Thermodesulfitimonas autotrophica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Thermodesulfitimonas

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2146 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N5B0W0|A0A3N5B0W0_9THEO Uncharacterized protein YrrD OS=Thermodesulfitimonas autotrophica OX=1894989 GN=EDD75_1573 PE=4 SV=1
MM1 pKa = 7.92LYY3 pKa = 10.16PEE5 pKa = 4.35EE6 pKa = 5.22DD7 pKa = 3.63KK8 pKa = 11.65YY9 pKa = 11.79SLNDD13 pKa = 3.76GEE15 pKa = 4.77EE16 pKa = 4.46GSSLFITEE24 pKa = 4.81RR25 pKa = 11.84IEE27 pKa = 5.24DD28 pKa = 3.91YY29 pKa = 10.98LALSVAAAILVIVLLFWW46 pKa = 4.4

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N5BTV1|A0A3N5BTV1_9THEO Putative regulatory protein EDD75_0100 OS=Thermodesulfitimonas autotrophica OX=1894989 GN=EDD75_0100 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.37RR3 pKa = 11.84TYY5 pKa = 9.3QPKK8 pKa = 7.96VRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.69RR14 pKa = 11.84EE15 pKa = 3.6HH16 pKa = 6.49GFLKK20 pKa = 10.4RR21 pKa = 11.84MRR23 pKa = 11.84SRR25 pKa = 11.84SGRR28 pKa = 11.84NVIKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.22GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2146

0

2146

647403

30

1939

301.7

33.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.381 ± 0.073

1.292 ± 0.027

4.221 ± 0.039

7.116 ± 0.058

3.855 ± 0.037

8.527 ± 0.047

1.706 ± 0.024

4.963 ± 0.05

4.244 ± 0.046

11.031 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.808 ± 0.022

2.491 ± 0.031

5.18 ± 0.039

2.846 ± 0.027

7.599 ± 0.065

4.009 ± 0.035

4.876 ± 0.042

8.826 ± 0.054

1.146 ± 0.029

2.883 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski