Amino acid dipepetide frequency for Opitutae bacterium SCGC AG-212-L18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.511AlaAla: 5.511 ± 0.599
1.031AlaCys: 1.031 ± 0.141
3.171AlaAsp: 3.171 ± 0.275
4.88AlaGlu: 4.88 ± 0.41
3.541AlaPhe: 3.541 ± 0.226
3.879AlaGly: 3.879 ± 0.317
1.693AlaHis: 1.693 ± 0.146
6.296AlaIle: 6.296 ± 0.351
5.265AlaLys: 5.265 ± 0.485
8.39AlaLeu: 8.39 ± 0.362
1.57AlaMet: 1.57 ± 0.176
2.786AlaAsn: 2.786 ± 0.24
2.186AlaPro: 2.186 ± 0.179
2.894AlaGln: 2.894 ± 0.346
2.54AlaArg: 2.54 ± 0.214
4.095AlaSer: 4.095 ± 0.28
2.848AlaThr: 2.848 ± 0.268
3.402AlaVal: 3.402 ± 0.299
0.831AlaTrp: 0.831 ± 0.105
2.278AlaTyr: 2.278 ± 0.195
0.0AlaXaa: 0.0 ± 0.0
Cys
0.878CysAla: 0.878 ± 0.121
0.246CysCys: 0.246 ± 0.065
0.739CysAsp: 0.739 ± 0.115
0.631CysGlu: 0.631 ± 0.102
0.801CysPhe: 0.801 ± 0.101
0.939CysGly: 0.939 ± 0.109
0.369CysHis: 0.369 ± 0.085
0.785CysIle: 0.785 ± 0.139
0.908CysLys: 0.908 ± 0.114
1.447CysLeu: 1.447 ± 0.183
0.277CysMet: 0.277 ± 0.067
0.554CysAsn: 0.554 ± 0.096
0.539CysPro: 0.539 ± 0.09
0.493CysGln: 0.493 ± 0.092
0.523CysArg: 0.523 ± 0.11
0.693CysSer: 0.693 ± 0.106
0.477CysThr: 0.477 ± 0.096
0.677CysVal: 0.677 ± 0.109
0.108CysTrp: 0.108 ± 0.042
0.585CysTyr: 0.585 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
3.418AspAla: 3.418 ± 0.324
0.616AspCys: 0.616 ± 0.086
2.386AspAsp: 2.386 ± 0.212
3.941AspGlu: 3.941 ± 0.273
3.218AspPhe: 3.218 ± 0.224
2.617AspGly: 2.617 ± 0.242
1.047AspHis: 1.047 ± 0.123
3.91AspIle: 3.91 ± 0.289
3.448AspLys: 3.448 ± 0.246
5.788AspLeu: 5.788 ± 0.304
1.001AspMet: 1.001 ± 0.122
2.155AspAsn: 2.155 ± 0.179
2.448AspPro: 2.448 ± 0.229
1.77AspGln: 1.77 ± 0.2
2.186AspArg: 2.186 ± 0.175
3.279AspSer: 3.279 ± 0.226
2.263AspThr: 2.263 ± 0.179
3.017AspVal: 3.017 ± 0.243
0.677AspTrp: 0.677 ± 0.115
2.14AspTyr: 2.14 ± 0.171
0.0AspXaa: 0.0 ± 0.0
Glu
6.312GluAla: 6.312 ± 0.556
0.878GluCys: 0.878 ± 0.123
3.51GluAsp: 3.51 ± 0.232
6.42GluGlu: 6.42 ± 0.398
2.586GluPhe: 2.586 ± 0.254
4.08GluGly: 4.08 ± 0.222
1.185GluHis: 1.185 ± 0.143
5.788GluIle: 5.788 ± 0.342
6.635GluLys: 6.635 ± 0.431
7.066GluLeu: 7.066 ± 0.37
1.786GluMet: 1.786 ± 0.165
4.11GluAsn: 4.11 ± 0.248
1.801GluPro: 1.801 ± 0.162
2.663GluGln: 2.663 ± 0.245
3.387GluArg: 3.387 ± 0.246
4.203GluSer: 4.203 ± 0.253
2.633GluThr: 2.633 ± 0.226
4.326GluVal: 4.326 ± 0.302
0.585GluTrp: 0.585 ± 0.089
2.232GluTyr: 2.232 ± 0.207
0.0GluXaa: 0.0 ± 0.0
Phe
2.586PheAla: 2.586 ± 0.21
0.816PheCys: 0.816 ± 0.125
2.556PheAsp: 2.556 ± 0.199
3.402PheGlu: 3.402 ± 0.239
2.833PhePhe: 2.833 ± 0.248
3.094PheGly: 3.094 ± 0.267
0.97PheHis: 0.97 ± 0.116
3.679PheIle: 3.679 ± 0.306
3.71PheLys: 3.71 ± 0.266
5.727PheLeu: 5.727 ± 0.383
0.939PheMet: 0.939 ± 0.127
2.74PheAsn: 2.74 ± 0.198
1.786PhePro: 1.786 ± 0.179
1.57PheGln: 1.57 ± 0.16
1.817PheArg: 1.817 ± 0.168
4.495PheSer: 4.495 ± 0.298
2.063PheThr: 2.063 ± 0.161
2.294PheVal: 2.294 ± 0.221
0.693PheTrp: 0.693 ± 0.101
1.709PheTyr: 1.709 ± 0.193
0.0PheXaa: 0.0 ± 0.0
Gly
4.249GlyAla: 4.249 ± 0.354
0.616GlyCys: 0.616 ± 0.095
2.894GlyAsp: 2.894 ± 0.296
3.125GlyGlu: 3.125 ± 0.227
3.094GlyPhe: 3.094 ± 0.245
3.448GlyGly: 3.448 ± 0.263
1.108GlyHis: 1.108 ± 0.119
5.126GlyIle: 5.126 ± 0.324
3.895GlyLys: 3.895 ± 0.284
6.527GlyLeu: 6.527 ± 0.327
1.293GlyMet: 1.293 ± 0.158
2.371GlyAsn: 2.371 ± 0.187
2.032GlyPro: 2.032 ± 0.347
2.124GlyGln: 2.124 ± 0.206
2.494GlyArg: 2.494 ± 0.21
3.618GlySer: 3.618 ± 0.257
2.648GlyThr: 2.648 ± 0.18
3.972GlyVal: 3.972 ± 0.291
0.585GlyTrp: 0.585 ± 0.099
2.448GlyTyr: 2.448 ± 0.213
0.0GlyXaa: 0.0 ± 0.0
His
1.216HisAla: 1.216 ± 0.152
0.262HisCys: 0.262 ± 0.075
0.893HisAsp: 0.893 ± 0.11
1.355HisGlu: 1.355 ± 0.169
1.17HisPhe: 1.17 ± 0.135
0.939HisGly: 0.939 ± 0.111
0.493HisHis: 0.493 ± 0.095
1.447HisIle: 1.447 ± 0.168
1.047HisLys: 1.047 ± 0.139
2.263HisLeu: 2.263 ± 0.229
0.354HisMet: 0.354 ± 0.066
0.954HisAsn: 0.954 ± 0.141
1.293HisPro: 1.293 ± 0.146
0.847HisGln: 0.847 ± 0.107
0.97HisArg: 0.97 ± 0.122
1.324HisSer: 1.324 ± 0.172
0.847HisThr: 0.847 ± 0.113
1.078HisVal: 1.078 ± 0.125
0.323HisTrp: 0.323 ± 0.093
0.97HisTyr: 0.97 ± 0.126
0.0HisXaa: 0.0 ± 0.0
Ile
5.619IleAla: 5.619 ± 0.353
0.954IleCys: 0.954 ± 0.117
5.173IleAsp: 5.173 ± 0.301
6.112IleGlu: 6.112 ± 0.318
4.141IlePhe: 4.141 ± 0.307
4.357IleGly: 4.357 ± 0.297
1.57IleHis: 1.57 ± 0.186
5.896IleIle: 5.896 ± 0.339
6.635IleLys: 6.635 ± 0.382
7.944IleLeu: 7.944 ± 0.401
1.601IleMet: 1.601 ± 0.18
4.896IleAsn: 4.896 ± 0.34
3.541IlePro: 3.541 ± 0.243
3.064IleGln: 3.064 ± 0.212
3.033IleArg: 3.033 ± 0.23
5.558IleSer: 5.558 ± 0.309
3.91IleThr: 3.91 ± 0.2
4.326IleVal: 4.326 ± 0.301
0.862IleTrp: 0.862 ± 0.103
2.448IleTyr: 2.448 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
5.388LysAla: 5.388 ± 0.332
0.631LysCys: 0.631 ± 0.107
4.326LysAsp: 4.326 ± 0.254
6.989LysGlu: 6.989 ± 0.418
2.663LysPhe: 2.663 ± 0.239
4.033LysGly: 4.033 ± 0.241
1.37LysHis: 1.37 ± 0.146
6.897LysIle: 6.897 ± 0.381
7.066LysLys: 7.066 ± 0.439
6.866LysLeu: 6.866 ± 0.304
1.894LysMet: 1.894 ± 0.191
5.296LysAsn: 5.296 ± 0.351
2.201LysPro: 2.201 ± 0.226
2.956LysGln: 2.956 ± 0.245
3.079LysArg: 3.079 ± 0.21
4.464LysSer: 4.464 ± 0.29
4.203LysThr: 4.203 ± 0.311
3.818LysVal: 3.818 ± 0.267
0.739LysTrp: 0.739 ± 0.118
2.201LysTyr: 2.201 ± 0.189
0.0LysXaa: 0.0 ± 0.0
Leu
6.974LeuAla: 6.974 ± 0.471
1.37LeuCys: 1.37 ± 0.179
4.896LeuAsp: 4.896 ± 0.285
7.559LeuGlu: 7.559 ± 0.428
5.019LeuPhe: 5.019 ± 0.389
6.481LeuGly: 6.481 ± 0.42
1.863LeuHis: 1.863 ± 0.175
8.467LeuIle: 8.467 ± 0.373
8.914LeuLys: 8.914 ± 0.452
10.268LeuLeu: 10.268 ± 0.527
2.34LeuMet: 2.34 ± 0.195
6.096LeuAsn: 6.096 ± 0.296
4.018LeuPro: 4.018 ± 0.275
3.264LeuGln: 3.264 ± 0.216
4.449LeuArg: 4.449 ± 0.287
8.175LeuSer: 8.175 ± 0.359
4.911LeuThr: 4.911 ± 0.278
5.173LeuVal: 5.173 ± 0.254
1.016LeuTrp: 1.016 ± 0.154
3.187LeuTyr: 3.187 ± 0.25
0.0LeuXaa: 0.0 ± 0.0
Met
1.663MetAla: 1.663 ± 0.18
0.185MetCys: 0.185 ± 0.062
1.401MetAsp: 1.401 ± 0.141
1.678MetGlu: 1.678 ± 0.16
0.862MetPhe: 0.862 ± 0.116
1.478MetGly: 1.478 ± 0.16
0.446MetHis: 0.446 ± 0.082
1.586MetIle: 1.586 ± 0.149
2.078MetLys: 2.078 ± 0.159
2.309MetLeu: 2.309 ± 0.206
0.539MetMet: 0.539 ± 0.101
1.463MetAsn: 1.463 ± 0.179
1.047MetPro: 1.047 ± 0.104
0.893MetGln: 0.893 ± 0.114
1.124MetArg: 1.124 ± 0.165
1.185MetSer: 1.185 ± 0.148
0.985MetThr: 0.985 ± 0.125
1.262MetVal: 1.262 ± 0.152
0.092MetTrp: 0.092 ± 0.038
0.508MetTyr: 0.508 ± 0.081
0.0MetXaa: 0.0 ± 0.0
Asn
3.587AsnAla: 3.587 ± 0.275
0.77AsnCys: 0.77 ± 0.111
2.91AsnAsp: 2.91 ± 0.24
3.618AsnGlu: 3.618 ± 0.239
2.309AsnPhe: 2.309 ± 0.2
2.448AsnGly: 2.448 ± 0.349
1.155AsnHis: 1.155 ± 0.142
4.464AsnIle: 4.464 ± 0.297
4.557AsnLys: 4.557 ± 0.276
5.357AsnLeu: 5.357 ± 0.307
1.139AsnMet: 1.139 ± 0.118
3.279AsnAsn: 3.279 ± 0.337
2.633AsnPro: 2.633 ± 0.23
2.094AsnGln: 2.094 ± 0.162
2.14AsnArg: 2.14 ± 0.187
3.371AsnSer: 3.371 ± 0.251
2.371AsnThr: 2.371 ± 0.206
2.355AsnVal: 2.355 ± 0.197
0.724AsnTrp: 0.724 ± 0.109
2.201AsnTyr: 2.201 ± 0.196
0.0AsnXaa: 0.0 ± 0.0
Pro
2.155ProAla: 2.155 ± 0.232
0.431ProCys: 0.431 ± 0.096
2.017ProAsp: 2.017 ± 0.195
3.233ProGlu: 3.233 ± 0.251
1.986ProPhe: 1.986 ± 0.215
2.355ProGly: 2.355 ± 0.217
0.878ProHis: 0.878 ± 0.119
3.11ProIle: 3.11 ± 0.195
3.002ProLys: 3.002 ± 0.225
3.864ProLeu: 3.864 ± 0.271
0.754ProMet: 0.754 ± 0.119
2.124ProAsn: 2.124 ± 0.192
1.262ProPro: 1.262 ± 0.162
1.262ProGln: 1.262 ± 0.135
1.509ProArg: 1.509 ± 0.151
2.556ProSer: 2.556 ± 0.251
1.678ProThr: 1.678 ± 0.232
2.155ProVal: 2.155 ± 0.186
0.493ProTrp: 0.493 ± 0.093
1.509ProTyr: 1.509 ± 0.176
0.0ProXaa: 0.0 ± 0.0
Gln
2.694GlnAla: 2.694 ± 0.302
0.416GlnCys: 0.416 ± 0.078
1.555GlnAsp: 1.555 ± 0.189
3.017GlnGlu: 3.017 ± 0.239
1.847GlnPhe: 1.847 ± 0.175
2.171GlnGly: 2.171 ± 0.221
0.693GlnHis: 0.693 ± 0.104
2.725GlnIle: 2.725 ± 0.21
3.341GlnLys: 3.341 ± 0.327
3.864GlnLeu: 3.864 ± 0.273
0.831GlnMet: 0.831 ± 0.109
2.155GlnAsn: 2.155 ± 0.163
1.031GlnPro: 1.031 ± 0.127
1.262GlnGln: 1.262 ± 0.135
1.386GlnArg: 1.386 ± 0.138
2.263GlnSer: 2.263 ± 0.199
1.74GlnThr: 1.74 ± 0.176
2.078GlnVal: 2.078 ± 0.172
0.4GlnTrp: 0.4 ± 0.084
1.401GlnTyr: 1.401 ± 0.152
0.0GlnXaa: 0.0 ± 0.0
Arg
3.064ArgAla: 3.064 ± 0.267
0.647ArgCys: 0.647 ± 0.087
2.048ArgAsp: 2.048 ± 0.195
3.064ArgGlu: 3.064 ± 0.223
2.186ArgPhe: 2.186 ± 0.179
2.448ArgGly: 2.448 ± 0.225
0.724ArgHis: 0.724 ± 0.1
3.156ArgIle: 3.156 ± 0.214
2.725ArgLys: 2.725 ± 0.216
4.311ArgLeu: 4.311 ± 0.283
1.078ArgMet: 1.078 ± 0.119
1.786ArgAsn: 1.786 ± 0.159
1.416ArgPro: 1.416 ± 0.166
1.724ArgGln: 1.724 ± 0.138
1.817ArgArg: 1.817 ± 0.173
2.879ArgSer: 2.879 ± 0.215
1.447ArgThr: 1.447 ± 0.163
2.586ArgVal: 2.586 ± 0.22
0.57ArgTrp: 0.57 ± 0.104
1.216ArgTyr: 1.216 ± 0.128
0.0ArgXaa: 0.0 ± 0.0
Ser
3.664SerAla: 3.664 ± 0.226
0.693SerCys: 0.693 ± 0.107
2.925SerAsp: 2.925 ± 0.214
4.049SerGlu: 4.049 ± 0.21
3.833SerPhe: 3.833 ± 0.324
4.234SerGly: 4.234 ± 0.253
1.293SerHis: 1.293 ± 0.18
6.127SerIle: 6.127 ± 0.3
5.111SerLys: 5.111 ± 0.282
7.066SerLeu: 7.066 ± 0.308
1.863SerMet: 1.863 ± 0.189
3.402SerAsn: 3.402 ± 0.208
2.525SerPro: 2.525 ± 0.221
2.248SerGln: 2.248 ± 0.179
2.602SerArg: 2.602 ± 0.2
4.803SerSer: 4.803 ± 0.391
2.971SerThr: 2.971 ± 0.225
3.387SerVal: 3.387 ± 0.225
0.816SerTrp: 0.816 ± 0.119
2.648SerTyr: 2.648 ± 0.202
0.0SerXaa: 0.0 ± 0.0
Thr
3.371ThrAla: 3.371 ± 0.217
0.57ThrCys: 0.57 ± 0.1
2.201ThrAsp: 2.201 ± 0.231
2.54ThrGlu: 2.54 ± 0.161
1.909ThrPhe: 1.909 ± 0.199
2.771ThrGly: 2.771 ± 0.284
1.232ThrHis: 1.232 ± 0.152
4.018ThrIle: 4.018 ± 0.296
2.571ThrLys: 2.571 ± 0.209
4.973ThrLeu: 4.973 ± 0.289
1.093ThrMet: 1.093 ± 0.137
2.263ThrAsn: 2.263 ± 0.211
2.309ThrPro: 2.309 ± 0.237
2.017ThrGln: 2.017 ± 0.181
1.786ThrArg: 1.786 ± 0.145
2.494ThrSer: 2.494 ± 0.199
2.817ThrThr: 2.817 ± 0.258
2.648ThrVal: 2.648 ± 0.178
0.508ThrTrp: 0.508 ± 0.082
1.57ThrTyr: 1.57 ± 0.145
0.0ThrXaa: 0.0 ± 0.0
Val
3.756ValAla: 3.756 ± 0.243
0.831ValCys: 0.831 ± 0.114
3.002ValAsp: 3.002 ± 0.222
3.664ValGlu: 3.664 ± 0.259
2.802ValPhe: 2.802 ± 0.208
3.279ValGly: 3.279 ± 0.236
1.124ValHis: 1.124 ± 0.15
4.372ValIle: 4.372 ± 0.294
3.464ValLys: 3.464 ± 0.245
6.035ValLeu: 6.035 ± 0.288
1.232ValMet: 1.232 ± 0.132
2.54ValAsn: 2.54 ± 0.204
2.217ValPro: 2.217 ± 0.182
1.971ValGln: 1.971 ± 0.195
2.171ValArg: 2.171 ± 0.201
4.018ValSer: 4.018 ± 0.251
2.663ValThr: 2.663 ± 0.226
3.141ValVal: 3.141 ± 0.246
0.477ValTrp: 0.477 ± 0.081
1.509ValTyr: 1.509 ± 0.142
0.0ValXaa: 0.0 ± 0.0
Trp
0.831TrpAla: 0.831 ± 0.107
0.123TrpCys: 0.123 ± 0.042
0.708TrpAsp: 0.708 ± 0.114
0.754TrpGlu: 0.754 ± 0.109
0.462TrpPhe: 0.462 ± 0.095
0.647TrpGly: 0.647 ± 0.107
0.108TrpHis: 0.108 ± 0.041
0.985TrpIle: 0.985 ± 0.138
0.785TrpLys: 0.785 ± 0.146
0.924TrpLeu: 0.924 ± 0.137
0.369TrpMet: 0.369 ± 0.084
0.724TrpAsn: 0.724 ± 0.116
0.539TrpPro: 0.539 ± 0.141
0.416TrpGln: 0.416 ± 0.076
0.446TrpArg: 0.446 ± 0.082
0.539TrpSer: 0.539 ± 0.092
0.493TrpThr: 0.493 ± 0.083
0.831TrpVal: 0.831 ± 0.105
0.108TrpTrp: 0.108 ± 0.048
0.323TrpTyr: 0.323 ± 0.068
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.155TyrAla: 2.155 ± 0.182
0.585TyrCys: 0.585 ± 0.091
2.017TyrAsp: 2.017 ± 0.177
2.155TyrGlu: 2.155 ± 0.211
2.217TyrPhe: 2.217 ± 0.182
1.971TyrGly: 1.971 ± 0.177
0.724TyrHis: 0.724 ± 0.105
2.802TyrIle: 2.802 ± 0.23
1.924TyrLys: 1.924 ± 0.194
3.448TyrLeu: 3.448 ± 0.248
0.816TyrMet: 0.816 ± 0.139
1.786TyrAsn: 1.786 ± 0.148
1.509TyrPro: 1.509 ± 0.167
1.339TyrGln: 1.339 ± 0.131
1.447TyrArg: 1.447 ± 0.164
2.248TyrSer: 2.248 ± 0.204
1.693TyrThr: 1.693 ± 0.168
1.74TyrVal: 1.74 ± 0.176
0.508TyrTrp: 0.508 ± 0.095
1.632TyrTyr: 1.632 ± 0.162
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 203 proteins (64958 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski