Opitutae bacterium SCGC AG-212-L18

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; unclassified Opitutae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 203 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177QVD0|A0A177QVD0_9BACT Uncharacterized protein OS=Opitutae bacterium SCGC AG-212-L18 OX=1799660 GN=AYO37_00015 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.15KK3 pKa = 9.81QRR5 pKa = 11.84LTIGLLIIFLLGQIDD20 pKa = 3.77VWGQRR25 pKa = 11.84HH26 pKa = 5.68EE27 pKa = 4.9GKK29 pKa = 10.39FPPVFKK35 pKa = 11.2LEE37 pKa = 5.27DD38 pKa = 3.38INGTNGYY45 pKa = 9.32IIQGLHH51 pKa = 7.13DD52 pKa = 4.87FSQQINGKK60 pKa = 10.18GDD62 pKa = 3.38VNGDD66 pKa = 3.49GVKK69 pKa = 10.54DD70 pKa = 3.66VLIGNLFLEE79 pKa = 6.06GYY81 pKa = 7.16SQRR84 pKa = 11.84DD85 pKa = 2.96QVYY88 pKa = 9.68IIFGNKK94 pKa = 7.17EE95 pKa = 3.61KK96 pKa = 10.57RR97 pKa = 11.84PQVVDD102 pKa = 3.76PLHH105 pKa = 6.69INSSNGVIVNPGMLDD120 pKa = 3.18SFGLYY125 pKa = 10.1VSVIGDD131 pKa = 3.56INKK134 pKa = 10.51DD135 pKa = 3.64GIDD138 pKa = 4.37DD139 pKa = 3.73MLISNEE145 pKa = 3.96YY146 pKa = 9.61QDD148 pKa = 5.43NFLCVLFGRR157 pKa = 11.84KK158 pKa = 8.25HH159 pKa = 5.38WSTPIVITDD168 pKa = 4.32LMDD171 pKa = 3.85GKK173 pKa = 10.55NGFFIEE179 pKa = 4.64GDD181 pKa = 3.56SNLYY185 pKa = 7.85VTAISPAGDD194 pKa = 3.33INGDD198 pKa = 3.93GIDD201 pKa = 5.04DD202 pKa = 3.53ILMGVGGNNNIQNAGQSYY220 pKa = 8.36VVFGSKK226 pKa = 10.56GSWPAVINLKK236 pKa = 10.39DD237 pKa = 3.37INGNNGFIINGIYY250 pKa = 10.61AKK252 pKa = 10.46DD253 pKa = 3.39EE254 pKa = 4.17SGYY257 pKa = 10.01SVSGAGDD264 pKa = 3.59FNGDD268 pKa = 3.87GISDD272 pKa = 4.13VIIGAPFANNGQGQSYY288 pKa = 10.29IVFGSKK294 pKa = 10.69GPWPATIDD302 pKa = 3.77LGNLDD307 pKa = 4.35GSNGLTIYY315 pKa = 10.8GIDD318 pKa = 3.58SSDD321 pKa = 3.28LTGYY325 pKa = 9.78SVSGAGDD332 pKa = 3.33INGDD336 pKa = 3.94DD337 pKa = 4.06FDD339 pKa = 5.38DD340 pKa = 5.83VIIASNNNDD349 pKa = 2.67IYY351 pKa = 11.74VVFGNNATWPPLIDD365 pKa = 4.54LSNLNGKK372 pKa = 9.28NGFVLRR378 pKa = 11.84GLYY381 pKa = 9.93KK382 pKa = 10.47RR383 pKa = 11.84GPFSVSGAGDD393 pKa = 3.77FNADD397 pKa = 3.95NITDD401 pKa = 4.59IIIGTTDD408 pKa = 2.77AYY410 pKa = 10.03IDD412 pKa = 3.16EE413 pKa = 5.31GYY415 pKa = 10.16FRR417 pKa = 11.84RR418 pKa = 11.84QGYY421 pKa = 7.85VVFGNKK427 pKa = 9.45NMWPAEE433 pKa = 3.91INLNGLNGTGLNGTNGFIINGSFNNMHH460 pKa = 5.48VRR462 pKa = 11.84YY463 pKa = 9.29VVDD466 pKa = 5.5GIGDD470 pKa = 3.72VNGDD474 pKa = 3.79SVDD477 pKa = 4.71DD478 pKa = 3.85ILIGEE483 pKa = 4.52SYY485 pKa = 10.56GANPHH490 pKa = 6.25NGVPRR495 pKa = 11.84NGYY498 pKa = 8.76VLFGCCGEE506 pKa = 4.44VPPPPLSPIEE516 pKa = 4.18PLHH519 pKa = 6.45NSLALGLGIGFGAVGLITAGVVYY542 pKa = 8.7YY543 pKa = 9.12WWHH546 pKa = 6.13YY547 pKa = 10.01DD548 pKa = 3.57DD549 pKa = 5.3PVIGADD555 pKa = 4.97DD556 pKa = 3.61IAEE559 pKa = 4.31GPINVDD565 pKa = 3.15PEE567 pKa = 4.26KK568 pKa = 10.45TLLIPIDD575 pKa = 4.32APPSGVGWMTSSS587 pKa = 3.24

Molecular weight: 62.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177QTW9|A0A177QTW9_9BACT Uncharacterized protein OS=Opitutae bacterium SCGC AG-212-L18 OX=1799660 GN=AYO37_00955 PE=4 SV=1
MM1 pKa = 7.51NIKK4 pKa = 8.76TLLVHH9 pKa = 6.39PAWLFYY15 pKa = 10.13WKK17 pKa = 9.83SQKK20 pKa = 9.89RR21 pKa = 11.84RR22 pKa = 11.84GSFLFLGRR30 pKa = 11.84MRR32 pKa = 11.84AKK34 pKa = 10.31INLRR38 pKa = 11.84LFGRR42 pKa = 11.84TFLGFDD48 pKa = 3.37EE49 pKa = 4.98YY50 pKa = 11.45GVKK53 pKa = 10.13INLRR57 pKa = 11.84KK58 pKa = 9.54EE59 pKa = 3.63WLLSYY64 pKa = 10.85NLLVIKK70 pKa = 9.0EE71 pKa = 4.12LKK73 pKa = 9.56IDD75 pKa = 3.68LVINLFF81 pKa = 3.71

Molecular weight: 9.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

203

0

203

64958

60

1319

320.0

36.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.909 ± 0.263

1.267 ± 0.059

5.133 ± 0.117

7.219 ± 0.172

4.948 ± 0.129

5.767 ± 0.189

2.004 ± 0.076

8.164 ± 0.168

7.708 ± 0.189

10.444 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.082

5.348 ± 0.151

3.833 ± 0.127

3.687 ± 0.092

4.09 ± 0.128

6.626 ± 0.113

4.566 ± 0.11

5.427 ± 0.159

1.111 ± 0.061

3.471 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski