Amino acid dipepetide frequency for Microbacterium sp. BK668

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.199AlaAla: 21.199 ± 0.201
0.666AlaCys: 0.666 ± 0.023
8.503AlaAsp: 8.503 ± 0.093
8.253AlaGlu: 8.253 ± 0.111
4.027AlaPhe: 4.027 ± 0.064
12.582AlaGly: 12.582 ± 0.134
2.335AlaHis: 2.335 ± 0.052
5.865AlaIle: 5.865 ± 0.07
2.423AlaLys: 2.423 ± 0.054
13.983AlaLeu: 13.983 ± 0.146
2.512AlaMet: 2.512 ± 0.054
2.2AlaAsn: 2.2 ± 0.055
6.979AlaPro: 6.979 ± 0.107
3.732AlaGln: 3.732 ± 0.062
9.864AlaArg: 9.864 ± 0.143
7.971AlaSer: 7.971 ± 0.088
7.541AlaThr: 7.541 ± 0.118
12.01AlaVal: 12.01 ± 0.122
2.059AlaTrp: 2.059 ± 0.045
2.442AlaTyr: 2.442 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.67CysAla: 0.67 ± 0.024
0.042CysCys: 0.042 ± 0.006
0.311CysAsp: 0.311 ± 0.018
0.244CysGlu: 0.244 ± 0.014
0.165CysPhe: 0.165 ± 0.014
0.534CysGly: 0.534 ± 0.023
0.116CysHis: 0.116 ± 0.01
0.172CysIle: 0.172 ± 0.013
0.056CysLys: 0.056 ± 0.007
0.446CysLeu: 0.446 ± 0.022
0.066CysMet: 0.066 ± 0.01
0.104CysAsn: 0.104 ± 0.012
0.308CysPro: 0.308 ± 0.018
0.119CysGln: 0.119 ± 0.011
0.368CysArg: 0.368 ± 0.017
0.311CysSer: 0.311 ± 0.018
0.32CysThr: 0.32 ± 0.022
0.422CysVal: 0.422 ± 0.018
0.073CysTrp: 0.073 ± 0.009
0.096CysTyr: 0.096 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
9.568AspAla: 9.568 ± 0.116
0.258AspCys: 0.258 ± 0.017
4.58AspAsp: 4.58 ± 0.082
4.555AspGlu: 4.555 ± 0.076
1.791AspPhe: 1.791 ± 0.045
6.249AspGly: 6.249 ± 0.093
1.144AspHis: 1.144 ± 0.028
2.471AspIle: 2.471 ± 0.047
0.903AspLys: 0.903 ± 0.029
6.328AspLeu: 6.328 ± 0.087
0.821AspMet: 0.821 ± 0.03
0.923AspAsn: 0.923 ± 0.028
4.499AspPro: 4.499 ± 0.075
1.475AspGln: 1.475 ± 0.038
4.768AspArg: 4.768 ± 0.087
2.601AspSer: 2.601 ± 0.051
2.764AspThr: 2.764 ± 0.055
5.681AspVal: 5.681 ± 0.079
0.996AspTrp: 0.996 ± 0.032
1.284AspTyr: 1.284 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
7.488GluAla: 7.488 ± 0.115
0.244GluCys: 0.244 ± 0.015
2.808GluAsp: 2.808 ± 0.057
3.154GluGlu: 3.154 ± 0.067
1.707GluPhe: 1.707 ± 0.046
4.463GluGly: 4.463 ± 0.061
1.36GluHis: 1.36 ± 0.04
2.575GluIle: 2.575 ± 0.055
1.24GluLys: 1.24 ± 0.038
6.373GluLeu: 6.373 ± 0.096
0.751GluMet: 0.751 ± 0.029
1.129GluAsn: 1.129 ± 0.033
3.326GluPro: 3.326 ± 0.068
1.97GluGln: 1.97 ± 0.047
5.394GluArg: 5.394 ± 0.1
2.822GluSer: 2.822 ± 0.047
3.035GluThr: 3.035 ± 0.061
4.673GluVal: 4.673 ± 0.083
0.994GluTrp: 0.994 ± 0.033
1.131GluTyr: 1.131 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
4.432PheAla: 4.432 ± 0.074
0.151PheCys: 0.151 ± 0.013
2.417PheAsp: 2.417 ± 0.046
1.752PheGlu: 1.752 ± 0.039
1.102PhePhe: 1.102 ± 0.037
3.399PheGly: 3.399 ± 0.064
0.562PheHis: 0.562 ± 0.023
1.132PheIle: 1.132 ± 0.04
0.417PheLys: 0.417 ± 0.023
2.901PheLeu: 2.901 ± 0.056
0.401PheMet: 0.401 ± 0.018
0.637PheAsn: 0.637 ± 0.026
1.477PhePro: 1.477 ± 0.035
0.761PheGln: 0.761 ± 0.03
1.925PheArg: 1.925 ± 0.043
1.741PheSer: 1.741 ± 0.039
2.077PheThr: 2.077 ± 0.054
2.854PheVal: 2.854 ± 0.057
0.497PheTrp: 0.497 ± 0.02
0.668PheTyr: 0.668 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
11.18GlyAla: 11.18 ± 0.167
0.567GlyCys: 0.567 ± 0.022
5.537GlyAsp: 5.537 ± 0.079
5.231GlyGlu: 5.231 ± 0.073
3.333GlyPhe: 3.333 ± 0.053
8.189GlyGly: 8.189 ± 0.174
1.75GlyHis: 1.75 ± 0.04
4.773GlyIle: 4.773 ± 0.072
1.879GlyLys: 1.879 ± 0.045
8.577GlyLeu: 8.577 ± 0.096
1.919GlyMet: 1.919 ± 0.045
1.686GlyAsn: 1.686 ± 0.057
4.22GlyPro: 4.22 ± 0.071
2.396GlyGln: 2.396 ± 0.055
6.8GlyArg: 6.8 ± 0.092
5.619GlySer: 5.619 ± 0.106
5.54GlyThr: 5.54 ± 0.112
7.996GlyVal: 7.996 ± 0.097
1.755GlyTrp: 1.755 ± 0.039
2.279GlyTyr: 2.279 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.374HisAla: 2.374 ± 0.049
0.099HisCys: 0.099 ± 0.009
1.242HisAsp: 1.242 ± 0.037
1.109HisGlu: 1.109 ± 0.034
0.558HisPhe: 0.558 ± 0.022
1.919HisGly: 1.919 ± 0.045
0.52HisHis: 0.52 ± 0.024
0.718HisIle: 0.718 ± 0.027
0.25HisLys: 0.25 ± 0.014
1.917HisLeu: 1.917 ± 0.046
0.268HisMet: 0.268 ± 0.017
0.31HisAsn: 0.31 ± 0.017
1.53HisPro: 1.53 ± 0.046
0.416HisGln: 0.416 ± 0.019
1.564HisArg: 1.564 ± 0.039
0.907HisSer: 0.907 ± 0.028
0.905HisThr: 0.905 ± 0.029
1.679HisVal: 1.679 ± 0.038
0.287HisTrp: 0.287 ± 0.018
0.412HisTyr: 0.412 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.769IleAla: 6.769 ± 0.07
0.223IleCys: 0.223 ± 0.015
3.318IleAsp: 3.318 ± 0.056
2.695IleGlu: 2.695 ± 0.048
1.178IlePhe: 1.178 ± 0.036
4.52IleGly: 4.52 ± 0.072
0.674IleHis: 0.674 ± 0.026
1.603IleIle: 1.603 ± 0.048
0.693IleLys: 0.693 ± 0.028
3.815IleLeu: 3.815 ± 0.061
0.538IleMet: 0.538 ± 0.023
0.811IleAsn: 0.811 ± 0.033
2.558IlePro: 2.558 ± 0.049
0.989IleGln: 0.989 ± 0.034
2.711IleArg: 2.711 ± 0.052
2.198IleSer: 2.198 ± 0.045
2.494IleThr: 2.494 ± 0.052
4.651IleVal: 4.651 ± 0.074
0.502IleTrp: 0.502 ± 0.023
0.742IleTyr: 0.742 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
2.214LysAla: 2.214 ± 0.044
0.061LysCys: 0.061 ± 0.007
1.045LysAsp: 1.045 ± 0.038
0.86LysGlu: 0.86 ± 0.031
0.398LysPhe: 0.398 ± 0.018
1.521LysGly: 1.521 ± 0.042
0.383LysHis: 0.383 ± 0.019
0.776LysIle: 0.776 ± 0.026
0.587LysLys: 0.587 ± 0.029
1.601LysLeu: 1.601 ± 0.038
0.296LysMet: 0.296 ± 0.016
0.412LysAsn: 0.412 ± 0.018
1.11LysPro: 1.11 ± 0.037
0.559LysGln: 0.559 ± 0.025
1.405LysArg: 1.405 ± 0.039
0.988LysSer: 0.988 ± 0.032
1.196LysThr: 1.196 ± 0.037
1.469LysVal: 1.469 ± 0.04
0.218LysTrp: 0.218 ± 0.014
0.384LysTyr: 0.384 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
14.707LeuAla: 14.707 ± 0.15
0.498LeuCys: 0.498 ± 0.022
6.545LeuAsp: 6.545 ± 0.084
5.374LeuGlu: 5.374 ± 0.085
2.914LeuPhe: 2.914 ± 0.065
8.973LeuGly: 8.973 ± 0.105
1.751LeuHis: 1.751 ± 0.042
3.956LeuIle: 3.956 ± 0.074
1.547LeuLys: 1.547 ± 0.046
9.911LeuLeu: 9.911 ± 0.132
1.399LeuMet: 1.399 ± 0.038
1.646LeuAsn: 1.646 ± 0.046
5.596LeuPro: 5.596 ± 0.078
2.52LeuGln: 2.52 ± 0.053
7.675LeuArg: 7.675 ± 0.104
5.67LeuSer: 5.67 ± 0.076
6.092LeuThr: 6.092 ± 0.069
9.235LeuVal: 9.235 ± 0.111
1.415LeuTrp: 1.415 ± 0.043
1.701LeuTyr: 1.701 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
1.943MetAla: 1.943 ± 0.042
0.086MetCys: 0.086 ± 0.009
0.779MetAsp: 0.779 ± 0.027
0.56MetGlu: 0.56 ± 0.023
0.468MetPhe: 0.468 ± 0.02
1.208MetGly: 1.208 ± 0.034
0.287MetHis: 0.287 ± 0.015
0.738MetIle: 0.738 ± 0.027
0.343MetLys: 0.343 ± 0.019
1.646MetLeu: 1.646 ± 0.036
0.267MetMet: 0.267 ± 0.018
0.421MetAsn: 0.421 ± 0.019
1.201MetPro: 1.201 ± 0.029
0.47MetGln: 0.47 ± 0.02
1.31MetArg: 1.31 ± 0.036
1.344MetSer: 1.344 ± 0.033
1.647MetThr: 1.647 ± 0.037
1.101MetVal: 1.101 ± 0.034
0.204MetTrp: 0.204 ± 0.014
0.28MetTyr: 0.28 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.383AsnAla: 2.383 ± 0.063
0.124AsnCys: 0.124 ± 0.012
1.136AsnAsp: 1.136 ± 0.036
0.899AsnGlu: 0.899 ± 0.032
0.582AsnPhe: 0.582 ± 0.024
1.818AsnGly: 1.818 ± 0.062
0.311AsnHis: 0.311 ± 0.018
0.731AsnIle: 0.731 ± 0.029
0.324AsnLys: 0.324 ± 0.019
1.782AsnLeu: 1.782 ± 0.045
0.278AsnMet: 0.278 ± 0.018
0.435AsnAsn: 0.435 ± 0.027
1.542AsnPro: 1.542 ± 0.047
0.52AsnGln: 0.52 ± 0.024
1.207AsnArg: 1.207 ± 0.033
0.9AsnSer: 0.9 ± 0.038
1.065AsnThr: 1.065 ± 0.049
1.611AsnVal: 1.611 ± 0.057
0.315AsnTrp: 0.315 ± 0.018
0.443AsnTyr: 0.443 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
7.965ProAla: 7.965 ± 0.109
0.204ProCys: 0.204 ± 0.014
4.387ProAsp: 4.387 ± 0.069
3.826ProGlu: 3.826 ± 0.066
1.79ProPhe: 1.79 ± 0.038
5.512ProGly: 5.512 ± 0.083
1.114ProHis: 1.114 ± 0.028
2.084ProIle: 2.084 ± 0.043
0.93ProLys: 0.93 ± 0.03
5.111ProLeu: 5.111 ± 0.082
0.851ProMet: 0.851 ± 0.029
0.977ProAsn: 0.977 ± 0.028
2.96ProPro: 2.96 ± 0.081
1.514ProGln: 1.514 ± 0.04
3.823ProArg: 3.823 ± 0.074
3.429ProSer: 3.429 ± 0.065
3.426ProThr: 3.426 ± 0.065
5.164ProVal: 5.164 ± 0.075
0.967ProTrp: 0.967 ± 0.034
1.087ProTyr: 1.087 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.418GlnAla: 3.418 ± 0.066
0.093GlnCys: 0.093 ± 0.009
1.277GlnAsp: 1.277 ± 0.032
1.269GlnGlu: 1.269 ± 0.035
0.815GlnPhe: 0.815 ± 0.026
2.077GlnGly: 2.077 ± 0.041
0.573GlnHis: 0.573 ± 0.027
1.273GlnIle: 1.273 ± 0.034
0.588GlnLys: 0.588 ± 0.023
3.045GlnLeu: 3.045 ± 0.055
0.4GlnMet: 0.4 ± 0.021
0.615GlnAsn: 0.615 ± 0.027
1.578GlnPro: 1.578 ± 0.041
1.117GlnGln: 1.117 ± 0.04
2.385GlnArg: 2.385 ± 0.048
1.395GlnSer: 1.395 ± 0.037
1.58GlnThr: 1.58 ± 0.04
2.164GlnVal: 2.164 ± 0.042
0.494GlnTrp: 0.494 ± 0.024
0.585GlnTyr: 0.585 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
9.662ArgAla: 9.662 ± 0.147
0.322ArgCys: 0.322 ± 0.017
4.678ArgAsp: 4.678 ± 0.081
4.495ArgGlu: 4.495 ± 0.079
2.467ArgPhe: 2.467 ± 0.053
5.891ArgGly: 5.891 ± 0.069
1.682ArgHis: 1.682 ± 0.038
3.664ArgIle: 3.664 ± 0.054
1.186ArgLys: 1.186 ± 0.036
7.683ArgLeu: 7.683 ± 0.116
1.613ArgMet: 1.613 ± 0.043
1.286ArgAsn: 1.286 ± 0.034
4.032ArgPro: 4.032 ± 0.087
1.984ArgGln: 1.984 ± 0.049
7.172ArgArg: 7.172 ± 0.128
4.257ArgSer: 4.257 ± 0.07
4.301ArgThr: 4.301 ± 0.074
6.345ArgVal: 6.345 ± 0.088
1.264ArgTrp: 1.264 ± 0.038
1.614ArgTyr: 1.614 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
7.345SerAla: 7.345 ± 0.085
0.256SerCys: 0.256 ± 0.016
3.258SerAsp: 3.258 ± 0.055
2.645SerGlu: 2.645 ± 0.057
1.912SerPhe: 1.912 ± 0.043
6.012SerGly: 6.012 ± 0.106
1.032SerHis: 1.032 ± 0.032
2.587SerIle: 2.587 ± 0.053
0.985SerLys: 0.985 ± 0.032
5.277SerLeu: 5.277 ± 0.073
1.068SerMet: 1.068 ± 0.03
1.013SerAsn: 1.013 ± 0.042
3.359SerPro: 3.359 ± 0.057
1.342SerGln: 1.342 ± 0.035
3.887SerArg: 3.887 ± 0.062
3.509SerSer: 3.509 ± 0.069
3.643SerThr: 3.643 ± 0.08
4.96SerVal: 4.96 ± 0.081
0.932SerTrp: 0.932 ± 0.028
1.156SerTyr: 1.156 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
7.694ThrAla: 7.694 ± 0.116
0.312ThrCys: 0.312 ± 0.02
3.703ThrAsp: 3.703 ± 0.065
2.776ThrGlu: 2.776 ± 0.053
1.898ThrPhe: 1.898 ± 0.041
5.901ThrGly: 5.901 ± 0.102
1.08ThrHis: 1.08 ± 0.027
2.92ThrIle: 2.92 ± 0.066
1.059ThrLys: 1.059 ± 0.03
5.745ThrLeu: 5.745 ± 0.071
0.909ThrMet: 0.909 ± 0.029
1.135ThrAsn: 1.135 ± 0.045
4.004ThrPro: 4.004 ± 0.07
1.464ThrGln: 1.464 ± 0.041
3.775ThrArg: 3.775 ± 0.06
3.402ThrSer: 3.402 ± 0.078
3.925ThrThr: 3.925 ± 0.087
5.705ThrVal: 5.705 ± 0.109
0.896ThrTrp: 0.896 ± 0.031
1.268ThrTyr: 1.268 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
11.916ValAla: 11.916 ± 0.133
0.527ValCys: 0.527 ± 0.023
5.818ValAsp: 5.818 ± 0.068
5.13ValGlu: 5.13 ± 0.075
2.884ValPhe: 2.884 ± 0.049
7.328ValGly: 7.328 ± 0.094
1.649ValHis: 1.649 ± 0.046
4.124ValIle: 4.124 ± 0.064
1.492ValLys: 1.492 ± 0.039
9.112ValLeu: 9.112 ± 0.109
1.349ValMet: 1.349 ± 0.038
1.75ValAsn: 1.75 ± 0.053
4.966ValPro: 4.966 ± 0.082
2.265ValGln: 2.265 ± 0.044
6.482ValArg: 6.482 ± 0.086
5.041ValSer: 5.041 ± 0.08
5.849ValThr: 5.849 ± 0.12
9.419ValVal: 9.419 ± 0.121
1.291ValTrp: 1.291 ± 0.039
1.603ValTyr: 1.603 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
1.729TrpAla: 1.729 ± 0.043
0.097TrpCys: 0.097 ± 0.009
0.881TrpAsp: 0.881 ± 0.027
0.697TrpGlu: 0.697 ± 0.027
0.629TrpPhe: 0.629 ± 0.022
1.204TrpGly: 1.204 ± 0.037
0.344TrpHis: 0.344 ± 0.016
0.778TrpIle: 0.778 ± 0.025
0.288TrpLys: 0.288 ± 0.017
1.773TrpLeu: 1.773 ± 0.049
0.324TrpMet: 0.324 ± 0.017
0.463TrpAsn: 0.463 ± 0.021
0.79TrpPro: 0.79 ± 0.029
0.55TrpGln: 0.55 ± 0.023
1.355TrpArg: 1.355 ± 0.036
0.971TrpSer: 0.971 ± 0.032
1.03TrpThr: 1.03 ± 0.033
1.268TrpVal: 1.268 ± 0.034
0.388TrpTrp: 0.388 ± 0.02
0.343TrpTyr: 0.343 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.488TyrAla: 2.488 ± 0.051
0.116TyrCys: 0.116 ± 0.009
1.434TyrAsp: 1.434 ± 0.042
1.112TyrGlu: 1.112 ± 0.037
0.684TyrPhe: 0.684 ± 0.026
1.926TyrGly: 1.926 ± 0.04
0.317TyrHis: 0.317 ± 0.018
0.741TyrIle: 0.741 ± 0.027
0.295TyrLys: 0.295 ± 0.017
2.099TyrLeu: 2.099 ± 0.045
0.273TyrMet: 0.273 ± 0.016
0.455TyrAsn: 0.455 ± 0.024
1.084TyrPro: 1.084 ± 0.039
0.578TyrGln: 0.578 ± 0.026
1.731TyrArg: 1.731 ± 0.042
1.101TyrSer: 1.101 ± 0.031
1.14TyrThr: 1.14 ± 0.04
1.589TyrVal: 1.589 ± 0.04
0.352TyrTrp: 0.352 ± 0.02
0.485TyrTyr: 0.485 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3319 proteins (1141260 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski