Amino acid dipepetide frequency for bacterium F16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.764AlaAla: 6.764 ± 0.241
1.066AlaCys: 1.066 ± 0.068
4.843AlaAsp: 4.843 ± 0.177
5.421AlaGlu: 5.421 ± 0.191
3.321AlaPhe: 3.321 ± 0.126
5.877AlaGly: 5.877 ± 0.199
1.536AlaHis: 1.536 ± 0.089
5.195AlaIle: 5.195 ± 0.15
3.894AlaLys: 3.894 ± 0.171
8.004AlaLeu: 8.004 ± 0.218
2.236AlaMet: 2.236 ± 0.11
2.931AlaAsn: 2.931 ± 0.126
3.044AlaPro: 3.044 ± 0.135
2.823AlaGln: 2.823 ± 0.148
3.673AlaArg: 3.673 ± 0.125
4.613AlaSer: 4.613 ± 0.189
4.604AlaThr: 4.604 ± 0.283
5.745AlaVal: 5.745 ± 0.173
0.968AlaTrp: 0.968 ± 0.074
2.264AlaTyr: 2.264 ± 0.128
0.0AlaXaa: 0.0 ± 0.0
Cys
0.846CysAla: 0.846 ± 0.067
0.263CysCys: 0.263 ± 0.039
0.864CysAsp: 0.864 ± 0.065
0.686CysGlu: 0.686 ± 0.068
0.573CysPhe: 0.573 ± 0.058
1.212CysGly: 1.212 ± 0.08
0.39CysHis: 0.39 ± 0.046
0.897CysIle: 0.897 ± 0.067
0.7CysLys: 0.7 ± 0.057
1.381CysLeu: 1.381 ± 0.082
0.348CysMet: 0.348 ± 0.04
0.531CysAsn: 0.531 ± 0.053
0.662CysPro: 0.662 ± 0.063
0.432CysGln: 0.432 ± 0.051
0.803CysArg: 0.803 ± 0.065
1.043CysSer: 1.043 ± 0.083
0.601CysThr: 0.601 ± 0.055
0.789CysVal: 0.789 ± 0.072
0.146CysTrp: 0.146 ± 0.025
0.474CysTyr: 0.474 ± 0.046
0.0CysXaa: 0.0 ± 0.0
Asp
5.252AspAla: 5.252 ± 0.226
0.78AspCys: 0.78 ± 0.066
4.787AspAsp: 4.787 ± 0.237
4.317AspGlu: 4.317 ± 0.165
2.414AspPhe: 2.414 ± 0.108
4.829AspGly: 4.829 ± 0.201
1.273AspHis: 1.273 ± 0.074
4.021AspIle: 4.021 ± 0.154
2.973AspLys: 2.973 ± 0.128
5.75AspLeu: 5.75 ± 0.182
1.597AspMet: 1.597 ± 0.081
2.236AspAsn: 2.236 ± 0.112
2.673AspPro: 2.673 ± 0.107
1.931AspGln: 1.931 ± 0.091
2.795AspArg: 2.795 ± 0.134
3.786AspSer: 3.786 ± 0.146
3.124AspThr: 3.124 ± 0.173
4.284AspVal: 4.284 ± 0.154
0.803AspTrp: 0.803 ± 0.072
2.311AspTyr: 2.311 ± 0.116
0.0AspXaa: 0.0 ± 0.0
Glu
5.069GluAla: 5.069 ± 0.17
0.761GluCys: 0.761 ± 0.062
3.373GluAsp: 3.373 ± 0.126
3.716GluGlu: 3.716 ± 0.173
2.579GluPhe: 2.579 ± 0.111
3.749GluGly: 3.749 ± 0.147
1.517GluHis: 1.517 ± 0.104
3.979GluIle: 3.979 ± 0.165
4.19GluLys: 4.19 ± 0.184
6.849GluLeu: 6.849 ± 0.206
1.79GluMet: 1.79 ± 0.116
2.725GluAsn: 2.725 ± 0.118
2.184GluPro: 2.184 ± 0.107
2.1GluGln: 2.1 ± 0.095
3.777GluArg: 3.777 ± 0.164
3.941GluSer: 3.941 ± 0.159
4.134GluThr: 4.134 ± 0.153
3.645GluVal: 3.645 ± 0.16
0.709GluTrp: 0.709 ± 0.063
1.837GluTyr: 1.837 ± 0.093
0.0GluXaa: 0.0 ± 0.0
Phe
2.927PheAla: 2.927 ± 0.117
0.766PheCys: 0.766 ± 0.062
2.748PheAsp: 2.748 ± 0.125
2.541PheGlu: 2.541 ± 0.123
1.809PhePhe: 1.809 ± 0.113
2.8PheGly: 2.8 ± 0.125
0.831PheHis: 0.831 ± 0.063
2.663PheIle: 2.663 ± 0.114
1.987PheLys: 1.987 ± 0.113
3.885PheLeu: 3.885 ± 0.154
0.921PheMet: 0.921 ± 0.066
1.729PheAsn: 1.729 ± 0.099
1.55PhePro: 1.55 ± 0.086
1.231PheGln: 1.231 ± 0.096
2.189PheArg: 2.189 ± 0.11
3.119PheSer: 3.119 ± 0.113
2.452PheThr: 2.452 ± 0.114
2.269PheVal: 2.269 ± 0.102
0.55PheTrp: 0.55 ± 0.063
1.386PheTyr: 1.386 ± 0.099
0.0PheXaa: 0.0 ± 0.0
Gly
5.003GlyAla: 5.003 ± 0.199
1.09GlyCys: 1.09 ± 0.069
4.303GlyAsp: 4.303 ± 0.148
4.068GlyGlu: 4.068 ± 0.144
2.654GlyPhe: 2.654 ± 0.107
5.134GlyGly: 5.134 ± 0.256
1.733GlyHis: 1.733 ± 0.102
4.932GlyIle: 4.932 ± 0.153
4.622GlyLys: 4.622 ± 0.192
6.431GlyLeu: 6.431 ± 0.182
2.194GlyMet: 2.194 ± 0.103
3.34GlyAsn: 3.34 ± 0.154
2.123GlyPro: 2.123 ± 0.112
2.438GlyGln: 2.438 ± 0.131
3.946GlyArg: 3.946 ± 0.127
4.453GlySer: 4.453 ± 0.186
4.359GlyThr: 4.359 ± 0.247
4.43GlyVal: 4.43 ± 0.18
1.015GlyTrp: 1.015 ± 0.089
2.588GlyTyr: 2.588 ± 0.119
0.0GlyXaa: 0.0 ± 0.0
His
1.663HisAla: 1.663 ± 0.1
0.395HisCys: 0.395 ± 0.047
1.296HisAsp: 1.296 ± 0.077
1.245HisGlu: 1.245 ± 0.071
0.986HisPhe: 0.986 ± 0.07
1.654HisGly: 1.654 ± 0.083
0.756HisHis: 0.756 ± 0.06
1.452HisIle: 1.452 ± 0.1
1.052HisLys: 1.052 ± 0.069
2.288HisLeu: 2.288 ± 0.099
0.564HisMet: 0.564 ± 0.05
0.808HisAsn: 0.808 ± 0.061
1.358HisPro: 1.358 ± 0.083
0.719HisGln: 0.719 ± 0.06
1.32HisArg: 1.32 ± 0.09
1.372HisSer: 1.372 ± 0.075
1.038HisThr: 1.038 ± 0.066
1.311HisVal: 1.311 ± 0.083
0.287HisTrp: 0.287 ± 0.036
0.817HisTyr: 0.817 ± 0.062
0.0HisXaa: 0.0 ± 0.0
Ile
5.322IleAla: 5.322 ± 0.192
0.888IleCys: 0.888 ± 0.063
3.988IleAsp: 3.988 ± 0.135
3.81IleGlu: 3.81 ± 0.151
2.405IlePhe: 2.405 ± 0.119
4.392IleGly: 4.392 ± 0.148
1.367IleHis: 1.367 ± 0.095
4.289IleIle: 4.289 ± 0.165
3.373IleLys: 3.373 ± 0.142
6.13IleLeu: 6.13 ± 0.203
1.442IleMet: 1.442 ± 0.102
2.668IleAsn: 2.668 ± 0.109
3.143IlePro: 3.143 ± 0.128
2.306IleGln: 2.306 ± 0.106
3.65IleArg: 3.65 ± 0.162
4.702IleSer: 4.702 ± 0.184
4.284IleThr: 4.284 ± 0.24
3.716IleVal: 3.716 ± 0.128
0.817IleTrp: 0.817 ± 0.069
1.743IleTyr: 1.743 ± 0.103
0.0IleXaa: 0.0 ± 0.0
Lys
4.585LysAla: 4.585 ± 0.193
0.564LysCys: 0.564 ± 0.049
3.307LysAsp: 3.307 ± 0.136
3.786LysGlu: 3.786 ± 0.164
1.39LysPhe: 1.39 ± 0.094
3.838LysGly: 3.838 ± 0.181
1.395LysHis: 1.395 ± 0.089
2.969LysIle: 2.969 ± 0.139
3.871LysLys: 3.871 ± 0.227
5.04LysLeu: 5.04 ± 0.186
1.654LysMet: 1.654 ± 0.087
2.471LysAsn: 2.471 ± 0.114
2.814LysPro: 2.814 ± 0.133
2.255LysGln: 2.255 ± 0.115
3.471LysArg: 3.471 ± 0.149
3.227LysSer: 3.227 ± 0.137
3.547LysThr: 3.547 ± 0.123
3.617LysVal: 3.617 ± 0.166
0.794LysTrp: 0.794 ± 0.069
1.503LysTyr: 1.503 ± 0.09
0.0LysXaa: 0.0 ± 0.0
Leu
8.061LeuAla: 8.061 ± 0.219
1.264LeuCys: 1.264 ± 0.084
5.74LeuAsp: 5.74 ± 0.173
6.398LeuGlu: 6.398 ± 0.203
3.913LeuPhe: 3.913 ± 0.152
6.426LeuGly: 6.426 ± 0.206
2.02LeuHis: 2.02 ± 0.095
6.182LeuIle: 6.182 ± 0.17
6.417LeuLys: 6.417 ± 0.208
9.465LeuLeu: 9.465 ± 0.257
2.372LeuMet: 2.372 ± 0.105
4.402LeuAsn: 4.402 ± 0.155
4.679LeuPro: 4.679 ± 0.17
3.124LeuGln: 3.124 ± 0.156
4.975LeuArg: 4.975 ± 0.181
6.91LeuSer: 6.91 ± 0.197
5.844LeuThr: 5.844 ± 0.275
5.783LeuVal: 5.783 ± 0.177
0.897LeuTrp: 0.897 ± 0.07
2.339LeuTyr: 2.339 ± 0.123
0.0LeuXaa: 0.0 ± 0.0
Met
2.438MetAla: 2.438 ± 0.1
0.272MetCys: 0.272 ± 0.033
1.362MetAsp: 1.362 ± 0.083
1.574MetGlu: 1.574 ± 0.089
0.944MetPhe: 0.944 ± 0.066
1.846MetGly: 1.846 ± 0.106
0.432MetHis: 0.432 ± 0.049
1.56MetIle: 1.56 ± 0.087
1.7MetLys: 1.7 ± 0.104
2.504MetLeu: 2.504 ± 0.097
0.761MetMet: 0.761 ± 0.064
1.151MetAsn: 1.151 ± 0.07
1.306MetPro: 1.306 ± 0.076
0.756MetGln: 0.756 ± 0.059
1.358MetArg: 1.358 ± 0.088
1.724MetSer: 1.724 ± 0.102
1.78MetThr: 1.78 ± 0.082
1.588MetVal: 1.588 ± 0.087
0.15MetTrp: 0.15 ± 0.027
0.568MetTyr: 0.568 ± 0.055
0.0MetXaa: 0.0 ± 0.0
Asn
3.175AsnAla: 3.175 ± 0.137
0.512AsnCys: 0.512 ± 0.047
2.476AsnAsp: 2.476 ± 0.112
2.645AsnGlu: 2.645 ± 0.108
1.235AsnPhe: 1.235 ± 0.07
3.293AsnGly: 3.293 ± 0.148
1.029AsnHis: 1.029 ± 0.071
2.494AsnIle: 2.494 ± 0.112
1.898AsnLys: 1.898 ± 0.096
3.749AsnLeu: 3.749 ± 0.136
1.043AsnMet: 1.043 ± 0.07
1.855AsnAsn: 1.855 ± 0.089
2.405AsnPro: 2.405 ± 0.112
1.574AsnGln: 1.574 ± 0.094
2.382AsnArg: 2.382 ± 0.115
2.635AsnSer: 2.635 ± 0.113
2.419AsnThr: 2.419 ± 0.106
2.635AsnVal: 2.635 ± 0.11
0.564AsnTrp: 0.564 ± 0.059
1.268AsnTyr: 1.268 ± 0.088
0.0AsnXaa: 0.0 ± 0.0
Pro
3.617ProAla: 3.617 ± 0.211
0.446ProCys: 0.446 ± 0.045
3.316ProAsp: 3.316 ± 0.124
3.777ProGlu: 3.777 ± 0.178
2.001ProPhe: 2.001 ± 0.112
3.288ProGly: 3.288 ± 0.137
0.977ProHis: 0.977 ± 0.075
2.635ProIle: 2.635 ± 0.137
2.274ProLys: 2.274 ± 0.141
3.885ProLeu: 3.885 ± 0.146
1.019ProMet: 1.019 ± 0.074
1.654ProAsn: 1.654 ± 0.108
1.921ProPro: 1.921 ± 0.133
1.466ProGln: 1.466 ± 0.092
1.874ProArg: 1.874 ± 0.11
2.936ProSer: 2.936 ± 0.146
2.513ProThr: 2.513 ± 0.131
3.288ProVal: 3.288 ± 0.153
0.568ProTrp: 0.568 ± 0.057
1.278ProTyr: 1.278 ± 0.087
0.0ProXaa: 0.0 ± 0.0
Gln
2.978GlnAla: 2.978 ± 0.121
0.507GlnCys: 0.507 ± 0.045
1.841GlnAsp: 1.841 ± 0.085
2.166GlnGlu: 2.166 ± 0.107
1.226GlnPhe: 1.226 ± 0.074
2.053GlnGly: 2.053 ± 0.09
0.78GlnHis: 0.78 ± 0.067
2.213GlnIle: 2.213 ± 0.12
2.189GlnLys: 2.189 ± 0.106
3.636GlnLeu: 3.636 ± 0.17
0.864GlnMet: 0.864 ± 0.057
1.419GlnAsn: 1.419 ± 0.091
1.423GlnPro: 1.423 ± 0.125
1.447GlnGln: 1.447 ± 0.082
1.964GlnArg: 1.964 ± 0.102
2.175GlnSer: 2.175 ± 0.104
1.879GlnThr: 1.879 ± 0.111
2.438GlnVal: 2.438 ± 0.102
0.385GlnTrp: 0.385 ± 0.043
0.893GlnTyr: 0.893 ± 0.07
0.0GlnXaa: 0.0 ± 0.0
Arg
3.471ArgAla: 3.471 ± 0.154
0.714ArgCys: 0.714 ± 0.063
3.006ArgAsp: 3.006 ± 0.135
3.213ArgGlu: 3.213 ± 0.143
2.626ArgPhe: 2.626 ± 0.12
3.02ArgGly: 3.02 ± 0.12
1.362ArgHis: 1.362 ± 0.085
3.702ArgIle: 3.702 ± 0.147
3.495ArgLys: 3.495 ± 0.138
5.529ArgLeu: 5.529 ± 0.178
1.644ArgMet: 1.644 ± 0.097
2.184ArgAsn: 2.184 ± 0.101
2.025ArgPro: 2.025 ± 0.101
2.447ArgGln: 2.447 ± 0.119
3.044ArgArg: 3.044 ± 0.132
3.171ArgSer: 3.171 ± 0.136
2.978ArgThr: 2.978 ± 0.131
3.199ArgVal: 3.199 ± 0.131
0.695ArgTrp: 0.695 ± 0.068
2.011ArgTyr: 2.011 ± 0.1
0.0ArgXaa: 0.0 ± 0.0
Ser
4.848SerAla: 4.848 ± 0.185
0.921SerCys: 0.921 ± 0.062
3.819SerAsp: 3.819 ± 0.128
3.683SerGlu: 3.683 ± 0.142
2.95SerPhe: 2.95 ± 0.133
5.552SerGly: 5.552 ± 0.225
1.315SerHis: 1.315 ± 0.076
4.045SerIle: 4.045 ± 0.151
3.227SerLys: 3.227 ± 0.14
6.482SerLeu: 6.482 ± 0.185
1.508SerMet: 1.508 ± 0.085
2.358SerAsn: 2.358 ± 0.117
3.171SerPro: 3.171 ± 0.132
2.001SerGln: 2.001 ± 0.118
3.805SerArg: 3.805 ± 0.156
4.524SerSer: 4.524 ± 0.166
3.354SerThr: 3.354 ± 0.156
4.453SerVal: 4.453 ± 0.164
0.963SerTrp: 0.963 ± 0.069
1.809SerTyr: 1.809 ± 0.103
0.0SerXaa: 0.0 ± 0.0
Thr
4.693ThrAla: 4.693 ± 0.283
0.799ThrCys: 0.799 ± 0.068
3.838ThrAsp: 3.838 ± 0.197
3.114ThrGlu: 3.114 ± 0.133
2.476ThrPhe: 2.476 ± 0.183
4.716ThrGly: 4.716 ± 0.214
1.25ThrHis: 1.25 ± 0.07
4.012ThrIle: 4.012 ± 0.181
2.776ThrLys: 2.776 ± 0.118
6.205ThrLeu: 6.205 ± 0.255
1.273ThrMet: 1.273 ± 0.077
2.18ThrAsn: 2.18 ± 0.114
3.504ThrPro: 3.504 ± 0.187
1.78ThrGln: 1.78 ± 0.098
2.654ThrArg: 2.654 ± 0.109
3.542ThrSer: 3.542 ± 0.147
3.486ThrThr: 3.486 ± 0.219
4.702ThrVal: 4.702 ± 0.284
0.709ThrTrp: 0.709 ± 0.059
1.776ThrTyr: 1.776 ± 0.129
0.0ThrXaa: 0.0 ± 0.0
Val
5.191ValAla: 5.191 ± 0.219
0.883ValCys: 0.883 ± 0.063
4.345ValAsp: 4.345 ± 0.156
4.19ValGlu: 4.19 ± 0.137
2.875ValPhe: 2.875 ± 0.138
4.115ValGly: 4.115 ± 0.169
1.235ValHis: 1.235 ± 0.079
4.683ValIle: 4.683 ± 0.182
3.34ValLys: 3.34 ± 0.136
5.717ValLeu: 5.717 ± 0.201
1.55ValMet: 1.55 ± 0.091
2.861ValAsn: 2.861 ± 0.143
2.955ValPro: 2.955 ± 0.135
2.02ValGln: 2.02 ± 0.083
3.406ValArg: 3.406 ± 0.132
4.167ValSer: 4.167 ± 0.123
4.171ValThr: 4.171 ± 0.296
4.453ValVal: 4.453 ± 0.154
0.629ValTrp: 0.629 ± 0.061
1.893ValTyr: 1.893 ± 0.1
0.0ValXaa: 0.0 ± 0.0
Trp
0.855TrpAla: 0.855 ± 0.076
0.211TrpCys: 0.211 ± 0.029
0.639TrpAsp: 0.639 ± 0.058
0.728TrpGlu: 0.728 ± 0.064
0.484TrpPhe: 0.484 ± 0.056
0.855TrpGly: 0.855 ± 0.072
0.366TrpHis: 0.366 ± 0.045
0.714TrpIle: 0.714 ± 0.056
0.639TrpLys: 0.639 ± 0.058
1.198TrpLeu: 1.198 ± 0.075
0.348TrpMet: 0.348 ± 0.042
0.568TrpAsn: 0.568 ± 0.06
0.512TrpPro: 0.512 ± 0.048
0.531TrpGln: 0.531 ± 0.05
0.667TrpArg: 0.667 ± 0.056
0.869TrpSer: 0.869 ± 0.08
0.752TrpThr: 0.752 ± 0.061
0.756TrpVal: 0.756 ± 0.062
0.211TrpTrp: 0.211 ± 0.035
0.442TrpTyr: 0.442 ± 0.045
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.067TyrAla: 2.067 ± 0.098
0.54TyrCys: 0.54 ± 0.053
2.043TyrAsp: 2.043 ± 0.121
1.55TyrGlu: 1.55 ± 0.092
1.489TyrPhe: 1.489 ± 0.097
2.208TyrGly: 2.208 ± 0.095
0.813TyrHis: 0.813 ± 0.063
1.799TyrIle: 1.799 ± 0.098
1.48TyrLys: 1.48 ± 0.099
3.082TyrLeu: 3.082 ± 0.13
0.644TyrMet: 0.644 ± 0.053
1.217TyrAsn: 1.217 ± 0.08
1.282TyrPro: 1.282 ± 0.092
1.095TyrGln: 1.095 ± 0.074
1.785TyrArg: 1.785 ± 0.089
1.879TyrSer: 1.879 ± 0.084
2.166TyrThr: 2.166 ± 0.142
1.578TyrVal: 1.578 ± 0.088
0.484TyrTrp: 0.484 ± 0.052
1.348TyrTyr: 1.348 ± 0.076
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 688 proteins (212882 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski