bacterium F16

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 688 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202DNQ0|A0A202DNQ0_9BACT Flagellin OS=bacterium F16 OX=1932694 GN=BVX99_02170 PE=3 SV=1
MM1 pKa = 7.45NGNAGTFSSHH11 pKa = 5.44WLNSLANSDD20 pKa = 3.86GPDD23 pKa = 3.28AVQHH27 pKa = 5.11EE28 pKa = 4.77TAGPGYY34 pKa = 9.59FWNLGPTGIRR44 pKa = 11.84AVLTDD49 pKa = 3.46TNGIIAEE56 pKa = 4.39GVPAGQFIVKK66 pKa = 9.48YY67 pKa = 8.67VFPGSPADD75 pKa = 5.0GIIQVDD81 pKa = 4.32DD82 pKa = 4.68IITGVNAQPFTTSYY96 pKa = 9.66TYY98 pKa = 10.72EE99 pKa = 4.16SNIEE103 pKa = 3.93YY104 pKa = 10.48GFTGPIIAIGNAIEE118 pKa = 4.59ASEE121 pKa = 4.31TNLGGVLTFTVTRR134 pKa = 11.84GGTTSEE140 pKa = 4.0MSVTLDD146 pKa = 3.15NKK148 pKa = 10.01GTFTSTFPYY157 pKa = 10.13SCPKK161 pKa = 10.32SQLLRR166 pKa = 11.84DD167 pKa = 3.83DD168 pKa = 5.63CITEE172 pKa = 4.11LLSLQKK178 pKa = 11.06SNGPWPGGGHH188 pKa = 5.43TSWFALLALMAQGTTHH204 pKa = 7.11LAAVEE209 pKa = 4.24KK210 pKa = 10.78EE211 pKa = 3.87LDD213 pKa = 3.63RR214 pKa = 11.84VSNPVDD220 pKa = 3.54LGTNNWMLAMRR231 pKa = 11.84GIAMAEE237 pKa = 4.03HH238 pKa = 6.17QLLTNSGRR246 pKa = 11.84YY247 pKa = 8.96LDD249 pKa = 4.59AMTAISNQLVANQTKK264 pKa = 9.95YY265 pKa = 10.74GNGAFGHH272 pKa = 6.14SGGLTPGVEE281 pKa = 4.01LGYY284 pKa = 11.26GPMSGITSLVLLSWVMMEE302 pKa = 4.15KK303 pKa = 10.65AGIAVNQVAMKK314 pKa = 8.85KK315 pKa = 7.36TAAVLDD321 pKa = 3.4MRR323 pKa = 11.84LPVNTLGRR331 pKa = 11.84YY332 pKa = 8.52SYY334 pKa = 11.14GWGIQTEE341 pKa = 4.71YY342 pKa = 9.12PTATVDD348 pKa = 3.38RR349 pKa = 11.84PEE351 pKa = 4.25VVASLGDD358 pKa = 3.55LHH360 pKa = 7.96ADD362 pKa = 3.23TATYY366 pKa = 10.62LDD368 pKa = 4.0GVRR371 pKa = 11.84EE372 pKa = 4.02LTIADD377 pKa = 4.64GYY379 pKa = 11.43AGIIHH384 pKa = 6.75SIWPWQPYY392 pKa = 9.08SDD394 pKa = 5.0EE395 pKa = 4.38ISTHH399 pKa = 5.92HH400 pKa = 6.64SNHH403 pKa = 6.51LARR406 pKa = 11.84CRR408 pKa = 11.84RR409 pKa = 11.84VLVNGHH415 pKa = 6.22GSAAMTINGAFLNLIIAGNVGNTSLLSLAMQEE447 pKa = 4.52NKK449 pKa = 10.72ALLNTSRR456 pKa = 11.84CVDD459 pKa = 2.98GGMYY463 pKa = 7.47MQPTRR468 pKa = 11.84DD469 pKa = 3.27HH470 pKa = 7.0SGTDD474 pKa = 3.34LPRR477 pKa = 11.84GSRR480 pKa = 11.84TLATSIWALIFSAPDD495 pKa = 2.88KK496 pKa = 11.07GLYY499 pKa = 10.2LLGRR503 pKa = 11.84GFSDD507 pKa = 4.06AVNEE511 pKa = 4.06APIAYY516 pKa = 8.24YY517 pKa = 10.37QSLQTQEE524 pKa = 3.71ATEE527 pKa = 4.15RR528 pKa = 11.84PITLDD533 pKa = 3.39ANDD536 pKa = 3.92VEE538 pKa = 5.08RR539 pKa = 11.84SSLTYY544 pKa = 10.43SVVSGPSNGIITGSGSSISYY564 pKa = 9.29TPDD567 pKa = 3.25DD568 pKa = 4.46GFAGTDD574 pKa = 3.09SFTFKK579 pKa = 11.17ANDD582 pKa = 3.74GDD584 pKa = 4.25DD585 pKa = 4.06DD586 pKa = 5.73SNIAVVDD593 pKa = 3.16ITVYY597 pKa = 10.58RR598 pKa = 11.84NVFVSVEE605 pKa = 4.02ATDD608 pKa = 4.87DD609 pKa = 3.59VALEE613 pKa = 4.31RR614 pKa = 11.84GTDD617 pKa = 3.21TGAWTVSRR625 pKa = 11.84TGDD628 pKa = 3.66TISPLVIPYY637 pKa = 7.0TTSGTAVGGVDD648 pKa = 4.07YY649 pKa = 10.86LALPDD654 pKa = 4.42SVTIPAGQSSATLTLTPIDD673 pKa = 3.65DD674 pKa = 3.99LVYY677 pKa = 10.77NEE679 pKa = 4.45PDD681 pKa = 3.15EE682 pKa = 4.63TVVLTVTDD690 pKa = 3.88TPEE693 pKa = 4.36LFTIAAGEE701 pKa = 4.04ATVWIDD707 pKa = 5.15DD708 pKa = 4.21NDD710 pKa = 3.99PNAAPTMTFNSPTTDD725 pKa = 3.29VIAIPSTVGLMLDD738 pKa = 3.52VTVTDD743 pKa = 4.94DD744 pKa = 5.51GIPLIPGALTISWTQISGPGTATFEE769 pKa = 4.59SPTAASTAVTFDD781 pKa = 3.67LDD783 pKa = 3.59GTYY786 pKa = 10.08MLTLTADD793 pKa = 4.07DD794 pKa = 4.58SDD796 pKa = 4.0LASTQRR802 pKa = 11.84IVVFVGGVGPGLWYY816 pKa = 10.82GDD818 pKa = 3.23VGGNIDD824 pKa = 4.54LTTPNPKK831 pKa = 9.15TDD833 pKa = 3.48VLVDD837 pKa = 4.01PSSKK841 pKa = 9.85TEE843 pKa = 4.07DD844 pKa = 3.8AIALNTTEE852 pKa = 5.82IYY854 pKa = 10.01TGWIYY859 pKa = 11.13DD860 pKa = 3.44ADD862 pKa = 4.18GNISFTGIIDD872 pKa = 3.69DD873 pKa = 4.19KK874 pKa = 11.43LRR876 pKa = 11.84IFIDD880 pKa = 4.05GALVLSNEE888 pKa = 4.01SRR890 pKa = 11.84NRR892 pKa = 11.84ASTSNLNLTPGWHH905 pKa = 7.26DD906 pKa = 3.07IEE908 pKa = 4.41IRR910 pKa = 11.84ISNGHH915 pKa = 6.17SGSGPINAPGIGYY928 pKa = 10.11DD929 pKa = 4.0PDD931 pKa = 5.8GGTAWQVLTDD941 pKa = 4.28PGDD944 pKa = 3.66GSFLQASFNGNIGPDD959 pKa = 3.4VTATSTSDD967 pKa = 3.25GSNGDD972 pKa = 3.7PMSISATVSDD982 pKa = 5.36DD983 pKa = 3.96NLPTPPAAVATQWLQVPDD1001 pKa = 5.02DD1002 pKa = 4.4GPLTFVDD1009 pKa = 3.56ATSITTEE1016 pKa = 4.24VACMSAATYY1025 pKa = 7.39TLRR1028 pKa = 11.84LIANDD1033 pKa = 4.03GQVTTFDD1040 pKa = 3.51DD1041 pKa = 3.88HH1042 pKa = 6.87GVTFASATRR1051 pKa = 11.84SVSATATDD1059 pKa = 3.48DD1060 pKa = 3.51TAAEE1064 pKa = 4.41TGSEE1068 pKa = 4.09TGTWTITRR1076 pKa = 11.84NGDD1079 pKa = 3.17LSAGLTVYY1087 pKa = 10.82YY1088 pKa = 10.54SLGGTASAADD1098 pKa = 3.77YY1099 pKa = 9.43TVTPLNSVTMAPNAASATVTLTPIDD1124 pKa = 4.27DD1125 pKa = 4.17ALKK1128 pKa = 10.2EE1129 pKa = 4.18GPEE1132 pKa = 4.23TVEE1135 pKa = 4.16LTITPDD1141 pKa = 2.9AAYY1144 pKa = 10.09FSDD1147 pKa = 3.58ATASTLTIVDD1157 pKa = 4.35SDD1159 pKa = 3.78NQAPIVNAGPDD1170 pKa = 3.39QPVQLVAVPLPPGWSSAAWTGDD1192 pKa = 3.12ADD1194 pKa = 4.28SGIDD1198 pKa = 4.04NEE1200 pKa = 4.55LTYY1203 pKa = 10.16TAAHH1207 pKa = 6.45CFGNNHH1213 pKa = 6.91AGTVTVDD1220 pKa = 2.72RR1221 pKa = 11.84VEE1223 pKa = 4.25FTEE1226 pKa = 5.04SFDD1229 pKa = 3.61TTGTGWSITGGKK1241 pKa = 9.82ANWDD1245 pKa = 3.84GNDD1248 pKa = 4.03DD1249 pKa = 5.22AILTDD1254 pKa = 3.77GSEE1257 pKa = 4.24DD1258 pKa = 3.36LGEE1261 pKa = 3.96EE1262 pKa = 4.77FIYY1265 pKa = 10.63GGNPRR1270 pKa = 11.84TVTFTGLTPGDD1281 pKa = 4.24SYY1283 pKa = 11.48TATFFSVGWDD1293 pKa = 3.44TTASRR1298 pKa = 11.84EE1299 pKa = 4.02QTFSITGGSSLLVDD1313 pKa = 3.37QNSYY1317 pKa = 9.28GKK1319 pKa = 10.19NYY1321 pKa = 9.85GISVSCNYY1329 pKa = 8.19TATATSQEE1337 pKa = 4.31VVIAPVAGSFHH1348 pKa = 7.26LYY1350 pKa = 10.94AMANADD1356 pKa = 3.88TNMLQATAPLSGTVTDD1372 pKa = 5.43DD1373 pKa = 5.61DD1374 pKa = 5.35GDD1376 pKa = 3.93TPSAIWSLVDD1386 pKa = 3.87GPAPVTFDD1394 pKa = 4.21DD1395 pKa = 4.32ASAMITNAYY1404 pKa = 7.51FTEE1407 pKa = 4.64AGTYY1411 pKa = 7.09TLRR1414 pKa = 11.84LTADD1418 pKa = 3.52DD1419 pKa = 4.27TFEE1422 pKa = 4.03PAYY1425 pKa = 10.27DD1426 pKa = 3.61DD1427 pKa = 4.14VIIIVGTGNEE1437 pKa = 4.41IYY1439 pKa = 10.71SVTYY1443 pKa = 10.49DD1444 pKa = 3.31GNTNDD1449 pKa = 4.6GGAIPTGSVDD1459 pKa = 3.43YY1460 pKa = 11.07NFNEE1464 pKa = 4.28VVTVLDD1470 pKa = 3.43NTGNLTKK1477 pKa = 10.16TGYY1480 pKa = 8.67TFTGWNTAANGSGAIYY1496 pKa = 8.95TAGDD1500 pKa = 3.76TFVITADD1507 pKa = 3.52TVLYY1511 pKa = 9.42AQWSINSYY1519 pKa = 10.86AVTYY1523 pKa = 10.37DD1524 pKa = 3.68GNSADD1529 pKa = 4.11NGTVPAVQTKK1539 pKa = 10.21EE1540 pKa = 3.78YY1541 pKa = 10.53GADD1544 pKa = 2.73ITLAFNTGNLSQSGLTFAGWNTASDD1569 pKa = 3.95DD1570 pKa = 4.08SGTHH1574 pKa = 4.59YY1575 pKa = 11.36AEE1577 pKa = 4.43GATYY1581 pKa = 10.88SDD1583 pKa = 3.69NTDD1586 pKa = 2.98LTLYY1590 pKa = 10.61AEE1592 pKa = 4.49WSSVPVYY1599 pKa = 11.01SLTVNNGTGTGLYY1612 pKa = 8.16EE1613 pKa = 4.56ANDD1616 pKa = 3.82VVSVEE1621 pKa = 3.99ASIPANYY1628 pKa = 10.12HH1629 pKa = 4.5FVNWSGDD1636 pKa = 3.25TAAVGDD1642 pKa = 4.24VNSSSTMVTMPAADD1656 pKa = 3.49VTITANVAIDD1666 pKa = 3.88TYY1668 pKa = 11.6AVTFVEE1674 pKa = 4.95GANGTRR1680 pKa = 11.84TGGGALVQTIDD1691 pKa = 3.6YY1692 pKa = 9.92GSAATAPVITADD1704 pKa = 3.41SGYY1707 pKa = 8.85TFTGWDD1713 pKa = 2.96IAFEE1717 pKa = 4.24NVTSNLTVAAQYY1729 pKa = 11.15SVDD1732 pKa = 3.57AVGGSSGGGGGGGGGCQMGVVTGGSLLPFALLLVVAGFIRR1772 pKa = 11.84RR1773 pKa = 11.84RR1774 pKa = 11.84FF1775 pKa = 3.4

Molecular weight: 185.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202DPI5|A0A202DPI5_9BACT Uncharacterized protein OS=bacterium F16 OX=1932694 GN=BVX99_01260 PE=4 SV=1
MM1 pKa = 7.19HH2 pKa = 7.64ANMAEE7 pKa = 4.53AIVMADD13 pKa = 3.52VLSGQALGRR22 pKa = 11.84RR23 pKa = 11.84AFEE26 pKa = 3.95TLFVAEE32 pKa = 4.71VGSSPMNHH40 pKa = 6.16LRR42 pKa = 11.84SIRR45 pKa = 11.84LRR47 pKa = 11.84RR48 pKa = 11.84IRR50 pKa = 11.84YY51 pKa = 8.29LLRR54 pKa = 11.84TTDD57 pKa = 3.63LTVKK61 pKa = 10.52EE62 pKa = 4.66IGRR65 pKa = 11.84QCGIPNITYY74 pKa = 10.19LCRR77 pKa = 11.84LFQKK81 pKa = 10.1IHH83 pKa = 5.79GVTPKK88 pKa = 10.23QYY90 pKa = 10.28RR91 pKa = 11.84EE92 pKa = 3.82QRR94 pKa = 3.37

Molecular weight: 10.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

688

0

688

212882

46

2165

309.4

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.882 ± 0.103

1.364 ± 0.042

6.194 ± 0.071

6.263 ± 0.091

4.035 ± 0.062

7.086 ± 0.098

2.238 ± 0.046

6.443 ± 0.067

5.61 ± 0.119

9.721 ± 0.118

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.045

4.129 ± 0.054

4.473 ± 0.077

3.447 ± 0.064

5.354 ± 0.081

6.479 ± 0.085

5.948 ± 0.149

6.468 ± 0.08

1.234 ± 0.04

3.055 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski