Anolis carolinensis (Green anole) (American chameleon)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Lepidosauria; Squamata;

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1KNL6|G1KNL6_ANOCA Uncharacterized protein OS=Anolis carolinensis OX=28377 PE=4 SV=1
LL1 pKa = 7.34TDD3 pKa = 3.72TEE5 pKa = 4.64VDD7 pKa = 3.43TEE9 pKa = 4.22KK10 pKa = 10.93NQNVEE15 pKa = 4.4GSSSTLEE22 pKa = 4.19LLSDD26 pKa = 3.93IPFTLAPHH34 pKa = 5.9VLAVQDD40 pKa = 3.57TCNDD44 pKa = 3.81FSRR47 pKa = 11.84HH48 pKa = 5.63LLSCDD53 pKa = 3.21VNDD56 pKa = 3.34NLTRR60 pKa = 11.84FWYY63 pKa = 10.18DD64 pKa = 3.17FTLEE68 pKa = 3.96NSVLCEE74 pKa = 3.99SS75 pKa = 4.17

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9GUU8|H9GUU8_ANOCA Protein kinase domain-containing protein OS=Anolis carolinensis OX=28377 GN=LOC103282236 PE=3 SV=1
AA1 pKa = 7.6SPSAQGSILRR11 pKa = 11.84TLTTQPSTAKK21 pKa = 10.31ASPSAQGSILRR32 pKa = 11.84TLTTQPFNAQASPSAQGSILRR53 pKa = 11.84TLTTQPSNAKK63 pKa = 9.96ASPSAQGSILRR74 pKa = 11.84TLTTQPFNAQASPSAQGSILRR95 pKa = 11.84PLTTQPFNAKK105 pKa = 9.92ASPSAQGSILRR116 pKa = 11.84PLTTQPFNAKK126 pKa = 9.92ASPSAQGSVLRR137 pKa = 11.84PLTTQPFNAKK147 pKa = 9.92ASPSAQGSGSILRR160 pKa = 11.84PLTTQPFNAKK170 pKa = 9.92ASPSAQGSILRR181 pKa = 11.84PLTTQPFNAKK191 pKa = 9.92ASPSAQGSILRR202 pKa = 11.84PLTTQPFNAKK212 pKa = 9.92ASPSAQGSVLRR223 pKa = 11.84PLTTQPFNAKK233 pKa = 9.92ASPSAQGSILRR244 pKa = 11.84PLTTQPFNAKK254 pKa = 9.92ASPSAQGSVLRR265 pKa = 11.84PLTTQPFNAKK275 pKa = 9.92ASPSAQGSS283 pKa = 3.61

Molecular weight: 28.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18528

580

19108

10131167

13

8805

530.2

59.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.364 ± 0.017

2.391 ± 0.018

4.804 ± 0.013

7.236 ± 0.022

3.88 ± 0.012

6.194 ± 0.027

2.738 ± 0.012

4.88 ± 0.017

6.424 ± 0.02

9.659 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.237 ± 0.007

4.052 ± 0.013

5.525 ± 0.025

4.701 ± 0.015

5.369 ± 0.015

8.241 ± 0.022

5.365 ± 0.016

5.834 ± 0.014

1.21 ± 0.006

2.891 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski