Amino acid dipepetide frequency for Citromicrobium phage vB_Cib_ssDNA_P1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.355AlaAla: 11.355 ± 3.369
2.271AlaCys: 2.271 ± 0.715
3.028AlaAsp: 3.028 ± 1.267
6.813AlaGlu: 6.813 ± 1.727
4.542AlaPhe: 4.542 ± 1.289
4.542AlaGly: 4.542 ± 1.446
2.271AlaHis: 2.271 ± 1.993
5.299AlaIle: 5.299 ± 1.616
5.299AlaLys: 5.299 ± 1.582
7.57AlaLeu: 7.57 ± 1.736
0.757AlaMet: 0.757 ± 0.627
5.299AlaAsn: 5.299 ± 1.307
8.327AlaPro: 8.327 ± 3.691
2.271AlaGln: 2.271 ± 1.061
7.57AlaArg: 7.57 ± 2.016
7.57AlaSer: 7.57 ± 1.817
3.785AlaThr: 3.785 ± 2.245
4.542AlaVal: 4.542 ± 3.905
1.514AlaTrp: 1.514 ± 0.591
2.271AlaTyr: 2.271 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
1.514CysAla: 1.514 ± 1.198
0.0CysCys: 0.0 ± 0.0
0.757CysAsp: 0.757 ± 0.599
0.0CysGlu: 0.0 ± 0.0
0.0CysPhe: 0.0 ± 0.0
2.271CysGly: 2.271 ± 1.764
0.757CysHis: 0.757 ± 0.899
0.757CysIle: 0.757 ± 0.899
0.757CysLys: 0.757 ± 0.664
1.514CysLeu: 1.514 ± 0.724
0.757CysMet: 0.757 ± 0.599
0.0CysAsn: 0.0 ± 0.0
1.514CysPro: 1.514 ± 0.716
0.0CysGln: 0.0 ± 0.0
0.757CysArg: 0.757 ± 0.664
1.514CysSer: 1.514 ± 0.724
0.0CysThr: 0.0 ± 0.0
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
9.841AspAla: 9.841 ± 2.148
0.757AspCys: 0.757 ± 0.599
1.514AspAsp: 1.514 ± 0.724
3.785AspGlu: 3.785 ± 2.343
3.028AspPhe: 3.028 ± 0.939
5.299AspGly: 5.299 ± 2.249
0.0AspHis: 0.0 ± 0.0
0.757AspIle: 0.757 ± 0.899
4.542AspLys: 4.542 ± 1.517
1.514AspLeu: 1.514 ± 1.254
3.028AspMet: 3.028 ± 0.863
0.757AspAsn: 0.757 ± 0.899
6.056AspPro: 6.056 ± 1.417
3.028AspGln: 3.028 ± 0.958
3.785AspArg: 3.785 ± 1.548
1.514AspSer: 1.514 ± 1.254
3.028AspThr: 3.028 ± 1.554
0.757AspVal: 0.757 ± 0.899
2.271AspTrp: 2.271 ± 1.881
3.028AspTyr: 3.028 ± 0.711
0.0AspXaa: 0.0 ± 0.0
Glu
4.542GluAla: 4.542 ± 1.54
0.757GluCys: 0.757 ± 0.664
4.542GluAsp: 4.542 ± 0.555
2.271GluGlu: 2.271 ± 1.602
0.0GluPhe: 0.0 ± 0.0
3.028GluGly: 3.028 ± 1.344
0.0GluHis: 0.0 ± 0.0
4.542GluIle: 4.542 ± 2.024
3.785GluLys: 3.785 ± 2.128
5.299GluLeu: 5.299 ± 1.088
1.514GluMet: 1.514 ± 0.591
1.514GluAsn: 1.514 ± 0.591
8.327GluPro: 8.327 ± 3.461
1.514GluGln: 1.514 ± 1.546
1.514GluArg: 1.514 ± 1.329
2.271GluSer: 2.271 ± 0.439
4.542GluThr: 4.542 ± 2.33
3.028GluVal: 3.028 ± 3.005
0.0GluTrp: 0.0 ± 0.0
1.514GluTyr: 1.514 ± 0.591
0.0GluXaa: 0.0 ± 0.0
Phe
0.757PheAla: 0.757 ± 0.627
0.0PheCys: 0.0 ± 0.0
0.757PheAsp: 0.757 ± 0.627
0.757PheGlu: 0.757 ± 0.627
1.514PhePhe: 1.514 ± 1.254
6.813PheGly: 6.813 ± 1.96
0.757PheHis: 0.757 ± 0.599
0.757PheIle: 0.757 ± 0.627
1.514PheLys: 1.514 ± 1.254
8.327PheLeu: 8.327 ± 2.183
0.757PheMet: 0.757 ± 0.599
1.514PheAsn: 1.514 ± 1.198
0.757PhePro: 0.757 ± 0.599
0.757PheGln: 0.757 ± 0.627
1.514PheArg: 1.514 ± 0.591
1.514PheSer: 1.514 ± 0.716
2.271PheThr: 2.271 ± 1.061
1.514PheVal: 1.514 ± 0.892
0.0PheTrp: 0.0 ± 0.0
2.271PheTyr: 2.271 ± 1.678
0.0PheXaa: 0.0 ± 0.0
Gly
7.57GlyAla: 7.57 ± 1.988
0.757GlyCys: 0.757 ± 0.899
6.056GlyAsp: 6.056 ± 2.1
4.542GlyGlu: 4.542 ± 0.555
3.028GlyPhe: 3.028 ± 0.761
5.299GlyGly: 5.299 ± 2.459
1.514GlyHis: 1.514 ± 0.716
6.813GlyIle: 6.813 ± 1.583
2.271GlyLys: 2.271 ± 1.576
4.542GlyLeu: 4.542 ± 1.083
3.785GlyMet: 3.785 ± 0.931
2.271GlyAsn: 2.271 ± 1.253
3.785GlyPro: 3.785 ± 1.575
5.299GlyGln: 5.299 ± 1.346
3.785GlyArg: 3.785 ± 2.083
4.542GlySer: 4.542 ± 2.123
6.813GlyThr: 6.813 ± 2.899
3.028GlyVal: 3.028 ± 0.939
0.757GlyTrp: 0.757 ± 0.627
2.271GlyTyr: 2.271 ± 1.253
0.0GlyXaa: 0.0 ± 0.0
His
2.271HisAla: 2.271 ± 1.061
0.0HisCys: 0.0 ± 0.0
0.0HisAsp: 0.0 ± 0.0
1.514HisGlu: 1.514 ± 1.329
0.0HisPhe: 0.0 ± 0.0
0.757HisGly: 0.757 ± 0.899
0.0HisHis: 0.0 ± 0.0
1.514HisIle: 1.514 ± 0.716
3.028HisLys: 3.028 ± 1.555
0.0HisLeu: 0.0 ± 0.0
0.757HisMet: 0.757 ± 0.664
0.757HisAsn: 0.757 ± 0.664
2.271HisPro: 2.271 ± 0.715
1.514HisGln: 1.514 ± 0.962
1.514HisArg: 1.514 ± 1.329
0.757HisSer: 0.757 ± 0.899
0.757HisThr: 0.757 ± 1.194
0.0HisVal: 0.0 ± 0.0
0.757HisTrp: 0.757 ± 0.599
0.0HisTyr: 0.0 ± 0.0
0.0HisXaa: 0.0 ± 0.0
Ile
6.813IleAla: 6.813 ± 3.165
0.0IleCys: 0.0 ± 0.0
3.785IleAsp: 3.785 ± 3.135
1.514IleGlu: 1.514 ± 1.546
2.271IlePhe: 2.271 ± 1.797
7.57IleGly: 7.57 ± 2.8
2.271IleHis: 2.271 ± 1.995
1.514IleIle: 1.514 ± 0.892
3.028IleLys: 3.028 ± 1.64
3.028IleLeu: 3.028 ± 1.555
2.271IleMet: 2.271 ± 0.439
3.785IleAsn: 3.785 ± 1.6
4.542IlePro: 4.542 ± 2.322
0.0IleGln: 0.0 ± 0.0
3.028IleArg: 3.028 ± 1.183
1.514IleSer: 1.514 ± 1.254
3.028IleThr: 3.028 ± 1.769
0.757IleVal: 0.757 ± 0.627
0.0IleTrp: 0.0 ± 0.0
0.757IleTyr: 0.757 ± 0.599
0.0IleXaa: 0.0 ± 0.0
Lys
2.271LysAla: 2.271 ± 1.589
0.757LysCys: 0.757 ± 0.664
3.785LysAsp: 3.785 ± 2.343
3.028LysGlu: 3.028 ± 1.183
1.514LysPhe: 1.514 ± 0.591
3.785LysGly: 3.785 ± 1.888
1.514LysHis: 1.514 ± 0.591
0.0LysIle: 0.0 ± 0.0
3.785LysLys: 3.785 ± 4.324
2.271LysLeu: 2.271 ± 1.337
1.514LysMet: 1.514 ± 0.716
3.785LysAsn: 3.785 ± 0.955
3.028LysPro: 3.028 ± 1.183
1.514LysGln: 1.514 ± 0.724
5.299LysArg: 5.299 ± 1.573
1.514LysSer: 1.514 ± 0.716
2.271LysThr: 2.271 ± 1.589
2.271LysVal: 2.271 ± 1.18
0.757LysTrp: 0.757 ± 0.664
1.514LysTyr: 1.514 ± 0.724
0.0LysXaa: 0.0 ± 0.0
Leu
8.327LeuAla: 8.327 ± 2.074
0.757LeuCys: 0.757 ± 0.899
5.299LeuAsp: 5.299 ± 1.663
3.785LeuGlu: 3.785 ± 1.2
2.271LeuPhe: 2.271 ± 1.061
6.056LeuGly: 6.056 ± 1.421
0.757LeuHis: 0.757 ± 1.194
2.271LeuIle: 2.271 ± 1.012
3.028LeuLys: 3.028 ± 0.711
3.028LeuLeu: 3.028 ± 1.086
5.299LeuMet: 5.299 ± 2.638
0.0LeuAsn: 0.0 ± 0.0
6.813LeuPro: 6.813 ± 1.825
1.514LeuGln: 1.514 ± 0.724
5.299LeuArg: 5.299 ± 1.088
3.785LeuSer: 3.785 ± 0.802
8.327LeuThr: 8.327 ± 1.853
4.542LeuVal: 4.542 ± 2.687
3.028LeuTrp: 3.028 ± 1.432
1.514LeuTyr: 1.514 ± 0.892
0.0LeuXaa: 0.0 ± 0.0
Met
4.542MetAla: 4.542 ± 1.42
0.757MetCys: 0.757 ± 0.627
2.271MetAsp: 2.271 ± 0.715
3.028MetGlu: 3.028 ± 1.301
1.514MetPhe: 1.514 ± 0.591
1.514MetGly: 1.514 ± 1.254
1.514MetHis: 1.514 ± 0.724
1.514MetIle: 1.514 ± 1.254
0.0MetLys: 0.0 ± 0.0
1.514MetLeu: 1.514 ± 1.329
0.0MetMet: 0.0 ± 0.0
0.757MetAsn: 0.757 ± 0.599
2.271MetPro: 2.271 ± 1.012
0.0MetGln: 0.0 ± 0.0
4.542MetArg: 4.542 ± 2.214
1.514MetSer: 1.514 ± 1.254
1.514MetThr: 1.514 ± 1.198
0.757MetVal: 0.757 ± 0.627
0.0MetTrp: 0.0 ± 0.0
2.271MetTyr: 2.271 ± 1.327
0.0MetXaa: 0.0 ± 0.0
Asn
3.785AsnAla: 3.785 ± 1.746
2.271AsnCys: 2.271 ± 1.141
0.757AsnAsp: 0.757 ± 0.599
1.514AsnGlu: 1.514 ± 0.716
0.757AsnPhe: 0.757 ± 0.899
0.757AsnGly: 0.757 ± 0.627
0.0AsnHis: 0.0 ± 0.0
3.785AsnIle: 3.785 ± 1.6
0.0AsnLys: 0.0 ± 0.0
3.028AsnLeu: 3.028 ± 1.64
3.028AsnMet: 3.028 ± 0.939
1.514AsnAsn: 1.514 ± 0.892
3.785AsnPro: 3.785 ± 1.379
0.0AsnGln: 0.0 ± 0.0
0.757AsnArg: 0.757 ± 0.599
3.785AsnSer: 3.785 ± 1.428
2.271AsnThr: 2.271 ± 1.061
2.271AsnVal: 2.271 ± 1.012
0.0AsnTrp: 0.0 ± 0.0
0.757AsnTyr: 0.757 ± 0.627
0.0AsnXaa: 0.0 ± 0.0
Pro
10.598ProAla: 10.598 ± 5.311
1.514ProCys: 1.514 ± 0.962
3.785ProAsp: 3.785 ± 1.49
8.327ProGlu: 8.327 ± 3.189
3.028ProPhe: 3.028 ± 1.64
4.542ProGly: 4.542 ± 0.555
0.757ProHis: 0.757 ± 0.664
2.271ProIle: 2.271 ± 0.715
2.271ProLys: 2.271 ± 1.881
6.056ProLeu: 6.056 ± 1.345
1.514ProMet: 1.514 ± 1.135
1.514ProAsn: 1.514 ± 0.591
8.327ProPro: 8.327 ± 4.404
1.514ProGln: 1.514 ± 0.724
3.785ProArg: 3.785 ± 2.363
6.813ProSer: 6.813 ± 1.727
3.028ProThr: 3.028 ± 1.64
6.813ProVal: 6.813 ± 2.045
2.271ProTrp: 2.271 ± 1.797
3.028ProTyr: 3.028 ± 2.396
0.0ProXaa: 0.0 ± 0.0
Gln
1.514GlnAla: 1.514 ± 1.184
0.0GlnCys: 0.0 ± 0.0
4.542GlnAsp: 4.542 ± 1.826
2.271GlnGlu: 2.271 ± 0.439
1.514GlnPhe: 1.514 ± 1.254
3.028GlnGly: 3.028 ± 1.183
0.757GlnHis: 0.757 ± 0.599
0.757GlnIle: 0.757 ± 0.627
0.0GlnLys: 0.0 ± 0.0
1.514GlnLeu: 1.514 ± 1.329
0.0GlnMet: 0.0 ± 0.0
3.028GlnAsn: 3.028 ± 1.64
2.271GlnPro: 2.271 ± 0.439
0.757GlnGln: 0.757 ± 0.664
2.271GlnArg: 2.271 ± 0.715
1.514GlnSer: 1.514 ± 1.184
1.514GlnThr: 1.514 ± 0.962
0.757GlnVal: 0.757 ± 1.194
2.271GlnTrp: 2.271 ± 1.244
1.514GlnTyr: 1.514 ± 0.591
0.0GlnXaa: 0.0 ± 0.0
Arg
6.813ArgAla: 6.813 ± 2.25
0.757ArgCys: 0.757 ± 0.664
5.299ArgAsp: 5.299 ± 2.182
1.514ArgGlu: 1.514 ± 0.716
0.757ArgPhe: 0.757 ± 0.599
3.785ArgGly: 3.785 ± 1.447
0.0ArgHis: 0.0 ± 0.0
4.542ArgIle: 4.542 ± 2.033
3.785ArgLys: 3.785 ± 1.782
5.299ArgLeu: 5.299 ± 0.765
0.757ArgMet: 0.757 ± 0.664
2.271ArgAsn: 2.271 ± 1.327
4.542ArgPro: 4.542 ± 1.528
3.785ArgGln: 3.785 ± 0.752
6.056ArgArg: 6.056 ± 2.863
5.299ArgSer: 5.299 ± 2.676
6.056ArgThr: 6.056 ± 0.848
7.57ArgVal: 7.57 ± 1.918
0.757ArgTrp: 0.757 ± 0.627
3.028ArgTyr: 3.028 ± 1.853
0.0ArgXaa: 0.0 ± 0.0
Ser
4.542SerAla: 4.542 ± 1.734
0.757SerCys: 0.757 ± 0.599
1.514SerAsp: 1.514 ± 0.591
3.028SerGlu: 3.028 ± 1.62
2.271SerPhe: 2.271 ± 1.253
8.327SerGly: 8.327 ± 3.031
0.757SerHis: 0.757 ± 0.627
3.028SerIle: 3.028 ± 1.746
1.514SerLys: 1.514 ± 1.236
6.056SerLeu: 6.056 ± 1.916
0.757SerMet: 0.757 ± 0.664
2.271SerAsn: 2.271 ± 1.061
1.514SerPro: 1.514 ± 0.591
1.514SerGln: 1.514 ± 0.716
8.327SerArg: 8.327 ± 3.423
4.542SerSer: 4.542 ± 1.772
1.514SerThr: 1.514 ± 0.591
1.514SerVal: 1.514 ± 0.591
1.514SerTrp: 1.514 ± 0.591
1.514SerTyr: 1.514 ± 1.254
0.0SerXaa: 0.0 ± 0.0
Thr
5.299ThrAla: 5.299 ± 1.915
0.0ThrCys: 0.0 ± 0.0
6.056ThrAsp: 6.056 ± 4.099
2.271ThrGlu: 2.271 ± 0.439
3.028ThrPhe: 3.028 ± 1.924
4.542ThrGly: 4.542 ± 2.123
2.271ThrHis: 2.271 ± 1.327
3.785ThrIle: 3.785 ± 1.2
3.028ThrLys: 3.028 ± 1.555
5.299ThrLeu: 5.299 ± 0.998
3.028ThrMet: 3.028 ± 1.123
2.271ThrAsn: 2.271 ± 1.555
5.299ThrPro: 5.299 ± 1.663
1.514ThrGln: 1.514 ± 0.892
3.028ThrArg: 3.028 ± 0.812
0.757ThrSer: 0.757 ± 0.599
1.514ThrThr: 1.514 ± 0.591
2.271ThrVal: 2.271 ± 1.109
1.514ThrTrp: 1.514 ± 0.724
1.514ThrTyr: 1.514 ± 1.254
0.0ThrXaa: 0.0 ± 0.0
Val
4.542ValAla: 4.542 ± 3.091
0.757ValCys: 0.757 ± 0.664
3.028ValAsp: 3.028 ± 1.448
2.271ValGlu: 2.271 ± 1.37
2.271ValPhe: 2.271 ± 1.061
3.028ValGly: 3.028 ± 1.64
2.271ValHis: 2.271 ± 1.696
3.785ValIle: 3.785 ± 1.888
0.757ValLys: 0.757 ± 0.627
2.271ValLeu: 2.271 ± 1.37
0.0ValMet: 0.0 ± 0.0
0.0ValAsn: 0.0 ± 0.0
4.542ValPro: 4.542 ± 2.549
3.028ValGln: 3.028 ± 1.746
5.299ValArg: 5.299 ± 2.405
3.028ValSer: 3.028 ± 1.279
3.028ValThr: 3.028 ± 1.853
4.542ValVal: 4.542 ± 3.32
1.514ValTrp: 1.514 ± 1.617
1.514ValTyr: 1.514 ± 0.724
0.0ValXaa: 0.0 ± 0.0
Trp
0.0TrpAla: 0.0 ± 0.0
0.0TrpCys: 0.0 ± 0.0
1.514TrpAsp: 1.514 ± 0.591
0.757TrpGlu: 0.757 ± 0.599
0.757TrpPhe: 0.757 ± 0.664
0.757TrpGly: 0.757 ± 0.599
0.0TrpHis: 0.0 ± 0.0
1.514TrpIle: 1.514 ± 0.591
3.028TrpLys: 3.028 ± 0.812
1.514TrpLeu: 1.514 ± 1.503
0.757TrpMet: 0.757 ± 0.627
0.757TrpAsn: 0.757 ± 0.599
0.0TrpPro: 0.0 ± 0.0
1.514TrpGln: 1.514 ± 0.591
3.028TrpArg: 3.028 ± 1.555
1.514TrpSer: 1.514 ± 0.591
1.514TrpThr: 1.514 ± 0.724
0.757TrpVal: 0.757 ± 0.627
0.757TrpTrp: 0.757 ± 0.599
0.757TrpTyr: 0.757 ± 0.599
0.0TrpXaa: 0.0 ± 0.0
Tyr
0.0TyrAla: 0.0 ± 0.0
0.0TyrCys: 0.0 ± 0.0
0.757TyrAsp: 0.757 ± 0.599
1.514TyrGlu: 1.514 ± 0.892
0.757TyrPhe: 0.757 ± 0.627
3.028TyrGly: 3.028 ± 0.812
0.0TyrHis: 0.0 ± 0.0
2.271TyrIle: 2.271 ± 1.327
0.757TyrLys: 0.757 ± 0.599
6.056TyrLeu: 6.056 ± 2.168
0.757TyrMet: 0.757 ± 0.627
0.757TyrAsn: 0.757 ± 0.599
3.785TyrPro: 3.785 ± 2.245
0.757TyrGln: 0.757 ± 0.599
1.514TyrArg: 1.514 ± 1.329
1.514TyrSer: 1.514 ± 1.799
1.514TyrThr: 1.514 ± 1.254
3.785TyrVal: 3.785 ± 1.382
1.514TyrTrp: 1.514 ± 0.591
0.757TyrTyr: 0.757 ± 0.627
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6 proteins (1322 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski