Amino acid dipepetide frequency for Pseudomonas phage PaMx42

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.619AlaAla: 16.619 ± 2.854
0.802AlaCys: 0.802 ± 0.227
4.446AlaAsp: 4.446 ± 0.622
7.07AlaGlu: 7.07 ± 0.805
3.061AlaPhe: 3.061 ± 0.474
8.528AlaGly: 8.528 ± 0.899
1.822AlaHis: 1.822 ± 0.353
6.998AlaIle: 6.998 ± 0.786
6.706AlaLys: 6.706 ± 0.702
8.237AlaLeu: 8.237 ± 1.149
2.624AlaMet: 2.624 ± 0.474
6.123AlaAsn: 6.123 ± 0.821
4.446AlaPro: 4.446 ± 0.733
4.811AlaGln: 4.811 ± 1.363
6.196AlaArg: 6.196 ± 0.735
7.362AlaSer: 7.362 ± 1.039
6.633AlaThr: 6.633 ± 0.987
7.216AlaVal: 7.216 ± 0.889
1.093AlaTrp: 1.093 ± 0.354
3.061AlaTyr: 3.061 ± 0.348
0.0AlaXaa: 0.0 ± 0.0
Cys
1.239CysAla: 1.239 ± 0.354
0.219CysCys: 0.219 ± 0.126
0.802CysAsp: 0.802 ± 0.216
0.875CysGlu: 0.875 ± 0.291
0.51CysPhe: 0.51 ± 0.191
0.875CysGly: 0.875 ± 0.311
0.364CysHis: 0.364 ± 0.196
0.219CysIle: 0.219 ± 0.104
0.51CysLys: 0.51 ± 0.201
0.583CysLeu: 0.583 ± 0.194
0.146CysMet: 0.146 ± 0.091
0.364CysAsn: 0.364 ± 0.157
0.583CysPro: 0.583 ± 0.202
0.292CysGln: 0.292 ± 0.127
1.02CysArg: 1.02 ± 0.336
0.364CysSer: 0.364 ± 0.161
0.219CysThr: 0.219 ± 0.125
0.802CysVal: 0.802 ± 0.244
0.0CysTrp: 0.0 ± 0.0
0.51CysTyr: 0.51 ± 0.244
0.0CysXaa: 0.0 ± 0.0
Asp
5.977AspAla: 5.977 ± 0.784
0.656AspCys: 0.656 ± 0.242
3.207AspAsp: 3.207 ± 0.419
4.738AspGlu: 4.738 ± 0.678
2.333AspPhe: 2.333 ± 0.396
5.03AspGly: 5.03 ± 0.817
0.583AspHis: 0.583 ± 0.203
2.551AspIle: 2.551 ± 0.434
2.187AspLys: 2.187 ± 0.447
4.228AspLeu: 4.228 ± 0.451
1.093AspMet: 1.093 ± 0.295
2.333AspAsn: 2.333 ± 0.311
2.478AspPro: 2.478 ± 0.368
1.166AspGln: 1.166 ± 0.354
2.624AspArg: 2.624 ± 0.512
2.333AspSer: 2.333 ± 0.39
2.624AspThr: 2.624 ± 0.43
2.916AspVal: 2.916 ± 0.385
0.729AspTrp: 0.729 ± 0.206
2.26AspTyr: 2.26 ± 0.431
0.0AspXaa: 0.0 ± 0.0
Glu
7.07GluAla: 7.07 ± 0.893
0.802GluCys: 0.802 ± 0.232
2.697GluAsp: 2.697 ± 0.4
3.863GluGlu: 3.863 ± 0.57
2.041GluPhe: 2.041 ± 0.336
4.155GluGly: 4.155 ± 0.565
0.802GluHis: 0.802 ± 0.226
3.207GluIle: 3.207 ± 0.356
2.843GluLys: 2.843 ± 0.663
5.977GluLeu: 5.977 ± 0.688
1.677GluMet: 1.677 ± 0.328
2.26GluAsn: 2.26 ± 0.319
2.405GluPro: 2.405 ± 0.563
2.843GluGln: 2.843 ± 0.381
3.863GluArg: 3.863 ± 0.473
2.916GluSer: 2.916 ± 0.425
3.353GluThr: 3.353 ± 0.366
5.321GluVal: 5.321 ± 0.558
1.458GluTrp: 1.458 ± 0.406
1.968GluTyr: 1.968 ± 0.375
0.0GluXaa: 0.0 ± 0.0
Phe
2.843PheAla: 2.843 ± 0.501
0.802PheCys: 0.802 ± 0.256
3.572PheAsp: 3.572 ± 0.503
2.478PheGlu: 2.478 ± 0.425
1.458PhePhe: 1.458 ± 0.331
3.134PheGly: 3.134 ± 0.313
0.219PheHis: 0.219 ± 0.143
2.187PheIle: 2.187 ± 0.442
1.531PheLys: 1.531 ± 0.268
1.604PheLeu: 1.604 ± 0.408
1.385PheMet: 1.385 ± 0.273
2.333PheAsn: 2.333 ± 0.494
1.531PhePro: 1.531 ± 0.418
1.239PheGln: 1.239 ± 0.307
2.041PheArg: 2.041 ± 0.328
1.604PheSer: 1.604 ± 0.334
2.551PheThr: 2.551 ± 0.428
3.499PheVal: 3.499 ± 0.47
0.51PheTrp: 0.51 ± 0.188
1.385PheTyr: 1.385 ± 0.327
0.0PheXaa: 0.0 ± 0.0
Gly
8.018GlyAla: 8.018 ± 1.053
0.875GlyCys: 0.875 ± 0.289
4.592GlyAsp: 4.592 ± 0.542
5.03GlyGlu: 5.03 ± 0.574
3.353GlyPhe: 3.353 ± 0.45
6.05GlyGly: 6.05 ± 0.977
1.02GlyHis: 1.02 ± 0.377
3.79GlyIle: 3.79 ± 0.458
4.738GlyLys: 4.738 ± 0.573
6.56GlyLeu: 6.56 ± 0.733
1.166GlyMet: 1.166 ± 0.34
2.551GlyAsn: 2.551 ± 0.53
3.207GlyPro: 3.207 ± 0.42
4.228GlyGln: 4.228 ± 0.857
2.77GlyArg: 2.77 ± 0.466
4.228GlySer: 4.228 ± 1.046
5.321GlyThr: 5.321 ± 0.659
5.977GlyVal: 5.977 ± 0.575
1.458GlyTrp: 1.458 ± 0.398
2.551GlyTyr: 2.551 ± 0.453
0.0GlyXaa: 0.0 ± 0.0
His
1.531HisAla: 1.531 ± 0.306
0.364HisCys: 0.364 ± 0.202
0.437HisAsp: 0.437 ± 0.16
0.583HisGlu: 0.583 ± 0.185
0.729HisPhe: 0.729 ± 0.224
0.948HisGly: 0.948 ± 0.224
0.073HisHis: 0.073 ± 0.082
0.875HisIle: 0.875 ± 0.271
0.656HisLys: 0.656 ± 0.184
1.239HisLeu: 1.239 ± 0.389
0.292HisMet: 0.292 ± 0.139
0.51HisAsn: 0.51 ± 0.181
0.656HisPro: 0.656 ± 0.224
0.073HisGln: 0.073 ± 0.075
1.02HisArg: 1.02 ± 0.337
0.875HisSer: 0.875 ± 0.233
0.583HisThr: 0.583 ± 0.241
1.458HisVal: 1.458 ± 0.372
0.073HisTrp: 0.073 ± 0.079
0.364HisTyr: 0.364 ± 0.125
0.0HisXaa: 0.0 ± 0.0
Ile
7.216IleAla: 7.216 ± 0.589
0.583IleCys: 0.583 ± 0.228
4.082IleAsp: 4.082 ± 0.511
4.301IleGlu: 4.301 ± 0.579
0.948IlePhe: 0.948 ± 0.322
3.936IleGly: 3.936 ± 0.38
0.729IleHis: 0.729 ± 0.209
2.916IleIle: 2.916 ± 0.495
2.989IleLys: 2.989 ± 0.536
2.916IleLeu: 2.916 ± 0.459
1.02IleMet: 1.02 ± 0.266
2.77IleAsn: 2.77 ± 0.453
2.478IlePro: 2.478 ± 0.438
1.385IleGln: 1.385 ± 0.271
2.333IleArg: 2.333 ± 0.391
3.134IleSer: 3.134 ± 0.497
3.572IleThr: 3.572 ± 0.466
4.009IleVal: 4.009 ± 0.563
0.51IleTrp: 0.51 ± 0.171
1.312IleTyr: 1.312 ± 0.371
0.0IleXaa: 0.0 ± 0.0
Lys
7.508LysAla: 7.508 ± 0.934
0.51LysCys: 0.51 ± 0.21
2.624LysAsp: 2.624 ± 0.523
2.697LysGlu: 2.697 ± 0.542
2.187LysPhe: 2.187 ± 0.296
2.989LysGly: 2.989 ± 0.431
1.02LysHis: 1.02 ± 0.3
2.624LysIle: 2.624 ± 0.346
4.301LysLys: 4.301 ± 0.824
5.54LysLeu: 5.54 ± 0.709
1.166LysMet: 1.166 ± 0.297
2.551LysAsn: 2.551 ± 0.499
2.26LysPro: 2.26 ± 0.502
1.312LysGln: 1.312 ± 0.279
2.916LysArg: 2.916 ± 0.445
3.645LysSer: 3.645 ± 0.491
3.572LysThr: 3.572 ± 0.466
4.155LysVal: 4.155 ± 0.583
0.729LysTrp: 0.729 ± 0.239
1.239LysTyr: 1.239 ± 0.255
0.0LysXaa: 0.0 ± 0.0
Leu
8.164LeuAla: 8.164 ± 1.077
0.364LeuCys: 0.364 ± 0.174
4.592LeuAsp: 4.592 ± 0.549
4.301LeuGlu: 4.301 ± 0.585
3.863LeuPhe: 3.863 ± 0.524
4.373LeuGly: 4.373 ± 0.568
0.875LeuHis: 0.875 ± 0.321
4.082LeuIle: 4.082 ± 0.476
3.79LeuLys: 3.79 ± 0.506
4.884LeuLeu: 4.884 ± 0.515
1.604LeuMet: 1.604 ± 0.343
4.155LeuAsn: 4.155 ± 0.643
4.738LeuPro: 4.738 ± 0.704
3.572LeuGln: 3.572 ± 0.824
4.228LeuArg: 4.228 ± 0.552
6.05LeuSer: 6.05 ± 0.549
4.446LeuThr: 4.446 ± 0.528
3.572LeuVal: 3.572 ± 0.5
1.239LeuTrp: 1.239 ± 0.279
2.77LeuTyr: 2.77 ± 0.518
0.0LeuXaa: 0.0 ± 0.0
Met
2.916MetAla: 2.916 ± 0.432
0.146MetCys: 0.146 ± 0.104
0.875MetAsp: 0.875 ± 0.225
0.583MetGlu: 0.583 ± 0.207
0.875MetPhe: 0.875 ± 0.251
1.749MetGly: 1.749 ± 0.312
0.364MetHis: 0.364 ± 0.188
0.948MetIle: 0.948 ± 0.299
1.531MetLys: 1.531 ± 0.392
2.114MetLeu: 2.114 ± 0.52
0.292MetMet: 0.292 ± 0.172
1.312MetAsn: 1.312 ± 0.283
1.968MetPro: 1.968 ± 0.391
0.875MetGln: 0.875 ± 0.329
1.093MetArg: 1.093 ± 0.264
1.312MetSer: 1.312 ± 0.372
1.677MetThr: 1.677 ± 0.269
0.802MetVal: 0.802 ± 0.282
0.292MetTrp: 0.292 ± 0.135
0.583MetTyr: 0.583 ± 0.203
0.0MetXaa: 0.0 ± 0.0
Asn
4.957AsnAla: 4.957 ± 0.817
0.583AsnCys: 0.583 ± 0.219
2.26AsnAsp: 2.26 ± 0.381
2.843AsnGlu: 2.843 ± 0.434
1.531AsnPhe: 1.531 ± 0.247
4.811AsnGly: 4.811 ± 0.66
0.437AsnHis: 0.437 ± 0.191
2.26AsnIle: 2.26 ± 0.424
2.478AsnLys: 2.478 ± 0.451
2.989AsnLeu: 2.989 ± 0.508
0.802AsnMet: 0.802 ± 0.238
2.114AsnAsn: 2.114 ± 0.59
3.353AsnPro: 3.353 ± 0.497
1.166AsnGln: 1.166 ± 0.25
2.697AsnArg: 2.697 ± 0.398
2.114AsnSer: 2.114 ± 0.347
2.989AsnThr: 2.989 ± 0.41
5.102AsnVal: 5.102 ± 0.573
0.583AsnTrp: 0.583 ± 0.193
1.02AsnTyr: 1.02 ± 0.326
0.0AsnXaa: 0.0 ± 0.0
Pro
5.102ProAla: 5.102 ± 0.907
0.364ProCys: 0.364 ± 0.149
2.551ProAsp: 2.551 ± 0.522
3.499ProGlu: 3.499 ± 0.523
1.604ProPhe: 1.604 ± 0.307
4.082ProGly: 4.082 ± 0.821
1.02ProHis: 1.02 ± 0.281
2.989ProIle: 2.989 ± 0.435
2.26ProLys: 2.26 ± 0.429
3.207ProLeu: 3.207 ± 0.596
0.875ProMet: 0.875 ± 0.224
3.499ProAsn: 3.499 ± 0.702
2.916ProPro: 2.916 ± 0.522
3.426ProGln: 3.426 ± 0.732
1.604ProArg: 1.604 ± 0.399
2.551ProSer: 2.551 ± 0.548
3.863ProThr: 3.863 ± 0.609
3.426ProVal: 3.426 ± 0.438
0.51ProTrp: 0.51 ± 0.203
1.749ProTyr: 1.749 ± 0.384
0.0ProXaa: 0.0 ± 0.0
Gln
4.155GlnAla: 4.155 ± 0.842
0.51GlnCys: 0.51 ± 0.206
1.239GlnAsp: 1.239 ± 0.306
1.312GlnGlu: 1.312 ± 0.359
0.802GlnPhe: 0.802 ± 0.236
3.061GlnGly: 3.061 ± 0.618
0.364GlnHis: 0.364 ± 0.183
2.187GlnIle: 2.187 ± 0.382
2.551GlnLys: 2.551 ± 0.487
4.811GlnLeu: 4.811 ± 0.772
1.02GlnMet: 1.02 ± 0.292
2.187GlnAsn: 2.187 ± 0.49
2.26GlnPro: 2.26 ± 0.8
4.155GlnGln: 4.155 ± 1.582
3.207GlnArg: 3.207 ± 0.875
2.478GlnSer: 2.478 ± 0.545
3.28GlnThr: 3.28 ± 0.766
1.968GlnVal: 1.968 ± 0.385
0.364GlnTrp: 0.364 ± 0.157
1.093GlnTyr: 1.093 ± 0.226
0.0GlnXaa: 0.0 ± 0.0
Arg
5.686ArgAla: 5.686 ± 0.99
0.292ArgCys: 0.292 ± 0.155
3.426ArgAsp: 3.426 ± 0.492
3.134ArgGlu: 3.134 ± 0.516
2.041ArgPhe: 2.041 ± 0.461
3.061ArgGly: 3.061 ± 0.481
0.656ArgHis: 0.656 ± 0.237
4.009ArgIle: 4.009 ± 0.563
2.989ArgLys: 2.989 ± 0.489
3.936ArgLeu: 3.936 ± 0.535
1.749ArgMet: 1.749 ± 0.304
1.531ArgAsn: 1.531 ± 0.297
2.551ArgPro: 2.551 ± 0.415
2.405ArgGln: 2.405 ± 0.355
3.134ArgArg: 3.134 ± 0.591
2.114ArgSer: 2.114 ± 0.336
2.916ArgThr: 2.916 ± 0.497
3.061ArgVal: 3.061 ± 0.526
1.093ArgTrp: 1.093 ± 0.243
2.26ArgTyr: 2.26 ± 0.394
0.0ArgXaa: 0.0 ± 0.0
Ser
5.831SerAla: 5.831 ± 0.782
0.437SerCys: 0.437 ± 0.157
3.353SerAsp: 3.353 ± 0.495
3.207SerGlu: 3.207 ± 0.539
2.405SerPhe: 2.405 ± 0.466
5.977SerGly: 5.977 ± 0.841
0.364SerHis: 0.364 ± 0.218
2.624SerIle: 2.624 ± 0.441
4.301SerLys: 4.301 ± 0.494
3.79SerLeu: 3.79 ± 0.455
1.02SerMet: 1.02 ± 0.269
2.041SerAsn: 2.041 ± 0.516
2.624SerPro: 2.624 ± 0.469
3.061SerGln: 3.061 ± 0.593
2.916SerArg: 2.916 ± 0.384
3.28SerSer: 3.28 ± 0.527
3.061SerThr: 3.061 ± 0.466
3.134SerVal: 3.134 ± 0.433
0.729SerTrp: 0.729 ± 0.193
2.041SerTyr: 2.041 ± 0.373
0.0SerXaa: 0.0 ± 0.0
Thr
6.706ThrAla: 6.706 ± 1.491
0.292ThrCys: 0.292 ± 0.15
2.405ThrAsp: 2.405 ± 0.38
4.519ThrGlu: 4.519 ± 0.675
2.405ThrPhe: 2.405 ± 0.464
6.05ThrGly: 6.05 ± 0.652
0.802ThrHis: 0.802 ± 0.244
2.624ThrIle: 2.624 ± 0.492
3.717ThrLys: 3.717 ± 0.563
5.831ThrLeu: 5.831 ± 0.669
1.312ThrMet: 1.312 ± 0.248
2.989ThrAsn: 2.989 ± 0.616
3.572ThrPro: 3.572 ± 0.491
2.187ThrGln: 2.187 ± 0.41
2.624ThrArg: 2.624 ± 0.461
2.843ThrSer: 2.843 ± 0.572
4.009ThrThr: 4.009 ± 0.679
4.155ThrVal: 4.155 ± 0.602
0.583ThrTrp: 0.583 ± 0.142
2.26ThrTyr: 2.26 ± 0.401
0.0ThrXaa: 0.0 ± 0.0
Val
8.674ValAla: 8.674 ± 0.728
0.948ValCys: 0.948 ± 0.284
2.405ValAsp: 2.405 ± 0.334
4.082ValGlu: 4.082 ± 0.65
3.499ValPhe: 3.499 ± 0.571
4.957ValGly: 4.957 ± 0.521
1.02ValHis: 1.02 ± 0.29
3.79ValIle: 3.79 ± 0.44
3.863ValLys: 3.863 ± 0.367
4.155ValLeu: 4.155 ± 0.479
1.895ValMet: 1.895 ± 0.362
2.624ValAsn: 2.624 ± 0.399
4.592ValPro: 4.592 ± 0.677
2.77ValGln: 2.77 ± 0.646
2.77ValArg: 2.77 ± 0.444
3.936ValSer: 3.936 ± 0.521
4.738ValThr: 4.738 ± 0.871
4.301ValVal: 4.301 ± 0.561
1.385ValTrp: 1.385 ± 0.356
2.333ValTyr: 2.333 ± 0.468
0.0ValXaa: 0.0 ± 0.0
Trp
1.166TrpAla: 1.166 ± 0.284
0.292TrpCys: 0.292 ± 0.183
0.437TrpAsp: 0.437 ± 0.208
0.437TrpGlu: 0.437 ± 0.166
0.802TrpPhe: 0.802 ± 0.226
0.875TrpGly: 0.875 ± 0.301
0.292TrpHis: 0.292 ± 0.141
0.729TrpIle: 0.729 ± 0.259
0.437TrpLys: 0.437 ± 0.192
1.093TrpLeu: 1.093 ± 0.255
0.364TrpMet: 0.364 ± 0.124
0.51TrpAsn: 0.51 ± 0.213
1.093TrpPro: 1.093 ± 0.292
0.583TrpGln: 0.583 ± 0.206
1.239TrpArg: 1.239 ± 0.296
0.583TrpSer: 0.583 ± 0.224
0.656TrpThr: 0.656 ± 0.268
1.166TrpVal: 1.166 ± 0.234
0.146TrpTrp: 0.146 ± 0.097
0.875TrpTyr: 0.875 ± 0.272
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.478TyrAla: 2.478 ± 0.448
0.656TyrCys: 0.656 ± 0.257
2.041TyrAsp: 2.041 ± 0.399
2.041TyrGlu: 2.041 ± 0.374
1.458TyrPhe: 1.458 ± 0.334
2.843TyrGly: 2.843 ± 0.554
0.51TyrHis: 0.51 ± 0.197
1.531TyrIle: 1.531 ± 0.273
1.166TyrLys: 1.166 ± 0.325
1.968TyrLeu: 1.968 ± 0.331
0.729TyrMet: 0.729 ± 0.303
2.333TyrAsn: 2.333 ± 0.44
1.385TyrPro: 1.385 ± 0.306
1.458TyrGln: 1.458 ± 0.408
1.749TyrArg: 1.749 ± 0.337
2.478TyrSer: 2.478 ± 0.467
1.822TyrThr: 1.822 ± 0.372
2.697TyrVal: 2.697 ± 0.43
0.292TyrTrp: 0.292 ± 0.135
1.239TyrTyr: 1.239 ± 0.454
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13720 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski