Amino acid dipepetide frequency for Phycicoccus sp. Root563

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.381AlaAla: 20.381 ± 0.198	0.93AlaCys: 0.93 ± 0.03	7.953AlaAsp: 7.953 ± 0.084	6.763AlaGlu: 6.763 ± 0.096	3.57AlaPhe: 3.57 ± 0.059	12.486AlaGly: 12.486 ± 0.109	2.72AlaHis: 2.72 ± 0.047	4.169AlaIle: 4.169 ± 0.064	3.123AlaLys: 3.123 ± 0.076	14.006AlaLeu: 14.006 ± 0.156	2.801AlaMet: 2.801 ± 0.043	2.105AlaAsn: 2.105 ± 0.046	6.429AlaPro: 6.429 ± 0.095	3.979AlaGln: 3.979 ± 0.062	9.298AlaArg: 9.298 ± 0.1	6.976AlaSer: 6.976 ± 0.084	8.502AlaThr: 8.502 ± 0.109	12.471AlaVal: 12.471 ± 0.135	1.948AlaTrp: 1.948 ± 0.047	2.548AlaTyr: 2.548 ± 0.049	0.002AlaXaa: 0.002 ± 0.001
Cys	0.856CysAla: 0.856 ± 0.028	0.092CysCys: 0.092 ± 0.009	0.422CysAsp: 0.422 ± 0.019	0.298CysGlu: 0.298 ± 0.016	0.203CysPhe: 0.203 ± 0.014	0.783CysGly: 0.783 ± 0.026	0.18CysHis: 0.18 ± 0.011	0.184CysIle: 0.184 ± 0.013	0.102CysLys: 0.102 ± 0.009	0.585CysLeu: 0.585 ± 0.023	0.106CysMet: 0.106 ± 0.009	0.134CysAsn: 0.134 ± 0.013	0.421CysPro: 0.421 ± 0.019	0.152CysGln: 0.152 ± 0.012	0.493CysArg: 0.493 ± 0.021	0.402CysSer: 0.402 ± 0.019	0.472CysThr: 0.472 ± 0.021	0.589CysVal: 0.589 ± 0.022	0.096CysTrp: 0.096 ± 0.01	0.116CysTyr: 0.116 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.825AspAla: 7.825 ± 0.091	0.361AspCys: 0.361 ± 0.018	4.066AspAsp: 4.066 ± 0.078	4.053AspGlu: 4.053 ± 0.067	1.596AspPhe: 1.596 ± 0.04	6.265AspGly: 6.265 ± 0.075	1.642AspHis: 1.642 ± 0.038	1.857AspIle: 1.857 ± 0.046	1.219AspLys: 1.219 ± 0.04	7.005AspLeu: 7.005 ± 0.084	0.796AspMet: 0.796 ± 0.028	0.974AspAsn: 0.974 ± 0.03	4.337AspPro: 4.337 ± 0.062	1.81AspGln: 1.81 ± 0.041	4.648AspArg: 4.648 ± 0.066	2.718AspSer: 2.718 ± 0.053	3.054AspThr: 3.054 ± 0.049	5.814AspVal: 5.814 ± 0.069	0.919AspTrp: 0.919 ± 0.029	1.125AspTyr: 1.125 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	6.766GluAla: 6.766 ± 0.099	0.312GluCys: 0.312 ± 0.016	2.828GluAsp: 2.828 ± 0.052	2.783GluGlu: 2.783 ± 0.054	1.506GluPhe: 1.506 ± 0.032	4.298GluGly: 4.298 ± 0.063	1.507GluHis: 1.507 ± 0.042	1.918GluIle: 1.918 ± 0.045	1.208GluLys: 1.208 ± 0.034	5.903GluLeu: 5.903 ± 0.081	0.875GluMet: 0.875 ± 0.03	0.873GluAsn: 0.873 ± 0.028	2.769GluPro: 2.769 ± 0.056	2.15GluGln: 2.15 ± 0.047	4.684GluArg: 4.684 ± 0.081	2.641GluSer: 2.641 ± 0.047	2.493GluThr: 2.493 ± 0.047	5.442GluVal: 5.442 ± 0.084	0.697GluTrp: 0.697 ± 0.028	0.883GluTyr: 0.883 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	3.724PheAla: 3.724 ± 0.058	0.227PheCys: 0.227 ± 0.015	2.059PheAsp: 2.059 ± 0.043	1.512PheGlu: 1.512 ± 0.043	0.847PhePhe: 0.847 ± 0.03	3.048PheGly: 3.048 ± 0.056	0.566PheHis: 0.566 ± 0.023	0.847PheIle: 0.847 ± 0.031	0.509PheLys: 0.509 ± 0.026	2.463PheLeu: 2.463 ± 0.057	0.427PheMet: 0.427 ± 0.018	0.591PheAsn: 0.591 ± 0.021	1.304PhePro: 1.304 ± 0.036	0.603PheGln: 0.603 ± 0.023	1.616PheArg: 1.616 ± 0.039	1.584PheSer: 1.584 ± 0.039	1.963PheThr: 1.963 ± 0.042	2.73PheVal: 2.73 ± 0.05	0.433PheTrp: 0.433 ± 0.021	0.552PheTyr: 0.552 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	11.188GlyAla: 11.188 ± 0.116	0.691GlyCys: 0.691 ± 0.026	5.478GlyAsp: 5.478 ± 0.075	4.662GlyGlu: 4.662 ± 0.063	3.01GlyPhe: 3.01 ± 0.054	8.668GlyGly: 8.668 ± 0.12	2.239GlyHis: 2.239 ± 0.044	3.682GlyIle: 3.682 ± 0.063	2.358GlyLys: 2.358 ± 0.054	9.617GlyLeu: 9.617 ± 0.096	2.031GlyMet: 2.031 ± 0.041	1.741GlyAsn: 1.741 ± 0.04	4.624GlyPro: 4.624 ± 0.068	2.89GlyGln: 2.89 ± 0.055	7.097GlyArg: 7.097 ± 0.097	5.978GlySer: 5.978 ± 0.09	6.342GlyThr: 6.342 ± 0.086	8.189GlyVal: 8.189 ± 0.091	1.774GlyTrp: 1.774 ± 0.036	2.174GlyTyr: 2.174 ± 0.041	0.002GlyXaa: 0.002 ± 0.001
His	2.523HisAla: 2.523 ± 0.055	0.184HisCys: 0.184 ± 0.013	1.53HisAsp: 1.53 ± 0.042	1.341HisGlu: 1.341 ± 0.035	0.564HisPhe: 0.564 ± 0.022	2.262HisGly: 2.262 ± 0.052	0.729HisHis: 0.729 ± 0.027	0.564HisIle: 0.564 ± 0.024	0.305HisLys: 0.305 ± 0.016	2.503HisLeu: 2.503 ± 0.052	0.308HisMet: 0.308 ± 0.015	0.344HisAsn: 0.344 ± 0.019	1.601HisPro: 1.601 ± 0.038	0.616HisGln: 0.616 ± 0.023	1.76HisArg: 1.76 ± 0.041	0.963HisSer: 0.963 ± 0.031	1.183HisThr: 1.183 ± 0.032	2.074HisVal: 2.074 ± 0.043	0.375HisTrp: 0.375 ± 0.017	0.426HisTyr: 0.426 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	4.784IleAla: 4.784 ± 0.068	0.26IleCys: 0.26 ± 0.015	2.37IleAsp: 2.37 ± 0.046	2.017IleGlu: 2.017 ± 0.045	0.786IlePhe: 0.786 ± 0.025	3.616IleGly: 3.616 ± 0.07	0.641IleHis: 0.641 ± 0.023	1.022IleIle: 1.022 ± 0.035	0.75IleLys: 0.75 ± 0.032	2.474IleLeu: 2.474 ± 0.047	0.441IleMet: 0.441 ± 0.02	0.711IleAsn: 0.711 ± 0.026	1.791IlePro: 1.791 ± 0.039	0.751IleGln: 0.751 ± 0.028	2.144IleArg: 2.144 ± 0.044	1.831IleSer: 1.831 ± 0.047	2.127IleThr: 2.127 ± 0.045	3.118IleVal: 3.118 ± 0.058	0.388IleTrp: 0.388 ± 0.017	0.565IleTyr: 0.565 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	3.058LysAla: 3.058 ± 0.072	0.093LysCys: 0.093 ± 0.009	1.445LysAsp: 1.445 ± 0.037	1.036LysGlu: 1.036 ± 0.035	0.514LysPhe: 0.514 ± 0.02	1.918LysGly: 1.918 ± 0.052	0.396LysHis: 0.396 ± 0.015	0.845LysIle: 0.845 ± 0.028	0.753LysLys: 0.753 ± 0.039	1.688LysLeu: 1.688 ± 0.04	0.384LysMet: 0.384 ± 0.02	0.512LysAsn: 0.512 ± 0.025	1.244LysPro: 1.244 ± 0.041	0.658LysGln: 0.658 ± 0.026	1.288LysArg: 1.288 ± 0.039	1.204LysSer: 1.204 ± 0.035	1.302LysThr: 1.302 ± 0.039	2.231LysVal: 2.231 ± 0.055	0.227LysTrp: 0.227 ± 0.013	0.441LysTyr: 0.441 ± 0.019	0.001LysXaa: 0.001 ± 0.001
Leu	14.738LeuAla: 14.738 ± 0.133	0.678LeuCys: 0.678 ± 0.022	6.66LeuAsp: 6.66 ± 0.088	5.157LeuGlu: 5.157 ± 0.087	2.447LeuPhe: 2.447 ± 0.049	9.929LeuGly: 9.929 ± 0.115	2.021LeuHis: 2.021 ± 0.047	2.715LeuIle: 2.715 ± 0.057	1.884LeuLys: 1.884 ± 0.045	10.463LeuLeu: 10.463 ± 0.138	1.619LeuMet: 1.619 ± 0.037	1.572LeuAsn: 1.572 ± 0.035	5.762LeuPro: 5.762 ± 0.079	2.473LeuGln: 2.473 ± 0.05	7.83LeuArg: 7.83 ± 0.107	5.544LeuSer: 5.544 ± 0.072	6.446LeuThr: 6.446 ± 0.08	10.764LeuVal: 10.764 ± 0.128	1.257LeuTrp: 1.257 ± 0.037	1.503LeuTyr: 1.503 ± 0.039	0.002LeuXaa: 0.002 ± 0.001
Met	2.456MetAla: 2.456 ± 0.045	0.123MetCys: 0.123 ± 0.011	0.926MetAsp: 0.926 ± 0.032	0.737MetGlu: 0.737 ± 0.024	0.488MetPhe: 0.488 ± 0.02	1.43MetGly: 1.43 ± 0.034	0.325MetHis: 0.325 ± 0.017	0.589MetIle: 0.589 ± 0.024	0.481MetLys: 0.481 ± 0.018	1.795MetLeu: 1.795 ± 0.038	0.308MetMet: 0.308 ± 0.021	0.391MetAsn: 0.391 ± 0.018	1.069MetPro: 1.069 ± 0.031	0.466MetGln: 0.466 ± 0.022	1.372MetArg: 1.372 ± 0.033	1.461MetSer: 1.461 ± 0.036	1.651MetThr: 1.651 ± 0.036	1.6MetVal: 1.6 ± 0.036	0.195MetTrp: 0.195 ± 0.011	0.284MetTyr: 0.284 ± 0.016	0.001MetXaa: 0.001 ± 0.001
Asn	2.228AsnAla: 2.228 ± 0.046	0.143AsnCys: 0.143 ± 0.01	1.047AsnAsp: 1.047 ± 0.033	0.785AsnGlu: 0.785 ± 0.027	0.507AsnPhe: 0.507 ± 0.023	1.813AsnGly: 1.813 ± 0.054	0.396AsnHis: 0.396 ± 0.018	0.707AsnIle: 0.707 ± 0.027	0.34AsnLys: 0.34 ± 0.019	1.9AsnLeu: 1.9 ± 0.042	0.26AsnMet: 0.26 ± 0.015	0.443AsnAsn: 0.443 ± 0.023	1.446AsnPro: 1.446 ± 0.039	0.508AsnGln: 0.508 ± 0.023	1.183AsnArg: 1.183 ± 0.03	0.908AsnSer: 0.908 ± 0.032	1.074AsnThr: 1.074 ± 0.037	1.489AsnVal: 1.489 ± 0.037	0.261AsnTrp: 0.261 ± 0.016	0.389AsnTyr: 0.389 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	7.312ProAla: 7.312 ± 0.098	0.278ProCys: 0.278 ± 0.016	4.157ProAsp: 4.157 ± 0.07	3.496ProGlu: 3.496 ± 0.058	1.493ProPhe: 1.493 ± 0.036	5.8ProGly: 5.8 ± 0.07	1.235ProHis: 1.235 ± 0.035	1.5ProIle: 1.5 ± 0.04	1.129ProLys: 1.129 ± 0.033	4.72ProLeu: 4.72 ± 0.073	1.031ProMet: 1.031 ± 0.031	0.903ProAsn: 0.903 ± 0.024	2.69ProPro: 2.69 ± 0.064	1.652ProGln: 1.652 ± 0.043	3.587ProArg: 3.587 ± 0.053	3.422ProSer: 3.422 ± 0.063	3.844ProThr: 3.844 ± 0.067	5.475ProVal: 5.475 ± 0.072	0.921ProTrp: 0.921 ± 0.031	1.048ProTyr: 1.048 ± 0.03	0.002ProXaa: 0.002 ± 0.001
Gln	3.671GlnAla: 3.671 ± 0.058	0.165GlnCys: 0.165 ± 0.014	1.479GlnAsp: 1.479 ± 0.037	1.353GlnGlu: 1.353 ± 0.036	0.82GlnPhe: 0.82 ± 0.028	2.425GlnGly: 2.425 ± 0.049	0.672GlnHis: 0.672 ± 0.023	1.023GlnIle: 1.023 ± 0.032	0.61GlnLys: 0.61 ± 0.023	3.051GlnLeu: 3.051 ± 0.059	0.544GlnMet: 0.544 ± 0.023	0.514GlnAsn: 0.514 ± 0.022	1.637GlnPro: 1.637 ± 0.043	1.233GlnGln: 1.233 ± 0.041	2.517GlnArg: 2.517 ± 0.054	1.417GlnSer: 1.417 ± 0.031	1.534GlnThr: 1.534 ± 0.038	2.981GlnVal: 2.981 ± 0.05	0.53GlnTrp: 0.53 ± 0.022	0.483GlnTyr: 0.483 ± 0.019	0.001GlnXaa: 0.001 ± 0.001
Arg	9.038ArgAla: 9.038 ± 0.109	0.416ArgCys: 0.416 ± 0.017	4.426ArgAsp: 4.426 ± 0.073	4.305ArgGlu: 4.305 ± 0.069	2.121ArgPhe: 2.121 ± 0.043	5.853ArgGly: 5.853 ± 0.08	1.839ArgHis: 1.839 ± 0.049	2.682ArgIle: 2.682 ± 0.05	1.394ArgLys: 1.394 ± 0.041	7.706ArgLeu: 7.706 ± 0.095	1.635ArgMet: 1.635 ± 0.036	1.233ArgAsn: 1.233 ± 0.026	4.137ArgPro: 4.137 ± 0.06	2.129ArgGln: 2.129 ± 0.044	6.777ArgArg: 6.777 ± 0.093	4.373ArgSer: 4.373 ± 0.055	4.702ArgThr: 4.702 ± 0.057	6.234ArgVal: 6.234 ± 0.074	1.423ArgTrp: 1.423 ± 0.04	1.535ArgTyr: 1.535 ± 0.036	0.001ArgXaa: 0.001 ± 0.001
Ser	7.1SerAla: 7.1 ± 0.081	0.363SerCys: 0.363 ± 0.022	3.036SerAsp: 3.036 ± 0.046	2.515SerGlu: 2.515 ± 0.05	1.632SerPhe: 1.632 ± 0.039	6.126SerGly: 6.126 ± 0.081	1.103SerHis: 1.103 ± 0.031	1.883SerIle: 1.883 ± 0.042	1.119SerLys: 1.119 ± 0.036	5.424SerLeu: 5.424 ± 0.076	1.295SerMet: 1.295 ± 0.028	1.04SerAsn: 1.04 ± 0.03	3.304SerPro: 3.304 ± 0.058	1.525SerGln: 1.525 ± 0.041	4.067SerArg: 4.067 ± 0.065	3.742SerSer: 3.742 ± 0.078	4.049SerThr: 4.049 ± 0.058	4.892SerVal: 4.892 ± 0.064	1.01SerTrp: 1.01 ± 0.031	1.135SerTyr: 1.135 ± 0.034	0.002SerXaa: 0.002 ± 0.001
Thr	7.991ThrAla: 7.991 ± 0.108	0.446ThrCys: 0.446 ± 0.022	3.732ThrAsp: 3.732 ± 0.064	2.857ThrGlu: 2.857 ± 0.053	1.827ThrPhe: 1.827 ± 0.04	6.422ThrGly: 6.422 ± 0.079	1.226ThrHis: 1.226 ± 0.032	2.261ThrIle: 2.261 ± 0.056	1.415ThrLys: 1.415 ± 0.041	5.936ThrLeu: 5.936 ± 0.071	1.164ThrMet: 1.164 ± 0.041	1.227ThrAsn: 1.227 ± 0.036	4.137ThrPro: 4.137 ± 0.066	1.563ThrGln: 1.563 ± 0.039	4.127ThrArg: 4.127 ± 0.055	4.068ThrSer: 4.068 ± 0.066	5.03ThrThr: 5.03 ± 0.1	6.48ThrVal: 6.48 ± 0.085	1.073ThrTrp: 1.073 ± 0.032	1.345ThrTyr: 1.345 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	13.059ValAla: 13.059 ± 0.124	0.667ValCys: 0.667 ± 0.023	6.27ValAsp: 6.27 ± 0.075	5.292ValGlu: 5.292 ± 0.073	2.542ValPhe: 2.542 ± 0.049	8.225ValGly: 8.225 ± 0.092	2.016ValHis: 2.016 ± 0.043	3.19ValIle: 3.19 ± 0.052	1.875ValLys: 1.875 ± 0.045	10.438ValLeu: 10.438 ± 0.132	1.628ValMet: 1.628 ± 0.038	1.788ValAsn: 1.788 ± 0.043	5.222ValPro: 5.222 ± 0.068	2.444ValGln: 2.444 ± 0.052	6.798ValArg: 6.798 ± 0.092	5.063ValSer: 5.063 ± 0.073	6.451ValThr: 6.451 ± 0.072	11.328ValVal: 11.328 ± 0.14	1.218ValTrp: 1.218 ± 0.036	1.432ValTyr: 1.432 ± 0.04	0.001ValXaa: 0.001 ± 0.001
Trp	1.757TrpAla: 1.757 ± 0.039	0.133TrpCys: 0.133 ± 0.011	0.846TrpAsp: 0.846 ± 0.027	0.637TrpGlu: 0.637 ± 0.026	0.554TrpPhe: 0.554 ± 0.021	1.144TrpGly: 1.144 ± 0.035	0.381TrpHis: 0.381 ± 0.019	0.483TrpIle: 0.483 ± 0.02	0.309TrpLys: 0.309 ± 0.017	1.773TrpLeu: 1.773 ± 0.047	0.303TrpMet: 0.303 ± 0.014	0.363TrpAsn: 0.363 ± 0.018	0.795TrpPro: 0.795 ± 0.024	0.524TrpGln: 0.524 ± 0.019	1.221TrpArg: 1.221 ± 0.035	1.09TrpSer: 1.09 ± 0.03	1.029TrpThr: 1.029 ± 0.032	1.405TrpVal: 1.405 ± 0.032	0.409TrpTrp: 0.409 ± 0.019	0.278TrpTyr: 0.278 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.703TyrAla: 2.703 ± 0.048	0.125TyrCys: 0.125 ± 0.011	1.351TyrAsp: 1.351 ± 0.033	0.937TyrGlu: 0.937 ± 0.03	0.577TyrPhe: 0.577 ± 0.022	1.924TyrGly: 1.924 ± 0.046	0.319TyrHis: 0.319 ± 0.017	0.464TyrIle: 0.464 ± 0.019	0.356TyrLys: 0.356 ± 0.019	2.011TyrLeu: 2.011 ± 0.04	0.192TyrMet: 0.192 ± 0.013	0.369TyrAsn: 0.369 ± 0.022	0.975TyrPro: 0.975 ± 0.03	0.533TyrGln: 0.533 ± 0.022	1.451TyrArg: 1.451 ± 0.04	0.975TyrSer: 0.975 ± 0.031	1.08TyrThr: 1.08 ± 0.032	1.642TyrVal: 1.642 ± 0.041	0.282TyrTrp: 0.282 ± 0.017	0.406TyrTyr: 0.406 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.003

Statistics based on 3714 proteins (1203151 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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