Amino acid dipepetide frequency for Brenneria alni

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.979AlaAla: 9.979 ± 0.147	1.094AlaCys: 1.094 ± 0.036	5.188AlaAsp: 5.188 ± 0.085	5.77AlaGlu: 5.77 ± 0.085	3.412AlaPhe: 3.412 ± 0.054	7.434AlaGly: 7.434 ± 0.106	1.774AlaHis: 1.774 ± 0.043	5.896AlaIle: 5.896 ± 0.077	3.558AlaLys: 3.558 ± 0.073	11.845AlaLeu: 11.845 ± 0.113	2.664AlaMet: 2.664 ± 0.049	3.124AlaAsn: 3.124 ± 0.058	3.412AlaPro: 3.412 ± 0.065	4.605AlaGln: 4.605 ± 0.073	5.413AlaArg: 5.413 ± 0.074	5.576AlaSer: 5.576 ± 0.078	4.614AlaThr: 4.614 ± 0.074	6.641AlaVal: 6.641 ± 0.086	1.196AlaTrp: 1.196 ± 0.033	2.351AlaTyr: 2.351 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.916CysAla: 0.916 ± 0.031	0.179CysCys: 0.179 ± 0.012	0.59CysAsp: 0.59 ± 0.022	0.582CysGlu: 0.582 ± 0.025	0.403CysPhe: 0.403 ± 0.02	1.035CysGly: 1.035 ± 0.03	0.317CysHis: 0.317 ± 0.019	0.604CysIle: 0.604 ± 0.025	0.298CysLys: 0.298 ± 0.016	1.04CysLeu: 1.04 ± 0.03	0.236CysMet: 0.236 ± 0.014	0.339CysAsn: 0.339 ± 0.018	0.486CysPro: 0.486 ± 0.023	0.51CysGln: 0.51 ± 0.022	0.688CysArg: 0.688 ± 0.026	0.617CysSer: 0.617 ± 0.029	0.458CysThr: 0.458 ± 0.022	0.735CysVal: 0.735 ± 0.027	0.189CysTrp: 0.189 ± 0.015	0.351CysTyr: 0.351 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	5.098AspAla: 5.098 ± 0.066	0.518AspCys: 0.518 ± 0.019	3.059AspAsp: 3.059 ± 0.067	3.712AspGlu: 3.712 ± 0.062	2.14AspPhe: 2.14 ± 0.045	3.984AspGly: 3.984 ± 0.095	1.007AspHis: 1.007 ± 0.034	3.827AspIle: 3.827 ± 0.062	2.401AspLys: 2.401 ± 0.046	4.868AspLeu: 4.868 ± 0.078	1.319AspMet: 1.319 ± 0.037	2.237AspAsn: 2.237 ± 0.044	2.261AspPro: 2.261 ± 0.043	1.779AspGln: 1.779 ± 0.037	2.843AspArg: 2.843 ± 0.052	2.988AspSer: 2.988 ± 0.062	2.588AspThr: 2.588 ± 0.052	3.758AspVal: 3.758 ± 0.063	0.884AspTrp: 0.884 ± 0.029	1.931AspTyr: 1.931 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	4.94GluAla: 4.94 ± 0.084	0.486GluCys: 0.486 ± 0.021	2.338GluAsp: 2.338 ± 0.053	3.201GluGlu: 3.201 ± 0.067	1.824GluPhe: 1.824 ± 0.043	3.267GluGly: 3.267 ± 0.058	1.442GluHis: 1.442 ± 0.04	3.452GluIle: 3.452 ± 0.056	3.184GluLys: 3.184 ± 0.068	6.077GluLeu: 6.077 ± 0.081	1.731GluMet: 1.731 ± 0.046	2.443GluAsn: 2.443 ± 0.051	2.132GluPro: 2.132 ± 0.042	3.635GluGln: 3.635 ± 0.072	4.083GluArg: 4.083 ± 0.061	3.152GluSer: 3.152 ± 0.063	2.834GluThr: 2.834 ± 0.052	3.658GluVal: 3.658 ± 0.059	0.744GluTrp: 0.744 ± 0.028	1.45GluTyr: 1.45 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	3.364PheAla: 3.364 ± 0.058	0.479PheCys: 0.479 ± 0.02	2.305PheAsp: 2.305 ± 0.051	1.615PheGlu: 1.615 ± 0.039	1.603PhePhe: 1.603 ± 0.043	2.963PheGly: 2.963 ± 0.063	0.806PheHis: 0.806 ± 0.027	2.503PheIle: 2.503 ± 0.055	1.268PheLys: 1.268 ± 0.037	3.287PheLeu: 3.287 ± 0.064	0.886PheMet: 0.886 ± 0.026	1.689PheAsn: 1.689 ± 0.041	1.602PhePro: 1.602 ± 0.039	1.193PheGln: 1.193 ± 0.033	1.906PheArg: 1.906 ± 0.04	3.201PheSer: 3.201 ± 0.06	2.202PheThr: 2.202 ± 0.048	2.306PheVal: 2.306 ± 0.048	0.555PheTrp: 0.555 ± 0.023	1.169PheTyr: 1.169 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	6.044GlyAla: 6.044 ± 0.093	1.024GlyCys: 1.024 ± 0.032	3.723GlyAsp: 3.723 ± 0.06	4.465GlyGlu: 4.465 ± 0.075	3.02GlyPhe: 3.02 ± 0.053	5.3GlyGly: 5.3 ± 0.082	1.583GlyHis: 1.583 ± 0.04	5.014GlyIle: 5.014 ± 0.082	3.934GlyLys: 3.934 ± 0.06	7.382GlyLeu: 7.382 ± 0.078	2.185GlyMet: 2.185 ± 0.046	2.779GlyAsn: 2.779 ± 0.055	1.974GlyPro: 1.974 ± 0.047	3.056GlyGln: 3.056 ± 0.06	3.989GlyArg: 3.989 ± 0.069	4.268GlySer: 4.268 ± 0.078	3.533GlyThr: 3.533 ± 0.066	5.599GlyVal: 5.599 ± 0.088	1.195GlyTrp: 1.195 ± 0.034	2.565GlyTyr: 2.565 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	1.864HisAla: 1.864 ± 0.054	0.343HisCys: 0.343 ± 0.019	1.27HisAsp: 1.27 ± 0.036	1.084HisGlu: 1.084 ± 0.029	1.025HisPhe: 1.025 ± 0.029	1.634HisGly: 1.634 ± 0.044	0.825HisHis: 0.825 ± 0.031	1.377HisIle: 1.377 ± 0.033	0.802HisLys: 0.802 ± 0.027	2.265HisLeu: 2.265 ± 0.051	0.477HisMet: 0.477 ± 0.019	0.811HisAsn: 0.811 ± 0.025	1.338HisPro: 1.338 ± 0.034	1.372HisGln: 1.372 ± 0.041	1.324HisArg: 1.324 ± 0.041	1.264HisSer: 1.264 ± 0.033	1.048HisThr: 1.048 ± 0.031	1.264HisVal: 1.264 ± 0.035	0.394HisTrp: 0.394 ± 0.021	0.859HisTyr: 0.859 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.482IleAla: 6.482 ± 0.08	0.617IleCys: 0.617 ± 0.024	3.785IleAsp: 3.785 ± 0.057	3.673IleGlu: 3.673 ± 0.068	2.015IlePhe: 2.015 ± 0.055	4.837IleGly: 4.837 ± 0.077	1.23IleHis: 1.23 ± 0.032	3.52IleIle: 3.52 ± 0.073	2.492IleLys: 2.492 ± 0.05	5.197IleLeu: 5.197 ± 0.08	1.311IleMet: 1.311 ± 0.038	2.704IleAsn: 2.704 ± 0.053	2.773IlePro: 2.773 ± 0.042	2.137IleGln: 2.137 ± 0.046	3.358IleArg: 3.358 ± 0.053	4.157IleSer: 4.157 ± 0.074	3.664IleThr: 3.664 ± 0.063	4.025IleVal: 4.025 ± 0.054	0.67IleTrp: 0.67 ± 0.026	1.596IleTyr: 1.596 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	3.809LysAla: 3.809 ± 0.068	0.251LysCys: 0.251 ± 0.014	2.013LysAsp: 2.013 ± 0.051	2.238LysGlu: 2.238 ± 0.053	1.093LysPhe: 1.093 ± 0.031	2.726LysGly: 2.726 ± 0.052	0.903LysHis: 0.903 ± 0.026	2.512LysIle: 2.512 ± 0.052	2.188LysLys: 2.188 ± 0.062	4.26LysLeu: 4.26 ± 0.062	1.13LysMet: 1.13 ± 0.033	1.766LysAsn: 1.766 ± 0.042	2.065LysPro: 2.065 ± 0.046	2.151LysGln: 2.151 ± 0.043	2.524LysArg: 2.524 ± 0.05	2.44LysSer: 2.44 ± 0.054	2.596LysThr: 2.596 ± 0.048	2.78LysVal: 2.78 ± 0.045	0.391LysTrp: 0.391 ± 0.02	1.09LysTyr: 1.09 ± 0.032	0.0LysXaa: 0.0 ± 0.0
Leu	11.558LeuAla: 11.558 ± 0.113	1.252LeuCys: 1.252 ± 0.035	5.767LeuAsp: 5.767 ± 0.076	5.402LeuGlu: 5.402 ± 0.081	4.163LeuPhe: 4.163 ± 0.063	7.345LeuGly: 7.345 ± 0.091	2.353LeuHis: 2.353 ± 0.052	6.209LeuIle: 6.209 ± 0.082	4.461LeuLys: 4.461 ± 0.072	12.442LeuLeu: 12.442 ± 0.157	2.879LeuMet: 2.879 ± 0.053	4.42LeuAsn: 4.42 ± 0.061	5.813LeuPro: 5.813 ± 0.081	4.527LeuGln: 4.527 ± 0.075	6.56LeuArg: 6.56 ± 0.091	7.797LeuSer: 7.797 ± 0.091	6.44LeuThr: 6.44 ± 0.099	6.937LeuVal: 6.937 ± 0.093	1.266LeuTrp: 1.266 ± 0.033	2.693LeuTyr: 2.693 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.691MetAla: 2.691 ± 0.047	0.174MetCys: 0.174 ± 0.012	1.192MetAsp: 1.192 ± 0.032	1.27MetGlu: 1.27 ± 0.039	0.794MetPhe: 0.794 ± 0.024	1.716MetGly: 1.716 ± 0.036	0.477MetHis: 0.477 ± 0.02	1.428MetIle: 1.428 ± 0.038	1.264MetLys: 1.264 ± 0.034	3.135MetLeu: 3.135 ± 0.053	0.82MetMet: 0.82 ± 0.028	1.037MetAsn: 1.037 ± 0.03	1.288MetPro: 1.288 ± 0.035	1.186MetGln: 1.186 ± 0.031	1.494MetArg: 1.494 ± 0.038	1.815MetSer: 1.815 ± 0.039	1.641MetThr: 1.641 ± 0.039	1.827MetVal: 1.827 ± 0.038	0.19MetTrp: 0.19 ± 0.013	0.497MetTyr: 0.497 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.575AsnAla: 3.575 ± 0.059	0.338AsnCys: 0.338 ± 0.018	2.078AsnAsp: 2.078 ± 0.048	2.011AsnGlu: 2.011 ± 0.039	1.217AsnPhe: 1.217 ± 0.037	2.916AsnGly: 2.916 ± 0.058	0.893AsnHis: 0.893 ± 0.033	2.463AsnIle: 2.463 ± 0.051	1.589AsnLys: 1.589 ± 0.038	3.65AsnLeu: 3.65 ± 0.057	0.948AsnMet: 0.948 ± 0.031	1.661AsnAsn: 1.661 ± 0.053	2.124AsnPro: 2.124 ± 0.046	1.731AsnGln: 1.731 ± 0.046	2.171AsnArg: 2.171 ± 0.046	2.137AsnSer: 2.137 ± 0.049	2.111AsnThr: 2.111 ± 0.052	2.495AsnVal: 2.495 ± 0.043	0.512AsnTrp: 0.512 ± 0.022	1.131AsnTyr: 1.131 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	4.422ProAla: 4.422 ± 0.074	0.389ProCys: 0.389 ± 0.019	2.838ProAsp: 2.838 ± 0.055	3.204ProGlu: 3.204 ± 0.05	1.745ProPhe: 1.745 ± 0.04	3.263ProGly: 3.263 ± 0.061	1.062ProHis: 1.062 ± 0.03	2.124ProIle: 2.124 ± 0.044	1.471ProLys: 1.471 ± 0.04	5.258ProLeu: 5.258 ± 0.077	1.033ProMet: 1.033 ± 0.029	1.309ProAsn: 1.309 ± 0.034	1.768ProPro: 1.768 ± 0.045	2.272ProGln: 2.272 ± 0.044	1.983ProArg: 1.983 ± 0.039	2.397ProSer: 2.397 ± 0.047	2.247ProThr: 2.247 ± 0.043	3.694ProVal: 3.694 ± 0.058	0.656ProTrp: 0.656 ± 0.023	1.385ProTyr: 1.385 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	5.156GlnAla: 5.156 ± 0.084	0.391GlnCys: 0.391 ± 0.019	2.098GlnAsp: 2.098 ± 0.044	2.321GlnGlu: 2.321 ± 0.052	1.446GlnPhe: 1.446 ± 0.038	3.224GlnGly: 3.224 ± 0.056	1.333GlnHis: 1.333 ± 0.037	2.587GlnIle: 2.587 ± 0.049	1.796GlnLys: 1.796 ± 0.037	5.378GlnLeu: 5.378 ± 0.092	1.164GlnMet: 1.164 ± 0.032	1.481GlnAsn: 1.481 ± 0.038	2.396GlnPro: 2.396 ± 0.049	3.989GlnGln: 3.989 ± 0.096	3.61GlnArg: 3.61 ± 0.069	2.6GlnSer: 2.6 ± 0.047	2.335GlnThr: 2.335 ± 0.046	3.127GlnVal: 3.127 ± 0.054	0.678GlnTrp: 0.678 ± 0.025	1.195GlnTyr: 1.195 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	4.605ArgAla: 4.605 ± 0.07	0.634ArgCys: 0.634 ± 0.027	3.161ArgAsp: 3.161 ± 0.055	3.708ArgGlu: 3.708 ± 0.072	2.575ArgPhe: 2.575 ± 0.048	3.418ArgGly: 3.418 ± 0.055	1.692ArgHis: 1.692 ± 0.042	3.785ArgIle: 3.785 ± 0.064	2.369ArgLys: 2.369 ± 0.051	7.058ArgLeu: 7.058 ± 0.076	1.539ArgMet: 1.539 ± 0.039	2.116ArgAsn: 2.116 ± 0.042	2.26ArgPro: 2.26 ± 0.042	3.811ArgGln: 3.811 ± 0.07	3.913ArgArg: 3.913 ± 0.069	3.013ArgSer: 3.013 ± 0.058	2.616ArgThr: 2.616 ± 0.046	3.816ArgVal: 3.816 ± 0.063	1.007ArgTrp: 1.007 ± 0.039	2.231ArgTyr: 2.231 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	6.013SerAla: 6.013 ± 0.075	0.609SerCys: 0.609 ± 0.023	3.298SerAsp: 3.298 ± 0.068	3.395SerGlu: 3.395 ± 0.05	2.307SerPhe: 2.307 ± 0.043	5.576SerGly: 5.576 ± 0.096	1.405SerHis: 1.405 ± 0.036	3.288SerIle: 3.288 ± 0.061	2.017SerLys: 2.017 ± 0.04	7.126SerLeu: 7.126 ± 0.087	1.548SerMet: 1.548 ± 0.038	1.985SerAsn: 1.985 ± 0.043	2.744SerPro: 2.744 ± 0.047	2.811SerGln: 2.811 ± 0.047	3.593SerArg: 3.593 ± 0.063	3.93SerSer: 3.93 ± 0.075	3.091SerThr: 3.091 ± 0.06	4.36SerVal: 4.36 ± 0.074	0.915SerTrp: 0.915 ± 0.033	1.624SerTyr: 1.624 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	5.011ThrAla: 5.011 ± 0.072	0.45ThrCys: 0.45 ± 0.021	2.79ThrAsp: 2.79 ± 0.051	2.803ThrGlu: 2.803 ± 0.05	1.858ThrPhe: 1.858 ± 0.039	4.245ThrGly: 4.245 ± 0.074	1.204ThrHis: 1.204 ± 0.033	2.9ThrIle: 2.9 ± 0.057	1.472ThrLys: 1.472 ± 0.036	7.359ThrLeu: 7.359 ± 0.103	1.068ThrMet: 1.068 ± 0.031	1.618ThrAsn: 1.618 ± 0.038	3.133ThrPro: 3.133 ± 0.051	2.248ThrGln: 2.248 ± 0.054	2.971ThrArg: 2.971 ± 0.046	2.994ThrSer: 2.994 ± 0.054	2.831ThrThr: 2.831 ± 0.062	3.881ThrVal: 3.881 ± 0.071	0.613ThrTrp: 0.613 ± 0.026	1.26ThrTyr: 1.26 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	6.632ValAla: 6.632 ± 0.078	0.77ValCys: 0.77 ± 0.026	3.647ValAsp: 3.647 ± 0.062	3.878ValGlu: 3.878 ± 0.065	2.511ValPhe: 2.511 ± 0.045	4.721ValGly: 4.721 ± 0.078	1.168ValHis: 1.168 ± 0.032	4.632ValIle: 4.632 ± 0.078	2.995ValLys: 2.995 ± 0.056	7.223ValLeu: 7.223 ± 0.092	2.073ValMet: 2.073 ± 0.045	2.792ValAsn: 2.792 ± 0.062	2.828ValPro: 2.828 ± 0.046	2.364ValGln: 2.364 ± 0.046	3.795ValArg: 3.795 ± 0.058	4.744ValSer: 4.744 ± 0.072	4.017ValThr: 4.017 ± 0.069	5.237ValVal: 5.237 ± 0.083	0.892ValTrp: 0.892 ± 0.031	1.758ValTyr: 1.758 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.92TrpAla: 0.92 ± 0.035	0.168TrpCys: 0.168 ± 0.013	0.58TrpAsp: 0.58 ± 0.026	0.556TrpGlu: 0.556 ± 0.022	0.579TrpPhe: 0.579 ± 0.024	0.85TrpGly: 0.85 ± 0.025	0.38TrpHis: 0.38 ± 0.019	0.71TrpIle: 0.71 ± 0.024	0.443TrpLys: 0.443 ± 0.021	2.214TrpLeu: 2.214 ± 0.049	0.348TrpMet: 0.348 ± 0.017	0.436TrpAsn: 0.436 ± 0.018	0.639TrpPro: 0.639 ± 0.029	1.137TrpGln: 1.137 ± 0.039	1.042TrpArg: 1.042 ± 0.031	0.769TrpSer: 0.769 ± 0.026	0.441TrpThr: 0.441 ± 0.02	0.841TrpVal: 0.841 ± 0.028	0.23TrpTrp: 0.23 ± 0.013	0.376TrpTyr: 0.376 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.469TyrAla: 2.469 ± 0.045	0.405TyrCys: 0.405 ± 0.018	1.481TyrAsp: 1.481 ± 0.039	1.187TyrGlu: 1.187 ± 0.033	1.17TyrPhe: 1.17 ± 0.033	2.18TyrGly: 2.18 ± 0.048	0.787TyrHis: 0.787 ± 0.029	1.412TyrIle: 1.412 ± 0.039	0.925TyrLys: 0.925 ± 0.029	3.221TyrLeu: 3.221 ± 0.054	0.577TyrMet: 0.577 ± 0.024	1.004TyrAsn: 1.004 ± 0.035	1.483TyrPro: 1.483 ± 0.041	1.79TyrGln: 1.79 ± 0.041	2.07TyrArg: 2.07 ± 0.041	1.777TyrSer: 1.777 ± 0.041	1.4TyrThr: 1.4 ± 0.036	1.695TyrVal: 1.695 ± 0.039	0.48TyrTrp: 0.48 ± 0.022	0.944TyrTyr: 0.944 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3626 proteins (1149954 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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