Amino acid dipepetide frequency for Pantoea phage vB_PagS_AAS23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.124AlaAla: 10.124 ± 1.668
0.812AlaCys: 0.812 ± 0.272
4.812AlaAsp: 4.812 ± 0.532
6.125AlaGlu: 6.125 ± 0.699
2.625AlaPhe: 2.625 ± 0.486
7.5AlaGly: 7.5 ± 0.728
1.062AlaHis: 1.062 ± 0.256
4.812AlaIle: 4.812 ± 0.711
6.5AlaLys: 6.5 ± 0.839
6.5AlaLeu: 6.5 ± 0.712
3.375AlaMet: 3.375 ± 0.428
3.625AlaAsn: 3.625 ± 0.561
2.062AlaPro: 2.062 ± 0.296
4.25AlaGln: 4.25 ± 0.713
4.875AlaArg: 4.875 ± 0.493
7.25AlaSer: 7.25 ± 0.893
4.375AlaThr: 4.375 ± 0.625
6.375AlaVal: 6.375 ± 0.711
1.5AlaTrp: 1.5 ± 0.256
2.0AlaTyr: 2.0 ± 0.308
0.0AlaXaa: 0.0 ± 0.0
Cys
1.0CysAla: 1.0 ± 0.248
0.312CysCys: 0.312 ± 0.161
1.125CysAsp: 1.125 ± 0.311
0.937CysGlu: 0.937 ± 0.309
0.75CysPhe: 0.75 ± 0.242
1.125CysGly: 1.125 ± 0.334
0.375CysHis: 0.375 ± 0.155
1.125CysIle: 1.125 ± 0.248
0.875CysLys: 0.875 ± 0.252
1.125CysLeu: 1.125 ± 0.238
0.437CysMet: 0.437 ± 0.142
0.562CysAsn: 0.562 ± 0.202
0.75CysPro: 0.75 ± 0.212
0.062CysGln: 0.062 ± 0.059
1.0CysArg: 1.0 ± 0.271
0.875CysSer: 0.875 ± 0.274
0.625CysThr: 0.625 ± 0.219
1.125CysVal: 1.125 ± 0.335
0.375CysTrp: 0.375 ± 0.157
0.312CysTyr: 0.312 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
5.437AspAla: 5.437 ± 0.544
0.75AspCys: 0.75 ± 0.275
4.437AspAsp: 4.437 ± 0.537
4.187AspGlu: 4.187 ± 0.541
2.187AspPhe: 2.187 ± 0.45
5.437AspGly: 5.437 ± 0.625
0.687AspHis: 0.687 ± 0.199
4.187AspIle: 4.187 ± 0.421
3.875AspLys: 3.875 ± 0.505
4.937AspLeu: 4.937 ± 0.517
1.812AspMet: 1.812 ± 0.317
1.937AspAsn: 1.937 ± 0.438
1.562AspPro: 1.562 ± 0.234
2.187AspGln: 2.187 ± 0.366
2.437AspArg: 2.437 ± 0.416
4.562AspSer: 4.562 ± 0.573
2.25AspThr: 2.25 ± 0.285
3.625AspVal: 3.625 ± 0.358
1.125AspTrp: 1.125 ± 0.28
2.562AspTyr: 2.562 ± 0.425
0.0AspXaa: 0.0 ± 0.0
Glu
5.25GluAla: 5.25 ± 0.534
0.875GluCys: 0.875 ± 0.231
2.937GluAsp: 2.937 ± 0.42
4.062GluGlu: 4.062 ± 0.571
3.562GluPhe: 3.562 ± 0.493
2.937GluGly: 2.937 ± 0.384
1.25GluHis: 1.25 ± 0.239
4.75GluIle: 4.75 ± 0.526
3.5GluLys: 3.5 ± 0.428
4.187GluLeu: 4.187 ± 0.521
2.687GluMet: 2.687 ± 0.445
2.562GluAsn: 2.562 ± 0.523
2.375GluPro: 2.375 ± 0.41
2.812GluGln: 2.812 ± 0.546
3.187GluArg: 3.187 ± 0.37
4.875GluSer: 4.875 ± 0.411
3.187GluThr: 3.187 ± 0.469
4.625GluVal: 4.625 ± 0.513
1.062GluTrp: 1.062 ± 0.287
2.312GluTyr: 2.312 ± 0.334
0.0GluXaa: 0.0 ± 0.0
Phe
3.812PheAla: 3.812 ± 0.515
0.562PheCys: 0.562 ± 0.214
3.062PheAsp: 3.062 ± 0.504
2.687PheGlu: 2.687 ± 0.389
1.375PhePhe: 1.375 ± 0.319
3.875PheGly: 3.875 ± 0.507
0.562PheHis: 0.562 ± 0.192
2.375PheIle: 2.375 ± 0.421
2.687PheLys: 2.687 ± 0.426
2.062PheLeu: 2.062 ± 0.389
1.125PheMet: 1.125 ± 0.293
2.375PheAsn: 2.375 ± 0.436
1.187PhePro: 1.187 ± 0.318
1.187PheGln: 1.187 ± 0.272
2.187PheArg: 2.187 ± 0.431
2.625PheSer: 2.625 ± 0.44
2.75PheThr: 2.75 ± 0.462
2.437PheVal: 2.437 ± 0.407
0.687PheTrp: 0.687 ± 0.201
1.25PheTyr: 1.25 ± 0.299
0.0PheXaa: 0.0 ± 0.0
Gly
5.125GlyAla: 5.125 ± 0.682
1.125GlyCys: 1.125 ± 0.262
4.25GlyAsp: 4.25 ± 0.549
4.312GlyGlu: 4.312 ± 0.458
3.625GlyPhe: 3.625 ± 0.511
7.062GlyGly: 7.062 ± 1.0
1.562GlyHis: 1.562 ± 0.422
3.625GlyIle: 3.625 ± 0.584
5.187GlyLys: 5.187 ± 0.491
5.437GlyLeu: 5.437 ± 0.52
2.0GlyMet: 2.0 ± 0.386
3.687GlyAsn: 3.687 ± 0.529
1.25GlyPro: 1.25 ± 0.302
2.875GlyGln: 2.875 ± 0.433
3.687GlyArg: 3.687 ± 0.544
4.187GlySer: 4.187 ± 0.569
3.875GlyThr: 3.875 ± 0.507
5.0GlyVal: 5.0 ± 0.673
1.437GlyTrp: 1.437 ± 0.312
3.375GlyTyr: 3.375 ± 0.487
0.0GlyXaa: 0.0 ± 0.0
His
1.187HisAla: 1.187 ± 0.291
0.312HisCys: 0.312 ± 0.119
1.375HisAsp: 1.375 ± 0.333
1.25HisGlu: 1.25 ± 0.27
0.625HisPhe: 0.625 ± 0.209
1.25HisGly: 1.25 ± 0.337
0.312HisHis: 0.312 ± 0.153
1.187HisIle: 1.187 ± 0.324
0.937HisLys: 0.937 ± 0.213
1.25HisLeu: 1.25 ± 0.291
0.562HisMet: 0.562 ± 0.238
0.437HisAsn: 0.437 ± 0.211
0.875HisPro: 0.875 ± 0.282
0.875HisGln: 0.875 ± 0.253
1.687HisArg: 1.687 ± 0.319
1.187HisSer: 1.187 ± 0.268
0.625HisThr: 0.625 ± 0.206
1.437HisVal: 1.437 ± 0.342
0.25HisTrp: 0.25 ± 0.127
0.937HisTyr: 0.937 ± 0.206
0.0HisXaa: 0.0 ± 0.0
Ile
6.062IleAla: 6.062 ± 0.587
0.875IleCys: 0.875 ± 0.205
4.25IleAsp: 4.25 ± 0.495
4.375IleGlu: 4.375 ± 0.533
2.75IlePhe: 2.75 ± 0.426
4.25IleGly: 4.25 ± 0.522
1.062IleHis: 1.062 ± 0.251
3.687IleIle: 3.687 ± 0.375
4.75IleLys: 4.75 ± 0.617
3.75IleLeu: 3.75 ± 0.478
1.812IleMet: 1.812 ± 0.318
3.0IleAsn: 3.0 ± 0.491
2.187IlePro: 2.187 ± 0.33
2.187IleGln: 2.187 ± 0.362
2.937IleArg: 2.937 ± 0.392
4.0IleSer: 4.0 ± 0.416
4.0IleThr: 4.0 ± 0.562
3.875IleVal: 3.875 ± 0.427
0.75IleTrp: 0.75 ± 0.235
2.0IleTyr: 2.0 ± 0.33
0.0IleXaa: 0.0 ± 0.0
Lys
6.25LysAla: 6.25 ± 0.937
1.125LysCys: 1.125 ± 0.317
4.875LysAsp: 4.875 ± 0.592
3.562LysGlu: 3.562 ± 0.484
2.062LysPhe: 2.062 ± 0.369
3.562LysGly: 3.562 ± 0.4
1.375LysHis: 1.375 ± 0.292
4.187LysIle: 4.187 ± 0.468
4.0LysLys: 4.0 ± 0.672
5.437LysLeu: 5.437 ± 0.631
2.437LysMet: 2.437 ± 0.409
2.812LysAsn: 2.812 ± 0.478
3.0LysPro: 3.0 ± 0.416
3.125LysGln: 3.125 ± 0.661
2.875LysArg: 2.875 ± 0.462
3.562LysSer: 3.562 ± 0.607
3.625LysThr: 3.625 ± 0.494
4.375LysVal: 4.375 ± 0.445
0.75LysTrp: 0.75 ± 0.241
1.5LysTyr: 1.5 ± 0.278
0.0LysXaa: 0.0 ± 0.0
Leu
7.437LeuAla: 7.437 ± 0.725
0.937LeuCys: 0.937 ± 0.277
3.125LeuAsp: 3.125 ± 0.44
3.687LeuGlu: 3.687 ± 0.492
2.437LeuPhe: 2.437 ± 0.472
4.187LeuGly: 4.187 ± 0.474
1.5LeuHis: 1.5 ± 0.355
4.312LeuIle: 4.312 ± 0.448
5.125LeuLys: 5.125 ± 0.455
4.187LeuLeu: 4.187 ± 0.587
1.937LeuMet: 1.937 ± 0.333
3.25LeuAsn: 3.25 ± 0.4
2.875LeuPro: 2.875 ± 0.384
2.562LeuGln: 2.562 ± 0.452
4.375LeuArg: 4.375 ± 0.618
5.187LeuSer: 5.187 ± 0.59
4.187LeuThr: 4.187 ± 0.41
4.562LeuVal: 4.562 ± 0.468
0.687LeuTrp: 0.687 ± 0.242
2.75LeuTyr: 2.75 ± 0.409
0.0LeuXaa: 0.0 ± 0.0
Met
2.75MetAla: 2.75 ± 0.34
0.312MetCys: 0.312 ± 0.147
1.25MetAsp: 1.25 ± 0.294
0.875MetGlu: 0.875 ± 0.242
1.0MetPhe: 1.0 ± 0.225
1.562MetGly: 1.562 ± 0.41
0.75MetHis: 0.75 ± 0.214
1.937MetIle: 1.937 ± 0.298
3.062MetLys: 3.062 ± 0.443
2.25MetLeu: 2.25 ± 0.417
0.937MetMet: 0.937 ± 0.21
1.937MetAsn: 1.937 ± 0.378
1.25MetPro: 1.25 ± 0.279
0.875MetGln: 0.875 ± 0.246
2.187MetArg: 2.187 ± 0.366
2.25MetSer: 2.25 ± 0.385
2.187MetThr: 2.187 ± 0.382
1.75MetVal: 1.75 ± 0.257
0.375MetTrp: 0.375 ± 0.132
0.812MetTyr: 0.812 ± 0.238
0.0MetXaa: 0.0 ± 0.0
Asn
4.875AsnAla: 4.875 ± 0.705
0.687AsnCys: 0.687 ± 0.23
2.437AsnAsp: 2.437 ± 0.372
2.937AsnGlu: 2.937 ± 0.462
1.125AsnPhe: 1.125 ± 0.267
4.437AsnGly: 4.437 ± 0.621
0.875AsnHis: 0.875 ± 0.236
2.375AsnIle: 2.375 ± 0.451
2.25AsnLys: 2.25 ± 0.375
3.187AsnLeu: 3.187 ± 0.461
1.125AsnMet: 1.125 ± 0.232
1.937AsnAsn: 1.937 ± 0.416
2.75AsnPro: 2.75 ± 0.459
1.937AsnGln: 1.937 ± 0.445
1.937AsnArg: 1.937 ± 0.337
3.937AsnSer: 3.937 ± 0.443
2.125AsnThr: 2.125 ± 0.309
2.562AsnVal: 2.562 ± 0.416
0.5AsnTrp: 0.5 ± 0.132
1.562AsnTyr: 1.562 ± 0.383
0.0AsnXaa: 0.0 ± 0.0
Pro
2.937ProAla: 2.937 ± 0.471
0.562ProCys: 0.562 ± 0.204
2.937ProAsp: 2.937 ± 0.443
3.125ProGlu: 3.125 ± 0.534
1.687ProPhe: 1.687 ± 0.271
2.875ProGly: 2.875 ± 0.443
0.687ProHis: 0.687 ± 0.221
1.937ProIle: 1.937 ± 0.332
1.187ProLys: 1.187 ± 0.256
2.625ProLeu: 2.625 ± 0.332
0.937ProMet: 0.937 ± 0.202
1.625ProAsn: 1.625 ± 0.274
1.062ProPro: 1.062 ± 0.232
1.812ProGln: 1.812 ± 0.36
1.687ProArg: 1.687 ± 0.338
1.687ProSer: 1.687 ± 0.324
1.375ProThr: 1.375 ± 0.367
2.75ProVal: 2.75 ± 0.382
0.5ProTrp: 0.5 ± 0.195
1.25ProTyr: 1.25 ± 0.26
0.0ProXaa: 0.0 ± 0.0
Gln
4.062GlnAla: 4.062 ± 0.779
0.25GlnCys: 0.25 ± 0.128
2.375GlnAsp: 2.375 ± 0.314
2.5GlnGlu: 2.5 ± 0.478
1.312GlnPhe: 1.312 ± 0.357
1.937GlnGly: 1.937 ± 0.377
0.937GlnHis: 0.937 ± 0.272
3.0GlnIle: 3.0 ± 0.52
2.687GlnLys: 2.687 ± 0.361
2.312GlnLeu: 2.312 ± 0.443
1.25GlnMet: 1.25 ± 0.299
1.937GlnAsn: 1.937 ± 0.329
2.125GlnPro: 2.125 ± 0.377
3.062GlnGln: 3.062 ± 0.461
1.875GlnArg: 1.875 ± 0.35
2.75GlnSer: 2.75 ± 0.485
2.187GlnThr: 2.187 ± 0.508
3.437GlnVal: 3.437 ± 0.476
0.5GlnTrp: 0.5 ± 0.181
1.375GlnTyr: 1.375 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
4.187ArgAla: 4.187 ± 0.442
1.187ArgCys: 1.187 ± 0.306
2.937ArgAsp: 2.937 ± 0.502
3.562ArgGlu: 3.562 ± 0.442
3.062ArgPhe: 3.062 ± 0.466
2.687ArgGly: 2.687 ± 0.397
1.125ArgHis: 1.125 ± 0.314
3.812ArgIle: 3.812 ± 0.463
3.625ArgLys: 3.625 ± 0.468
3.937ArgLeu: 3.937 ± 0.646
1.75ArgMet: 1.75 ± 0.324
2.187ArgAsn: 2.187 ± 0.435
1.687ArgPro: 1.687 ± 0.375
2.062ArgGln: 2.062 ± 0.424
3.0ArgArg: 3.0 ± 0.419
3.437ArgSer: 3.437 ± 0.498
1.875ArgThr: 1.875 ± 0.276
4.0ArgVal: 4.0 ± 0.613
0.562ArgTrp: 0.562 ± 0.181
2.125ArgTyr: 2.125 ± 0.398
0.0ArgXaa: 0.0 ± 0.0
Ser
6.875SerAla: 6.875 ± 1.048
1.0SerCys: 1.0 ± 0.262
4.187SerAsp: 4.187 ± 0.604
4.187SerGlu: 4.187 ± 0.506
2.875SerPhe: 2.875 ± 0.388
6.375SerGly: 6.375 ± 0.743
1.5SerHis: 1.5 ± 0.367
4.25SerIle: 4.25 ± 0.53
4.437SerLys: 4.437 ± 0.466
4.75SerLeu: 4.75 ± 0.573
1.812SerMet: 1.812 ± 0.312
2.562SerAsn: 2.562 ± 0.422
1.937SerPro: 1.937 ± 0.387
2.5SerGln: 2.5 ± 0.445
3.187SerArg: 3.187 ± 0.468
4.0SerSer: 4.0 ± 0.635
3.375SerThr: 3.375 ± 0.403
5.375SerVal: 5.375 ± 0.633
0.75SerTrp: 0.75 ± 0.22
2.375SerTyr: 2.375 ± 0.445
0.0SerXaa: 0.0 ± 0.0
Thr
4.687ThrAla: 4.687 ± 0.63
0.75ThrCys: 0.75 ± 0.228
2.687ThrAsp: 2.687 ± 0.502
3.375ThrGlu: 3.375 ± 0.458
3.125ThrPhe: 3.125 ± 0.464
3.937ThrGly: 3.937 ± 0.349
0.937ThrHis: 0.937 ± 0.23
3.125ThrIle: 3.125 ± 0.424
3.25ThrLys: 3.25 ± 0.493
3.375ThrLeu: 3.375 ± 0.406
1.125ThrMet: 1.125 ± 0.295
3.312ThrAsn: 3.312 ± 0.496
2.5ThrPro: 2.5 ± 0.45
1.812ThrGln: 1.812 ± 0.47
2.25ThrArg: 2.25 ± 0.313
3.25ThrSer: 3.25 ± 0.498
2.5ThrThr: 2.5 ± 0.348
3.625ThrVal: 3.625 ± 0.437
0.625ThrTrp: 0.625 ± 0.226
1.562ThrTyr: 1.562 ± 0.296
0.0ThrXaa: 0.0 ± 0.0
Val
5.625ValAla: 5.625 ± 0.693
1.312ValCys: 1.312 ± 0.293
3.937ValAsp: 3.937 ± 0.383
4.625ValGlu: 4.625 ± 0.527
2.562ValPhe: 2.562 ± 0.423
4.437ValGly: 4.437 ± 0.695
1.187ValHis: 1.187 ± 0.278
4.437ValIle: 4.437 ± 0.557
4.625ValLys: 4.625 ± 0.617
4.437ValLeu: 4.437 ± 0.605
2.062ValMet: 2.062 ± 0.311
3.5ValAsn: 3.5 ± 0.608
2.062ValPro: 2.062 ± 0.416
2.875ValGln: 2.875 ± 0.606
3.625ValArg: 3.625 ± 0.461
4.937ValSer: 4.937 ± 0.484
3.937ValThr: 3.937 ± 0.442
5.437ValVal: 5.437 ± 0.634
0.687ValTrp: 0.687 ± 0.168
2.75ValTyr: 2.75 ± 0.433
0.0ValXaa: 0.0 ± 0.0
Trp
1.0TrpAla: 1.0 ± 0.285
0.437TrpCys: 0.437 ± 0.154
0.875TrpAsp: 0.875 ± 0.269
0.562TrpGlu: 0.562 ± 0.229
0.625TrpPhe: 0.625 ± 0.197
1.062TrpGly: 1.062 ± 0.2
0.312TrpHis: 0.312 ± 0.128
1.25TrpIle: 1.25 ± 0.225
0.75TrpLys: 0.75 ± 0.177
0.937TrpLeu: 0.937 ± 0.248
0.312TrpMet: 0.312 ± 0.169
0.75TrpAsn: 0.75 ± 0.219
0.312TrpPro: 0.312 ± 0.169
0.875TrpGln: 0.875 ± 0.26
0.875TrpArg: 0.875 ± 0.222
0.75TrpSer: 0.75 ± 0.264
0.625TrpThr: 0.625 ± 0.185
1.0TrpVal: 1.0 ± 0.235
0.187TrpTrp: 0.187 ± 0.113
0.312TrpTyr: 0.312 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.875TyrAla: 1.875 ± 0.351
0.687TyrCys: 0.687 ± 0.216
2.312TyrAsp: 2.312 ± 0.406
2.062TyrGlu: 2.062 ± 0.349
1.5TyrPhe: 1.5 ± 0.328
2.187TyrGly: 2.187 ± 0.448
0.5TyrHis: 0.5 ± 0.173
2.25TyrIle: 2.25 ± 0.39
1.187TyrLys: 1.187 ± 0.257
2.625TyrLeu: 2.625 ± 0.38
0.75TyrMet: 0.75 ± 0.24
1.562TyrAsn: 1.562 ± 0.311
1.437TyrPro: 1.437 ± 0.313
1.937TyrGln: 1.937 ± 0.317
2.937TyrArg: 2.937 ± 0.399
3.0TyrSer: 3.0 ± 0.426
2.125TyrThr: 2.125 ± 0.285
1.687TyrVal: 1.687 ± 0.287
0.5TyrTrp: 0.5 ± 0.172
1.0TyrTyr: 1.0 ± 0.258
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (16002 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski