Amino acid dipepetide frequency for Sulfobacillus thermosulfidooxidans (strain DSM 9293 / VKM B-1269 / AT-1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.765AlaAla: 7.765 ± 0.096
0.667AlaCys: 0.667 ± 0.026
3.98AlaAsp: 3.98 ± 0.06
4.496AlaGlu: 4.496 ± 0.069
3.316AlaPhe: 3.316 ± 0.057
6.208AlaGly: 6.208 ± 0.087
2.571AlaHis: 2.571 ± 0.049
6.035AlaIle: 6.035 ± 0.085
3.366AlaLys: 3.366 ± 0.061
11.254AlaLeu: 11.254 ± 0.11
2.633AlaMet: 2.633 ± 0.05
2.535AlaAsn: 2.535 ± 0.051
3.722AlaPro: 3.722 ± 0.063
4.43AlaGln: 4.43 ± 0.071
4.912AlaArg: 4.912 ± 0.075
5.069AlaSer: 5.069 ± 0.074
4.669AlaThr: 4.669 ± 0.075
7.131AlaVal: 7.131 ± 0.094
1.729AlaTrp: 1.729 ± 0.042
2.585AlaTyr: 2.585 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.5CysAla: 0.5 ± 0.022
0.07CysCys: 0.07 ± 0.008
0.336CysAsp: 0.336 ± 0.018
0.37CysGlu: 0.37 ± 0.02
0.182CysPhe: 0.182 ± 0.013
0.715CysGly: 0.715 ± 0.03
0.302CysHis: 0.302 ± 0.019
0.23CysIle: 0.23 ± 0.016
0.095CysLys: 0.095 ± 0.01
0.602CysLeu: 0.602 ± 0.025
0.115CysMet: 0.115 ± 0.009
0.13CysAsn: 0.13 ± 0.009
0.556CysPro: 0.556 ± 0.026
0.393CysGln: 0.393 ± 0.021
0.412CysArg: 0.412 ± 0.021
0.373CysSer: 0.373 ± 0.019
0.334CysThr: 0.334 ± 0.019
0.439CysVal: 0.439 ± 0.02
0.123CysTrp: 0.123 ± 0.011
0.198CysTyr: 0.198 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
4.149AspAla: 4.149 ± 0.065
0.273AspCys: 0.273 ± 0.016
2.567AspAsp: 2.567 ± 0.066
2.91AspGlu: 2.91 ± 0.064
1.78AspPhe: 1.78 ± 0.047
3.322AspGly: 3.322 ± 0.076
1.657AspHis: 1.657 ± 0.045
3.106AspIle: 3.106 ± 0.058
1.497AspLys: 1.497 ± 0.041
5.202AspLeu: 5.202 ± 0.076
1.271AspMet: 1.271 ± 0.039
1.365AspAsn: 1.365 ± 0.034
3.114AspPro: 3.114 ± 0.059
2.29AspGln: 2.29 ± 0.045
2.974AspArg: 2.974 ± 0.05
2.194AspSer: 2.194 ± 0.044
2.398AspThr: 2.398 ± 0.055
4.001AspVal: 4.001 ± 0.06
0.923AspTrp: 0.923 ± 0.033
1.454AspTyr: 1.454 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
5.04GluAla: 5.04 ± 0.087
0.29GluCys: 0.29 ± 0.018
2.679GluAsp: 2.679 ± 0.066
3.435GluGlu: 3.435 ± 0.08
1.663GluPhe: 1.663 ± 0.044
3.353GluGly: 3.353 ± 0.058
1.522GluHis: 1.522 ± 0.04
3.285GluIle: 3.285 ± 0.063
2.177GluLys: 2.177 ± 0.045
5.014GluLeu: 5.014 ± 0.074
1.513GluMet: 1.513 ± 0.035
1.681GluAsn: 1.681 ± 0.043
2.387GluPro: 2.387 ± 0.053
2.641GluGln: 2.641 ± 0.046
3.696GluArg: 3.696 ± 0.069
2.858GluSer: 2.858 ± 0.054
2.863GluThr: 2.863 ± 0.061
3.554GluVal: 3.554 ± 0.06
1.043GluTrp: 1.043 ± 0.033
1.293GluTyr: 1.293 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.047PheAla: 3.047 ± 0.057
0.276PheCys: 0.276 ± 0.015
1.964PheAsp: 1.964 ± 0.036
1.64PheGlu: 1.64 ± 0.039
1.553PhePhe: 1.553 ± 0.045
3.128PheGly: 3.128 ± 0.055
1.153PheHis: 1.153 ± 0.035
2.08PheIle: 2.08 ± 0.058
0.831PheLys: 0.831 ± 0.026
3.985PheLeu: 3.985 ± 0.073
0.906PheMet: 0.906 ± 0.032
1.111PheAsn: 1.111 ± 0.038
1.924PhePro: 1.924 ± 0.04
1.431PheGln: 1.431 ± 0.035
2.125PheArg: 2.125 ± 0.05
2.58PheSer: 2.58 ± 0.054
1.944PheThr: 1.944 ± 0.046
3.047PheVal: 3.047 ± 0.061
0.921PheTrp: 0.921 ± 0.033
1.194PheTyr: 1.194 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
5.739GlyAla: 5.739 ± 0.083
0.633GlyCys: 0.633 ± 0.028
3.017GlyAsp: 3.017 ± 0.049
3.463GlyGlu: 3.463 ± 0.056
3.216GlyPhe: 3.216 ± 0.058
5.355GlyGly: 5.355 ± 0.088
2.375GlyHis: 2.375 ± 0.044
5.537GlyIle: 5.537 ± 0.071
2.496GlyLys: 2.496 ± 0.053
8.262GlyLeu: 8.262 ± 0.101
2.068GlyMet: 2.068 ± 0.047
2.056GlyAsn: 2.056 ± 0.045
3.519GlyPro: 3.519 ± 0.071
3.735GlyGln: 3.735 ± 0.066
4.102GlyArg: 4.102 ± 0.072
4.449GlySer: 4.449 ± 0.073
4.539GlyThr: 4.539 ± 0.071
5.347GlyVal: 5.347 ± 0.078
1.553GlyTrp: 1.553 ± 0.044
2.697GlyTyr: 2.697 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.403HisAla: 2.403 ± 0.056
0.25HisCys: 0.25 ± 0.016
1.604HisAsp: 1.604 ± 0.038
1.586HisGlu: 1.586 ± 0.044
1.16HisPhe: 1.16 ± 0.031
2.385HisGly: 2.385 ± 0.049
1.494HisHis: 1.494 ± 0.044
1.813HisIle: 1.813 ± 0.047
0.849HisLys: 0.849 ± 0.027
3.36HisLeu: 3.36 ± 0.059
0.732HisMet: 0.732 ± 0.025
0.902HisAsn: 0.902 ± 0.031
2.181HisPro: 2.181 ± 0.047
1.589HisGln: 1.589 ± 0.043
1.774HisArg: 1.774 ± 0.042
1.499HisSer: 1.499 ± 0.038
1.39HisThr: 1.39 ± 0.037
2.45HisVal: 2.45 ± 0.049
0.744HisTrp: 0.744 ± 0.028
1.061HisTyr: 1.061 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.097IleAla: 6.097 ± 0.084
0.362IleCys: 0.362 ± 0.018
3.196IleAsp: 3.196 ± 0.057
3.195IleGlu: 3.195 ± 0.059
2.347IlePhe: 2.347 ± 0.053
4.808IleGly: 4.808 ± 0.078
1.749IleHis: 1.749 ± 0.04
3.623IleIle: 3.623 ± 0.069
1.829IleLys: 1.829 ± 0.044
6.562IleLeu: 6.562 ± 0.092
1.448IleMet: 1.448 ± 0.038
1.808IleAsn: 1.808 ± 0.047
3.758IlePro: 3.758 ± 0.061
2.411IleGln: 2.411 ± 0.048
3.85IleArg: 3.85 ± 0.063
3.521IleSer: 3.521 ± 0.059
3.693IleThr: 3.693 ± 0.064
5.107IleVal: 5.107 ± 0.083
1.165IleTrp: 1.165 ± 0.034
1.552IleTyr: 1.552 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
3.267LysAla: 3.267 ± 0.058
0.135LysCys: 0.135 ± 0.011
1.848LysAsp: 1.848 ± 0.046
2.082LysGlu: 2.082 ± 0.052
0.831LysPhe: 0.831 ± 0.027
2.16LysGly: 2.16 ± 0.045
0.786LysHis: 0.786 ± 0.027
2.07LysIle: 2.07 ± 0.05
1.484LysLys: 1.484 ± 0.046
2.642LysLeu: 2.642 ± 0.053
0.946LysMet: 0.946 ± 0.032
1.199LysAsn: 1.199 ± 0.036
1.945LysPro: 1.945 ± 0.038
1.31LysGln: 1.31 ± 0.033
2.181LysArg: 2.181 ± 0.05
2.068LysSer: 2.068 ± 0.046
2.544LysThr: 2.544 ± 0.049
2.284LysVal: 2.284 ± 0.052
0.663LysTrp: 0.663 ± 0.026
0.773LysTyr: 0.773 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
10.844LeuAla: 10.844 ± 0.117
0.646LeuCys: 0.646 ± 0.029
5.141LeuAsp: 5.141 ± 0.08
5.318LeuGlu: 5.318 ± 0.071
3.928LeuPhe: 3.928 ± 0.08
8.361LeuGly: 8.361 ± 0.108
2.753LeuHis: 2.753 ± 0.055
6.373LeuIle: 6.373 ± 0.089
3.92LeuLys: 3.92 ± 0.063
10.966LeuLeu: 10.966 ± 0.151
2.762LeuMet: 2.762 ± 0.062
3.266LeuAsn: 3.266 ± 0.056
5.72LeuPro: 5.72 ± 0.069
4.09LeuGln: 4.09 ± 0.061
5.887LeuArg: 5.887 ± 0.089
7.755LeuSer: 7.755 ± 0.089
6.93LeuThr: 6.93 ± 0.081
7.695LeuVal: 7.695 ± 0.078
2.516LeuTrp: 2.516 ± 0.054
2.857LeuTyr: 2.857 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
3.302MetAla: 3.302 ± 0.053
0.12MetCys: 0.12 ± 0.014
1.267MetAsp: 1.267 ± 0.035
1.263MetGlu: 1.263 ± 0.04
0.683MetPhe: 0.683 ± 0.029
2.146MetGly: 2.146 ± 0.051
0.586MetHis: 0.586 ± 0.023
1.589MetIle: 1.589 ± 0.043
0.996MetLys: 0.996 ± 0.03
2.22MetLeu: 2.22 ± 0.045
0.817MetMet: 0.817 ± 0.031
0.903MetAsn: 0.903 ± 0.029
1.428MetPro: 1.428 ± 0.036
0.919MetGln: 0.919 ± 0.03
1.51MetArg: 1.51 ± 0.036
1.553MetSer: 1.553 ± 0.038
1.967MetThr: 1.967 ± 0.042
2.164MetVal: 2.164 ± 0.043
0.362MetTrp: 0.362 ± 0.019
0.427MetTyr: 0.427 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.738AsnAla: 2.738 ± 0.049
0.145AsnCys: 0.145 ± 0.012
1.246AsnAsp: 1.246 ± 0.036
1.264AsnGlu: 1.264 ± 0.031
1.011AsnPhe: 1.011 ± 0.033
2.26AsnGly: 2.26 ± 0.063
1.031AsnHis: 1.031 ± 0.032
1.682AsnIle: 1.682 ± 0.045
0.827AsnLys: 0.827 ± 0.03
3.055AsnLeu: 3.055 ± 0.05
0.725AsnMet: 0.725 ± 0.029
0.913AsnAsn: 0.913 ± 0.035
2.494AsnPro: 2.494 ± 0.056
1.621AsnGln: 1.621 ± 0.051
1.873AsnArg: 1.873 ± 0.038
1.473AsnSer: 1.473 ± 0.044
1.618AsnThr: 1.618 ± 0.047
2.091AsnVal: 2.091 ± 0.05
0.588AsnTrp: 0.588 ± 0.024
0.792AsnTyr: 0.792 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
4.122ProAla: 4.122 ± 0.066
0.27ProCys: 0.27 ± 0.016
3.05ProAsp: 3.05 ± 0.053
3.4ProGlu: 3.4 ± 0.066
2.091ProPhe: 2.091 ± 0.045
4.116ProGly: 4.116 ± 0.072
1.824ProHis: 1.824 ± 0.042
2.965ProIle: 2.965 ± 0.049
2.031ProLys: 2.031 ± 0.039
5.835ProLeu: 5.835 ± 0.073
1.194ProMet: 1.194 ± 0.036
1.687ProAsn: 1.687 ± 0.047
2.619ProPro: 2.619 ± 0.051
2.38ProGln: 2.38 ± 0.049
2.66ProArg: 2.66 ± 0.051
3.756ProSer: 3.756 ± 0.066
2.991ProThr: 2.991 ± 0.055
4.641ProVal: 4.641 ± 0.067
1.269ProTrp: 1.269 ± 0.032
1.773ProTyr: 1.773 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
4.765GlnAla: 4.765 ± 0.074
0.315GlnCys: 0.315 ± 0.02
2.355GlnAsp: 2.355 ± 0.054
2.843GlnGlu: 2.843 ± 0.062
1.652GlnPhe: 1.652 ± 0.037
3.657GlnGly: 3.657 ± 0.063
1.419GlnHis: 1.419 ± 0.035
2.835GlnIle: 2.835 ± 0.057
1.769GlnLys: 1.769 ± 0.043
4.422GlnLeu: 4.422 ± 0.064
1.131GlnMet: 1.131 ± 0.032
1.474GlnAsn: 1.474 ± 0.044
1.867GlnPro: 1.867 ± 0.049
2.531GlnGln: 2.531 ± 0.055
2.633GlnArg: 2.633 ± 0.054
2.54GlnSer: 2.54 ± 0.048
2.329GlnThr: 2.329 ± 0.045
3.239GlnVal: 3.239 ± 0.054
1.254GlnTrp: 1.254 ± 0.035
1.433GlnTyr: 1.433 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
4.458ArgAla: 4.458 ± 0.072
0.403ArgCys: 0.403 ± 0.022
2.924ArgAsp: 2.924 ± 0.063
3.373ArgGlu: 3.373 ± 0.069
2.313ArgPhe: 2.313 ± 0.047
3.517ArgGly: 3.517 ± 0.065
2.184ArgHis: 2.184 ± 0.04
3.942ArgIle: 3.942 ± 0.069
1.914ArgLys: 1.914 ± 0.043
6.724ArgLeu: 6.724 ± 0.094
1.562ArgMet: 1.562 ± 0.04
1.533ArgAsn: 1.533 ± 0.035
2.897ArgPro: 2.897 ± 0.056
3.485ArgGln: 3.485 ± 0.049
4.171ArgArg: 4.171 ± 0.079
2.904ArgSer: 2.904 ± 0.058
2.884ArgThr: 2.884 ± 0.052
4.297ArgVal: 4.297 ± 0.071
1.22ArgTrp: 1.22 ± 0.036
2.172ArgTyr: 2.172 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
4.855SerAla: 4.855 ± 0.063
0.412SerCys: 0.412 ± 0.022
2.392SerAsp: 2.392 ± 0.055
2.539SerGlu: 2.539 ± 0.047
2.18SerPhe: 2.18 ± 0.047
4.668SerGly: 4.668 ± 0.086
2.101SerHis: 2.101 ± 0.046
3.059SerIle: 3.059 ± 0.059
1.533SerLys: 1.533 ± 0.032
7.098SerLeu: 7.098 ± 0.092
1.621SerMet: 1.621 ± 0.041
1.431SerAsn: 1.431 ± 0.037
3.772SerPro: 3.772 ± 0.072
3.154SerGln: 3.154 ± 0.064
3.623SerArg: 3.623 ± 0.056
4.409SerSer: 4.409 ± 0.104
3.224SerThr: 3.224 ± 0.059
4.364SerVal: 4.364 ± 0.068
1.346SerTrp: 1.346 ± 0.032
1.685SerTyr: 1.685 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
4.995ThrAla: 4.995 ± 0.088
0.356ThrCys: 0.356 ± 0.02
2.417ThrAsp: 2.417 ± 0.052
2.563ThrGlu: 2.563 ± 0.052
2.069ThrPhe: 2.069 ± 0.047
4.551ThrGly: 4.551 ± 0.066
1.707ThrHis: 1.707 ± 0.041
3.61ThrIle: 3.61 ± 0.056
1.658ThrLys: 1.658 ± 0.045
6.589ThrLeu: 6.589 ± 0.092
1.384ThrMet: 1.384 ± 0.04
1.494ThrAsn: 1.494 ± 0.041
3.594ThrPro: 3.594 ± 0.068
2.442ThrGln: 2.442 ± 0.048
3.058ThrArg: 3.058 ± 0.055
3.146ThrSer: 3.146 ± 0.073
3.468ThrThr: 3.468 ± 0.081
4.92ThrVal: 4.92 ± 0.075
1.228ThrTrp: 1.228 ± 0.039
1.585ThrTyr: 1.585 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
6.735ValAla: 6.735 ± 0.094
0.493ValCys: 0.493 ± 0.027
3.851ValAsp: 3.851 ± 0.06
3.927ValGlu: 3.927 ± 0.065
2.935ValPhe: 2.935 ± 0.046
5.567ValGly: 5.567 ± 0.084
2.055ValHis: 2.055 ± 0.051
5.402ValIle: 5.402 ± 0.075
2.693ValLys: 2.693 ± 0.058
8.371ValLeu: 8.371 ± 0.091
2.309ValMet: 2.309 ± 0.047
2.428ValAsn: 2.428 ± 0.05
4.237ValPro: 4.237 ± 0.062
2.729ValGln: 2.729 ± 0.05
4.085ValArg: 4.085 ± 0.07
4.752ValSer: 4.752 ± 0.074
4.422ValThr: 4.422 ± 0.069
6.54ValVal: 6.54 ± 0.084
1.144ValTrp: 1.144 ± 0.039
2.092ValTyr: 2.092 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
1.779TrpAla: 1.779 ± 0.041
0.123TrpCys: 0.123 ± 0.012
1.062TrpAsp: 1.062 ± 0.037
0.954TrpGlu: 0.954 ± 0.03
0.735TrpPhe: 0.735 ± 0.032
1.434TrpGly: 1.434 ± 0.042
0.741TrpHis: 0.741 ± 0.026
1.52TrpIle: 1.52 ± 0.041
0.622TrpLys: 0.622 ± 0.024
2.326TrpLeu: 2.326 ± 0.052
0.563TrpMet: 0.563 ± 0.024
0.707TrpAsn: 0.707 ± 0.026
1.141TrpPro: 1.141 ± 0.035
1.188TrpGln: 1.188 ± 0.034
1.271TrpArg: 1.271 ± 0.036
1.119TrpSer: 1.119 ± 0.035
1.26TrpThr: 1.26 ± 0.04
1.419TrpVal: 1.419 ± 0.04
0.499TrpTrp: 0.499 ± 0.022
0.493TrpTyr: 0.493 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.474TyrAla: 2.474 ± 0.046
0.236TyrCys: 0.236 ± 0.015
1.551TyrAsp: 1.551 ± 0.042
1.366TyrGlu: 1.366 ± 0.035
1.196TyrPhe: 1.196 ± 0.03
2.448TyrGly: 2.448 ± 0.043
1.214TyrHis: 1.214 ± 0.035
1.326TyrIle: 1.326 ± 0.038
0.629TyrLys: 0.629 ± 0.027
3.238TyrLeu: 3.238 ± 0.063
0.519TyrMet: 0.519 ± 0.025
0.822TyrAsn: 0.822 ± 0.03
1.802TyrPro: 1.802 ± 0.043
1.695TyrGln: 1.695 ± 0.041
2.079TyrArg: 2.079 ± 0.046
1.452TyrSer: 1.452 ± 0.041
1.367TyrThr: 1.367 ± 0.037
2.037TyrVal: 2.037 ± 0.04
0.666TyrTrp: 0.666 ± 0.027
0.95TyrTyr: 0.95 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3615 proteins (1062604 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski