Thiohalomonas denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Thiohalomonas

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5PQV2|A0A1G5PQV2_9GAMM Osmotically-inducible protein OsmY contains BON domain OS=Thiohalomonas denitrificans OX=415747 GN=SAMN03097708_00599 PE=4 SV=1
MM1 pKa = 6.41MTSLSAQRR9 pKa = 11.84GYY11 pKa = 9.91ATLVVSLILLVAITLITLYY30 pKa = 10.68GARR33 pKa = 11.84TSLMTQRR40 pKa = 11.84ILGNDD45 pKa = 2.96KK46 pKa = 10.41RR47 pKa = 11.84ATEE50 pKa = 4.21SFVEE54 pKa = 4.25AEE56 pKa = 4.1RR57 pKa = 11.84GLDD60 pKa = 3.39EE61 pKa = 4.17GAMFISRR68 pKa = 11.84NIRR71 pKa = 11.84DD72 pKa = 4.14LSNAWVTEE80 pKa = 4.14TRR82 pKa = 11.84WVSCGTSIEE91 pKa = 4.38LPCGDD96 pKa = 3.64GSQNIFDD103 pKa = 4.72ANFVYY108 pKa = 10.87YY109 pKa = 10.02QDD111 pKa = 4.92PDD113 pKa = 4.4DD114 pKa = 3.88NSKK117 pKa = 11.39NYY119 pKa = 9.58VAEE122 pKa = 4.44LSDD125 pKa = 3.88DD126 pKa = 3.74EE127 pKa = 4.29PRR129 pKa = 11.84RR130 pKa = 11.84VYY132 pKa = 10.64LVARR136 pKa = 11.84CQDD139 pKa = 3.53EE140 pKa = 4.67DD141 pKa = 4.61PPDD144 pKa = 4.03GTCDD148 pKa = 3.28HH149 pKa = 6.97VGPGAYY155 pKa = 9.33TIDD158 pKa = 4.66PDD160 pKa = 4.33DD161 pKa = 5.69HH162 pKa = 8.51DD163 pKa = 5.99DD164 pKa = 3.52EE165 pKa = 5.9DD166 pKa = 3.89MRR168 pKa = 11.84GSLIHH173 pKa = 6.77TIATGTSDD181 pKa = 5.1DD182 pKa = 4.04GTGSATIRR190 pKa = 11.84QSHH193 pKa = 6.7YY194 pKa = 10.02IYY196 pKa = 10.61QLVASSPASPLTIDD210 pKa = 3.39AASNVSGSFEE220 pKa = 4.09IVTNPDD226 pKa = 2.81GGGTGVPVSMWSDD239 pKa = 3.42QPIDD243 pKa = 3.51VGTAITTCEE252 pKa = 3.82VGEE255 pKa = 4.23YY256 pKa = 9.98EE257 pKa = 5.46LNDD260 pKa = 3.83GSCPGTTPEE269 pKa = 4.9SLTADD274 pKa = 3.53GDD276 pKa = 3.9AGIDD280 pKa = 3.24IVGEE284 pKa = 4.11ANPFPADD291 pKa = 3.03MFDD294 pKa = 3.79YY295 pKa = 11.04VFGIPTEE302 pKa = 4.38EE303 pKa = 3.93YY304 pKa = 10.92NQVKK308 pKa = 10.18DD309 pKa = 3.27QATAAGHH316 pKa = 7.26LLTDD320 pKa = 4.65CSTLDD325 pKa = 3.4TASNGLYY332 pKa = 9.53WIEE335 pKa = 4.08GACDD339 pKa = 3.46LKK341 pKa = 11.09IEE343 pKa = 4.65IGSSAGPVLLVIADD357 pKa = 3.82GQLDD361 pKa = 3.68INAGAEE367 pKa = 4.38VYY369 pKa = 10.74GLVYY373 pKa = 10.8AFDD376 pKa = 4.19VPPEE380 pKa = 4.77DD381 pKa = 4.1DD382 pKa = 4.82AGDD385 pKa = 3.34IKK387 pKa = 11.4LNGGALIRR395 pKa = 11.84GGFVADD401 pKa = 5.48RR402 pKa = 11.84KK403 pKa = 8.61PTTNGAMTIYY413 pKa = 10.7YY414 pKa = 10.35DD415 pKa = 4.26ADD417 pKa = 3.87LLEE420 pKa = 4.51KK421 pKa = 10.79LVDD424 pKa = 3.93NEE426 pKa = 4.15SLAASGRR433 pKa = 11.84VPGSWVDD440 pKa = 3.2YY441 pKa = 11.26HH442 pKa = 6.27EE443 pKa = 4.39

Molecular weight: 47.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5QX78|A0A1G5QX78_9GAMM Uncharacterized protein OS=Thiohalomonas denitrificans OX=415747 GN=SAMN03097708_02889 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.2RR14 pKa = 11.84THH16 pKa = 6.02GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTVGGRR28 pKa = 11.84KK29 pKa = 8.1VLKK32 pKa = 10.25ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.31GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3255

0

3255

1022376

24

4124

314.1

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.118 ± 0.041

0.921 ± 0.016

5.61 ± 0.036

7.268 ± 0.051

3.687 ± 0.029

8.211 ± 0.039

2.397 ± 0.026

4.932 ± 0.032

3.242 ± 0.039

10.664 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.021

2.739 ± 0.022

4.946 ± 0.03

3.463 ± 0.026

7.583 ± 0.051

5.421 ± 0.033

5.009 ± 0.043

7.409 ± 0.04

1.389 ± 0.021

2.605 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski