Amino acid dipepetide frequency for EBPR podovirus 2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.972AlaAla: 16.972 ± 1.549
0.872AlaCys: 0.872 ± 0.282
8.169AlaAsp: 8.169 ± 0.805
9.2AlaGlu: 9.2 ± 1.182
3.172AlaPhe: 3.172 ± 0.434
11.182AlaGly: 11.182 ± 1.257
2.062AlaHis: 2.062 ± 0.447
6.107AlaIle: 6.107 ± 0.852
4.917AlaLys: 4.917 ± 0.701
9.993AlaLeu: 9.993 ± 0.936
5.234AlaMet: 5.234 ± 0.658
4.441AlaAsn: 4.441 ± 0.492
5.948AlaPro: 5.948 ± 0.706
4.759AlaGln: 4.759 ± 0.827
10.231AlaArg: 10.231 ± 1.03
6.662AlaSer: 6.662 ± 0.741
6.979AlaThr: 6.979 ± 0.918
9.596AlaVal: 9.596 ± 1.036
2.141AlaTrp: 2.141 ± 0.425
3.093AlaTyr: 3.093 ± 0.492
0.0AlaXaa: 0.0 ± 0.0
Cys
0.555CysAla: 0.555 ± 0.169
0.159CysCys: 0.159 ± 0.118
1.031CysAsp: 1.031 ± 0.338
1.031CysGlu: 1.031 ± 0.317
0.079CysPhe: 0.079 ± 0.071
1.031CysGly: 1.031 ± 0.383
0.159CysHis: 0.159 ± 0.113
0.714CysIle: 0.714 ± 0.238
0.159CysLys: 0.159 ± 0.118
0.714CysLeu: 0.714 ± 0.237
0.079CysMet: 0.079 ± 0.092
0.0CysAsn: 0.0 ± 0.0
0.476CysPro: 0.476 ± 0.203
0.476CysGln: 0.476 ± 0.228
0.793CysArg: 0.793 ± 0.232
0.476CysSer: 0.476 ± 0.159
0.793CysThr: 0.793 ± 0.306
1.031CysVal: 1.031 ± 0.358
0.159CysTrp: 0.159 ± 0.106
0.159CysTyr: 0.159 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
9.358AspAla: 9.358 ± 0.899
0.634AspCys: 0.634 ± 0.222
4.124AspAsp: 4.124 ± 0.636
3.886AspGlu: 3.886 ± 0.567
1.665AspPhe: 1.665 ± 0.325
6.9AspGly: 6.9 ± 0.791
2.062AspHis: 2.062 ± 0.467
2.062AspIle: 2.062 ± 0.442
1.903AspLys: 1.903 ± 0.357
6.107AspLeu: 6.107 ± 0.916
2.062AspMet: 2.062 ± 0.499
1.983AspAsn: 1.983 ± 0.288
4.283AspPro: 4.283 ± 0.747
1.983AspGln: 1.983 ± 0.423
4.045AspArg: 4.045 ± 0.668
1.983AspSer: 1.983 ± 0.351
3.648AspThr: 3.648 ± 0.548
2.855AspVal: 2.855 ± 0.479
1.348AspTrp: 1.348 ± 0.358
1.983AspTyr: 1.983 ± 0.38
0.0AspXaa: 0.0 ± 0.0
Glu
11.262GluAla: 11.262 ± 1.17
0.397GluCys: 0.397 ± 0.181
2.379GluAsp: 2.379 ± 0.479
2.379GluGlu: 2.379 ± 0.539
1.824GluPhe: 1.824 ± 0.379
4.283GluGly: 4.283 ± 0.664
0.555GluHis: 0.555 ± 0.171
3.331GluIle: 3.331 ± 0.547
3.014GluLys: 3.014 ± 0.515
3.49GluLeu: 3.49 ± 0.455
1.745GluMet: 1.745 ± 0.282
2.062GluAsn: 2.062 ± 0.333
2.3GluPro: 2.3 ± 0.56
2.459GluGln: 2.459 ± 0.599
4.759GluArg: 4.759 ± 0.775
2.459GluSer: 2.459 ± 0.376
2.934GluThr: 2.934 ± 0.575
3.49GluVal: 3.49 ± 0.518
1.586GluTrp: 1.586 ± 0.34
1.428GluTyr: 1.428 ± 0.332
0.0GluXaa: 0.0 ± 0.0
Phe
3.648PheAla: 3.648 ± 0.502
0.476PheCys: 0.476 ± 0.219
1.983PheAsp: 1.983 ± 0.404
0.952PheGlu: 0.952 ± 0.263
1.11PhePhe: 1.11 ± 0.338
3.331PheGly: 3.331 ± 0.557
0.634PheHis: 0.634 ± 0.219
0.952PheIle: 0.952 ± 0.335
1.11PheLys: 1.11 ± 0.29
1.745PheLeu: 1.745 ± 0.317
0.714PheMet: 0.714 ± 0.262
1.11PheAsn: 1.11 ± 0.241
1.428PhePro: 1.428 ± 0.336
0.476PheGln: 0.476 ± 0.159
2.221PheArg: 2.221 ± 0.359
1.983PheSer: 1.983 ± 0.397
1.507PheThr: 1.507 ± 0.414
2.459PheVal: 2.459 ± 0.544
0.317PheTrp: 0.317 ± 0.152
0.317PheTyr: 0.317 ± 0.13
0.0PheXaa: 0.0 ± 0.0
Gly
11.103GlyAla: 11.103 ± 1.552
0.634GlyCys: 0.634 ± 0.254
5.71GlyAsp: 5.71 ± 0.535
3.807GlyGlu: 3.807 ± 0.567
3.41GlyPhe: 3.41 ± 0.496
7.852GlyGly: 7.852 ± 1.328
1.586GlyHis: 1.586 ± 0.33
4.759GlyIle: 4.759 ± 0.622
3.965GlyLys: 3.965 ± 0.566
6.503GlyLeu: 6.503 ± 0.731
2.776GlyMet: 2.776 ± 0.432
2.855GlyAsn: 2.855 ± 0.596
3.093GlyPro: 3.093 ± 0.429
4.124GlyGln: 4.124 ± 0.831
5.234GlyArg: 5.234 ± 0.839
4.838GlySer: 4.838 ± 0.744
5.393GlyThr: 5.393 ± 1.03
5.948GlyVal: 5.948 ± 0.821
2.3GlyTrp: 2.3 ± 0.558
2.062GlyTyr: 2.062 ± 0.373
0.0GlyXaa: 0.0 ± 0.0
His
1.665HisAla: 1.665 ± 0.434
0.397HisCys: 0.397 ± 0.21
1.507HisAsp: 1.507 ± 0.407
0.555HisGlu: 0.555 ± 0.2
0.634HisPhe: 0.634 ± 0.225
1.586HisGly: 1.586 ± 0.282
0.397HisHis: 0.397 ± 0.245
0.872HisIle: 0.872 ± 0.27
0.555HisLys: 0.555 ± 0.213
0.872HisLeu: 0.872 ± 0.299
0.952HisMet: 0.952 ± 0.276
0.476HisAsn: 0.476 ± 0.202
1.428HisPro: 1.428 ± 0.369
0.634HisGln: 0.634 ± 0.246
1.586HisArg: 1.586 ± 0.383
1.11HisSer: 1.11 ± 0.298
0.714HisThr: 0.714 ± 0.284
0.952HisVal: 0.952 ± 0.262
0.159HisTrp: 0.159 ± 0.112
0.793HisTyr: 0.793 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
6.424IleAla: 6.424 ± 0.686
0.397IleCys: 0.397 ± 0.26
2.379IleAsp: 2.379 ± 0.416
4.441IleGlu: 4.441 ± 0.439
0.714IlePhe: 0.714 ± 0.257
4.441IleGly: 4.441 ± 0.68
0.397IleHis: 0.397 ± 0.173
1.269IleIle: 1.269 ± 0.247
2.062IleLys: 2.062 ± 0.278
3.41IleLeu: 3.41 ± 0.555
0.634IleMet: 0.634 ± 0.216
0.952IleAsn: 0.952 ± 0.23
2.379IlePro: 2.379 ± 0.37
1.19IleGln: 1.19 ± 0.289
3.252IleArg: 3.252 ± 0.425
2.538IleSer: 2.538 ± 0.394
2.934IleThr: 2.934 ± 0.504
2.617IleVal: 2.617 ± 0.435
1.031IleTrp: 1.031 ± 0.307
0.872IleTyr: 0.872 ± 0.299
0.0IleXaa: 0.0 ± 0.0
Lys
6.107LysAla: 6.107 ± 0.837
0.634LysCys: 0.634 ± 0.216
3.093LysAsp: 3.093 ± 0.474
1.586LysGlu: 1.586 ± 0.436
1.031LysPhe: 1.031 ± 0.312
3.49LysGly: 3.49 ± 0.522
1.031LysHis: 1.031 ± 0.278
0.793LysIle: 0.793 ± 0.279
1.428LysLys: 1.428 ± 0.31
3.014LysLeu: 3.014 ± 0.552
1.11LysMet: 1.11 ± 0.403
1.031LysAsn: 1.031 ± 0.253
2.855LysPro: 2.855 ± 0.447
1.19LysGln: 1.19 ± 0.316
2.459LysArg: 2.459 ± 0.477
2.379LysSer: 2.379 ± 0.49
1.824LysThr: 1.824 ± 0.364
2.3LysVal: 2.3 ± 0.451
0.555LysTrp: 0.555 ± 0.193
0.872LysTyr: 0.872 ± 0.358
0.0LysXaa: 0.0 ± 0.0
Leu
10.865LeuAla: 10.865 ± 0.852
0.952LeuCys: 0.952 ± 0.312
4.6LeuAsp: 4.6 ± 0.677
3.569LeuGlu: 3.569 ± 0.421
2.3LeuPhe: 2.3 ± 0.458
5.552LeuGly: 5.552 ± 0.789
1.665LeuHis: 1.665 ± 0.44
3.014LeuIle: 3.014 ± 0.518
2.934LeuLys: 2.934 ± 0.474
5.393LeuLeu: 5.393 ± 0.682
1.507LeuMet: 1.507 ± 0.347
2.141LeuAsn: 2.141 ± 0.419
4.996LeuPro: 4.996 ± 0.7
2.934LeuGln: 2.934 ± 0.514
7.217LeuArg: 7.217 ± 0.844
4.679LeuSer: 4.679 ± 0.611
4.521LeuThr: 4.521 ± 0.647
3.41LeuVal: 3.41 ± 0.479
1.19LeuTrp: 1.19 ± 0.329
1.824LeuTyr: 1.824 ± 0.464
0.0LeuXaa: 0.0 ± 0.0
Met
3.014MetAla: 3.014 ± 0.523
0.079MetCys: 0.079 ± 0.071
2.141MetAsp: 2.141 ± 0.45
1.428MetGlu: 1.428 ± 0.324
0.634MetPhe: 0.634 ± 0.247
2.459MetGly: 2.459 ± 0.374
0.476MetHis: 0.476 ± 0.182
1.269MetIle: 1.269 ± 0.3
1.586MetLys: 1.586 ± 0.334
1.745MetLeu: 1.745 ± 0.326
0.476MetMet: 0.476 ± 0.191
0.952MetAsn: 0.952 ± 0.26
1.665MetPro: 1.665 ± 0.42
2.062MetGln: 2.062 ± 0.643
2.617MetArg: 2.617 ± 0.431
1.507MetSer: 1.507 ± 0.329
2.934MetThr: 2.934 ± 0.468
1.348MetVal: 1.348 ± 0.409
0.397MetTrp: 0.397 ± 0.191
0.159MetTyr: 0.159 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
3.727AsnAla: 3.727 ± 0.678
0.238AsnCys: 0.238 ± 0.142
1.745AsnAsp: 1.745 ± 0.399
1.348AsnGlu: 1.348 ± 0.303
0.634AsnPhe: 0.634 ± 0.212
3.569AsnGly: 3.569 ± 0.532
0.238AsnHis: 0.238 ± 0.145
1.507AsnIle: 1.507 ± 0.374
0.634AsnLys: 0.634 ± 0.223
2.221AsnLeu: 2.221 ± 0.486
0.714AsnMet: 0.714 ± 0.218
0.872AsnAsn: 0.872 ± 0.246
3.014AsnPro: 3.014 ± 0.526
1.348AsnGln: 1.348 ± 0.42
2.221AsnArg: 2.221 ± 0.515
1.348AsnSer: 1.348 ± 0.327
1.348AsnThr: 1.348 ± 0.297
1.269AsnVal: 1.269 ± 0.277
1.031AsnTrp: 1.031 ± 0.3
0.952AsnTyr: 0.952 ± 0.31
0.0AsnXaa: 0.0 ± 0.0
Pro
5.948ProAla: 5.948 ± 0.65
0.476ProCys: 0.476 ± 0.208
4.679ProAsp: 4.679 ± 0.632
4.124ProGlu: 4.124 ± 0.694
1.745ProPhe: 1.745 ± 0.358
4.203ProGly: 4.203 ± 0.626
0.872ProHis: 0.872 ± 0.247
2.3ProIle: 2.3 ± 0.522
2.3ProLys: 2.3 ± 0.429
3.331ProLeu: 3.331 ± 0.494
1.428ProMet: 1.428 ± 0.441
1.507ProAsn: 1.507 ± 0.286
3.49ProPro: 3.49 ± 0.569
2.221ProGln: 2.221 ± 0.494
3.569ProArg: 3.569 ± 0.469
4.203ProSer: 4.203 ± 0.746
2.617ProThr: 2.617 ± 0.376
3.093ProVal: 3.093 ± 0.563
1.11ProTrp: 1.11 ± 0.316
0.793ProTyr: 0.793 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
5.314GlnAla: 5.314 ± 0.776
0.238GlnCys: 0.238 ± 0.146
2.379GlnAsp: 2.379 ± 0.461
2.221GlnGlu: 2.221 ± 0.426
0.793GlnPhe: 0.793 ± 0.239
3.014GlnGly: 3.014 ± 0.496
0.397GlnHis: 0.397 ± 0.196
1.983GlnIle: 1.983 ± 0.393
1.269GlnLys: 1.269 ± 0.264
2.221GlnLeu: 2.221 ± 0.461
1.745GlnMet: 1.745 ± 0.386
1.269GlnAsn: 1.269 ± 0.355
2.3GlnPro: 2.3 ± 0.414
1.983GlnGln: 1.983 ± 0.372
2.855GlnArg: 2.855 ± 0.544
1.665GlnSer: 1.665 ± 0.309
3.252GlnThr: 3.252 ± 0.565
2.538GlnVal: 2.538 ± 0.511
0.634GlnTrp: 0.634 ± 0.268
0.793GlnTyr: 0.793 ± 0.23
0.0GlnXaa: 0.0 ± 0.0
Arg
9.041ArgAla: 9.041 ± 0.844
1.428ArgCys: 1.428 ± 0.385
4.441ArgAsp: 4.441 ± 0.657
5.552ArgGlu: 5.552 ± 0.69
2.379ArgPhe: 2.379 ± 0.475
5.234ArgGly: 5.234 ± 0.67
1.428ArgHis: 1.428 ± 0.35
3.965ArgIle: 3.965 ± 0.648
3.727ArgLys: 3.727 ± 0.504
7.058ArgLeu: 7.058 ± 0.869
1.507ArgMet: 1.507 ± 0.357
2.141ArgAsn: 2.141 ± 0.486
2.855ArgPro: 2.855 ± 0.566
3.252ArgGln: 3.252 ± 0.469
5.948ArgArg: 5.948 ± 0.821
3.727ArgSer: 3.727 ± 0.611
3.252ArgThr: 3.252 ± 0.608
4.362ArgVal: 4.362 ± 0.492
1.11ArgTrp: 1.11 ± 0.275
1.745ArgTyr: 1.745 ± 0.321
0.0ArgXaa: 0.0 ± 0.0
Ser
6.979SerAla: 6.979 ± 0.604
0.159SerCys: 0.159 ± 0.113
3.727SerAsp: 3.727 ± 0.49
2.459SerGlu: 2.459 ± 0.47
1.507SerPhe: 1.507 ± 0.344
5.948SerGly: 5.948 ± 0.891
1.031SerHis: 1.031 ± 0.316
2.221SerIle: 2.221 ± 0.402
1.824SerLys: 1.824 ± 0.326
5.076SerLeu: 5.076 ± 0.743
1.269SerMet: 1.269 ± 0.286
1.11SerAsn: 1.11 ± 0.295
3.172SerPro: 3.172 ± 0.537
1.903SerGln: 1.903 ± 0.35
3.965SerArg: 3.965 ± 0.589
3.014SerSer: 3.014 ± 0.598
3.014SerThr: 3.014 ± 0.558
3.093SerVal: 3.093 ± 0.554
0.793SerTrp: 0.793 ± 0.326
1.507SerTyr: 1.507 ± 0.428
0.0SerXaa: 0.0 ± 0.0
Thr
6.662ThrAla: 6.662 ± 0.688
0.634ThrCys: 0.634 ± 0.227
4.917ThrAsp: 4.917 ± 0.635
3.41ThrGlu: 3.41 ± 0.731
1.586ThrPhe: 1.586 ± 0.32
6.662ThrGly: 6.662 ± 0.797
1.031ThrHis: 1.031 ± 0.212
3.331ThrIle: 3.331 ± 0.543
1.983ThrLys: 1.983 ± 0.348
4.996ThrLeu: 4.996 ± 0.776
1.11ThrMet: 1.11 ± 0.316
1.903ThrAsn: 1.903 ± 0.542
4.124ThrPro: 4.124 ± 0.452
1.428ThrGln: 1.428 ± 0.34
3.49ThrArg: 3.49 ± 0.458
2.538ThrSer: 2.538 ± 0.595
3.252ThrThr: 3.252 ± 0.718
3.727ThrVal: 3.727 ± 0.542
0.952ThrTrp: 0.952 ± 0.228
1.11ThrTyr: 1.11 ± 0.294
0.0ThrXaa: 0.0 ± 0.0
Val
8.486ValAla: 8.486 ± 1.051
0.476ValCys: 0.476 ± 0.19
2.855ValAsp: 2.855 ± 0.372
4.124ValGlu: 4.124 ± 0.562
2.062ValPhe: 2.062 ± 0.404
3.569ValGly: 3.569 ± 0.525
0.872ValHis: 0.872 ± 0.227
2.776ValIle: 2.776 ± 0.518
2.459ValLys: 2.459 ± 0.573
4.045ValLeu: 4.045 ± 0.64
2.459ValMet: 2.459 ± 0.473
1.586ValAsn: 1.586 ± 0.42
2.459ValPro: 2.459 ± 0.508
2.062ValGln: 2.062 ± 0.345
3.807ValArg: 3.807 ± 0.521
4.362ValSer: 4.362 ± 0.667
4.996ValThr: 4.996 ± 0.597
4.6ValVal: 4.6 ± 0.663
1.428ValTrp: 1.428 ± 0.342
1.745ValTyr: 1.745 ± 0.497
0.0ValXaa: 0.0 ± 0.0
Trp
1.428TrpAla: 1.428 ± 0.344
0.555TrpCys: 0.555 ± 0.204
1.348TrpAsp: 1.348 ± 0.394
0.952TrpGlu: 0.952 ± 0.279
0.634TrpPhe: 0.634 ± 0.286
0.952TrpGly: 0.952 ± 0.269
0.476TrpHis: 0.476 ± 0.198
0.714TrpIle: 0.714 ± 0.23
0.555TrpLys: 0.555 ± 0.187
1.586TrpLeu: 1.586 ± 0.368
0.634TrpMet: 0.634 ± 0.266
0.397TrpAsn: 0.397 ± 0.143
0.952TrpPro: 0.952 ± 0.345
1.428TrpGln: 1.428 ± 0.331
2.141TrpArg: 2.141 ± 0.411
1.269TrpSer: 1.269 ± 0.374
1.507TrpThr: 1.507 ± 0.391
1.11TrpVal: 1.11 ± 0.251
0.238TrpTrp: 0.238 ± 0.156
0.238TrpTyr: 0.238 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.252TyrAla: 3.252 ± 0.512
0.238TyrCys: 0.238 ± 0.127
1.665TyrAsp: 1.665 ± 0.375
1.11TyrGlu: 1.11 ± 0.353
0.555TyrPhe: 0.555 ± 0.22
2.696TyrGly: 2.696 ± 0.496
0.476TyrHis: 0.476 ± 0.184
0.397TyrIle: 0.397 ± 0.159
0.397TyrLys: 0.397 ± 0.149
1.745TyrLeu: 1.745 ± 0.408
0.714TyrMet: 0.714 ± 0.207
1.19TyrAsn: 1.19 ± 0.304
0.793TyrPro: 0.793 ± 0.273
0.952TyrGln: 0.952 ± 0.188
1.745TyrArg: 1.745 ± 0.386
1.031TyrSer: 1.031 ± 0.246
1.428TyrThr: 1.428 ± 0.378
1.428TyrVal: 1.428 ± 0.305
0.634TyrTrp: 0.634 ± 0.186
1.19TyrTyr: 1.19 ± 0.315
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12610 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski