Porphyromonas sp. HMSC077F02

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas; unclassified Porphyromonas

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1F0FT18|A0A1F0FT18_9PORP Uncharacterized protein OS=Porphyromonas sp. HMSC077F02 OX=1739529 GN=HMPREF3027_08090 PE=3 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.16LASKK10 pKa = 10.73LDD12 pKa = 3.97DD13 pKa = 4.74APWPATKK20 pKa = 10.54DD21 pKa = 3.44EE22 pKa = 5.33LIDD25 pKa = 3.63YY26 pKa = 7.94AQRR29 pKa = 11.84TCAPMEE35 pKa = 4.35VIEE38 pKa = 4.27NLQEE42 pKa = 4.64IEE44 pKa = 4.46DD45 pKa = 4.5DD46 pKa = 3.75EE47 pKa = 4.55MIYY50 pKa = 11.23DD51 pKa = 4.39NIEE54 pKa = 4.5EE55 pKa = 4.25IWPDD59 pKa = 3.66YY60 pKa = 10.16PSKK63 pKa = 11.18EE64 pKa = 3.57DD65 pKa = 3.4FFFNEE70 pKa = 3.88EE71 pKa = 4.02EE72 pKa = 4.43YY73 pKa = 11.38

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1F0GAD3|A0A1F0GAD3_9PORP 3 4-dihydroxy-2-butanone 4-phosphate synthase OS=Porphyromonas sp. HMSC077F02 OX=1739529 GN=ribB PE=3 SV=1
MM1 pKa = 6.95GQKK4 pKa = 8.87TNPISNRR11 pKa = 11.84LGIIRR16 pKa = 11.84GWDD19 pKa = 3.51SNWFGKK25 pKa = 10.6KK26 pKa = 9.57SDD28 pKa = 3.97LADD31 pKa = 3.8TLLEE35 pKa = 4.52DD36 pKa = 3.93YY37 pKa = 10.78KK38 pKa = 11.25LRR40 pKa = 11.84TYY42 pKa = 10.65IEE44 pKa = 4.27ARR46 pKa = 11.84LSRR49 pKa = 11.84ASVSRR54 pKa = 11.84VVIEE58 pKa = 4.03RR59 pKa = 11.84TLKK62 pKa = 10.73LVTITICSARR72 pKa = 11.84PGHH75 pKa = 6.38IIGRR79 pKa = 11.84GGSEE83 pKa = 3.58VDD85 pKa = 3.41KK86 pKa = 11.21LRR88 pKa = 11.84EE89 pKa = 3.76EE90 pKa = 4.48LKK92 pKa = 10.76RR93 pKa = 11.84FTDD96 pKa = 3.87KK97 pKa = 10.74EE98 pKa = 4.07VQINIFEE105 pKa = 4.13IRR107 pKa = 11.84RR108 pKa = 11.84PDD110 pKa = 3.07VDD112 pKa = 3.45AKK114 pKa = 10.63IVAEE118 pKa = 4.3NIARR122 pKa = 11.84QLEE125 pKa = 3.84GRR127 pKa = 11.84MNYY130 pKa = 8.89RR131 pKa = 11.84RR132 pKa = 11.84AVKK135 pKa = 9.89QAIGNAMRR143 pKa = 11.84SGAEE147 pKa = 4.14GIKK150 pKa = 9.91IQLSGRR156 pKa = 11.84LNGAEE161 pKa = 4.19MARR164 pKa = 11.84SEE166 pKa = 4.14MFKK169 pKa = 10.52EE170 pKa = 4.38GRR172 pKa = 11.84TPLHH176 pKa = 5.9TLRR179 pKa = 11.84ADD181 pKa = 2.84IDD183 pKa = 4.03YY184 pKa = 10.92ALEE187 pKa = 3.79EE188 pKa = 4.19ARR190 pKa = 11.84TKK192 pKa = 10.85VGAIGVKK199 pKa = 9.17VWICHH204 pKa = 5.09GEE206 pKa = 4.22VYY208 pKa = 10.5GKK210 pKa = 10.34RR211 pKa = 11.84DD212 pKa = 3.6LAPDD216 pKa = 4.16FTQQRR221 pKa = 11.84DD222 pKa = 3.24NRR224 pKa = 11.84PRR226 pKa = 11.84RR227 pKa = 11.84DD228 pKa = 3.02GGRR231 pKa = 11.84GGRR234 pKa = 11.84GGRR237 pKa = 11.84RR238 pKa = 11.84RR239 pKa = 11.84GNRR242 pKa = 11.84GGRR245 pKa = 11.84NEE247 pKa = 3.84RR248 pKa = 3.68

Molecular weight: 28.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1770

0

1770

645730

38

2012

364.8

41.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.181 ± 0.059

0.846 ± 0.017

5.285 ± 0.037

7.227 ± 0.062

4.035 ± 0.032

7.06 ± 0.048

2.072 ± 0.025

6.544 ± 0.047

6.036 ± 0.045

10.117 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.569 ± 0.025

4.158 ± 0.042

4.07 ± 0.029

3.816 ± 0.03

5.07 ± 0.046

6.419 ± 0.046

5.522 ± 0.039

6.629 ± 0.044

1.15 ± 0.022

4.195 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski