Atopobium minutum 10063974

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Atopobiaceae; Atopobium; Atopobium minutum

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1539 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N2BTA6|N2BTA6_9ACTN Cell division protein FtsL OS=Atopobium minutum 10063974 OX=997872 GN=HMPREF1091_00709 PE=4 SV=1
MM1 pKa = 7.14SQLEE5 pKa = 4.14ALVVKK10 pKa = 10.81VDD12 pKa = 4.54DD13 pKa = 4.21RR14 pKa = 11.84LANLGDD20 pKa = 3.72TLSLDD25 pKa = 3.3GHH27 pKa = 6.94IDD29 pKa = 3.49QEE31 pKa = 4.71SFSLGDD37 pKa = 3.47HH38 pKa = 6.01TFMLPEE44 pKa = 5.14GISYY48 pKa = 11.04NLVLTNAGEE57 pKa = 4.76GILVTGIVKK66 pKa = 10.09AQAEE70 pKa = 4.83GVCDD74 pKa = 3.58RR75 pKa = 11.84CLDD78 pKa = 4.07PAHH81 pKa = 7.23LDD83 pKa = 3.48LSAEE87 pKa = 3.82VDD89 pKa = 3.51EE90 pKa = 4.91YY91 pKa = 11.65FLFEE95 pKa = 4.06EE96 pKa = 4.58PQAISEE102 pKa = 4.23EE103 pKa = 4.21QSDD106 pKa = 4.03EE107 pKa = 4.28EE108 pKa = 4.35EE109 pKa = 4.51GVDD112 pKa = 4.42FSLVSDD118 pKa = 4.21EE119 pKa = 4.35DD120 pKa = 4.34TIDD123 pKa = 3.34VSEE126 pKa = 4.71ALLSALLMEE135 pKa = 4.77TPYY138 pKa = 10.56IVLCRR143 pKa = 11.84EE144 pKa = 4.18DD145 pKa = 4.99CKK147 pKa = 11.17GLCPNCGANLNEE159 pKa = 4.07QDD161 pKa = 4.41CGCVAKK167 pKa = 10.71KK168 pKa = 10.4EE169 pKa = 4.01EE170 pKa = 4.04QAALEE175 pKa = 4.6GPFAALGKK183 pKa = 10.34LDD185 pKa = 5.41LNDD188 pKa = 3.39

Molecular weight: 20.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N2C0J7|N2C0J7_9ACTN Uncharacterized protein OS=Atopobium minutum 10063974 OX=997872 GN=HMPREF1091_00257 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.3QPNKK9 pKa = 8.42RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84AKK14 pKa = 8.97THH16 pKa = 5.3GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.24AGRR28 pKa = 11.84DD29 pKa = 3.41VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1539

0

1539

511244

32

2291

332.2

36.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.335 ± 0.069

1.425 ± 0.027

5.989 ± 0.051

5.938 ± 0.061

3.672 ± 0.04

7.147 ± 0.049

2.167 ± 0.029

5.88 ± 0.058

4.826 ± 0.055

9.481 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.029

3.505 ± 0.04

3.975 ± 0.039

3.998 ± 0.043

4.821 ± 0.056

6.615 ± 0.053

6.053 ± 0.052

7.513 ± 0.048

1.034 ± 0.026

3.049 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski