Phaeocystidibacter luteus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Schleiferiaceae; Phaeocystidibacter

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2801 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N6RLG2|A0A6N6RLG2_9FLAO Succinate--CoA ligase [ADP-forming] subunit beta OS=Phaeocystidibacter luteus OX=911197 GN=sucC PE=3 SV=1
MM1 pKa = 7.71NYY3 pKa = 8.73TKK5 pKa = 10.79SFLIRR10 pKa = 11.84SMVAVAALGSFTACEE25 pKa = 4.0DD26 pKa = 3.59LSLCGLTEE34 pKa = 4.41EE35 pKa = 4.85DD36 pKa = 5.03LSMGDD41 pKa = 3.64AFVEE45 pKa = 4.51TTSYY49 pKa = 10.92FMNVVGRR56 pKa = 11.84TDD58 pKa = 2.83EE59 pKa = 4.1AMRR62 pKa = 11.84NEE64 pKa = 3.88NLQVNGSTTIDD75 pKa = 3.48GATVTRR81 pKa = 11.84TNDD84 pKa = 3.12SITIDD89 pKa = 3.55FGSNNVVTNDD99 pKa = 2.58GKK101 pKa = 8.7TRR103 pKa = 11.84RR104 pKa = 11.84GVIRR108 pKa = 11.84GYY110 pKa = 10.46ISGDD114 pKa = 3.24YY115 pKa = 9.87FQANGSINLSLEE127 pKa = 4.14NYY129 pKa = 8.74HH130 pKa = 7.03VNDD133 pKa = 3.65SLIAGSISLVNNGNNGGQDD152 pKa = 2.95PWDD155 pKa = 3.72INLSSTNFSIGNQYY169 pKa = 10.97DD170 pKa = 3.55YY171 pKa = 11.48SANLTMQWEE180 pKa = 4.3AGYY183 pKa = 8.37EE184 pKa = 4.43TIDD187 pKa = 3.33STADD191 pKa = 3.18DD192 pKa = 3.75RR193 pKa = 11.84FKK195 pKa = 11.04IFGTANGDD203 pKa = 3.93DD204 pKa = 4.4NIQLISFATSFPSPMVFDD222 pKa = 4.71RR223 pKa = 11.84SCTYY227 pKa = 10.82LVTEE231 pKa = 4.47GSVDD235 pKa = 3.38VSLTSGEE242 pKa = 4.35AEE244 pKa = 4.04VASVNVDD251 pKa = 3.87FLDD254 pKa = 4.38SDD256 pKa = 3.91GCNNVLMLNASCDD269 pKa = 3.41GTNISFPQNFDD280 pKa = 3.24GFF282 pKa = 4.04

Molecular weight: 30.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N6RGI9|A0A6N6RGI9_9FLAO ATP-binding cassette domain-containing protein OS=Phaeocystidibacter luteus OX=911197 GN=F8C67_09570 PE=4 SV=1
MM1 pKa = 7.61GVRR4 pKa = 11.84KK5 pKa = 10.07LKK7 pKa = 9.13PTSPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNEE20 pKa = 3.94FSAVTTSTPEE30 pKa = 3.58KK31 pKa = 10.67SLLKK35 pKa = 10.25GKK37 pKa = 9.9KK38 pKa = 9.65RR39 pKa = 11.84SGGRR43 pKa = 11.84NNSGKK48 pKa = 8.26MTMRR52 pKa = 11.84YY53 pKa = 9.36IGGGHH58 pKa = 6.07KK59 pKa = 10.04KK60 pKa = 9.92SYY62 pKa = 10.7RR63 pKa = 11.84EE64 pKa = 3.53IDD66 pKa = 3.7FKK68 pKa = 10.97RR69 pKa = 11.84DD70 pKa = 3.08KK71 pKa = 11.42ANIPAVVKK79 pKa = 9.92TIEE82 pKa = 3.94YY83 pKa = 10.31DD84 pKa = 3.46PNRR87 pKa = 11.84TAFIALLNYY96 pKa = 9.74VDD98 pKa = 3.65GEE100 pKa = 4.02KK101 pKa = 10.32RR102 pKa = 11.84YY103 pKa = 10.22IIAPNGLQVGQEE115 pKa = 4.2VVSGSNVAPEE125 pKa = 4.7VGNSMPLSDD134 pKa = 4.54IPLGTVVSCIEE145 pKa = 3.92LRR147 pKa = 11.84PGQGAAIARR156 pKa = 11.84SAGSFAQLLARR167 pKa = 11.84DD168 pKa = 3.86GKK170 pKa = 10.29YY171 pKa = 10.63AILKK175 pKa = 8.04MPSGEE180 pKa = 3.88TRR182 pKa = 11.84MVLITCMATIGVVSNGDD199 pKa = 3.4HH200 pKa = 5.2QQVVSGKK207 pKa = 9.94AGRR210 pKa = 11.84NRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84GVAMNPVDD229 pKa = 3.68HH230 pKa = 7.13PMGGGEE236 pKa = 3.89GRR238 pKa = 11.84ASGGHH243 pKa = 5.4PRR245 pKa = 11.84SRR247 pKa = 11.84TGVPAKK253 pKa = 10.16GYY255 pKa = 7.56KK256 pKa = 8.87TRR258 pKa = 11.84TPKK261 pKa = 10.4KK262 pKa = 9.2ASNRR266 pKa = 11.84YY267 pKa = 8.82IIEE270 pKa = 3.97RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.17KK274 pKa = 9.82

Molecular weight: 29.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2801

0

2801

972285

38

3987

347.1

38.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.283 ± 0.055

0.722 ± 0.017

5.933 ± 0.032

6.889 ± 0.06

4.772 ± 0.038

7.304 ± 0.053

2.013 ± 0.023

6.542 ± 0.038

4.931 ± 0.063

9.095 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.026

4.856 ± 0.041

3.91 ± 0.031

3.428 ± 0.026

4.772 ± 0.044

6.983 ± 0.056

5.79 ± 0.073

6.953 ± 0.039

1.419 ± 0.022

3.888 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski