Amino acid dipepetide frequency for Synechococcus phage S-T4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.415AlaAla: 4.415 ± 0.448
0.54AlaCys: 0.54 ± 0.139
3.297AlaAsp: 3.297 ± 0.296
3.393AlaGlu: 3.393 ± 0.279
2.371AlaPhe: 2.371 ± 0.224
5.205AlaGly: 5.205 ± 0.452
1.022AlaHis: 1.022 ± 0.182
3.991AlaIle: 3.991 ± 0.298
3.123AlaLys: 3.123 ± 0.285
4.241AlaLeu: 4.241 ± 0.413
1.041AlaMet: 1.041 ± 0.171
3.489AlaAsn: 3.489 ± 0.305
2.699AlaPro: 2.699 ± 0.235
2.506AlaGln: 2.506 ± 0.278
2.179AlaArg: 2.179 ± 0.259
5.225AlaSer: 5.225 ± 0.317
4.569AlaThr: 4.569 ± 0.543
3.837AlaVal: 3.837 ± 0.301
0.521AlaTrp: 0.521 ± 0.112
1.909AlaTyr: 1.909 ± 0.2
0.0AlaXaa: 0.0 ± 0.0
Cys
0.636CysAla: 0.636 ± 0.102
0.058CysCys: 0.058 ± 0.033
0.655CysAsp: 0.655 ± 0.132
0.636CysGlu: 0.636 ± 0.131
0.405CysPhe: 0.405 ± 0.105
0.598CysGly: 0.598 ± 0.143
0.251CysHis: 0.251 ± 0.072
0.559CysIle: 0.559 ± 0.109
0.655CysLys: 0.655 ± 0.143
0.733CysLeu: 0.733 ± 0.12
0.366CysMet: 0.366 ± 0.1
0.405CysAsn: 0.405 ± 0.096
0.501CysPro: 0.501 ± 0.134
0.308CysGln: 0.308 ± 0.069
0.463CysArg: 0.463 ± 0.105
0.636CysSer: 0.636 ± 0.129
0.521CysThr: 0.521 ± 0.106
0.636CysVal: 0.636 ± 0.116
0.116CysTrp: 0.116 ± 0.047
0.27CysTyr: 0.27 ± 0.069
0.0CysXaa: 0.0 ± 0.0
Asp
3.663AspAla: 3.663 ± 0.312
0.694AspCys: 0.694 ± 0.13
4.357AspAsp: 4.357 ± 0.351
3.856AspGlu: 3.856 ± 0.296
3.798AspPhe: 3.798 ± 0.26
5.302AspGly: 5.302 ± 0.307
1.176AspHis: 1.176 ± 0.154
4.935AspIle: 4.935 ± 0.452
3.567AspLys: 3.567 ± 0.331
5.938AspLeu: 5.938 ± 0.378
1.369AspMet: 1.369 ± 0.19
3.277AspAsn: 3.277 ± 0.277
3.142AspPro: 3.142 ± 0.238
2.275AspGln: 2.275 ± 0.188
2.583AspArg: 2.583 ± 0.277
3.856AspSer: 3.856 ± 0.289
4.01AspThr: 4.01 ± 0.378
3.971AspVal: 3.971 ± 0.266
1.08AspTrp: 1.08 ± 0.192
3.432AspTyr: 3.432 ± 0.29
0.0AspXaa: 0.0 ± 0.0
Glu
3.142GluAla: 3.142 ± 0.275
0.617GluCys: 0.617 ± 0.112
4.338GluAsp: 4.338 ± 0.369
4.704GluGlu: 4.704 ± 0.478
3.451GluPhe: 3.451 ± 0.324
4.164GluGly: 4.164 ± 0.347
1.06GluHis: 1.06 ± 0.167
4.839GluIle: 4.839 ± 0.384
4.434GluLys: 4.434 ± 0.518
5.147GluLeu: 5.147 ± 0.415
1.581GluMet: 1.581 ± 0.23
3.624GluAsn: 3.624 ± 0.326
2.005GluPro: 2.005 ± 0.215
2.236GluGln: 2.236 ± 0.259
2.41GluArg: 2.41 ± 0.216
4.164GluSer: 4.164 ± 0.347
3.432GluThr: 3.432 ± 0.264
4.608GluVal: 4.608 ± 0.359
0.81GluTrp: 0.81 ± 0.161
2.738GluTyr: 2.738 ± 0.269
0.0GluXaa: 0.0 ± 0.0
Phe
2.853PheAla: 2.853 ± 0.245
0.463PheCys: 0.463 ± 0.114
3.894PheAsp: 3.894 ± 0.25
2.988PheGlu: 2.988 ± 0.286
1.928PhePhe: 1.928 ± 0.215
3.335PheGly: 3.335 ± 0.333
0.636PheHis: 0.636 ± 0.125
2.911PheIle: 2.911 ± 0.221
2.66PheLys: 2.66 ± 0.193
3.046PheLeu: 3.046 ± 0.261
1.099PheMet: 1.099 ± 0.167
3.374PheAsn: 3.374 ± 0.357
1.986PhePro: 1.986 ± 0.202
1.581PheGln: 1.581 ± 0.169
1.735PheArg: 1.735 ± 0.157
3.894PheSer: 3.894 ± 0.351
3.509PheThr: 3.509 ± 0.28
3.586PheVal: 3.586 ± 0.31
0.482PheTrp: 0.482 ± 0.11
1.697PheTyr: 1.697 ± 0.187
0.0PheXaa: 0.0 ± 0.0
Gly
5.032GlyAla: 5.032 ± 0.493
0.636GlyCys: 0.636 ± 0.12
4.762GlyAsp: 4.762 ± 0.428
4.588GlyGlu: 4.588 ± 0.348
3.624GlyPhe: 3.624 ± 0.41
6.131GlyGly: 6.131 ± 0.815
1.157GlyHis: 1.157 ± 0.242
6.401GlyIle: 6.401 ± 0.887
4.029GlyLys: 4.029 ± 0.373
4.916GlyLeu: 4.916 ± 0.344
1.33GlyMet: 1.33 ± 0.17
4.376GlyAsn: 4.376 ± 0.413
2.005GlyPro: 2.005 ± 0.222
2.294GlyGln: 2.294 ± 0.216
2.93GlyArg: 2.93 ± 0.314
6.458GlySer: 6.458 ± 0.517
5.764GlyThr: 5.764 ± 0.771
5.649GlyVal: 5.649 ± 0.561
1.234GlyTrp: 1.234 ± 0.172
3.528GlyTyr: 3.528 ± 0.331
0.0GlyXaa: 0.0 ± 0.0
His
0.79HisAla: 0.79 ± 0.112
0.174HisCys: 0.174 ± 0.066
0.906HisAsp: 0.906 ± 0.159
1.137HisGlu: 1.137 ± 0.207
0.887HisPhe: 0.887 ± 0.142
1.157HisGly: 1.157 ± 0.222
0.424HisHis: 0.424 ± 0.088
1.06HisIle: 1.06 ± 0.162
1.137HisLys: 1.137 ± 0.179
1.118HisLeu: 1.118 ± 0.175
0.193HisMet: 0.193 ± 0.06
0.925HisAsn: 0.925 ± 0.225
0.906HisPro: 0.906 ± 0.167
0.54HisGln: 0.54 ± 0.105
0.733HisArg: 0.733 ± 0.13
0.983HisSer: 0.983 ± 0.146
1.137HisThr: 1.137 ± 0.173
0.81HisVal: 0.81 ± 0.145
0.231HisTrp: 0.231 ± 0.068
0.983HisTyr: 0.983 ± 0.186
0.0HisXaa: 0.0 ± 0.0
Ile
3.991IleAla: 3.991 ± 0.252
0.636IleCys: 0.636 ± 0.102
5.225IleAsp: 5.225 ± 0.449
4.704IleGlu: 4.704 ± 0.325
2.776IlePhe: 2.776 ± 0.223
5.302IleGly: 5.302 ± 0.584
0.868IleHis: 0.868 ± 0.168
4.434IleIle: 4.434 ± 0.351
4.453IleLys: 4.453 ± 0.339
4.762IleLeu: 4.762 ± 0.266
1.157IleMet: 1.157 ± 0.172
4.28IleAsn: 4.28 ± 0.272
2.699IlePro: 2.699 ± 0.223
2.487IleGln: 2.487 ± 0.305
3.065IleArg: 3.065 ± 0.273
5.572IleSer: 5.572 ± 0.583
5.34IleThr: 5.34 ± 0.559
4.434IleVal: 4.434 ± 0.409
0.598IleTrp: 0.598 ± 0.138
2.371IleTyr: 2.371 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
3.142LysAla: 3.142 ± 0.343
0.578LysCys: 0.578 ± 0.135
3.547LysAsp: 3.547 ± 0.285
4.241LysGlu: 4.241 ± 0.5
2.815LysPhe: 2.815 ± 0.255
2.988LysGly: 2.988 ± 0.256
0.81LysHis: 0.81 ± 0.164
4.241LysIle: 4.241 ± 0.288
5.437LysLys: 5.437 ± 0.629
4.723LysLeu: 4.723 ± 0.418
1.484LysMet: 1.484 ± 0.239
3.605LysAsn: 3.605 ± 0.248
2.217LysPro: 2.217 ± 0.233
1.947LysGln: 1.947 ± 0.264
2.506LysArg: 2.506 ± 0.331
4.184LysSer: 4.184 ± 0.424
4.01LysThr: 4.01 ± 0.254
4.145LysVal: 4.145 ± 0.317
0.694LysTrp: 0.694 ± 0.156
3.22LysTyr: 3.22 ± 0.363
0.0LysXaa: 0.0 ± 0.0
Leu
4.299LeuAla: 4.299 ± 0.307
0.733LeuCys: 0.733 ± 0.134
6.034LeuAsp: 6.034 ± 0.37
4.762LeuGlu: 4.762 ± 0.372
3.239LeuPhe: 3.239 ± 0.258
4.743LeuGly: 4.743 ± 0.33
1.407LeuHis: 1.407 ± 0.173
4.164LeuIle: 4.164 ± 0.31
4.916LeuLys: 4.916 ± 0.369
4.434LeuLeu: 4.434 ± 0.36
1.523LeuMet: 1.523 ± 0.217
4.781LeuAsn: 4.781 ± 0.286
3.47LeuPro: 3.47 ± 0.273
2.506LeuGln: 2.506 ± 0.205
3.258LeuArg: 3.258 ± 0.278
5.707LeuSer: 5.707 ± 0.366
4.839LeuThr: 4.839 ± 0.351
4.145LeuVal: 4.145 ± 0.306
0.79LeuTrp: 0.79 ± 0.145
2.795LeuTyr: 2.795 ± 0.248
0.0LeuXaa: 0.0 ± 0.0
Met
1.581MetAla: 1.581 ± 0.239
0.212MetCys: 0.212 ± 0.077
1.099MetAsp: 1.099 ± 0.184
1.215MetGlu: 1.215 ± 0.193
0.713MetPhe: 0.713 ± 0.136
0.983MetGly: 0.983 ± 0.191
0.27MetHis: 0.27 ± 0.091
1.446MetIle: 1.446 ± 0.244
1.697MetLys: 1.697 ± 0.279
1.157MetLeu: 1.157 ± 0.18
0.598MetMet: 0.598 ± 0.144
1.195MetAsn: 1.195 ± 0.174
0.868MetPro: 0.868 ± 0.13
0.752MetGln: 0.752 ± 0.123
1.003MetArg: 1.003 ± 0.159
1.504MetSer: 1.504 ± 0.197
1.311MetThr: 1.311 ± 0.184
1.099MetVal: 1.099 ± 0.167
0.251MetTrp: 0.251 ± 0.076
0.79MetTyr: 0.79 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
3.162AsnAla: 3.162 ± 0.23
0.559AsnCys: 0.559 ± 0.121
3.104AsnAsp: 3.104 ± 0.244
3.046AsnGlu: 3.046 ± 0.26
3.181AsnPhe: 3.181 ± 0.301
4.55AsnGly: 4.55 ± 0.359
1.195AsnHis: 1.195 ± 0.182
4.666AsnIle: 4.666 ± 0.345
3.027AsnLys: 3.027 ± 0.292
5.07AsnLeu: 5.07 ± 0.287
0.983AsnMet: 0.983 ± 0.152
3.528AsnAsn: 3.528 ± 0.304
3.412AsnPro: 3.412 ± 0.331
1.966AsnGln: 1.966 ± 0.18
2.448AsnArg: 2.448 ± 0.195
4.184AsnSer: 4.184 ± 0.324
3.509AsnThr: 3.509 ± 0.377
4.434AsnVal: 4.434 ± 0.421
0.54AsnTrp: 0.54 ± 0.117
2.121AsnTyr: 2.121 ± 0.228
0.0AsnXaa: 0.0 ± 0.0
Pro
2.14ProAla: 2.14 ± 0.316
0.308ProCys: 0.308 ± 0.081
2.892ProAsp: 2.892 ± 0.247
2.911ProGlu: 2.911 ± 0.316
1.832ProPhe: 1.832 ± 0.215
2.873ProGly: 2.873 ± 0.204
0.964ProHis: 0.964 ± 0.131
2.603ProIle: 2.603 ± 0.228
2.313ProLys: 2.313 ± 0.245
2.333ProLeu: 2.333 ± 0.173
0.617ProMet: 0.617 ± 0.122
2.14ProAsn: 2.14 ± 0.192
1.446ProPro: 1.446 ± 0.156
1.658ProGln: 1.658 ± 0.19
1.195ProArg: 1.195 ± 0.215
2.969ProSer: 2.969 ± 0.269
3.682ProThr: 3.682 ± 0.236
2.718ProVal: 2.718 ± 0.262
0.501ProTrp: 0.501 ± 0.116
1.369ProTyr: 1.369 ± 0.141
0.0ProXaa: 0.0 ± 0.0
Gln
1.6GlnAla: 1.6 ± 0.243
0.251GlnCys: 0.251 ± 0.08
1.947GlnAsp: 1.947 ± 0.17
2.41GlnGlu: 2.41 ± 0.26
1.793GlnPhe: 1.793 ± 0.208
2.101GlnGly: 2.101 ± 0.197
0.463GlnHis: 0.463 ± 0.113
2.93GlnIle: 2.93 ± 0.218
2.082GlnLys: 2.082 ± 0.255
2.95GlnLeu: 2.95 ± 0.255
0.81GlnMet: 0.81 ± 0.157
1.658GlnAsn: 1.658 ± 0.167
1.446GlnPro: 1.446 ± 0.229
1.504GlnGln: 1.504 ± 0.233
1.639GlnArg: 1.639 ± 0.19
2.622GlnSer: 2.622 ± 0.302
2.275GlnThr: 2.275 ± 0.228
2.024GlnVal: 2.024 ± 0.188
0.366GlnTrp: 0.366 ± 0.11
1.754GlnTyr: 1.754 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
2.159ArgAla: 2.159 ± 0.275
0.27ArgCys: 0.27 ± 0.078
2.429ArgAsp: 2.429 ± 0.204
2.892ArgGlu: 2.892 ± 0.335
2.101ArgPhe: 2.101 ± 0.178
3.258ArgGly: 3.258 ± 0.219
0.598ArgHis: 0.598 ± 0.136
2.545ArgIle: 2.545 ± 0.219
2.93ArgLys: 2.93 ± 0.355
3.355ArgLeu: 3.355 ± 0.259
0.945ArgMet: 0.945 ± 0.167
2.256ArgAsn: 2.256 ± 0.208
1.388ArgPro: 1.388 ± 0.156
1.099ArgGln: 1.099 ± 0.164
1.928ArgArg: 1.928 ± 0.259
2.545ArgSer: 2.545 ± 0.193
2.14ArgThr: 2.14 ± 0.292
2.487ArgVal: 2.487 ± 0.319
0.598ArgTrp: 0.598 ± 0.128
2.294ArgTyr: 2.294 ± 0.281
0.0ArgXaa: 0.0 ± 0.0
Ser
4.569SerAla: 4.569 ± 0.334
0.578SerCys: 0.578 ± 0.096
4.8SerAsp: 4.8 ± 0.327
4.029SerGlu: 4.029 ± 0.271
3.837SerPhe: 3.837 ± 0.259
8.097SerGly: 8.097 ± 0.923
1.137SerHis: 1.137 ± 0.157
4.993SerIle: 4.993 ± 0.426
3.489SerLys: 3.489 ± 0.258
5.514SerLeu: 5.514 ± 0.36
1.446SerMet: 1.446 ± 0.204
3.779SerAsn: 3.779 ± 0.226
2.294SerPro: 2.294 ± 0.215
2.371SerGln: 2.371 ± 0.202
2.718SerArg: 2.718 ± 0.225
5.34SerSer: 5.34 ± 0.324
5.996SerThr: 5.996 ± 0.832
4.8SerVal: 4.8 ± 0.342
0.79SerTrp: 0.79 ± 0.13
3.027SerTyr: 3.027 ± 0.293
0.0SerXaa: 0.0 ± 0.0
Thr
5.398ThrAla: 5.398 ± 0.675
0.752ThrCys: 0.752 ± 0.134
4.126ThrAsp: 4.126 ± 0.313
3.817ThrGlu: 3.817 ± 0.335
3.779ThrPhe: 3.779 ± 0.335
6.728ThrGly: 6.728 ± 0.801
0.81ThrHis: 0.81 ± 0.137
5.495ThrIle: 5.495 ± 0.633
3.971ThrLys: 3.971 ± 0.265
4.762ThrLeu: 4.762 ± 0.368
0.81ThrMet: 0.81 ± 0.141
4.511ThrAsn: 4.511 ± 0.44
2.815ThrPro: 2.815 ± 0.238
2.063ThrGln: 2.063 ± 0.211
2.41ThrArg: 2.41 ± 0.258
4.839ThrSer: 4.839 ± 0.417
6.092ThrThr: 6.092 ± 0.914
4.839ThrVal: 4.839 ± 0.55
0.713ThrTrp: 0.713 ± 0.124
2.526ThrTyr: 2.526 ± 0.23
0.0ThrXaa: 0.0 ± 0.0
Val
4.068ValAla: 4.068 ± 0.301
0.598ValCys: 0.598 ± 0.115
4.704ValAsp: 4.704 ± 0.298
4.473ValGlu: 4.473 ± 0.306
2.583ValPhe: 2.583 ± 0.262
5.822ValGly: 5.822 ± 0.717
0.868ValHis: 0.868 ± 0.125
3.605ValIle: 3.605 ± 0.297
3.624ValLys: 3.624 ± 0.209
4.415ValLeu: 4.415 ± 0.285
1.234ValMet: 1.234 ± 0.154
3.798ValAsn: 3.798 ± 0.321
2.487ValPro: 2.487 ± 0.178
2.198ValGln: 2.198 ± 0.205
2.641ValArg: 2.641 ± 0.212
5.726ValSer: 5.726 ± 0.511
5.398ValThr: 5.398 ± 0.487
4.955ValVal: 4.955 ± 0.353
0.424ValTrp: 0.424 ± 0.091
3.142ValTyr: 3.142 ± 0.28
0.0ValXaa: 0.0 ± 0.0
Trp
0.733TrpAla: 0.733 ± 0.115
0.212TrpCys: 0.212 ± 0.079
0.694TrpAsp: 0.694 ± 0.136
0.752TrpGlu: 0.752 ± 0.15
0.54TrpPhe: 0.54 ± 0.122
0.906TrpGly: 0.906 ± 0.155
0.289TrpHis: 0.289 ± 0.082
0.752TrpIle: 0.752 ± 0.154
0.733TrpLys: 0.733 ± 0.15
0.868TrpLeu: 0.868 ± 0.151
0.193TrpMet: 0.193 ± 0.059
0.752TrpAsn: 0.752 ± 0.125
0.251TrpPro: 0.251 ± 0.069
0.386TrpGln: 0.386 ± 0.103
0.424TrpArg: 0.424 ± 0.104
0.578TrpSer: 0.578 ± 0.127
0.964TrpThr: 0.964 ± 0.169
0.694TrpVal: 0.694 ± 0.128
0.174TrpTrp: 0.174 ± 0.066
0.443TrpTyr: 0.443 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.198TyrAla: 2.198 ± 0.186
0.54TyrCys: 0.54 ± 0.109
3.47TyrAsp: 3.47 ± 0.294
2.95TyrGlu: 2.95 ± 0.281
2.005TyrPhe: 2.005 ± 0.174
2.603TyrGly: 2.603 ± 0.208
0.829TyrHis: 0.829 ± 0.123
2.583TyrIle: 2.583 ± 0.231
2.256TyrLys: 2.256 ± 0.245
3.085TyrLeu: 3.085 ± 0.244
0.945TyrMet: 0.945 ± 0.161
2.93TyrAsn: 2.93 ± 0.201
1.427TyrPro: 1.427 ± 0.162
1.947TyrGln: 1.947 ± 0.243
1.947TyrArg: 1.947 ± 0.192
2.622TyrSer: 2.622 ± 0.236
2.757TyrThr: 2.757 ± 0.234
2.892TyrVal: 2.892 ± 0.26
0.424TyrTrp: 0.424 ± 0.109
2.159TyrTyr: 2.159 ± 0.239
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 202 proteins (51871 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski