp: 0.078 ± 0.079
0.857HisTyr: 0.857 ± 0.232
0.0HisXaa: 0.0 ± 0.0
Ile
5.844IleAla: 5.844 ± 1.185
0.545IleCys: 0.545 ± 0.248
5.377IleAsp: 5.377 ± 0.724
4.208IleGlu: 4.208 ± 0.617
1.792IlePhe: 1.792 ± 0.422
5.221IleGly: 5.221 ± 0.962
1.169IleHis: 1.169 ± 0.263
3.507IleIle: 3.507 ± 0.756
4.675IleLys: 4.675 ± 0.477
3.507IleLeu: 3.507 ± 0.489
1.714IleMet: 1.714 ± 0.406
3.74IleAsn: 3.74 ± 0.709
2.883IlePro: 2.883 ± 0.522
3.429IleGln: 3.429 ± 0.403
2.883IleArg: 2.883 ± 0.525
5.533IleSer: 5.533 ± 1.315
4.598IleThr: 4.598 ± 0.716
4.13IleVal: 4.13 ± 0.814
0.701IleTrp: 0.701 ± 0.279
2.961IleTyr: 2.961 ± 0.694
0.0IleXaa: 0.0 ± 0.0
Lys
6.779LysAla: 6.779 ± 0.792
0.39LysCys: 0.39 ± 0.214
3.896LysAsp: 3.896 ± 0.571
6.468LysGlu: 6.468 ± 0.882
2.182LysPhe: 2.182 ± 0.379
5.611LysGly: 5.611 ± 0.789
1.013LysHis: 1.013 ± 0.337
4.987LysIle: 4.987 ± 1.098
5.611LysLys: 5.611 ± 1.123
7.013LysLeu: 7.013 ± 0.855
1.481LysMet: 1.481 ± 0.292
4.442LysAsn: 4.442 ± 0.762
2.805LysPro: 2.805 ± 0.38
2.649LysGln: 2.649 ± 0.451
4.753LysArg: 4.753 ± 0.736
4.442LysSer: 4.442 ± 0.544
5.688LysThr: 5.688 ± 0.694
4.208LysVal: 4.208 ± 0.612
1.013LysTrp: 1.013 ± 0.247
3.195LysTyr: 3.195 ± 0.765
0.0LysXaa: 0.0 ± 0.0
Leu
6.624LeuAla: 6.624 ± 0.783
0.39LeuCys: 0.39 ± 0.156
5.143LeuAsp: 5.143 ± 0.683
6.39LeuGlu: 6.39 ± 0.892
2.961LeuPhe: 2.961 ± 0.46
5.065LeuGly: 5.065 ± 0.929
0.779LeuHis: 0.779 ± 0.291
4.052LeuIle: 4.052 ± 0.548
6.156LeuLys: 6.156 ± 0.788
5.143LeuLeu: 5.143 ± 0.685
1.714LeuMet: 1.714 ± 0.314
5.221LeuAsn: 5.221 ± 0.56
2.805LeuPro: 2.805 ± 0.472
2.494LeuGln: 2.494 ± 0.326
2.571LeuArg: 2.571 ± 0.563
5.143LeuSer: 5.143 ± 0.661
6.701LeuThr: 6.701 ± 0.838
4.909LeuVal: 4.909 ± 0.657
0.779LeuTrp: 0.779 ± 0.202
3.117LeuTyr: 3.117 ± 0.598
0.0LeuXaa: 0.0 ± 0.0
Met
2.805MetAla: 2.805 ± 0.728
0.0MetCys: 0.0 ± 0.0
0.857MetAsp: 0.857 ± 0.197
1.403MetGlu: 1.403 ± 0.271
0.779MetPhe: 0.779 ± 0.225
1.247MetGly: 1.247 ± 0.478
0.078MetHis: 0.078 ± 0.067
1.558MetIle: 1.558 ± 0.449
2.182MetLys: 2.182 ± 0.381
1.403MetLeu: 1.403 ± 0.289
1.013MetMet: 1.013 ± 0.463
1.091MetAsn: 1.091 ± 0.248
0.623MetPro: 0.623 ± 0.233
1.325MetGln: 1.325 ± 0.548
1.013MetArg: 1.013 ± 0.25
2.104MetSer: 2.104 ± 0.45
1.325MetThr: 1.325 ± 0.312
1.325MetVal: 1.325 ± 0.371
0.078MetTrp: 0.078 ± 0.072
0.779MetTyr: 0.779 ± 0.396
0.0MetXaa: 0.0 ± 0.0
Asn
3.896AsnAla: 3.896 ± 0.602
0.39AsnCys: 0.39 ± 0.202
3.039AsnAsp: 3.039 ± 0.621
4.208AsnGlu: 4.208 ± 0.812
2.104AsnPhe: 2.104 ± 0.477
5.611AsnGly: 5.611 ± 0.75
1.247AsnHis: 1.247 ± 0.298
3.429AsnIle: 3.429 ± 0.44
4.13AsnLys: 4.13 ± 0.603
3.896AsnLeu: 3.896 ± 0.376
1.169AsnMet: 1.169 ± 0.278
2.805AsnAsn: 2.805 ± 0.573
2.182AsnPro: 2.182 ± 0.524
1.87AsnGln: 1.87 ± 0.414
2.494AsnArg: 2.494 ± 0.638
3.429AsnSer: 3.429 ± 0.503
3.273AsnThr: 3.273 ± 0.637
3.74AsnVal: 3.74 ± 0.45
1.558AsnTrp: 1.558 ± 0.374
2.026AsnTyr: 2.026 ± 0.388
0.0AsnXaa: 0.0 ± 0.0
Pro
1.325ProAla: 1.325 ± 0.303
0.234ProCys: 0.234 ± 0.155
2.26ProAsp: 2.26 ± 0.57
1.636ProGlu: 1.636 ± 0.428
1.013ProPhe: 1.013 ± 0.378
1.636ProGly: 1.636 ± 0.474
0.234ProHis: 0.234 ± 0.145
2.026ProIle: 2.026 ± 0.355
2.883ProLys: 2.883 ± 0.395
1.714ProLeu: 1.714 ± 0.411
0.312ProMet: 0.312 ± 0.153
2.26ProAsn: 2.26 ± 0.59
1.169ProPro: 1.169 ± 0.268
1.247ProGln: 1.247 ± 0.242
1.714ProArg: 1.714 ± 0.353
2.494ProSer: 2.494 ± 0.566
1.87ProThr: 1.87 ± 0.489
2.182ProVal: 2.182 ± 0.422
0.312ProTrp: 0.312 ± 0.128
1.091ProTyr: 1.091 ± 0.28
0.0ProXaa: 0.0 ± 0.0
Gln
3.039GlnAla: 3.039 ± 0.815
0.234GlnCys: 0.234 ± 0.113
2.026GlnAsp: 2.026 ± 0.349
2.649GlnGlu: 2.649 ± 0.577
1.714GlnPhe: 1.714 ± 0.383
2.104GlnGly: 2.104 ± 0.911
0.156GlnHis: 0.156 ± 0.111
2.26GlnIle: 2.26 ± 0.617
2.26GlnLys: 2.26 ± 0.403
3.74GlnLeu: 3.74 ± 0.349
0.623GlnMet: 0.623 ± 0.245
2.26GlnAsn: 2.26 ± 0.297
1.792GlnPro: 1.792 ± 0.488
1.091GlnGln: 1.091 ± 0.323
1.636GlnArg: 1.636 ± 0.359
3.195GlnSer: 3.195 ± 0.631
2.727GlnThr: 2.727 ± 0.47
2.494GlnVal: 2.494 ± 0.427
0.623GlnTrp: 0.623 ± 0.209
1.636GlnTyr: 1.636 ± 0.368
0.0GlnXaa: 0.0 ± 0.0
Arg
3.273ArgAla: 3.273 ± 0.48
0.312ArgCys: 0.312 ± 0.143
2.727ArgAsp: 2.727 ± 0.369
2.649ArgGlu: 2.649 ± 0.564
2.104ArgPhe: 2.104 ± 0.583
2.961ArgGly: 2.961 ± 0.424
0.234ArgHis: 0.234 ± 0.131
2.416ArgIle: 2.416 ± 0.461
3.429ArgLys: 3.429 ± 0.673
3.662ArgLeu: 3.662 ± 0.677
1.558ArgMet: 1.558 ± 0.366
1.87ArgAsn: 1.87 ± 0.379
0.935ArgPro: 0.935 ± 0.236
1.636ArgGln: 1.636 ± 0.361
1.558ArgArg: 1.558 ± 0.329
2.416ArgSer: 2.416 ± 0.325
2.104ArgThr: 2.104 ± 0.476
3.273ArgVal: 3.273 ± 0.657
0.701ArgTrp: 0.701 ± 0.219
2.416ArgTyr: 2.416 ± 0.395
0.0ArgXaa: 0.0 ± 0.0
Ser
6.156SerAla: 6.156 ± 2.037
0.468SerCys: 0.468 ± 0.216
4.442SerAsp: 4.442 ± 0.842
4.208SerGlu: 4.208 ± 0.728
3.507SerPhe: 3.507 ± 0.561
4.909SerGly: 4.909 ± 0.846
0.39SerHis: 0.39 ± 0.171
5.221SerIle: 5.221 ± 0.781
5.143SerLys: 5.143 ± 0.643
5.221SerLeu: 5.221 ± 0.794
1.558SerMet: 1.558 ± 0.32
3.507SerAsn: 3.507 ± 0.457
2.182SerPro: 2.182 ± 0.52
3.74SerGln: 3.74 ± 0.734
2.026SerArg: 2.026 ± 0.345
5.065SerSer: 5.065 ± 1.438
4.364SerThr: 4.364 ± 0.494
5.221SerVal: 5.221 ± 0.917
0.935SerTrp: 0.935 ± 0.346
1.87SerTyr: 1.87 ± 0.414
0.0SerXaa: 0.0 ± 0.0
Thr
4.442ThrAla: 4.442 ± 1.071
0.0ThrCys: 0.0 ± 0.0
4.442ThrAsp: 4.442 ± 0.726
3.117ThrGlu: 3.117 ± 0.654
3.273ThrPhe: 3.273 ± 0.448
3.974ThrGly: 3.974 ± 0.751
1.247ThrHis: 1.247 ± 0.223
6.0ThrIle: 6.0 ± 0.786
5.299ThrLys: 5.299 ± 0.621
6.078ThrLeu: 6.078 ± 0.668
0.857ThrMet: 0.857 ± 0.336
2.961ThrAsn: 2.961 ± 0.441
1.714ThrPro: 1.714 ± 0.366
2.805ThrGln: 2.805 ± 0.559
1.87ThrArg: 1.87 ± 0.372
4.442ThrSer: 4.442 ± 1.002
4.909ThrThr: 4.909 ± 0.585
5.611ThrVal: 5.611 ± 0.754
0.39ThrTrp: 0.39 ± 0.263
2.649ThrTyr: 2.649 ± 0.716
0.0ThrXaa: 0.0 ± 0.0
Val
4.675ValAla: 4.675 ± 0.677
0.156ValCys: 0.156 ± 0.096
5.065ValAsp: 5.065 ± 0.689
5.065ValGlu: 5.065 ± 0.765
2.104ValPhe: 2.104 ± 0.267
4.13ValGly: 4.13 ± 0.695
0.701ValHis: 0.701 ± 0.196
4.364ValIle: 4.364 ± 0.588
6.0ValLys: 6.0 ± 0.7
4.675ValLeu: 4.675 ± 0.458
1.169ValMet: 1.169 ± 0.289
3.351ValAsn: 3.351 ± 0.716
2.026ValPro: 2.026 ± 0.47
2.182ValGln: 2.182 ± 0.384
1.948ValArg: 1.948 ± 0.632
5.533ValSer: 5.533 ± 0.78
4.364ValThr: 4.364 ± 0.523
4.753ValVal: 4.753 ± 0.618
0.857ValTrp: 0.857 ± 0.286
1.403ValTyr: 1.403 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
0.623TrpAla: 0.623 ± 0.219
0.156TrpCys: 0.156 ± 0.094
0.545TrpAsp: 0.545 ± 0.2
1.325TrpGlu: 1.325 ± 0.29
0.857TrpPhe: 0.857 ± 0.363
0.779TrpGly: 0.779 ± 0.215
0.234TrpHis: 0.234 ± 0.15
0.701TrpIle: 0.701 ± 0.286
0.701TrpLys: 0.701 ± 0.162
1.091TrpLeu: 1.091 ± 0.432
0.156TrpMet: 0.156 ± 0.102
0.857TrpAsn: 0.857 ± 0.361
0.312TrpPro: 0.312 ± 0.144
0.468TrpGln: 0.468 ± 0.18
0.623TrpArg: 0.623 ± 0.286
1.403TrpSer: 1.403 ± 0.515
1.013TrpThr: 1.013 ± 0.45
0.779TrpVal: 0.779 ± 0.208
0.312TrpTrp: 0.312 ± 0.219
0.312TrpTyr: 0.312 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.662TyrAla: 3.662 ± 0.646
0.39TyrCys: 0.39 ± 0.183
3.039TyrAsp: 3.039 ± 0.825
1.792TyrGlu: 1.792 ± 0.39
1.558TyrPhe: 1.558 ± 0.323
2.494TyrGly: 2.494 ± 0.44
0.545TyrHis: 0.545 ± 0.173
2.805TyrIle: 2.805 ± 0.555
2.961TyrLys: 2.961 ± 0.498
3.429TyrLeu: 3.429 ± 0.617
0.623TyrMet: 0.623 ± 0.209
2.104TyrAsn: 2.104 ± 0.48
1.403TyrPro: 1.403 ± 0.442
1.792TyrGln: 1.792 ± 0.385
2.416TyrArg: 2.416 ± 0.535
2.26TyrSer: 2.26 ± 0.401
2.961TyrThr: 2.961 ± 0.759
2.104TyrVal: 2.104 ± 0.397
0.312TyrTrp: 0.312 ± 0.173
1.558TyrTyr: 1.558 ± 0.562
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (12834 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski