Candidatus Tokpelaia sp. JSC085

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiales incertae sedis; Candidatus Tokpelaia; unclassified Candidatus Tokpelaia

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367ZXA5|A0A367ZXA5_9RHIZ Uncharacterized protein OS=Candidatus Tokpelaia sp. JSC085 OX=2079008 GN=JSC085_000956 PE=4 SV=1
MM1 pKa = 8.34IEE3 pKa = 3.92EE4 pKa = 4.36LAPYY8 pKa = 9.68IEE10 pKa = 4.67KK11 pKa = 10.18TMSANSVDD19 pKa = 4.13GQLPDD24 pKa = 4.18CQSSAPFGDD33 pKa = 3.27TGAPILLSVVV43 pKa = 3.29

Molecular weight: 4.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368A1U1|A0A368A1U1_9RHIZ DNA mismatch repair protein MutS OS=Candidatus Tokpelaia sp. JSC085 OX=2079008 GN=mutS PE=3 SV=1
MM1 pKa = 7.43NIIAKK6 pKa = 10.38LEE8 pKa = 4.21AEE10 pKa = 3.82QHH12 pKa = 6.3AIIEE16 pKa = 4.51KK17 pKa = 10.12KK18 pKa = 9.89RR19 pKa = 11.84RR20 pKa = 11.84LPFFQQGDD28 pKa = 4.11SVRR31 pKa = 11.84VLVRR35 pKa = 11.84VMEE38 pKa = 4.33GTRR41 pKa = 11.84TRR43 pKa = 11.84VQAYY47 pKa = 9.27EE48 pKa = 4.46GVCIARR54 pKa = 11.84SGGGLHH60 pKa = 5.81EE61 pKa = 4.58TFTVRR66 pKa = 11.84KK67 pKa = 8.32ISYY70 pKa = 8.79GEE72 pKa = 3.79GVEE75 pKa = 4.82RR76 pKa = 11.84VFPVCSPLIEE86 pKa = 4.14GVEE89 pKa = 4.09IIRR92 pKa = 11.84RR93 pKa = 11.84GRR95 pKa = 11.84VRR97 pKa = 11.84RR98 pKa = 11.84AKK100 pKa = 10.3LYY102 pKa = 8.84YY103 pKa = 10.15LRR105 pKa = 11.84RR106 pKa = 11.84LTGKK110 pKa = 9.93AARR113 pKa = 11.84IVEE116 pKa = 4.16KK117 pKa = 10.28RR118 pKa = 11.84DD119 pKa = 3.38KK120 pKa = 10.45NRR122 pKa = 11.84KK123 pKa = 8.62DD124 pKa = 3.73AVVCSVV130 pKa = 3.02

Molecular weight: 14.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

963

0

963

305146

30

1717

316.9

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.663 ± 0.077

1.266 ± 0.026

5.24 ± 0.054

5.585 ± 0.061

4.319 ± 0.055

7.235 ± 0.078

2.557 ± 0.039

7.945 ± 0.059

5.017 ± 0.061

9.734 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.816 ± 0.035

3.775 ± 0.043

3.979 ± 0.036

3.436 ± 0.044

6.286 ± 0.061

6.193 ± 0.055

5.28 ± 0.047

6.857 ± 0.065

1.06 ± 0.03

2.756 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski