Phytophthora infestans (strain T30-4) (Potato late blight fungus)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Phytophthora; Phytophthora infestans

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17610 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0NM77|D0NM77_PHYIT Secreted RxLR effector peptide protein putative (Fragment) OS=Phytophthora infestans (strain T30-4) OX=403677 GN=PITG_13535 PE=4 SV=1
MM1 pKa = 7.55SPHH4 pKa = 6.67CLSLLVAFGVSFTAAVEE21 pKa = 4.14VSVCRR26 pKa = 11.84DD27 pKa = 2.99ATYY30 pKa = 10.46DD31 pKa = 3.28ISVDD35 pKa = 3.45ATSLCSGSGAAPAGWSCPKK54 pKa = 10.71AGDD57 pKa = 3.7VAVADD62 pKa = 4.52CLSTLASHH70 pKa = 7.36GSGTCVAPEE79 pKa = 3.94DD80 pKa = 4.25AVCQVVNGDD89 pKa = 2.84TWGCVLPSVGCNTPVVADD107 pKa = 4.16SACEE111 pKa = 3.64TWDD114 pKa = 3.42YY115 pKa = 11.83SGDD118 pKa = 3.77DD119 pKa = 3.88TVDD122 pKa = 3.09SSGSFDD128 pKa = 3.57GNEE131 pKa = 4.85DD132 pKa = 3.59YY133 pKa = 11.56DD134 pKa = 4.09EE135 pKa = 4.15TWFMKK140 pKa = 8.33TTEE143 pKa = 4.02LRR145 pKa = 11.84EE146 pKa = 4.62LYY148 pKa = 10.34DD149 pKa = 3.69CSKK152 pKa = 10.83KK153 pKa = 9.0PTPAPTTPCPTPAATEE169 pKa = 4.3TNTTEE174 pKa = 4.42APTATTAPAPTDD186 pKa = 3.52SSDD189 pKa = 3.49TDD191 pKa = 3.87PVTLAPTPTVTPIPMPTEE209 pKa = 4.28VNGSSSDD216 pKa = 3.6VMSDD220 pKa = 2.67GVAQNRR226 pKa = 11.84EE227 pKa = 4.17TEE229 pKa = 4.2VGDD232 pKa = 3.85EE233 pKa = 3.92EE234 pKa = 4.92SAAGTHH240 pKa = 5.14TVVAFAAADD249 pKa = 3.51AVSFGGLSNEE259 pKa = 4.1VVAVIAAVAAFVAVIVAAVAIVYY282 pKa = 9.76ASKK285 pKa = 10.55RR286 pKa = 11.84HH287 pKa = 5.27AKK289 pKa = 10.14EE290 pKa = 3.72DD291 pKa = 3.64VEE293 pKa = 4.47EE294 pKa = 4.98GGEE297 pKa = 3.97KK298 pKa = 10.57DD299 pKa = 3.38EE300 pKa = 5.54SSDD303 pKa = 3.44EE304 pKa = 4.34GEE306 pKa = 4.22EE307 pKa = 4.2KK308 pKa = 10.73EE309 pKa = 4.38GDD311 pKa = 3.63VGEE314 pKa = 4.59DD315 pKa = 3.54AEE317 pKa = 5.55DD318 pKa = 3.63EE319 pKa = 4.65AEE321 pKa = 4.22STTYY325 pKa = 10.16PVAPPTPAVVMGEE338 pKa = 4.06MATTPVAASAKK349 pKa = 9.98AKK351 pKa = 8.74ATSPVTTPAVTSSPEE366 pKa = 3.87ATVEE370 pKa = 4.08TSSDD374 pKa = 3.48HH375 pKa = 6.9VSEE378 pKa = 4.39STDD381 pKa = 2.98GDD383 pKa = 4.02KK384 pKa = 10.77ATEE387 pKa = 4.14TSDD390 pKa = 3.52TPSSQEE396 pKa = 3.95TIAKK400 pKa = 10.17DD401 pKa = 3.46DD402 pKa = 3.56

Molecular weight: 40.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0N7X4|D0N7X4_PHYIT Uncharacterized protein OS=Phytophthora infestans (strain T30-4) OX=403677 GN=PITG_06714 PE=4 SV=1
LL1 pKa = 7.95LLVIWWWQRR10 pKa = 11.84LRR12 pKa = 11.84LLPRR16 pKa = 11.84LLFVVQWWVWQWKK29 pKa = 8.98WILPGLLLFLRR40 pKa = 11.84LPQLRR45 pKa = 11.84RR46 pKa = 11.84LRR48 pKa = 11.84LLQLRR53 pKa = 11.84VPGLLGLL60 pKa = 4.26

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17610

0

17610

7440462

29

10550

422.5

46.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.82 ± 0.018

1.677 ± 0.011

5.63 ± 0.014

6.476 ± 0.021

3.841 ± 0.011

6.12 ± 0.02

2.355 ± 0.008

4.181 ± 0.013

5.212 ± 0.02

9.534 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.009

3.576 ± 0.009

4.574 ± 0.017

4.182 ± 0.014

6.269 ± 0.02

8.124 ± 0.02

5.933 ± 0.019

7.113 ± 0.016

1.235 ± 0.007

2.674 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski