Synechococcus phage S-T4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tamkungvirus; Synechococcus virus ST4

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385EGV6|A0A385EGV6_9CAUD Photosystem II OS=Synechococcus phage S-T4 OX=2268578 PE=3 SV=1
MM1 pKa = 7.66ISDD4 pKa = 3.91ATYY7 pKa = 10.94QDD9 pKa = 4.81WEE11 pKa = 4.49DD12 pKa = 3.13FWYY15 pKa = 10.89NGEE18 pKa = 4.42AEE20 pKa = 4.36QQEE23 pKa = 4.59PMTDD27 pKa = 3.42LSDD30 pKa = 3.27LHH32 pKa = 6.9YY33 pKa = 11.05EE34 pKa = 4.5RR35 pKa = 11.84IAFLEE40 pKa = 4.06SEE42 pKa = 3.93IVKK45 pKa = 9.7YY46 pKa = 9.4QEE48 pKa = 4.67EE49 pKa = 4.14ISLLKK54 pKa = 10.62DD55 pKa = 3.17QIEE58 pKa = 4.54IISNGRR64 pKa = 11.84EE65 pKa = 3.85YY66 pKa = 11.3DD67 pKa = 3.62CC68 pKa = 6.34

Molecular weight: 8.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385EGX6|A0A385EGX6_9CAUD Uncharacterized protein OS=Synechococcus phage S-T4 OX=2268578 PE=4 SV=1
MM1 pKa = 7.18VGSASTGFVPNRR13 pKa = 11.84QVPIRR18 pKa = 11.84VEE20 pKa = 3.94LKK22 pKa = 10.87AFFQFVNSFFMNFSQILFKK41 pKa = 11.21VV42 pKa = 3.44

Molecular weight: 4.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

202

0

202

51871

38

4749

256.8

28.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.957 ± 0.249

0.941 ± 0.091

6.628 ± 0.135

6.35 ± 0.304

4.766 ± 0.13

7.684 ± 0.383

1.623 ± 0.133

6.915 ± 0.217

6.053 ± 0.379

7.256 ± 0.169

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.974 ± 0.188

5.868 ± 0.16

3.946 ± 0.141

3.462 ± 0.121

4.022 ± 0.133

7.361 ± 0.276

7.125 ± 0.421

6.676 ± 0.227

1.134 ± 0.122

4.257 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski