Aureobasidium subglaciale (strain EXF-2481) (Aureobasidium pullulans var. subglaciale)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Dothideales; Saccotheciaceae; Aureobasidium; Aureobasidium subglaciale

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A074Z0Q4|A0A074Z0Q4_AURSE Atypical serine/threonine protein kinase BUD32 OS=Aureobasidium subglaciale (strain EXF-2481) OX=1043005 GN=AUEXF2481DRAFT_7398 PE=4 SV=1
MM1 pKa = 7.31QKK3 pKa = 10.16RR4 pKa = 11.84SSCTFTSYY12 pKa = 11.75DD13 pKa = 3.81DD14 pKa = 3.94VADD17 pKa = 4.27QIADD21 pKa = 3.77CSSVTLSGITVPAGEE36 pKa = 4.49TLDD39 pKa = 5.08LSDD42 pKa = 4.59AADD45 pKa = 3.86GATITFSGTTTFEE58 pKa = 3.91YY59 pKa = 10.85SEE61 pKa = 4.1WSGPLIRR68 pKa = 11.84AGGKK72 pKa = 9.74GVTITGASGHH82 pKa = 6.25VIDD85 pKa = 5.98GNGAKK90 pKa = 8.86WWDD93 pKa = 3.64GEE95 pKa = 4.32GTNGGKK101 pKa = 8.23TKK103 pKa = 10.58PKK105 pKa = 10.07FFYY108 pKa = 10.56AHH110 pKa = 6.88SLTGNSVIKK119 pKa = 10.46DD120 pKa = 3.72LNIKK124 pKa = 7.56NTPVQAISVQATDD137 pKa = 3.81LTISGLTIDD146 pKa = 5.08NSDD149 pKa = 4.68GDD151 pKa = 5.38AEE153 pKa = 4.54DD154 pKa = 5.38CDD156 pKa = 5.57GEE158 pKa = 4.5ACGHH162 pKa = 5.65NTDD165 pKa = 4.32AFDD168 pKa = 3.47VSEE171 pKa = 4.53SEE173 pKa = 4.13NVVITGCTIKK183 pKa = 11.06NQDD186 pKa = 3.54DD187 pKa = 3.78CLAINSGTGITFSDD201 pKa = 4.0NACSGGHH208 pKa = 6.66GISIGSVGGRR218 pKa = 11.84DD219 pKa = 3.87DD220 pKa = 5.11NDD222 pKa = 3.2VANIIISGNTIKK234 pKa = 10.94DD235 pKa = 3.41SANGLRR241 pKa = 11.84IKK243 pKa = 9.45TVYY246 pKa = 10.47DD247 pKa = 3.17ATGSVKK253 pKa = 10.56NVTYY257 pKa = 10.28TDD259 pKa = 3.49NTISGISDD267 pKa = 3.22YY268 pKa = 11.32GIVVEE273 pKa = 4.61QDD275 pKa = 3.56YY276 pKa = 11.45EE277 pKa = 4.36NGSPTGTPTSGVPITDD293 pKa = 2.77ITFNGVTGTVDD304 pKa = 3.08SDD306 pKa = 3.82AEE308 pKa = 4.16NIYY311 pKa = 10.04ILCASCSGWTWSGVDD326 pKa = 3.11ISGGKK331 pKa = 8.2TSDD334 pKa = 3.02ACEE337 pKa = 4.15GVPSGVSCC345 pKa = 5.13

Molecular weight: 35.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A074YJM6|A0A074YJM6_AURSE Geranylgeranyl transferase type-2 subunit alpha OS=Aureobasidium subglaciale (strain EXF-2481) OX=1043005 GN=AUEXF2481DRAFT_561364 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TNNTIRR36 pKa = 11.84YY37 pKa = 5.73NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10792

0

10792

4879484

49

4935

452.1

50.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.844 ± 0.019

1.261 ± 0.009

5.776 ± 0.018

5.94 ± 0.024

3.721 ± 0.015

6.558 ± 0.026

2.435 ± 0.011

4.905 ± 0.016

4.939 ± 0.022

8.786 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.009

3.78 ± 0.012

5.825 ± 0.025

4.168 ± 0.018

5.83 ± 0.019

8.457 ± 0.034

6.175 ± 0.017

6.102 ± 0.015

1.423 ± 0.009

2.803 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski