Amino acid dipepetide frequency for Bacillus phage Grass

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.188AlaAla: 5.188 ± 0.539
0.334AlaCys: 0.334 ± 0.086
4.275AlaAsp: 4.275 ± 0.298
4.743AlaGlu: 4.743 ± 0.356
2.293AlaPhe: 2.293 ± 0.211
4.186AlaGly: 4.186 ± 0.512
1.024AlaHis: 1.024 ± 0.143
4.119AlaIle: 4.119 ± 0.334
3.83AlaLys: 3.83 ± 0.401
5.945AlaLeu: 5.945 ± 0.352
1.759AlaMet: 1.759 ± 0.215
2.716AlaAsn: 2.716 ± 0.264
2.137AlaPro: 2.137 ± 0.241
2.627AlaGln: 2.627 ± 0.334
2.672AlaArg: 2.672 ± 0.235
3.162AlaSer: 3.162 ± 0.363
3.852AlaThr: 3.852 ± 0.348
3.919AlaVal: 3.919 ± 0.289
0.69AlaTrp: 0.69 ± 0.129
2.761AlaTyr: 2.761 ± 0.263
0.0AlaXaa: 0.0 ± 0.0
Cys
0.423CysAla: 0.423 ± 0.111
0.245CysCys: 0.245 ± 0.087
0.534CysAsp: 0.534 ± 0.113
0.379CysGlu: 0.379 ± 0.094
0.312CysPhe: 0.312 ± 0.081
0.69CysGly: 0.69 ± 0.147
0.134CysHis: 0.134 ± 0.053
0.445CysIle: 0.445 ± 0.086
0.712CysLys: 0.712 ± 0.128
0.735CysLeu: 0.735 ± 0.127
0.245CysMet: 0.245 ± 0.081
0.423CysAsn: 0.423 ± 0.123
0.356CysPro: 0.356 ± 0.096
0.245CysGln: 0.245 ± 0.079
0.223CysArg: 0.223 ± 0.074
0.601CysSer: 0.601 ± 0.116
0.312CysThr: 0.312 ± 0.075
0.445CysVal: 0.445 ± 0.098
0.089CysTrp: 0.089 ± 0.044
0.334CysTyr: 0.334 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
3.874AspAla: 3.874 ± 0.322
0.534AspCys: 0.534 ± 0.132
3.429AspAsp: 3.429 ± 0.302
4.809AspGlu: 4.809 ± 0.39
3.028AspPhe: 3.028 ± 0.289
4.431AspGly: 4.431 ± 0.387
1.18AspHis: 1.18 ± 0.142
4.698AspIle: 4.698 ± 0.353
4.23AspLys: 4.23 ± 0.362
5.834AspLeu: 5.834 ± 0.372
2.137AspMet: 2.137 ± 0.226
3.006AspAsn: 3.006 ± 0.252
2.293AspPro: 2.293 ± 0.205
2.004AspGln: 2.004 ± 0.211
3.006AspArg: 3.006 ± 0.269
3.83AspSer: 3.83 ± 0.272
4.275AspThr: 4.275 ± 0.318
5.076AspVal: 5.076 ± 0.376
0.802AspTrp: 0.802 ± 0.143
3.473AspTyr: 3.473 ± 0.308
0.0AspXaa: 0.0 ± 0.0
Glu
5.344GluAla: 5.344 ± 0.355
0.579GluCys: 0.579 ± 0.116
6.078GluAsp: 6.078 ± 0.454
9.596GluGlu: 9.596 ± 0.645
2.761GluPhe: 2.761 ± 0.287
5.589GluGly: 5.589 ± 0.347
1.937GluHis: 1.937 ± 0.221
4.698GluIle: 4.698 ± 0.349
5.878GluLys: 5.878 ± 0.423
7.592GluLeu: 7.592 ± 0.424
2.716GluMet: 2.716 ± 0.253
3.741GluAsn: 3.741 ± 0.319
2.249GluPro: 2.249 ± 0.253
2.894GluGln: 2.894 ± 0.265
3.874GluArg: 3.874 ± 0.307
3.674GluSer: 3.674 ± 0.306
3.785GluThr: 3.785 ± 0.299
6.145GluVal: 6.145 ± 0.372
0.957GluTrp: 0.957 ± 0.153
3.562GluTyr: 3.562 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
1.848PheAla: 1.848 ± 0.19
0.49PheCys: 0.49 ± 0.098
2.449PheAsp: 2.449 ± 0.242
2.716PheGlu: 2.716 ± 0.271
1.336PhePhe: 1.336 ± 0.232
2.182PheGly: 2.182 ± 0.22
0.779PheHis: 0.779 ± 0.147
2.828PheIle: 2.828 ± 0.257
2.917PheLys: 2.917 ± 0.238
2.894PheLeu: 2.894 ± 0.262
0.846PheMet: 0.846 ± 0.134
2.249PheAsn: 2.249 ± 0.232
1.314PhePro: 1.314 ± 0.165
1.069PheGln: 1.069 ± 0.169
1.559PheArg: 1.559 ± 0.153
3.006PheSer: 3.006 ± 0.273
2.627PheThr: 2.627 ± 0.25
2.182PheVal: 2.182 ± 0.215
0.49PheTrp: 0.49 ± 0.106
1.737PheTyr: 1.737 ± 0.206
0.0PheXaa: 0.0 ± 0.0
Gly
3.741GlyAla: 3.741 ± 0.518
0.891GlyCys: 0.891 ± 0.142
3.83GlyAsp: 3.83 ± 0.347
4.943GlyGlu: 4.943 ± 0.389
2.293GlyPhe: 2.293 ± 0.224
4.698GlyGly: 4.698 ± 0.621
1.113GlyHis: 1.113 ± 0.155
4.141GlyIle: 4.141 ± 0.306
4.787GlyLys: 4.787 ± 0.342
4.898GlyLeu: 4.898 ± 0.375
1.559GlyMet: 1.559 ± 0.188
3.451GlyAsn: 3.451 ± 0.261
0.712GlyPro: 0.712 ± 0.15
2.115GlyGln: 2.115 ± 0.252
2.739GlyArg: 2.739 ± 0.2
4.431GlySer: 4.431 ± 0.384
4.52GlyThr: 4.52 ± 0.432
4.564GlyVal: 4.564 ± 0.304
0.891GlyTrp: 0.891 ± 0.144
3.295GlyTyr: 3.295 ± 0.299
0.0GlyXaa: 0.0 ± 0.0
His
1.18HisAla: 1.18 ± 0.152
0.2HisCys: 0.2 ± 0.074
0.802HisAsp: 0.802 ± 0.121
0.957HisGlu: 0.957 ± 0.16
0.712HisPhe: 0.712 ± 0.134
1.069HisGly: 1.069 ± 0.159
0.623HisHis: 0.623 ± 0.114
1.536HisIle: 1.536 ± 0.202
1.202HisLys: 1.202 ± 0.194
1.581HisLeu: 1.581 ± 0.191
0.579HisMet: 0.579 ± 0.111
0.868HisAsn: 0.868 ± 0.125
0.757HisPro: 0.757 ± 0.142
0.623HisGln: 0.623 ± 0.126
0.802HisArg: 0.802 ± 0.125
1.336HisSer: 1.336 ± 0.176
0.846HisThr: 0.846 ± 0.134
1.737HisVal: 1.737 ± 0.2
0.223HisTrp: 0.223 ± 0.072
1.069HisTyr: 1.069 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
3.985IleAla: 3.985 ± 0.298
0.512IleCys: 0.512 ± 0.102
4.587IleAsp: 4.587 ± 0.329
5.232IleGlu: 5.232 ± 0.39
1.781IlePhe: 1.781 ± 0.171
3.652IleGly: 3.652 ± 0.336
1.225IleHis: 1.225 ± 0.161
3.451IleIle: 3.451 ± 0.304
5.032IleLys: 5.032 ± 0.314
4.475IleLeu: 4.475 ± 0.328
1.559IleMet: 1.559 ± 0.179
3.496IleAsn: 3.496 ± 0.338
2.293IlePro: 2.293 ± 0.209
2.293IleGln: 2.293 ± 0.192
2.761IleArg: 2.761 ± 0.255
4.253IleSer: 4.253 ± 0.39
4.342IleThr: 4.342 ± 0.448
4.03IleVal: 4.03 ± 0.296
0.468IleTrp: 0.468 ± 0.084
2.093IleTyr: 2.093 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
5.366LysAla: 5.366 ± 0.435
0.512LysCys: 0.512 ± 0.105
4.72LysAsp: 4.72 ± 0.346
7.348LysGlu: 7.348 ± 0.576
2.85LysPhe: 2.85 ± 0.245
4.832LysGly: 4.832 ± 0.339
1.447LysHis: 1.447 ± 0.192
3.34LysIle: 3.34 ± 0.313
6.591LysLys: 6.591 ± 0.587
5.7LysLeu: 5.7 ± 0.36
2.204LysMet: 2.204 ± 0.223
3.318LysAsn: 3.318 ± 0.29
2.338LysPro: 2.338 ± 0.259
3.184LysGln: 3.184 ± 0.286
3.295LysArg: 3.295 ± 0.292
4.364LysSer: 4.364 ± 0.355
3.985LysThr: 3.985 ± 0.245
4.987LysVal: 4.987 ± 0.313
0.891LysTrp: 0.891 ± 0.131
2.961LysTyr: 2.961 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
4.787LeuAla: 4.787 ± 0.333
0.623LeuCys: 0.623 ± 0.122
6.568LeuAsp: 6.568 ± 0.404
7.058LeuGlu: 7.058 ± 0.472
2.872LeuPhe: 2.872 ± 0.262
4.965LeuGly: 4.965 ± 0.305
1.38LeuHis: 1.38 ± 0.174
4.787LeuIle: 4.787 ± 0.332
7.147LeuLys: 7.147 ± 0.509
6.39LeuLeu: 6.39 ± 0.424
1.87LeuMet: 1.87 ± 0.223
4.787LeuAsn: 4.787 ± 0.336
2.85LeuPro: 2.85 ± 0.291
3.162LeuGln: 3.162 ± 0.24
3.696LeuArg: 3.696 ± 0.331
6.101LeuSer: 6.101 ± 0.417
5.433LeuThr: 5.433 ± 0.394
5.076LeuVal: 5.076 ± 0.364
0.824LeuTrp: 0.824 ± 0.143
3.429LeuTyr: 3.429 ± 0.288
0.0LeuXaa: 0.0 ± 0.0
Met
1.803MetAla: 1.803 ± 0.212
0.156MetCys: 0.156 ± 0.053
1.937MetAsp: 1.937 ± 0.219
2.204MetGlu: 2.204 ± 0.223
1.158MetPhe: 1.158 ± 0.174
1.559MetGly: 1.559 ± 0.2
0.445MetHis: 0.445 ± 0.097
1.648MetIle: 1.648 ± 0.187
2.182MetLys: 2.182 ± 0.23
1.937MetLeu: 1.937 ± 0.244
0.401MetMet: 0.401 ± 0.086
1.603MetAsn: 1.603 ± 0.189
0.802MetPro: 0.802 ± 0.124
1.002MetGln: 1.002 ± 0.153
1.113MetArg: 1.113 ± 0.178
2.249MetSer: 2.249 ± 0.216
1.87MetThr: 1.87 ± 0.214
1.113MetVal: 1.113 ± 0.16
0.134MetTrp: 0.134 ± 0.061
1.136MetTyr: 1.136 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
2.761AsnAla: 2.761 ± 0.273
0.468AsnCys: 0.468 ± 0.117
2.761AsnAsp: 2.761 ± 0.243
3.384AsnGlu: 3.384 ± 0.278
1.648AsnPhe: 1.648 ± 0.222
3.941AsnGly: 3.941 ± 0.337
1.069AsnHis: 1.069 ± 0.153
3.295AsnIle: 3.295 ± 0.326
4.052AsnLys: 4.052 ± 0.358
4.23AsnLeu: 4.23 ± 0.256
1.314AsnMet: 1.314 ± 0.174
2.85AsnAsn: 2.85 ± 0.28
2.36AsnPro: 2.36 ± 0.238
1.625AsnGln: 1.625 ± 0.2
2.694AsnArg: 2.694 ± 0.26
3.251AsnSer: 3.251 ± 0.29
3.162AsnThr: 3.162 ± 0.253
3.496AsnVal: 3.496 ± 0.217
0.534AsnTrp: 0.534 ± 0.109
2.115AsnTyr: 2.115 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
2.382ProAla: 2.382 ± 0.369
0.245ProCys: 0.245 ± 0.074
2.271ProAsp: 2.271 ± 0.227
3.05ProGlu: 3.05 ± 0.283
1.136ProPhe: 1.136 ± 0.184
1.002ProGly: 1.002 ± 0.139
0.712ProHis: 0.712 ± 0.116
1.937ProIle: 1.937 ± 0.208
2.093ProLys: 2.093 ± 0.238
2.85ProLeu: 2.85 ± 0.247
0.623ProMet: 0.623 ± 0.125
1.759ProAsn: 1.759 ± 0.182
1.002ProPro: 1.002 ± 0.156
1.225ProGln: 1.225 ± 0.186
1.091ProArg: 1.091 ± 0.174
2.048ProSer: 2.048 ± 0.209
2.561ProThr: 2.561 ± 0.242
2.16ProVal: 2.16 ± 0.195
0.223ProTrp: 0.223 ± 0.073
1.648ProTyr: 1.648 ± 0.199
0.0ProXaa: 0.0 ± 0.0
Gln
2.382GlnAla: 2.382 ± 0.248
0.223GlnCys: 0.223 ± 0.082
2.093GlnAsp: 2.093 ± 0.189
3.763GlnGlu: 3.763 ± 0.309
1.336GlnPhe: 1.336 ± 0.194
1.67GlnGly: 1.67 ± 0.192
0.579GlnHis: 0.579 ± 0.107
2.293GlnIle: 2.293 ± 0.261
2.605GlnLys: 2.605 ± 0.238
3.407GlnLeu: 3.407 ± 0.268
1.358GlnMet: 1.358 ± 0.162
1.937GlnAsn: 1.937 ± 0.247
1.225GlnPro: 1.225 ± 0.261
2.004GlnGln: 2.004 ± 0.263
1.982GlnArg: 1.982 ± 0.203
2.382GlnSer: 2.382 ± 0.263
1.403GlnThr: 1.403 ± 0.176
2.716GlnVal: 2.716 ± 0.212
0.379GlnTrp: 0.379 ± 0.096
1.492GlnTyr: 1.492 ± 0.186
0.0GlnXaa: 0.0 ± 0.0
Arg
2.427ArgAla: 2.427 ± 0.224
0.267ArgCys: 0.267 ± 0.087
2.382ArgAsp: 2.382 ± 0.245
4.253ArgGlu: 4.253 ± 0.284
1.937ArgPhe: 1.937 ± 0.195
3.206ArgGly: 3.206 ± 0.266
0.802ArgHis: 0.802 ± 0.128
3.162ArgIle: 3.162 ± 0.274
3.674ArgLys: 3.674 ± 0.345
4.075ArgLeu: 4.075 ± 0.311
1.403ArgMet: 1.403 ± 0.214
1.826ArgAsn: 1.826 ± 0.24
1.136ArgPro: 1.136 ± 0.146
1.959ArgGln: 1.959 ± 0.211
1.937ArgArg: 1.937 ± 0.227
2.316ArgSer: 2.316 ± 0.191
2.716ArgThr: 2.716 ± 0.267
3.318ArgVal: 3.318 ± 0.264
0.379ArgTrp: 0.379 ± 0.095
2.093ArgTyr: 2.093 ± 0.241
0.0ArgXaa: 0.0 ± 0.0
Ser
3.318SerAla: 3.318 ± 0.345
0.356SerCys: 0.356 ± 0.118
3.741SerAsp: 3.741 ± 0.271
4.52SerGlu: 4.52 ± 0.324
3.117SerPhe: 3.117 ± 0.233
4.141SerGly: 4.141 ± 0.346
1.002SerHis: 1.002 ± 0.145
3.852SerIle: 3.852 ± 0.296
5.166SerLys: 5.166 ± 0.379
5.21SerLeu: 5.21 ± 0.31
1.803SerMet: 1.803 ± 0.22
3.384SerAsn: 3.384 ± 0.349
1.737SerPro: 1.737 ± 0.196
2.271SerGln: 2.271 ± 0.262
2.605SerArg: 2.605 ± 0.219
4.787SerSer: 4.787 ± 0.385
3.985SerThr: 3.985 ± 0.366
4.431SerVal: 4.431 ± 0.3
0.735SerTrp: 0.735 ± 0.143
2.85SerTyr: 2.85 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
4.008ThrAla: 4.008 ± 0.365
0.312ThrCys: 0.312 ± 0.079
4.141ThrAsp: 4.141 ± 0.412
4.676ThrGlu: 4.676 ± 0.316
2.204ThrPhe: 2.204 ± 0.217
4.431ThrGly: 4.431 ± 0.41
1.024ThrHis: 1.024 ± 0.156
4.008ThrIle: 4.008 ± 0.36
3.763ThrLys: 3.763 ± 0.244
5.722ThrLeu: 5.722 ± 0.429
1.291ThrMet: 1.291 ± 0.167
2.783ThrAsn: 2.783 ± 0.324
2.605ThrPro: 2.605 ± 0.232
2.338ThrGln: 2.338 ± 0.163
2.872ThrArg: 2.872 ± 0.229
3.228ThrSer: 3.228 ± 0.298
4.119ThrThr: 4.119 ± 0.359
4.965ThrVal: 4.965 ± 0.338
0.534ThrTrp: 0.534 ± 0.116
3.073ThrTyr: 3.073 ± 0.291
0.0ThrXaa: 0.0 ± 0.0
Val
4.186ValAla: 4.186 ± 0.312
0.468ValCys: 0.468 ± 0.108
5.344ValAsp: 5.344 ± 0.345
5.566ValGlu: 5.566 ± 0.36
2.85ValPhe: 2.85 ± 0.253
3.785ValGly: 3.785 ± 0.24
1.314ValHis: 1.314 ± 0.174
3.674ValIle: 3.674 ± 0.278
4.564ValLys: 4.564 ± 0.316
5.321ValLeu: 5.321 ± 0.345
1.581ValMet: 1.581 ± 0.2
3.54ValAsn: 3.54 ± 0.283
2.561ValPro: 2.561 ± 0.256
2.627ValGln: 2.627 ± 0.187
3.318ValArg: 3.318 ± 0.266
4.743ValSer: 4.743 ± 0.35
4.787ValThr: 4.787 ± 0.419
4.431ValVal: 4.431 ± 0.324
0.646ValTrp: 0.646 ± 0.13
3.251ValTyr: 3.251 ± 0.276
0.0ValXaa: 0.0 ± 0.0
Trp
0.557TrpAla: 0.557 ± 0.114
0.134TrpCys: 0.134 ± 0.058
0.868TrpAsp: 0.868 ± 0.141
1.136TrpGlu: 1.136 ± 0.18
0.423TrpPhe: 0.423 ± 0.101
0.712TrpGly: 0.712 ± 0.126
0.2TrpHis: 0.2 ± 0.069
0.534TrpIle: 0.534 ± 0.108
0.735TrpLys: 0.735 ± 0.123
0.668TrpLeu: 0.668 ± 0.124
0.2TrpMet: 0.2 ± 0.078
0.49TrpAsn: 0.49 ± 0.108
0.0TrpPro: 0.0 ± 0.0
0.267TrpGln: 0.267 ± 0.098
0.557TrpArg: 0.557 ± 0.103
0.579TrpSer: 0.579 ± 0.134
0.668TrpThr: 0.668 ± 0.115
0.98TrpVal: 0.98 ± 0.127
0.178TrpTrp: 0.178 ± 0.069
0.601TrpTyr: 0.601 ± 0.117
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.672TyrAla: 2.672 ± 0.26
0.334TyrCys: 0.334 ± 0.084
3.139TyrAsp: 3.139 ± 0.262
3.318TyrGlu: 3.318 ± 0.252
1.47TyrPhe: 1.47 ± 0.185
2.872TyrGly: 2.872 ± 0.265
0.712TyrHis: 0.712 ± 0.135
3.073TyrIle: 3.073 ± 0.259
3.073TyrLys: 3.073 ± 0.246
4.342TyrLeu: 4.342 ± 0.36
0.98TyrMet: 0.98 ± 0.14
2.783TyrAsn: 2.783 ± 0.26
1.291TyrPro: 1.291 ± 0.183
1.692TyrGln: 1.692 ± 0.198
2.471TyrArg: 2.471 ± 0.191
2.672TyrSer: 2.672 ± 0.267
2.85TyrThr: 2.85 ± 0.289
2.783TyrVal: 2.783 ± 0.257
0.423TyrTrp: 0.423 ± 0.095
1.826TyrTyr: 1.826 ± 0.188
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 241 proteins (44914 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski