Bacillus phage Grass

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Nitunavirus; Bacillus virus Grass

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5PTP8|U5PTP8_9CAUD Uncharacterized protein OS=Bacillus phage Grass OX=1406785 GN=Grass_85 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 4.88KK3 pKa = 10.51VKK5 pKa = 10.73AYY7 pKa = 10.84LLDD10 pKa = 3.78NMEE13 pKa = 4.14EE14 pKa = 4.05LKK16 pKa = 11.15DD17 pKa = 3.66VVRR20 pKa = 11.84EE21 pKa = 4.07VNSWNSEE28 pKa = 4.09LEE30 pKa = 4.1HH31 pKa = 8.24LDD33 pKa = 3.38YY34 pKa = 11.05QYY36 pKa = 11.76NDD38 pKa = 3.09EE39 pKa = 5.49DD40 pKa = 3.8FFNTYY45 pKa = 10.41FEE47 pKa = 4.58GEE49 pKa = 3.95PMKK52 pKa = 10.6AVRR55 pKa = 11.84AAVYY59 pKa = 10.75GDD61 pKa = 3.58YY62 pKa = 10.86NYY64 pKa = 10.78TDD66 pKa = 5.04DD67 pKa = 3.83YY68 pKa = 11.8VKK70 pKa = 10.83FDD72 pKa = 3.59GYY74 pKa = 11.84GNLKK78 pKa = 10.37SVSEE82 pKa = 4.1YY83 pKa = 10.92DD84 pKa = 4.01LEE86 pKa = 6.22RR87 pKa = 11.84EE88 pKa = 4.23LEE90 pKa = 4.06DD91 pKa = 4.5HH92 pKa = 6.84IEE94 pKa = 4.24EE95 pKa = 4.96IMDD98 pKa = 3.94SLEE101 pKa = 4.25GNLPNLTLSDD111 pKa = 4.06EE112 pKa = 4.23LTALINEE119 pKa = 4.37EE120 pKa = 4.35EE121 pKa = 4.24EE122 pKa = 4.81GEE124 pKa = 4.15

Molecular weight: 14.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5PY17|U5PY17_9CAUD Uncharacterized protein OS=Bacillus phage Grass OX=1406785 GN=Grass_88 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84GRR5 pKa = 11.84KK6 pKa = 9.19SIRR9 pKa = 11.84PKK11 pKa = 10.0MFQSKK16 pKa = 10.14AATQKK21 pKa = 11.15AMSNFGNALIQRR33 pKa = 11.84TLSAGEE39 pKa = 3.78EE40 pKa = 4.25AVRR43 pKa = 11.84NAQPNDD49 pKa = 3.15VSVTRR54 pKa = 11.84KK55 pKa = 9.41PKK57 pKa = 9.66YY58 pKa = 10.66LEE60 pKa = 3.8VTEE63 pKa = 3.92NRR65 pKa = 11.84LRR67 pKa = 11.84KK68 pKa = 10.05LGVIDD73 pKa = 5.27LKK75 pKa = 11.26PFFAKK80 pKa = 10.24SSKK83 pKa = 10.84KK84 pKa = 10.19KK85 pKa = 8.77MKK87 pKa = 10.43KK88 pKa = 9.68GGGWYY93 pKa = 8.62LTVPITRR100 pKa = 11.84KK101 pKa = 6.87TRR103 pKa = 11.84NMSRR107 pKa = 11.84KK108 pKa = 8.98MYY110 pKa = 10.29DD111 pKa = 2.85QLRR114 pKa = 11.84SVNTRR119 pKa = 11.84PNTKK123 pKa = 8.62KK124 pKa = 9.07TVITDD129 pKa = 3.15YY130 pKa = 11.07LYY132 pKa = 10.94DD133 pKa = 3.61RR134 pKa = 11.84RR135 pKa = 11.84QISDD139 pKa = 3.39STMLNYY145 pKa = 10.36NPRR148 pKa = 11.84SKK150 pKa = 10.96NITKK154 pKa = 10.15EE155 pKa = 3.77RR156 pKa = 11.84VGKK159 pKa = 9.67NRR161 pKa = 11.84HH162 pKa = 5.89SYY164 pKa = 8.95VSYY167 pKa = 9.08RR168 pKa = 11.84TVSDD172 pKa = 3.73KK173 pKa = 11.5SPANSWIINRR183 pKa = 11.84GKK185 pKa = 10.75VNEE188 pKa = 4.42ADD190 pKa = 3.4TSKK193 pKa = 9.79TFIKK197 pKa = 10.82NVDD200 pKa = 3.36RR201 pKa = 11.84LIRR204 pKa = 11.84WQMKK208 pKa = 9.98NNN210 pKa = 3.52

Molecular weight: 24.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

241

0

241

44914

27

1265

186.4

21.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.223 ± 0.208

0.788 ± 0.079

6.595 ± 0.162

8.365 ± 0.338

3.752 ± 0.099

6.361 ± 0.26

1.87 ± 0.114

6.152 ± 0.162

7.423 ± 0.238

8.38 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.094

5.101 ± 0.15

3.202 ± 0.141

3.723 ± 0.133

4.526 ± 0.117

6.301 ± 0.222

6.21 ± 0.231

6.855 ± 0.13

1.049 ± 0.064

4.48 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski